Genome-wide screen identifies novel machineries required for both ciliogenesis and cell cycle arrest upon serum starvation

Kim, J.H.,Ki, S.M.,Joung, J.G.,Scott, E.,Heynen-Genel, S.,Aza-Blanc, P.,Kwon, C.H.,Kim, J.,Gleeson, J.G.,Lee, J.E. Elsevier Biomedical Press 2016 Biochimica et biophysica acta, Molecular cell rese Vol.1863 No.6

Biogenesis of the primary cilium, a cellular organelle mediating various signaling pathways, is generally coordinated with cell cycle exit/re-entry. Although the dynamic cell cycle-associated profile of the primary cilium has been largely accepted, the mechanism governing the link between ciliogenesis and cell cycle progression has been poorly understood. Using a human genome-wide RNAi screen, we identify genes encoding subunits of the spliceosome and proteasome as novel regulators of ciliogenesis. We demonstrate that 1) the mRNA processing-related hits are essential for RNA expression of molecules acting in cilia disassembly, such as AURKA and PLK1, and 2) the ubiquitin-proteasome systems (UPS)-involved hits are necessary for proteolysis of molecules acting in cilia assembly, such as IFT88 and CPAP. In particular, we show that these screen hit-associated mechanisms are crucial for both cilia assembly and cell cycle arrest in response to serum withdrawal. Finally, our data suggest that the mRNA processing mechanism may modulate the UPS-dependent decay of cilia assembly regulators to control ciliary resorption-coupled cell cycle re-entry.

AzTEC half square degree survey of the SHADES fields – I. Maps, catalogues and source counts

Austermann, J. E.,Dunlop, J. S.,Perera, T. A.,Scott, K. S.,Wilson, G. W.,Aretxaga, I.,Hughes, D. H.,Almaini, O.,Chapin, E. L.,Chapman, S. C.,Cirasuolo, M.,Clements, D. L.,Coppin, K. E. K.,Dunne, L.,Dy Blackwell Publishing Ltd 2010 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.401 No.1

<P>ABSTRACT</P><P>We present the first results from the largest deep extragalactic mm-wavelength survey undertaken to date. These results are derived from maps covering over 0.7 deg<SUP>2</SUP>, made at λ= 1.1 mm, using the AzTEC continuum camera mounted on the James Clerk Maxwell Telescope. The maps were made in the two fields originally targeted at λ= 850 μm with the Submillimetre Common-User Bolometer Array (SCUBA) in the SCUBA Half-Degree Extragalactic Survey (SHADES) project, namely the Lockman Hole East (mapped to a depth of 0.9–1.3 mJy rms) and the Subaru/<I>XMM–Newton</I> Deep Field (mapped to a depth of 1.0–1.7 mJy rms). The wealth of existing and forthcoming deep multifrequency data in these two fields will allow the bright mm source population revealed by these new wide-area 1.1 mm images to be explored in detail in subsequent papers. Here, we present the maps themselves, a catalogue of 114 high-significance submillimetre galaxy detections, and a thorough statistical analysis leading to the most robust determination to date of the 1.1 mm source number counts. These new maps, covering an area nearly three times greater than the SCUBA SHADES maps, currently provide the largest sample of cosmological volumes of the high-redshift Universe in the mm or sub-mm. Through careful comparison, we find that both the Cosmic Evolution Survey (COSMOS) and the Great Observatories Origins Deep Survey (GOODS) North fields, also imaged with AzTEC, contain an excess of mm sources over the new 1.1 mm source-count baseline established here. In particular, our new AzTEC/SHADES results indicate that very luminous high-redshift dust enshrouded starbursts (<I>S</I><SUB>1.1mm</SUB> > 3 mJy) are 25–50 per cent less common than would have been inferred from these smaller surveys, thus highlighting the potential roles of cosmic variance and clustering in such measurements. We compare number count predictions from recent models of the evolving mm/sub-mm source population to these sub-mm bright galaxy surveys, which provide important constraints for the ongoing refinement of semi-analytic and hydrodynamical models of galaxy formation, and find that all available models overpredict the number of bright submillimetre galaxies found in this survey.</P>

Gasoline Vehicle Emitted Nanoparticle Characterization

Doh-Won Lee,John M.E. Storey,Jian Wang,Brian H. West,C. Scott Sluder 한국대기환경학회 2006 한국대기환경학회 학술대회논문집 Vol.- No.-

