Quantification of Karanjin, Tannin and Trypsin Inhibitors in Raw and Detoxified Expeller and Solvent Extracted Karanj (Pongamia glabra) Cake

Panda, A.K.,Sastry, V.R.B.,Kumar, A.,Saha, S.K. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.12

Despite being a rich source of protein (28-34%), karanj (Pongamia glabra) cake is found to be bitter in taste and toxic in nature owing to the presence of flavonoid (karanjin), tannin and trypsin inhibitor, thereby restricting its safe inclusion in poultry rations. Feeding of karanj cake at higher levels (>10%) adversely affected the growth performance of poultry due to the presence of these toxic factors. Therefore, efforts were made to detoxify karanj cake by various physico-chemical methods such as dry heat, water washing, pressure cooking, alkali and acid treatments and microbiological treatment with Sacchraromyces cerevisiae (strain S-49). The level of residual karanjin in raw and variously processed cake was quantified by high performance liquid chromatography and tannin and trypsin inhibitor was quantified by titrametric and colorimetric methods, respectively. The karanjin, tannin and trypsin inhibitor levels in such solvent and expeller pressed karanj cake were 0.132, 3.766 and 6.550 and 0.324, 3.172 and 8.513%, respectively. Pressure-cooking of solvent extracted karanj cake (SKC) substantially reduced the karanjin content at a cake:water ratio of 1:0.5 with 30-minute cooking. Among chemical methods, 1.5% (w/w) NaOH was very effective in reducing the karanjin content. $Ca(OH)_2$ treatment was also equally effective in karanjin reduction, but at a higher concentration of 3.0% (w/w). A similar trend was noticed with respect to treatment of expeller pressed karanj cake (EKC). Pressure cooking of EKC was effective in reducing the karanjin level of the cake. Among chemical methods alkali treatment [2% (w/w) NaOH] substantially reduced the karanjin levels of the cake. Other methods such as water washing, dry heat, HCl, glacial acetic acid, urea-ammoniation, combined acid and alkali, and microbiological treatments marginally reduced the karanjin concentration of SKC and EKC. Treatment of both SKC and EKC with 1.5% and 2.0% NaOH (w/w) was the most effective method in reducing the tannin content. Among the various methods of detoxification, dry heat, pressure cooking and microbiological treatment with Saccharomyces cerevisiae were substantially effective in reducing the trypsin inhibitor activity in both SKC and EKC. Based on reduction in karanjin, in addition to tannin and trypsin inhibitor activity, detoxification of SKC with either 1.5% NaOH or 3% $Ca(OH)_2$, w/w) and with 2% NaOH were more effective. Despite the effectiveness of pressure cooking in reducing the karanjin content, it could not be recommended for detoxification because of the practical difficulties in adopting the technology as well as for economic considerations.

Investigations of 3D frustrated S = 5/2 lattice compound KSrFe₂(PO₄)₃

K. Nam,K. Boya,K. Kargeti,A. Jain,R. Kumar,S. K. Panda,S. M. Yusuf,P. L. Paulose,U. K. Voma,E. Kermarrec,Kee Hoon Kim,B. Koteswararao 한국자기학회 2022 한국자기학회 학술연구발표회 논문개요집 Vol.32 No.2

Derivation of Instantaneous Current References for Three Phase PV Inverter Connected to Grid with Active and Reactive Power flow Control

S. Dasgupta,S. N. Mohan,S. K. Sahoo,S. K. Panda 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5

In this paper, inverter reference current generation for a three phase grid connected PV inverter under generalized grid voltage conditions is proposed. The proposed method facilitates high bandwidth grid active and reactive power flow control along with minimum DC link ripples with grid current THD control facility by finding suitable current reference directly in the a-b-c frame. The proposed method is much faster than the conventional methods because of the absence of phase lock loop (PLL) and Park’s transformation requirements. The proposed method is also independent of grid voltage fundamental frequency and capable of rejecting the grid voltage harmonics. These advantages make it more suitable for micro-grid applications. The proposed method is validated with rigorous experiments and test results presented depict the efficacy of the proposed system.

