랫드에서 미생탕의 4주 반복투여독성

김태명, 류재면, 서임권, 박동선, 조정희, 장용훈, 김윤배, 황석연 충북대학교 동물의학연구소 2009 Journal of Biomedical and Translational Research Vol.10 No.1

본문참고

해양레저 분야 복합소재 적용: 33피트급 아메리카스컵 훈련용 CFRP 세일링 요트 개발

서형석 ( Hyoung Seock Seo ),장호윤 ( Ho Yun Jang ),이인원 ( In Won Lee ),최흥섭 ( Heung Soap Choi ) 한국복합재료학회 2015 Composites research Vol.28 No.1

본 논문에서는 해양레저분야에서의 현재 복합재 적용 현황과 33피트급 아메리카스컵 훈련용 세일링 요트 개발에 대해 기술한다. 항공분야와 달리 해양레저 분야에서의 복합재료는 최근에서야 적용 및 사용이 되기 시작했다. 다양한 해양레저 구조물 중에서도 특히, 아메리카스컵 세일링 경주 요트는 빠른 속도를 내야 하기 때문에 경량화 및 높은 기계적 성능이 요구되고 이에 따라 경주용 요트를 건조함에 있어 탄소섬유의 사용은 필수적이다. 탄소섬유강화 플라스틱 경주용 요트 제작 과정을 정립하기 위해 탄소 돛대와 탄소 요트 선체에 대한 최적화 설계와 생산 공법에 대해 논의되었다. 최종적으로 이렇게 제작된 탄소섬유강화복합재 세일링 요트는 운항시험을 통해 경주용 요트로써의 높은 성능을 보여주었다. The purpose of this paper is to investigate the current trends of composite applications in the marine and leisure fields and to study the development of 33ft class America’s cup training CFRP sailing yacht. In the field of marine and leisure, composite materials have been just used to marine and leisure structures, recently. Especially, since the America’s cup of sailing yacht racing has required the light weight and high mechanical performance to make a high speed, CFRP have been recognized as the critical material to construct the racing yacht structures. To establish the process of CFRP racing yacht construction, the design optimizations and production methods of carbon mast and CFRP yacht hull were discussed in this paper. Finally, the constructed CFRP sailing yacht exhibited high performance as the racing yacht through the sailing test.

Safety and Feasibility of Laparoscopy-assisted Distal Gastrectomy for Early Gastric Cancer

Hyuk-Joon Lee,Yun-Seock Seo,Moon-Won Yoo,In Kyu Lee,Hyung-Ho Kim,Kuhn Uk Lee,Han-Kwang Yang 대한외과학회 2006 대한외과학회 학술대회 초록집 Vol.2006 No.11

ManBIF: a Program for Mining and Managing Biobank Impact Factor Data

Yu, Ki-Jin,Nam, Jung-Min,Her, Yun,Chu, Min-Seock,Seo, Hyung-Seok,Kim, Jun-Woo,Jeon, Jae-Pil,Park, Hye-Kyung,Park, Kie-Jung Korea Genome Organization 2011 Genomics & informatics Vol.9 No.1

Biobank Impact Factor (BIF), which is a very effective criterion to evaluate the activity of biobanks, can be estimated by the citation information of biobanks from scientific papers. We have developed a program, ManBIF, to investigate the citation information from PDF files in the literature. The program manages a dictionary for expressions to represent biobanks and their resources, mines the citation information by converting PDF files to text files and searching with a dictionary, and produces a statistical report file. It can be used as an important tool by biobanks.

Skin Irritation and Sensitization Studies on Rosin<SUP>®</SUP> Powder

Jae Myun Ryu,Im Kwon Seo,Tae Myoung Kim,Yun-Bae Kim,Si-Kwan Kim,Seung Bok Hong,Seock-Yeon Hwang 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.2

Primary skin irritation and skin sensitization induced by Rosin<SUP>®</SUP>, an extract of pine tree, were assessed in rabbits and guinea pigs, respectively, according to the guidelines of Korea Food and Drug Administration. In primary skin irritation test, 5% and 10% of Rosin<SUP>®</SUP> (in 80% ethanol) exhibited mean score 1.17 and 1.33, respectively, leading to primary irritation indices (PII) of 0.29 and 0.33. Thus, the test compound Rosin<SUP>®</SUP> was evaluated to be a ‘non-irritant’ material. In skin sensitization study using guinea pig maximization test (GPMT), 5% and 10% Rosin<SUP>®</SUP> did not induce erythema or edema, displaying sensitization score 0 and rate 0%, whereas 0.1% 2,4-dinitrochrolobenzene (DNCB), a positive control, induced sensitization scores 1-2 and rate 80%. Therefore, Rosin<SUP>®</SUP> and DNCB were classified to grade Ⅰ and Ⅳ, indicating weak and strong sensitizers, respectively. Taken together, it is suggested that Rosin<SUP>®</SUP> is a pratically-safe material to skin without potential of irritation or allergic sensitization.

