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( Qiang Fu ),( Zi Gong Wei ),( Xiao Hong Liu ),( Ping Ping Xiao ),( Zhao Hui Lu ),( Yao Sheng Chen ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.4
Streptococcus equi ssp. zooepidemicus (Streptococcus zooepidemicus, SEZ) is an important pathogen associated with opportunistic infections of a wide range of species, including pigs and humans. The absence of a suitable vaccine makes it difficult to control SEZ infection. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been previously identified as an immunogenic protein using immunoproteomic techniques. In the present study, we confirmed that the sequence of GAPDH was highly conserved with other Streptococcus spp. The purified recombinant GAPDH could elicit a significant humoral antibody response in mice and confer significant protection against challenge with a lethal dose of SEZ. GAPDH could adhere to the Hep-2 cells, confirmed by flow cytometry, and inhibit adherence of SEZ to Hep-2 cells in an adherence inhibition assay. In addition, real-time PCR demonstrated that GAPDH was induced in vivo following infection of mice with SEZ. These suggest that GAPDH could play an important role in the pathogenesis of SEZ infection and could be a target for vaccination against SEZ.
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Zhao Youqun,Du Xianbin,Lin Fen,Wang Qiang,Fu Hongxun 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.7
To eliminate the potential risk factors of the conventional inflatable tire, a creative non-pneumatic tire named “ME-wheel” was developed. Based on the analysis of the bearing characteristics, four types of ME-wheel with varying hinge structures and distributions were designed. The static stiffness characteristics of these four ME-wheels were investigated by numerical simulations and experiments. The hyperelasticity and incompressibility of the rubber material were described by the Mooney–Rivlin model, and the multilayer rubber-cord composites were modeled by the rebar layer. The nonlinear finite element model of the ME-wheel, which included nonlinear property of the material, contact condition, and anisotropy of rubber-cord composites, was validated by the load characteristic test. Stiffness characteristic tests of these four types of ME-wheel and a pneumatic tire, including vertical, longitudinal, lateral, and torsional stiffness, were carried out using a low speed flatbed test bench. A sufficient comparison and analysis were made between the experimental data and simulation data. The research results provided some theoretical and technical verification on the performance and structural optimization of the ME-wheel.
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Flexural Behavior and Prediction Model of Basalt Fiber/Polypropylene Fiber-Reinforced Concrete
Qiang Fu,Zhaorui Zhang,Wenrui Xu,Xu Zhao,Lu Zhang,Yan Wang,Ditao Niu 한국콘크리트학회 2022 International Journal of Concrete Structures and M Vol.16 No.5
The flexural behavior of basalt fiber (BF)/polypropylene fiber (PF)-reinforced concrete (BPRC) was investigated. When the content of BF and PF is 0.1%, the addition of fibers increases the compressive strength of concrete. A BF content of 0.1% has the most obvious effect on improving the compressive strength, but a hybrid fiber content of 0.2% exhibits a negative effect on the compressive strength. The addition of BF and PF can increase the flexural strength and the expansion tortuosity of the fracture cracks, thus enhancing the ductility of concrete. The hybrid fibers with content of 0.1% are most beneficial to increase the flexural strength. However, the ductility of concrete and the tortuosity of fracture crack decrease with the matrix strength, and the improvement proportion of fibers on the flexural strength also decreases. When the BF and PF are mixed, compared to the case of single fiber added, there is no significant change in the damage of BF, whereas the damage of PF is more severe. The flexural toughness index FTδ effectively characterizes the change in the flexural toughness of BPRC. The hybrid fiber contents of 0.1% and 0.2% exhibit the most significant improving effect on FT-l/600 and FT-l/150, respectively. Considering the influence of fibers on the compressive strength, flexural strength and flexural toughness of concrete, a hybrid content of 0.1% is the optimal choice of fiber content. A prediction model for flexural strength of BPRC is proposed based on the composite material theory.