Effects of Dietary Non-phytate Phosphorus Levels on Egg Production, Shell Quality and Nutrient Retention in White Leghorn Layers

Panda, A.K.,Rao, S.V.Rama,Raju, M.V.L.N.,Bhanja, S.K. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.8

An experiment was conducted (28 to 44 weeks) to study the laying performance, shell quality, and nutrient retention of White Leghorn layers fed different levels of non-phytate phosphorus (NPP). Six levels of NPP (0.15, 0.18, 0.21, 0.24, 0.27 and 0.30%) at a constant calcium (Ca) level (3.5%) in maize-soya-deoiled rice bran based diets were formulated, and each experimental diet was offered ad libitum for 16 weeks to five replicates with five birds in each replicate. The body weight of WL layers fed diet containing 0.15% NPP was significantly (p<0.05) lower than those fed diet with 0.30% NPP, at 44 weeks of age. However, the hen day egg production, egg weight, daily feed intake and feed consumed per dozen eggs were not influenced by the variation in the NPP levels in the diet. The bone ash content was significantly (p<0.05) higher in the birds fed 0.30% NPP as compared with those fed diets up to 0.24% NPP. Bone ash content was intermediate in the birds fed diet containing 0.27% NPP. The tibia strength followed the same trend as that of bone ash. Dietary NPP content had no influence on serum Ca and protein concentration and activity of alkaline phosphatase. However, serum inorganic P concentration increased linearly with NPP content in the diet. The concentration of P was significantly (p<0.05) higher in the birds fed 0.27% NPP or higher as compared with those fed 0.15% NPP. Levels of dietary NPP had no influence on egg quality parameters like shell wt, shell thickness, shell strength and specific gravity. The retention of nutrients such as DM, N and Ca were comparable among the WL layers fed different levels of NPP. However, the retention of P decreased linearly with increase in the level of NPP in the diet. The retention of P in the birds fed diets up to 0.24% NPP in the diet was comparable, however further increasing the content of NPP (either 0.27% or 0.30%) reduced the retention of P. Based on the results of the present study, 0.15% NPP (180 mg/b/d) in the diets of WL layers is adequate for optimum production performance during 28 to 44 weeks of age, however, WL layers require 0.27% NPP (324 mg /b/d) in the diet for optimum production with better bone mineralization.

Shear behaviour of RC T-beams strengthenedwith U-wrapped GFRP sheet

K.C.Panda,S. K. Bhattacharyya,S. V. Barai 국제구조공학회 2012 Steel and Composite Structures, An International J Vol.12 No.2

This paper presents an experimental investigation on the performance of 2.5 m long reinforced concrete (RC) T-beams strengthened in shear using epoxy bonded glass fibre fabric. Eighteen (18) full scale, simply supported RC T-beams are tested. Nine beams are used as control beam specimens with three different stirrups spacing without glass fibre reinforced polymer (GFRP) sheet and rest nine beams are strengthened in shear with one, two, and three layers of GFRP sheet in the form of U-jacket around the web of T-beams for each type of stirrup spacing. The objective of this study is to evaluate the effectiveness, the cracking pattern and modes of failure of the GFRP strengthened RC T-beams. The test result indicates that for RC T-beams strengthened in shear with U-jacketed GFRP sheets, increase the load carrying capacity by 10-46%.