Chemopreventive Effects of Magnolia ovobata Extracts on Colonic Aberrant Crypt Foci Induced by 1,2-Dimethylhydrazine in F344 Rats

Jae Myun Ryu,Im Kwon Seo,Tae Myoung Kim,Yun-Bae Kim,Sung Kwon Moon,Kyung-Hwan Jung,Keerang Park,Seung Bok Hong,Seock-Yeon Hwang 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.2

The chemopreventive effects of Magnolia ovobata water extract (MWE) and 70% ethanol extract (MEE) on the development of colonic aberrant crypt foci (ACF) induced by 1,2-dimethylhydrazine (DMH) were investigated. Six-week-old male F344 rats were divided to 7 experimental groups; 1) DMH alone, 2) DMH+0.3% MWE, 3) DMH+1% MWE, 4) DMH+3% MWE, 5) DMH+1% MEE, 6) 3% MWE alone and 7) normal control. Animals were subcutaneously injected with DMH (30 mg/kg) 4 times to induce colonic ACF during the initial 2 weeks, and fed with a basal containing various concentrations of test materials (MWE or MEE) for 8 weeks including the DMH-treatment period. The formation of ACF on colonic mucosa was observed after staining with methylene blue. There are no specific effects of MWE and MEE on body weight, feed and water consumptions, organ weights, histopathological observations, and hematological and blood chemistry analyses. Challenge with DMH alone induced mean number of 270.1 ACF/colon which was somewhat inhibited by MWE treatment, showing average numbers of 234.3-242.1 ACF/colon. In comparison, the ACF number was significantly suppressed to 216.5 by administration of 1% MEE. Therefore, these results suggest that Magnolia ovobata extracts, especially MEE, exert a chemopreventive effect on the DMH-induced colon cancer by inhibiting the early development of ACF.

기후변화에 따른 한국꼬리치레도롱뇽(Onychodactylus koreanus)의 분포 예측에 대한 연구

이수연 ( Su-yeon Lee ),최서윤 ( Seo-yun Choi ),배양섭 ( Yang-seop Bae ),서재화 ( Jae-hwa Suh ),장환진 ( Hoan-jin Jang ),도민석 ( Min-seock Do ) 한국환경생태학회 2021 한국환경생태학회지 Vol.35 No.5

기후변화는 동·식물의 서식지와 개체군을 감소, 소멸시키며, 생물다양성 보존에 위협이 되고 있다. 특히, 도롱뇽과(Hynobiidae)에 속한 종들은 다른 분류군들에 비해 행동권이 작고, 분산 능력이 극히 제한되기 때문에 기후변화에 매우 취약한 분류군이다. 본 연구에서는 한국꼬리치레도롱뇽(Onychodactylus koreanus)의 관찰지점과 종 분포 모델링 기법을 바탕으로 국내 서식하고 있는 한국꼬리치레도롱뇽의 주요 분포지역과 서식특성을 파악하고 기후변화에 따른 분포변화를 예측하였다. 그 결과 고도가 그들의 분포에 가장 주요한 영향을 끼친 환경변수로 확인되었으며, 강원도와 경상북도와 같은 고도가 높은 산림 지역에 밀집된 분포 형태를 보였다. 이처럼 종 분포 모델에서 예측된 공간적 분포 범위와 서식특성은 선행 조사 결과를 충분히 포함하고 있었다. 기후변화에 따른 분포변화를 확인한 결과, 한국꼬리치레 도롱뇽은 현재 분포 범위에 비해 RCP4.5 시나리오에서 62.96% 가 감소할 것으로, RCP8.5 시나리오에서는 98.52% 감소할 것으로 예측되어 기후변화로 인해 서식 적합 공간들이 급격하게 감소하는 것으로 확인되었다. 모델의 AUC 값은 현재에서 0.837, RCP4.5에서 0.832, RCP8.5에서 0.807로 높게 측정되었다. 이러한 결과들은 기후변화로 인해 영향을 받는 양서류의 보전 대책 수립에 중요한 기초자료가 될 수 있을 것이다. 추후, 한국꼬리치레도롱뇽의 생활사에 따른 서식지 특성과 미세한 서식 요인들이 반영된 다양한 분석기법을 통한 추가적인 연구가 수행된다면 종 감소에 영향을 끼치는 주요환경 요인들을 밝혀낼수 있을 것으로 판단된다. Climate change poses great threats to wildlife populations by decreasing their number and destroying their habitats, jeopardizing biodiversity conservation. Asiatic salamander (Hynobiidae) species are particularly vulnerable to climate change due to their small home range and limited dispersal ability. Thus, this study used one salamander species, the Korean clawed salamander (Onychodactylus koreanus), as a model species and examined their habitat characteristics and current distribution in South Korea to predict its spatial distribution under climate change. As a result, we found that altitude was the most important environmental factor for their spatial distribution and that they showed a dense distribution in high-altitude forest regions such as Gangwon and Gyeongsanbuk provinces. The spatial distribution range and habitat characteristics predicted in the species distribution models were sufficiently in accordance with previous studies on the species. By modeling their distribution changes under two different climate change scenarios, we predicted that the distribution range of the Korean clawed salamander population would decrease by 62.96% under the RCP4.5 scenario and by 98.52% under the RCP8.5 scenario, indicating a sharp reduction due to climate change. The model’s AUC value was the highest in the present (0.837), followed by RCP4.5 (0.832) and RCP8.5 (0.807). Our study provides a basic reference for implementing conservation plans for amphibians under climate change. Additional research using various analysis techniques reflecting habitat characteristics and minute habitat factors for the whole life cycle of Korean-tailed salamanders help identify major environmental factors that affect species reduction.

