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      • KCI등재

        Experimental and Numerical Study on Buckling Behavior of a Rigidly Stiff ened Plate with Tee Ribs

        Lunhua Bai,Ruili Shen,Lu Wang,Yuan Wang,Rusong Miao 한국강구조학회 2018 International Journal of Steel Structures Vol.18 No.2

        In the bridge structures, stiff ened plates are usually designed as rigidly stiff ened when the orthotropic steel box girder is used as the main load-bearing structure. Therefore, the buckling mode of stiff ened plates is plate buckling which occurs in subpanel supported by stiff eners. The orthotropic steel box girder is used as the main girder for Egongyan Rail Special Bridge, which is a self-anchored suspension bridge. Plates of the steel girder are rigidly stiff ened with unequal spacing open ribs, and the most slender stiff ened plate is the mid web stiff ened with Tee ribs. In order to ensure the safety of the bridge, the buckling behavior of the web and orthotropic steel box girder under axial compression, including ultimate strength, post-buckling behavior and failure modes, should be clearly investigated by experimental and numerical methods. The design, loading and testing methods of the 1:4 scale model of the orthotropic steel box girder are introduced in detail fi rstly. The orthotropic steel box girder and the stiff ened web fi nite element (FE) models are validated by the test results, and the eff ects of residual stress and the magnitude of geometric imperfections are discussed roughly. Based on the validated web FE model, a detailed parametric study is performed to systematically investigate the eff ects of residual stress and geometric imperfections on buckling behavior of the web. The eff ect of shapes of geometric imperfections discussed is highlighted. Through tracing stress states, the failure modes of stiff ened plate are in agreement with the experimental phenomenon to some extent. Results show that shapes of geometric imperfections have signifi cantly infl uenced post-buckling behavior and failure modes of the web, but slightly aff ected the ultimate strength. It is advised that residual stress and geometric imperfections should be controlled to make full use of excellent performance of steel materials.

      • SCIESCOPUSKCI등재

        Molecular Cloning, Characterization, and Expression Analysis of Chicken Δ-6 Desaturase

        Kang, Xiangtao,Bai, Yichun,Sun, Guirong,Huang, Yanqun,Chen, Qixin,Han, Ruili,Li, Guoxi,Li, Fadi Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.1

        Long-chain polyunsaturated fatty acids (LC-PUFA) promote the development of brain and vision of the fetus, relieve inflammation, inhibit oral dysplasia of rumor cell, decrease the incidence of cardiovascular disease and regulate arrhythmia. ${\Delta}-6$ desaturase is the rate-limited enzyme in the desaturation process. This study reports the cloning, characterization and tissue expression of a ${\Delta}-6$ desaturase gene in the chicken. PCR primers were designed based on the predicted sequence of chicken ${\Delta}-6$ desaturase (accession number: XM421053) and used to isolate a cDNA fragment of 1,323 bp from chicken liver. Based on the 1,323 bp fragment an EST (BI390105) was obtained by BLAST. The EST and 5'nd of the 1,323 bp fragment were partially overlapped. Gene specific primers derived from the EST were used for amplification of the 5'nd. Another gene-specific primer derived from the 1,323 bp fragment was used for amplification of the 3'nd by 3'ACE. Then the three overlapping cDNA sequences obtained were assembled with DNAMAN software and a full-length ${\Delta}-6$ desaturase of 2,153 bp was obtained. The full-length cDNA contained an ORF of 1,335 bp with a 5'ntranslated region of 147 nucleotides followed by an ATG initiation codon. Stop codon TGA was at position 1,481-1,483 bp. The deduced amino acids shared an homology above 77% with bovine, mice, orangutan, rat and human. The protein sequence had three histidine-rich regions HDFGH (HisI region), HFQHH (HisII region) and HH (HisIII region), a cytochrome $b_{5}$-like domain containing a heme-binding motif and two transmembrane domains. Sequence analysis of the chicken genomic DNA revealed that the coding sequence of chicken ${\Delta}-6$ desaturase included 12 exons and 11 introns. Semi-quantitative RT-PCR showed that the ${\Delta}-6$ desaturase expression levels were in turn liver, spleen, pancreas, lung, breast muscle, heart, and abdominal fat. The expression of ${\Delta}-6$ desaturase in liver was significantly higher than that in breast muscle (p<0.01). The expression of ${\Delta}-6$ desaturase in lung was significantly higher than that in abdominal fat (p<0.01). This is the first clone of chicken ${\Delta}-6$ desaturase.

      • KCI등재

        Fabrication of the Pesticide-Attapulgite composites regulated by mixed-surfactants

        Jiexiang Liu,Ruili Bai,Xiaoguang Zhang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.119 No.-

        The SB (DTAB, TTAB, CTAB, OTAB)-AP complex was obtained by modifying attapulgite (AP) with surfactantof SB (DTAB, TTAB, CTAB, OTAB). The structures were characterized by XRD, FT-IR, TGA/DTA and SEM. A new lamellar structure is formed by introducing surfactant. The basal spacing increases with theincrease of alkyl chain length of cationic surfactants. Then, the beta-cypermethrin (BCT) composites ofBCT/SB-AP, BCT/DTAB (TTAB, CTAB, OTAB)-SB-AP were prepared and investigated. The basal spacingsof BCT composites were larger than those of SB-AP and the corresponding DTAB (TTAB, CTAB, OTAB)-AP. The existence of BCT and surfactants molecules into BCT composites was further confirmed. TheBCT/DTAB (TTAB, CTAB, OTAB)-SB-AP have the smaller specific surface areas than DTAB (TTAB, CTAB,OTAB)-AP due to the increase of AP exfoliation in the presence of SB. Fabrication mechanism of BCT compositeswas put forward. Release behaviors of BCT were investigated and analyzed in different buffersolutions. The existence of mixed-surfactants influenced distinctly the release rates. BCT compositeshad the slow release property. The release behaviors were well fitted by pseudo second-order and parabolicdiffusion models. This study provided a new approach to improve the utilization efficiency of pesticideand could be applied as a pesticide delivery system.

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