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RISS 인기검색어
- 검색키워드
- 저자 : Reza Darvishzadeh (검색결과 4 건)
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Ali Darvishzadeh,Ali Reza Bandani,Azam Amiri,Seyed Qasem Mousavi 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.3
The red palm weevil Rhynchophorus ferrugineus (Coleoptera: Curculionidae) is a serious pest of date palms in many Middle East countries. The aimof the current study was to obtain a good understanding of the gut proteolytic activity of R. ferrugineus. Thus, the enzymes in the alimentary tract were isolated and their properties were investigated using a general protease substrates (azocasein and hemoglobin) as well as specific substrates of trypsin, chymotrypsin, elastase, cathepsin L, and cathepsin B. Results showed that in the gut of the RPW both serine and cysteine proteases are present although with different activities. Among the active proteases, trypsin was the most active in the gut followed by chymotrypsin and elastase. The activities of these three proteases in the larval gut were 1.32, 1.12 and 0.32 U, respectively. Among cysteine proteases, cathepsin L and cathepsin B were active in the larval gut. The activity of cathepsin L was 0.82 U while that of cathepsin B was 0.31 U. The order of the activity of the proteases in the larval gut was trypsin N chymotrypsin N cathepsin L N elastase N cathepsin B. The effect of pH on general protease substrates hydrolysis showed two distinct peaks: one in the acidic area (pH 6.0) and another in the alkaline area (pH 10.0); these correspond to cysteine and serine protease activities, respectively. Zymogram analysis along with inhibition studies revealed that four proteases are present in the gut.
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Marjan Jannatdoust,Reza Darvishzadeh,Roghayyeh Ziaeifard,Mohammad Ali Ebrahimi,Hamid Hatami Maleki,Esmaeel Gholinezhad,Ali Asghar Hatamnia 한국작물학회 2016 Journal of crop science and biotechnology Vol.19 No.1
Genetic diversity within and among 50 populations of confectionery sunflower (Helianthus annuus L.) collected from different geographical areas of Iran was evaluated by using microsatellite and retrotransposon markers. The number of alleles (Na) in SSR loci ranged from 2 to 3 with an average of 2.1. The polymorphic bands in retrotransposon markers ranged from 7 in locus CR-UR1 to 15 in locus CR-816 with a mean value of 11.33. Herarchical clustering of individuals (50 × 5 = 250) by neighbor joining method in DARwin5 software subdivided them into three groups. Using Bayesian method in the software pakage of Structure, the studied individuals were subdivided into two sub-populations. Principal coordinate analysis revelaed that the two first components explaine 7.86 and 6.16% of the total variance, respectively. Analysis of molecular variance revealed a higher level of genetic variation within (70%) than between (30%) populations. High molecular variation among individuals within population possibly is due to high allogamy nature of the sunflower plant. Low genetic variation observed between populations could be considered as a consequence of genetic equilibrium that has occurred over the long period of cultivation of confectionery sunflower in this area as well as seed exchange among regions. The traditional assumption that selecting genotypes of different geographical origin will maximize the diversity available to a breeding project does not hold in confectionery sunflower.
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Shahabzadeh Zeynab,Darvishzadeh Reza,Mohammadi Reza,Jafari Morad,Alipour Hadi 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.2
Tall fescue (Festuca arundinacea) from Poaceae family is an allohexaploid (2n=6x=42) and cross pollinated plant that is used throughout the world as a forage plant. This research was conducted to identify the genomic regions associated with 10 agro-morphological traits using 15,881 SNP markers in a diverse tall fescue germplasm. UPGMA, Structure, and PCA analyses illustrated high genetic differentiation between forage, turf, and hybrid tall fescue samples. Correcting r2 for population structure and kinship effects revealed the fact that the r2v (corrected for kinship) is much smaller than r2 (uncorrected) illustrating the necessity of removing the effect of coancestry to reduce the inflation of r2 estimates. Analysis of linkage disequilibrium revealed that the amount of SNP pairs in LD differs between sub-genomes and chromosomes and any increase in the distance among SNPs reduces the amount of SNP pairs in LD. A total of 111 and 17 marker-trait associations were identified via GLM and MLM methods for the investigated traits, respectively. Most of the significant MTAs belonged to plant height and days to anthesis. Gene ontology results revealed that 34% of MTAs were located in protein-coding regions. Most of the MTAs were located on chromosomes 1B and 1D. The MTAs for plant height and days to anthesis located within the coding regions can be utilized in genome-based breeding in tall fescue breeding programs.
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