One-year Oxford knee scores should be used in preference to 6-month scores when assessing the outcome of total knee arthroplasty

Clement N. D.,Ng N.,MacDonald D.,Scott C. E. H.,Howie C. R. 대한슬관절학회 2020 대한슬관절학회지 Vol.32 No.-

Purpose: The primary aim of this study was to assess whether there was a clinically significant difference in the mean Oxford knee score (OKS) between 6 and 12 months after total knee arthroplasty (TKA). The secondary aim was to identify variables associated with a clinically significant change in the OKS between 6 and 12 months. Methods: A retrospective cohort study was undertaken using an established arthroplasty database of 1574 primary TKA procedures. Patient demographics, body mass index (BMI), comorbidities, OKS and EuroQoL 5- domain (EQ-5D) score were collected preoperatively and at 6 and 12 months postoperatively. A clinically significant change in the OKS was defined as 5 points or more. Results: There was a 1.1-point increase in the OKS between 6 and 12 months postoperatively, which was statistically significant (95% confidence (CI) 0.8–1.3, p < 0.0001). There were 381 (24.2%) patients who had a clinically significant improvement in their OKS from 6 to 12 months. After adjusting for confounding, patients with a lower BMI (p = 0.028), without diabetes mellitus (p < 0.001), a better preoperative OKS (p < 0.001) or a worse 6-month OKS (p < 0.001) were more likely to have a clinically significant improvement. A 6-month OKS < 36 points was a reliable predictor of a clinically significant improvement in the 6-month to 12-month OKS (area under the curve 0.73, 95% CI 0.70–0.75, p < 0.001). Conclusion: Overall, there was no clinically significant change in the OKS from 6 to 12 months; however, a clinically significant improvement was observed in approximately a quarter of patients and was more likely in those scoring less than 36 points at 6 months. Level of evidence: retrospective diagnostic study, level III.

One-year Oxford knee scores should be used in preference to 6-month scores when assessing the outcome of total knee arthroplasty

( N. D. Clement ),( N. Ng ),( D. MacDonald ),( C. E. H. Scott ),( C. R. Howie ) 대한슬관절학회 2020 대한슬관절학회지 Vol.32 No.-

Purpose: The primary aim of this study was to assess whether there was a clinically significant difference in the mean Oxford knee score (OKS) between 6 and 12 months after total knee arthroplasty (TKA). The secondary aim was to identify variables associated with a clinically significant change in the OKS between 6 and 12 months. Methods: A retrospective cohort study was undertaken using an established arthroplasty database of 1574 primary TKA procedures. Patient demographics, body mass index (BMI), comorbidities, OKS and EuroQoL 5- domain (EQ-5D) score were collected preoperatively and at 6 and 12 months postoperatively. A clinically significant change in the OKS was defined as 5 points or more. Results: There was a 1.1-point increase in the OKS between 6 and 12 months postoperatively, which was statistically significant (95% confidence (CI) 0.8-1.3, p < 0.0001). There were 381 (24.2%) patients who had a clinically significant improvement in their OKS from 6 to 12 months. After adjusting for confounding, patients with a lower BMI (p = 0.028), without diabetes mellitus (p < 0.001), a better preoperative OKS (p < 0.001) or a worse 6-month OKS (p < 0.001) were more likely to have a clinically significant improvement. A 6-month OKS < 36 points was a reliable predictor of a clinically significant improvement in the 6-month to 12-month OKS (area under the curve 0.73, 95% CI 0.70-0.75, p < 0.001). Conclusion: Overall, there was no clinically significant change in the OKS from 6 to 12 months; however, a clinically significant improvement was observed in approximately a quarter of patients and was more likely in those scoring less than 36 points at 6 months. Level of evidence: retrospective diagnostic study, level III.

Improved Functionality of Lithium-Ion Batteries Enabled by Atomic Layer Deposition on the Porous Microstructure of Polymer Separators and Coating Electrodes

Jung, Y. S.,Cavanagh, A. S.,Gedvilas, L.,Widjonarko, N. E.,Scott, I. D.,Lee, S. H.,Kim, G. H.,George, S. M.,Dillon, A. C. Wiley 2012 ADVANCED ENERGY MATERIALS Vol.2 No.8