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        Biocontrol activity of volatile organic compounds from Streptomyces alboflavus TD-1 against Aspergillus flavus growth and aflatoxin production

        Mingguan Yang,Laifeng Lu,Jing Pang,Yiling Hu,Qingbin Guo,Zhenjing Li,Shufen Wu,Huanhuan Liu,Changlu Wang 한국미생물학회 2019 The journal of microbiology Vol.57 No.5

        Aspergillus flavus is a saprophytic fungus that contaminates crops with carcinogenic aflatoxin. In the present work, the antifungal effects of volatile organic compounds (VOCs) from Streptomyces alboflavus TD-1 against A. flavus were investigated. VOCs from 8-day-old wheat bran culture of S. alboflavus TD-1 displayed strong inhibitory effects against mycelial growth, sporulation, and conidial germination of A. flavus. Severely misshapen conidia and hyphae of A. flavus were observed by scanning electron microscopy after exposure to VOCs for 6 and 12 h, respectively. Rhodamine 123 staining of mitochondria indicated that mitochondria may be a legitimate antifungal target of the VOCs from S. alboflavus TD-1. Furthermore, the VOCs effectively inhibited aflatoxin B1 production by downregulating genes involved in aflatoxin biosynthesis. Dimethyl trisulfide and benzenamine may play important roles in the suppression of A. flavus growth and production of aflatoxin. The results indicate that VOCs from S. alboflavus TD-1 have tremendous potential to be developed as a useful bio-pesticide for controlling A. flavus.

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        High-efficiency removal of phenol and coking wastewater via photocatalysis-Fenton synergy over a Fe-g-C3N4 graphene hydrogel 3D structure

        Jinshan Hu,Pengfei Zhang,Jifang Cui,Weijia An,Li Liu,Yinghua Liang,Qingbin Yang,Hongjun Yang,Wenquan Cui 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.84 No.-

        To achieve rapid separation of electron-hole pairs and improve photocatalytic degradation activity, theFe-g-C3N4 reduced graphene hydrogel (rGH/Fe-g-C3N4) with a 3D network structure was fabricated viathe hydrothermal method. Using visible light irradiation, H2O2 was added to form a photocatalysis-Fenton synergy system. The results showed that the synergistic degradation rate constant of 10% rGH/Feg-C3N4 was 52% higher than that of the multiphase Fenton reaction and 1.5 times higher than that of theFe-g-C3N4. In the seventh cycle, the catalytic efficiency was still as high as 86.9%. Based on the optimizedconditions from phenol degradation, the system was further applied to coking wastewater treatment,and the degradation efficiency of TOC and COD in 60 min reached 66.3% and 68.1%, respectively. Such highand stable degradation performance was ascribed to the synergy effect of photocatalysis and Fenton. Because of the photogenerated electrons not only can promote the Fe3+/Fe2+ cycle, accelerate thedecomposition of H2O2, but also can quickly transfer to graphene and directly decompose H2O2 to formOH. Thus, a large amount ofOH were generated through the two different channels, which greatlyimprove the degradation efficiency. Furthermore, rGH/Fe-g-C3N4 can be regenerated usingfilters withoutthe need for additional complicated processing. This work provides an effective strategy for the deeptreatment of industrial wastewater.

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        Optimization of ultrasound-assisted magnetic retrieval-linked ionic liquid dispersive liquid–liquid microextraction for the determination of cadmium and lead in water samples by graphite furnace atomic absorption spectrometry

        Li Yao,Xie Wang,Haitao Liu,Chaowen Lin,Liangyu Pang,Junwei Yang,Qingbin Zeng 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.56 No.-

        A simple and efficient method for the determination of Cd and Pb in water samples was developed byultrasound-assisted magnetic retrieval-linked ionic liquid dispersive liquid–liquid microextraction (UA-MR-IL-DLLME) preconcentration and graphite furnace atomic absorption spectrometry (GFAAS)detection. Ionic liquid (IL) [Hmim] [PF6] was used as the extractant, ammonium pyrrolidinedithiocarbamate (APDC) as the complexing agent and Fe3O4 nanoparticles as the sorbent, whileultrasound was applied to assist the dispersion of the extractant and accelerate the mass transfer process. The effect of different factors on the extraction efficiency including pH, IL volume, APDC amount,ultrasound extraction time, ultrasound desorption time and Fe3O4 amount was studied by a fractionalfactorial design to screen for the most important factors. Then a central composite design was used tooptimize the significant factors. Under the optimal conditions, the method has linear calibration curvesover the range of 0.3–20 ng/mL for Cd and 0.5–40 ng/mL for Pb, with correlation coefficients (R) of0.9990 and 0.9986 for Cd and Pb, respectively. The limits of detection were 0.1 ng/mL and 0.15 ng/mL withrelative standard deviations of 3.4% and 2.8% for Cd and Pb, respectively. Finally, the method wassuccessfully applied for the determination of Cd and Pb in the real water samples and satisfactoryrecoveries were achieved.

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