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Rugang Yu,Xueling Du,Jing Li,Lan Liu,Chaomeng Hu,Xiaoling Yan,Yuqing Xia,Huijuan Xu 한국유전학회 2020 Genes & Genomics Vol.42 No.4
Background Taproot skin color is a major trait for assessing the commercial and nutritional quality of radish, and red-skinned radish is confirmed to improve consumer’s interest and health. However, little is known about the molecular mechanisms responsible for controlling the formation of red-skinned radish. Objective This study aimed to identify the differentially expressed anthocyanin biosynthetic genes between red- and whiteskinned radishes and understand the molecular regulatory mechanism underlying red-skinned radish formation. Methods Based on the published complete genome sequence of radish, the digital gene expression profiles of Yangzhouyuanbai (YB, white-skinned) and Sading (SD, red-skinned) were analyzed using Illumina sequencing. Results A total of 3666 DEGs were identified in SD compared with YB. Interestingly, 46 genes encoded enzymes related to anthocyanin biosynthesis and 241 genes encoded transcription factors were identified. KEGG pathway analysis showed that the formation of red-skinned radish was mainly controlled by pelargonidin-derived anthocyanin biosynthetic pathway genes. This process included the upregulation of PAL, C4H, 4CL, CHS, CHI, F3H, DFR, LDOX, and UGT enzymes in SD. CHS genes were specifically expressed in SD, and it might be the key point for red pigment accumulation in red-skinned radish. Furthermore, MYB1/2/75, bHLH (TT8), and WD 40 showed higher expression in SD than in YB. Meanwhile, the corresponding low-abundance anthocyanin biosynthesis enzymes and upregulation of MYB4 might be the factors influencing the formation of white-skinned radish. Conclusion These findings provide new insights into the molecular mechanisms and regulatory network of anthocyanin biosynthesis in red-skinned radish.
Zhenyu Xu,Ke Li,Kui Zhou,Shuiyuan Li,Hongwei Chen,Jiaqi Zeng,Rugang Hu 한국섬유공학회 2023 Fibers and polymers Vol.24 No.1
Silk Fibroin (SF) is a protein polymer with great biocompatibility, which can promote cell proliferation and differentiation,and enhance bone repair. In this paper, the effects of distinctive concentrations of SF solutions on the physicochemical andbiological properties of the SF-HA-SA scaffolds were investigated. The SF-HA-SA porous scaffolds were prepared utilizingthe pneumatic extrusion 3D printing technique, composed of hydroxyapatite (HA) and different concentrations of SF solution,and sodium alginate (SA) as a binder. The results shown the SF-HA-SA scaffolds can promote cell proliferation withthe increase of SF concentration in scaffolds, and the strength meets the necessities of trabecular bone defects of bone andcartilage repair. It provides an important reference for the application of SF in bone tissue engineering.