Shear strengthening effect by bonded GFRP strips and transverse steel on RC T-beams

K.C. Panda,S.K. Bhattacharyya,S.V. Barai 국제구조공학회 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.47 No.1

This study focuses on shear strengthening performance of simply supported reinforced concrete (RC) T-beams bonded by glass fibre reinforced polymer (GFRP) strips in different configuration, orientations and transverse steel reinforcement in different spacing. Eighteen RC T-beams of 2.5 m span are tested. Nine beams are used as control beam. The stirrups are provided in three different spacing such as without stirrups and with stirrups at a spacing of 200 mm and 300 mm. Another nine beams are used as strengthened beams. GFRP strips are bonded in shear zone in U-shape and side shape with two types of orientation of the strip at 45° and 90° to the longitudinal axis of the beam for each type of stirrup spacing. The experimental result indicates that the beam strengthened with GFRP strips at 45° orientation to the longitudinal axis of the beam are much more effective than 90° orientation. Also as transverse steel increases,the effectiveness of the GFRP strips decreases.

Utilisation of Municipal Waste Water in Aerobic Composting of Solid Organic Waste of Bhubaneswar City

Panda, S.P.,Mishra, C.S.K.,Behera, D.K.,Kim, Jung Wk 서울大學校 環境大學院 2003 環境論叢 Vol.41 No.-

Approximately 600 tons of solid organic wastes and a huge bulk of sewage water are generated in Bhubaneswar city, Orissa, India daily endangering the urban environment. Solid wastes in windrows with sewage water inoculum decomposed faster compared to cow dung slurry and plain water treated wastes. C:N and C:P ratios declined relatively faster in sewage water treated windrows than cow dung slurry and water treated windrows during the experimental period of 45 days. The results indicated that the Bhubaneswar Municipal sewage water would be successfully utilised in solid waste composting.

Early Growth Response of Broilers to Dietary Lysine at Fixed Ratio to Crude Protein and Essential Amino Acids

Panda, A.K.,Rao, S.V. Rama,Raju, M.V.L.N.,Lavanya, G.,Reddy, E. Pradeep Kumar,Sunder, G. Shyam Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.11

The effects of dietary lysine (Lys) at a fixed ratio to crude protein (CP) and essential amino acids (EAA) on early growth response of broilers were studied. Four diets were formulated to contain similar metabolizable energy (ME, 2,950 kcal/kg) but contained graded levels of incremental Lys (1.1, 1.2, 1.3 and 1.4%) while also increasing the dietary CP and EAA (methionine, methionine+ cystine, threonine and tryptophan) to maintain a constant ratio with Lys. Each diet was fed at random to 10 replicates of 6 chicks each throughout the experimental period (1-21 d). At the lowest concentration of Lys of 1.1% (19.04% CP), body weight gain (BWG) was lowest and feed conversion ratio (FCR) was poorest. The BWG increased and FCR decreased linearly as dietary Lys increased upto 1.3% (22.5% CP). Lowest feed consumption was observed in the dietary group that contained 1.1% Lys (19.04% CP) in the diet. Increasing the concentration of Lys to 1.2% (20.77% CP), significantly increased the feed consumption. The concentrations of protein, calcium, phosphorus and cholesterol in serum were not influenced by the variation in Lys contents in the diet. The humoral immune response as measured by antibody titre in response to SRBC inoculation was significantly lower in the diets containing 1.1% Lys compared to 1.4%. It is concluded that the Lys requirement of broilers is 1.3% (22.5% CP) during 0 to 21 days of age for eliciting optimum performance when a fixed ratio of Lys to CP (1:17.31) and essential AA is maintained (1:0.47 Met; 1:0.56 Thr; 1:0.17 Try).

Replacement of Normal Maize with Quality Protein Maize on Performance, Immune Response and Carcass Characteristics of Broiler Chickens

Panda, A.K.,Raju, M.V.L.N.,Rao, S.V. Rama,Lavanya, G.,Reddy, E. Pradeep Kumar,Sunder, G. Shyam Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.12

