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Flexural behaviour of Steel Timber Composite (STC) beams
Ruyuan Yang,Haitao Li,Rodolfo Lorenzo,Youfu Sun,Mahmud Ashraf 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.41 No.2
Structural performance of a new type of lightweight steel-timber composite (STC) beam has been investigated by conducting four-point bending tests on 21 specimens. This paper presents key findings on its structural performance parameters such as failure modes, load-deflection response, load-slip response, load-strain response, and the ultimate bending capacity by grouping 21 specimens into 7 subgroups based on various geometric characteristics. In the proposed STC beams, glulam slabs were connected to the steel beams using high-strength bolts, and the effect of different thickness and width of glulam slabs on the structural behaviour of STC beams were carefully investigated. In addition, the effective bending stiffness, deflection, and bending capacity of the STC beams were theoretically calculated based on elastic theory and compared with experimental values. For all considered specimens, timber slabs and steel beams showed good composite action. Increasing the thickness and width of the timber slabs can effectively limit the lateral deformation of the specimens, improve the bending capacity of the specimens, and provides a secant stiffness to the STC beams. It was observed that for the whole cross section of STC beams, the plane section assumption is not applicable, but the strains on timber and steel seemed to satisfy the plane section assumption individually. γ (Gamma) method has been observed to better reflect the deformation capacity of STC beams. Analytical equations were derived to predict the elastic bending capacity of STC beams, and comparison between theoretical and experimental values showed good agreement.
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Yang Gang,Liu Xin,Huang Tianyu,Ding Ruyuan,Wang Yuanyin 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.1
BACKGROUND: Teeth can be used as a raw material for preparing bone substitutes due to their similar chemical composition to bone. The objective of our study was to evaluate the effect of odontogenic biphasic calcium phosphate (BCP) incorporating dentin noncollagenous proteins (DNCPs) on osteogenesis and stability in maxillary sinus augmentation. METHODS: The composition, structure and morphology of the odontogenic BCP were tested by X-ray powder diffraction (XRD), Brunauer–Emmett–Teller, and scanning electron microscopy methods. The biocompatibility and osteoinduction of DNCPs and materials were examined in vitro and their bone regeneration capacity was verified in vivo. RESULTS: The results showed that the cells adhered and proliferated well on the DNCP-loaded BCP scaffold. The odontogenic BCP and DNCPs promoted osteogenic differentiation of cells, The new bone formation in the BCP groups and DNCP subgroups was significantly higher than the new bone formation in the control, and the new bone quality was better. The bone regeneration effect of odontogenic BCP was similar to the effect of deproteinized bovine bone mineral, but b- TCP did not maintain the height and volume of bone reconstruction. CONCLUSION: In conclusion, the combined application of DNCPs and odontogenic BCP is an effective strategy for tissue engineering osteogenesis in the maxillary sinus region. The biomimetic strategy could provide a new approach for patients requiring maxillary sinus lifting.
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Zhu Zhendong,Zhao Haolong,Yang Qitai,Li Yajing,Wang RuYuan,Adetunji Adedeji Olufemi,Min Lingjiang 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.5
Objective: The present study aimed to investigate the effect of β-nicotinamide mononucleotide (NMN) supplementation on ram sperm quality during storage at 4°C in vitro. Methods: Tris-citric acid-glucose solution containing different doses of NMN (0, 30, 60, 90, and 120 μM) was used to dilute semen collected from rams and it was stored at 4°C. Sperm motility, plasma membrane integrity as well as acrosome integrity were evaluated at 0, 24, and 48 h time points after storage at 4°C. In addition, sperm mitochondrial activity, lipid peroxidation (LPO), malondialdehyde (MDA) content, reactive oxygen species (ROS) content, glutathione (GSH) content, superoxide dismutase (SOD) activity, and apoptosis were measured at 48 h time point after storage at 4°C. Results: Results demonstrate that the values obtained for sperm motility, acrosome integrity, and plasma membrane integrity in the NMN treatments were significantly higher than control (p<0.05). The addition of 60 μM NMN significantly improved ram sperm mitochondrial activity and reduced LPO, MDA content, and ROS content compared to control (p<0.05). Interestingly, sperm GSH content and SOD activity for the 60 μM NMN treatment were much higher than those observed for control. NMN treatment also decreased the level of Cleaved-Caspase 3, Cleaved-Caspase 9, and Bax while increasing Bcl-2 level in sperm at 48 h time point after storage at 4°C. Conclusion: Ram sperm quality can be maintained during storage at 4°C with the addition of NMN at 60 μM to the semen extender. NMN also reduces oxidative stress and apoptosis. Overall, these findings suggest that NMN is efficient in improving the viability of ram sperm during storage at 4°C in vitro.
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