Simulation and assessment of 99mTc absorbed dose into internal organs from cardiac perfusion scan

Salari Saghar,Khorshidi Abdollah,Soltani-Nabipour Jamshid 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.1

Directly, it is not possible to measure the absorbed dose of radiopharmaceuticals in the organs of the human body. Therefore, simulation methods are utilized to estimate the dose in distinct organs. In this study, individual organs were separately considered as the source organ or target organ to calculate the mean absorption dose, which SAF and S factors were then calculated according to the target uptake via MIRD method. Here, 99mTc activity distribution within the target was analyzed using the definition and simulation of ideal organs by summing the fraction of cumulative activities of the heart as source organ. Thus, GATE code was utilized to simulate the Zubal humanoid phantom. To validate the outcomes in comparison to the similar results reported, the accumulation of activity in the main organs of the body was calculated at the moment of injection and cardiac rest condition after 60 min of injection. The results showed the highest dose absorbed into pancreas was about 21%, then gallbladder 18%, kidney 16%, spleen 15%, heart 8%, liver 8%, thyroid 7%, lungs 5% and brain 2%, respectively, after 1 h of injection. This distinct simulation model may also be used for different periods after injection and modifying the prescribed dose

Verification of an Ad-hoc Serial Communication Protocol through Model-checking: A Case Study with Echo Sounder

Majeed, Shakaiba,Saghar, Kashif,Hameed, Kashif,Ryu, Minsoo International Academy Publishing (IAP) 2017 Journal of software Vol.12 No.4

HOTAIR Long Non-coding RNA: Characterizing the Locus Features by the In Silico Approaches

Mohammadreza Hajjari,Saghar Rahnama 한국유전체학회 2017 Genomics & informatics Vol.15 No.4

HOTAIR is an lncRNA that has been known to have an oncogenic role in different cancers. There is limited knowledge of genetic and epigenetic elements and their interactions for the gene encoding HOTAIR. Therefore, understanding the molecular mechanism and its regulation remains to be challenging. We used different in silico analyses to find genetic and epigenetic elements of HOTAIR gene to gain insight into its regulation. We reported different regulatory elements including canonical promoters, transcription start sites, CpGIs as well as epigenetic marks that are potentially involved in the regulation of HOTAIR gene expression. We identified repeat sequences and single nucleotide polymorphisms that are located within or next to the CpGIs of HOTAIR. Our analyses may help to find potential interactions between genetic and epigenetic elements of HOTAIR gene in the human tissues and show opportunities and limitations for researches on HOTAIR gene in future studies.

Effect of autologous platelet-rich plasma for treatment of recurrent pregnancy loss: a randomized controlled trial

Leila Nazari,Saghar Salehpour,Sedighe Hosseini,Teibeh Hashemi,Nasrin Borumandnia,Elham Azizi 대한산부인과학회 2022 Obstetrics & Gynecology Science Vol.65 No.3

ObjectiveRecurrent pregnancy loss (RPL) is a fertility problem for which no exact mechanism of abortion or efficient treatmenthas been described. This study was conducted between 2018 and 2019 to investigate the effectiveness of autologousplatelet-rich plasma (PRP) in improving the live birth rate of women with RPL who required in vitro fertilization (IVF). MethodsA total of 63 patients with at least two previous pregnancy losses and no specific cause detected for the RPL wereincluded and randomly assigned into two groups (PRP and control). Intrauterine infusion of 0.5 mL of autologous PRPwas performed 48 hours before embryo transfer in the PRP group. Women in the control group received standardtreatment. ResultsForty patients completed the study. The baseline and cycle characteristics of the participants did not differ significantlybetween the PRP and control groups. The clinical pregnancy rate was higher in the PRP group (35% vs. 20%, P=0.288). The live birth rate was 15% in the PRP group, but no live births were recorded in the control group (P=0.231). ConclusionThis is the first study to show that intrauterine infusion of PRP in patients with RPL who undergo IVF may increase thechance of live birth. 영어

HOTAIR Long Non-coding RNA: Characterizing the Locus Features by the In Silico Approaches