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MULTIVARIATE EFFECTIVENESS OF ECOLANE AND ECOHMI BASED COOPERATIVE VEHICLE-INFRASTRUCTURE SYSTEM
Qiang Fu,Yiping Wu,Xiaohua Zhao,Yang Bian,Li HaijianLi 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.1
The cooperative vehicle-infrastructure system (CVIS) offers opportunities to further enhance the potential of energy saving and emission reduction for eco-driving. However, it is still not clear about the effectiveness of CVIS on promoting eco-driving nor the multivariate effectiveness of CVIS designed for eco-driving on traffic flow and safety characteristics. A dedicated ecological lane (Ecolane) and ecological human-machine-interaction (EcoHMI) based CVIS (Ecolane-HMI-CVIS) were developed based on driving simulator technology. An experiment of 35 participants was conducted to study eco-driving behavior and final utility to identify the influence of Ecolane-HMI-CVIS. The results indicated that the Ecolane-HMI-CVIS enhanced eco-driving behavior and reduced emission, with CO and NOX significantly reduced by 10.72 % and 9.83 % respectively. The Ecolane-HMI-CVIS reduced the headway and promoted vehicle operation stability, ordering, and improved traffic capacity about 10 %. No negative impact of Ecolane-HMI-CVIS was observed on traffic safety. This study developed a test platform based on driving simulator to explore the multivariate influence of Ecolane-HMI-CVIS. In addition to analyzing the overall effect of Ecolane-HMI-CVIS during the whole experimental section, its detailed influence at each key zone and in spatial change process were also analyzed. This research contributed to better understanding of the working mechanism and effectiveness of Ecolane-HMI-CVIS, and provide technical and policy references of CVIS based eco-driving for traffic management departments.
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Detection of Methanol in Power Transformer Oil Using Spectroscopy
Qiang Fu,Lei Peng,Li Li,Musong Lin,Yaohong Zhao,Shengli Li,Chen Chen 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.2
Oil-paper insulation in transformers plays an important role in the operation of power grid. At present, the aging (life assessment) of transformers are mainly conducted based upon the degree of polymerization, concentration of furfural, and the gas analysis of CO and CO 2 in the transformer oil. However, these techniques lack sensitive response, especially in the early aging of oil-paper insulation. Methanol as a new indicator to evaluate the aging of insulating paper, is getting more and more attention. An evaluation method based on the measurement of methanol concentration using ultraviolet–visible spectrometer was proposed in this work. After the extraction from transformer oils, the methanol was oxidized using potassium permanganate. Then, chromotropic acid was used to react with the oxidation products (formaldehyde) to form a mauve clathrate, fi nally, the methanol content was measured using the spectroscopy. Diff erent from traditional spectroscopy which calculate only by absorbance at the characteristic wavelength, this paper use the partial least squares method, obtain the relationship between the absorbance at selected regions and the concentration of methanol. The minimum detection limit for the proposed method was 10 ppb, whereas the correlation coeffi cient for the relationship between the absorbance and the concentration of methanol was as high as 0.99.
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Research on the Relation between Transformer Oil Flow Electrification and Electrostatic Current
Qiang Fu,Rui Wang,Pinguo Zou,Zhao Li,Yang Yang,Xuejun Xie 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.2
In order to study and obtain the mathematical relation between the electrification degree of transformer oil flow and the electrostatic current, a small amount of data about the electrification degree of oil flow and the corresponding electrostatic current is studied by linear regression method and grey model method. The results show that the linear correlation between the electrification degree and the electrostatic current was not good, and the relation between the electrification degree of oil flow and electrostatic current (i) could be expressed as ρ<SUP>(0)</SUP> =0.2049 i<SUP>(0)</SUP> +169.4419 according to grey model GM (0, 2) when the electrification degree of oil flow is represented by the charge number generated from transformer oil per unit volume, namely the charge density (ρ).
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Lu, Yonggang,Wang, Xiuli,Fu, Qiang,Zhao, Yuanyuan,Zhu, Rongsheng Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.7
Lead alloy is used as coolant in Lead-based cooled Fast Reactor (LFR). The natural characteristics of lead alloy are combined with the simple structural design of LFR. This constitutes the inherent safety characteristics of LFR. The main work of this paper is to take the main coolant pump (MCP) in the lead-cooled fast reactor (LFR) as the research object, and to study the flow pattern distribution of the internal flow field under the reverse rotation pump condition, the reverse rotation positive-flow braking condition and the reverse rotation negative-flow braking condition. In this paper, the double-outlet volute type and the space guide vane are selected as the potential designs of the CLEAR-I MCP. In this paper, the CFD method is used to study the potential reverse accident of the MCP. It is found that the highest flow velocity in the impeller appears at the impeller outlet, and the Q-H curves of the two design programs basically coincide. The space guide vane type MCP has better hydraulic performance under the reverse rotation positive-flow condition, the Q-H curves of the two designs gradually separate with increasing flow rate, and the maximum flow velocity inside the space guide vane type MCP is obviously lower than that of the double-outlet volute type. For the reverse rotation test of MCP, only the condition of the forward rotating pump of the main coolant pump is tested and verified. For the simulation of the MCP in LBE medium, it proved that the turbulence model and basic settings selected in the simulation are reliable.
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