An experiment was conducted to evaluate the effect of dietary replacement of normal maize (NM) with quality protein maize (QPM) on performance, immune response and carcass characteristics of broiler (Krishibro) chickens. Six experimental diets were prepared separately for starter and finisher phases. Diet 1 was a control diet formulated with NM and soybean meal. In diets 2-5, the NM was replaced with QPM at 25, 50, 75 and 100%, respectively. Diet 6 was the same as the control diet, but supplemented with synthetic lysine similar to the industry standard. Each test diet was fed to 8 replicates, each of 5 chicks, reared in stainless steel battery brooders. The AME content of QPM (3382 kcal/kg) was similar to that of NM (3,352 kcal/kg), but protein (9.91 vs. 8.94%), lysine (0.40 vs. 0.26%) and tryptophan (0.09 vs. 0.07%) contents of QPM were higher than NM. Dietary replacement of NM with 50% QPM significantly (p<0.05) improved body weight gain, feed conversion ratio, humoral immune response, relative bursa weight, and breast muscle yield and lowered abdominal fat content. No further improvement in these parameters was recorded by increasing the level of replacement of NM with QPM to either 75% or 100%. Further, the improvement noticed in the 50% QPM group was similar to the group fed the NM diet with lysine supplementation, and thus dietary replacement of NM with QPM at 50% did not need extra synthetic lysine supplementation. It is concluded that dietary replacement of NM with QPM at the 50% level resulted in optimum performance, higher breast muscle yield and higher immune response in broiler chickens.

Influence of Supplemental Vitamin D₃ on Production Performance of Aged White Leghorn Layer Breeders and Their Progeny

Panda, A.K.,Rao, S.V. Rama,Raju, M.V.L.N.,Niranjan, M.,Reddy, B.L.N. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.11

An experiment was conducted to elucidate the effect of graded levels of vitamin $D_3$ in White Leghorn (WL) layer breeders on egg production, shell quality, hatchability of eggs and juvenile performance of offspring during their late laying period (72-88 wk). White Leghorn breeder females were randomly divided into 5 groups of 50 each and were housed in individual California cages in an open-side housing system. Considering birds in five cages as a replicate, 10 such replicates were randomly allotted to each treatment. A basal diet was formulated containing all the nutrients as recommended for WL layers except vitamin $D_3$, which served as control. Another, four diets were formulated by supplementing graded levels of feed grade crystalline cholecalciferol to the basal diet that contained 300, 600, 1,200 and 2,400 ICU of vitamin $D_3$ per kg. Each diet was offered ad libitum to one of the above five treatment groups. The egg production, egg weight, daily feed consumption and the feed intake per dozen eggs or kg egg mass of the birds fed diet without any supplemental vitamin $D_3$ was comparable with those of supplemental groups. Similarly, the level of vitamin in the diet did not have any effect on any of the above parameters. However, the specific gravity of eggs laid by the birds fed the diet without supplemental vitamin $D_3$ was comparable with either 600 or 2,400 ICU supplemental groups but significantly higher when compared to the 300 and 1,200 ICU groups. The egg -shell breaking strength was significantly lowered in the 600 ICU supplemental groups as compared to the strength of other dietary groups. The Haugh unit, egg shell weight, shell thickness, tibia breaking strength, bone ash and calcium content were not influenced by vitamin $D_3$ concentration in the diet. Serum Ca concentration was influenced by vitamin $D_3$ level in the diet. The serum Ca concentration of birds fed either control or the vitamin supplemented diet up to 1200 ICU/kg diet was comparable. However, increasing the concentration of vitamin $D_3$ to 2,400 ICU/kg diet significantly enhanced the concentration of Ca in the serum, which was significantly higher compared to other dietary groups. The serum concentration of P and protein, however, was not influenced by level of vitamin $D_3$ in the diet. Neither fertility nor hatchability was influenced by vitamin $D_3$ concentration in the diet. Feeding a vitamin $D_3$ deficient diet or supplementation of vitamin to hens did not have any influence on their progeny chicks. It can be concluded that dietary supplementation of vitamin $D_3$ may not be essential for optimum production, shell quality, hatchability, and juvenile performance of WL breeders during 72 to 88 weeks of age.