Hajjari, Mohammadreza,Rahnama, Saghar Korea Genome Organization 2017 Genomics & informatics Vol.15 No.4

HOTAIR is an lncRNA that has been known to have an oncogenic role in different cancers. There is limited knowledge of genetic and epigenetic elements and their interactions for the gene encoding HOTAIR. Therefore, understanding the molecular mechanism and its regulation remains to be challenging. We used different in silico analyses to find genetic and epigenetic elements of HOTAIR gene to gain insight into its regulation. We reported different regulatory elements including canonical promoters, transcription start sites, CpGIs as well as epigenetic marks that are potentially involved in the regulation of HOTAIR gene expression. We identified repeat sequences and single nucleotide polymorphisms that are located within or next to the CpGIs of HOTAIR. Our analyses may help to find potential interactions between genetic and epigenetic elements of HOTAIR gene in the human tissues and show opportunities and limitations for researches on HOTAIR gene in future studies.

Association of SOD2 rs2758339, rs5746136 and rs2842980 polymorphisms with increased risk of breast cancer: a haplotype-based case–control study

Asadi Sara,Abkar Morteza,Zamanzadeh Zahra,Taghipour Kamalabad Setareh,Sedghi Maryam,Yousefnia Saghar 한국유전학회 2023 Genes & Genomics Vol.45 No.9

Background A growing body of evidence indicates that oxidative stress, high levels of reactive oxygen species (ROS), is implicated in the pathogenesis of breast cancer (BC). Superoxide dismutase (SOD2), a mitochondria-resident antioxidant enzyme, protects cells from ROS by catalytically converting the superoxide radicals into less reactive species. Objective We aimed to investigate whether SOD2 rs2758339, rs5746136 and rs2842980 polymorphisms are associated with increased risk of BC. Methods A total of 100 patients with BC and 100 healthy controls were enrolled in the study. We used polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) assay for genotyping the SOD2 single-nucleotide polymorphisms (SNPs). Under co-dominant, dominant and recessive inheritance models, the genotypic and allelic associations of SOD2 SNPs with susceptibility to BC were evaluated using logistic regression analysis. The haplotype analysis was performed on the SOD2 SNPs to determine their combined effect on the BC risk. Results We found that SOD2 rs5746136 was significantly associated with decreased risk of developing BC in co-dominant and dominant inheritance models (P < 0.05). The SOD2 rs5746136 T allele confers an apparent protective effect against breast carcinogenesis (OR: 1.956; 95% CI 1.312–2.916; P < 0.0001). The SOD2 rs5746136/rs2842980 combined genotypes (CT/AA, CT/AT and TT/AA) were significantly more frequent in healthy subjects compared to BC patients (P < 0.05). The CTA and ACA haplotypes (rs2758339, rs5746136, rs2842980) were found to be a protective and a risk factor for BC, respectively. Conclusion These data strongly suggest that SOD2 rs5746136 was significantly associated with reduced risk of BC, indicating its protective role in BC development.

Increase in concentration of soluble HLA-G in high-quality embryos after intracytoplasmic sperm injection

Mohammad Hassan Heidari,Marefat Ghaffari Novin,Farzaneh Ahmadi,Mehran Khorshid,Tohid Najafi,Aidin Molouki,Saghar Salehpour,Mojgan Bandehpour,Matineh Heidari,Eimi Sato 대한해부학회 2011 Anatomy & Cell Biology Vol.44 No.4

Non-invasive methods are normally preferred to conventional invasive methods when selecting suitable embryos to improve pregnancy rates after assisted reproduction techniques. One of the most recognized non-invasive methods is to examine the supernatants of embryo culture media. Soluble human leukocyte antigen, class I, G (sHLA-G) antigen is a non-classical class I molecule that has been widely considered as a marker of pregnancy failure or implantation success. In the current study of some Iranian patients, we examined the concentration of sHLA-G at different time points after intracytoplasmic sperm injection and compared the rates to the morphology and quality of the selected embryos. We showed that the concentration of sHLA-G increases over time in high-quality embryos. We conclude that there is a positive relationship between morphology, quality, and sHLA-G concentration. We suggest that this relationship can be used to increase the chance of a successful pregnancy.