생물전기화학 시스템에서 고농도 질산성 질소 처리를 위한 최적 정전압 조건 평가

김지연 ( Ji Yeon Kim ),서인석 ( In Seock Seo ),김홍석 ( Hong Suck Kim ),윤주환 ( Zu Whan Yun ) 한국물환경학회(구 한국수질보전학회) 2014 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2014 No.-

Four-Week Repeated-Dose Toxicity of Silk Amino Acids in Rats

Tae Myoung Kim,Jae Myun Ryu,Im Kwon Seo,Seongho Yeon,Woo-Taek Lim,Jeong-Yong Lee,Seock-Yeon Hwang,Yun-Bae Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.4

This study was performed to confirm the safety of the silk amino acids (SAA). Male and female Sprague-Dawley rats were orally administered with SAA at dose levels of 500, 1,000 or 2,000 ㎎/㎏ for 4 weeks. There was no significant difference in the body weight gain between vehicle control and SAA-treated rats. Also, significant changes in daily feed and water intakes were not observed. In hematological analysis, SAA decreased the rates of monocytes and eosinophils, while increasing that of lymphocytes, suggestive of reduced inflammatory response and immune potentiation. In particular, SAA significantly increased the numbers of red blood cells, showing microcytic erythropoiesis, and platelets, implying an enhanced hematopoiesis. In blood biochemistry, alanine transaminase, aspartate transaminase and lactate dehydrogenase were significantly reduced by SAA treatment, in contrast to the increases in glucose and albumin. Blood urea nitrogen and creatine phosphokinase also significantly decreased following SAA treatment. In spite of the decreases in the markers of liver, kidneys, skeletal and heart muscles, no remarkable lesions were observed in these organs. Such effects of SAA on the blood markers might be due to the stabilization or protective activities on the active organs, which may lead to enhanced resistance to hyperactivity, hypermotility or excitatory stress. Since no observed adverse-effect level of SAA was found to be higher than the upper-limit dose (2,000 ㎎/㎏), a 20-fold higher level of a clinicallyrecommended dose (100 ㎎/㎏), it is suggested that SAA possess a long-term safety, and that it could be a candidate for the improvement of hematopoiesis and stamina.

Effect of Repeated Administration of Silk Peptide on the Immune System of Rats

Jae Myun Ryu,Tae Myoung Kim,Im Kwon Seo,Seongho Yeon,Woo-Taek Lim,Jeong-Yong Lee,Seock-Yeon Hwang,Namgil O,Jugyeong Song,Jinsoo Lee,Yun-Bae Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.3

In order to elucidate the possibility of influence on the immune system, silk peptide was administered to rats for 4 weeks. Male and female Sprague-Dawley rats were orally treated with low (500 ㎎/㎏) or high (2,000 ㎎/㎏) doses of silk peptide, and complete blood counts, organ weights, histopathology and cellularity of immune systems were examined. Silk peptide did not cause clinical signs, although the body weight gain at the high dose was slightly reduced. In hematological analysis, the number of white blood cells (WBC) and differential counts, the number of red blood cells (RBC) and RBC indices, and the number of platelets and mean platelet volume (MPV) were not affected by the administration of silk peptide. Especially, there were no differences in the number and rate (%) of immune cells such as lymphocytes as well as monocytes and granulocytes between vehicle and silk peptide-treated groups. Silk peptide neither affected the thymus and spleen weights, in addition to the cellularity in thymus, nor induced gross and microscopic lesions of thymus, spleen and lymph node. Taken together, 4-week administration of silk peptide up to 2,000 ㎎/㎏, the upper-limit dose in repeated-dose toxicity study, did not exhibit adverse effects on the immune systems, leading to no observed adverse effect level (NOAEL) higher than 2,000 ㎎/㎏. Without influence on the production, maturation and death process of immune cells at 20-fold dose of clinical dose (100 ㎎/㎏), silk peptide was evaluated to be a practically non-toxic material.