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Park, Changhun,Yun, Seokhun,Lee, Sang Yup,Park, Kyungmoon,Lee, Jinwon Humana Press 2012 Applied biochemistry and biotechnology Vol.167 No.3
<P>The global pool of intracellular metabolites is a reflection of all the metabolic functions of an organism. In the absence of in situ methods capable of directly measuring metabolite pools, intracellular metabolite measurements need to be performed after an extraction procedure. In this study, we evaluated the optimization of technologies for generation of a global metabolomics profile for intracellular metabolites in Klebsiella oxytoca. Intracellular metabolites of K. oxytoca were extracted at the early stationary phase using six different common extraction procedures, including cold methanol, boiling ethanol, methanol/chloroform combinations, hot water, potassium hydroxide, and perchloric acid. The metabolites were subsequently collected for further analysis, and intracellular metabolite concentration profiles were generated using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry. During analysis, the stability of metabolites extracted using cold methanol was clearly higher than that obtained by other extraction methods. For the majority of metabolites, extracts generated in this manner exhibited the greatest recovery, with high reproducibility. Therefore, the use of cold ethanol was the best extraction method for attaining a metabolic profile. However, in another parallel extraction method, perchloric acid may also be required to maximize the range of metabolites recovered, particularly to extract glucose 1-phosphate and NADPH.</P>
( Sol Lee Park ),( Jang Yeon Cho ),( Su Hyun Kim ),( Hong-ju Lee ),( Sang Hyun Kim ),( Min Ju Suh ),( Sion Ham ),( Shashi Kant Bhatia ),( Ranjit Gurav ),( See-hyoung Park ),( Kyungmoon Park ),( Yun-go 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.1
Ever since bioplastics were globally introduced to a wide range of industries, the disposal of used products made with bioplastics has become an issue inseparable from their application. Unlike petroleum-based plastics, bioplastics can be completely decomposed into water and carbon dioxide by microorganisms in a relatively short time, which is an advantage. However, there is little information on the specific degraders and accelerating factors for biodegradation. To elucidate a new strain for biodegrading poly-3-hydroxybutyrate (PHB), we screened out one PHB-degrading bacterium, Microbulbifer sp. SOL03, which is the first reported strain from the Microbulbifer genus to show PHB degradation activity, although Microbulbifer species are known to be complex carbohydrate degraders found in high-salt environments. In this study, we evaluated its biodegradability using solid- and liquid-based methods in addition to examining the changes in physical properties throughout the biodegradation process. Furthermore, we established the optimal conditions for biodegradation with respect to temperature, salt concentration, and additional carbon and nitrogen sources; accordingly, a temperature of 37℃ with the addition of 3% NaCl without additional carbon sources, was determined to be optimal. In summary, we found that Microbulbifer sp. SOL03 showed a PHB degradation yield of almost 97% after 10 days. To the best of our knowledge, this is the first study to investigate the potent bioplastic degradation activity of Microbulbifer sp., and we believe that it can contribute to the development of bioplastics from application to disposal.
꿀벌 탈분 활동 구역과 환경요인에 대한 상관 분석 연구
박보선(Bo-Sun Park),최용수(Yong-Soo Choi),강은진(Eun-Jin Kang),박희근(Heegeun Park),올가 프런제(Olga Frunze),김경문(Kyungmoon Kim),김동원(Dongwon Kim) 한국양봉학회 2021 韓國養蜂學會誌 Vol.36 No.3
This study was conducted to determine the direction and distance of the honeybee feces and assess how feces change depending on external landscape and environmental factors. In order to find out the direction of honeybee feces according to the landscape factors, the experimental area was divided and classified by landscape factors. In addition, honeybee feces experiment according to the climatic factors was conducted eight times per week starting from April 7 to May 26, 2020. As a result, it was confirmed that feces occurred in the direction where the specific gravity of the forest was high. The honeybee feces results showed that the drastically decreased at 300 m, with clear positive relationship with temperature, and negative relationship with wind speed.
Efficacy of pretreating oil palm fronds with an acid—base mixture catalyst
Jung, Young Hoon,Park, Hyun Min,Park, Yong-Cheol,Park, Kyungmoon,Kim, Kyoung Heon Elsevier Applied Science 2017 Bioresource technology Vol.236 No.-
<P><B>Abstract</B></P> <P>Oil palm fronds are abundant but recalcitrant to chemical pretreatment. Herein, an acid—base mixture was applied as a catalyst to efficiently pretreat oil palm fronds. Optimized conditions for the pretreatment were a 0.1M acidic acid—base mixture and 3min ramping to 190°C and 12min holding. The oil palm fronds pretreated and washed with the acid—base mixture exhibited an enzymatic digestibility of 85% by 15 FPU Accellerase 1000/g glucan after 72h hydrolysis, which was significantly higher than the enzymatic digestibilities obtained by acid or alkali pretreatment alone. This could be attributed to the synergistic actions of the acid and base, producing an 87% glucose recovery with 100% and 40.3% removal of xylan and lignin, respectively, from the solids. Therefore, an acid—base mixture can be a feasible catalyst to deconstruct oil palm fronds for sugar production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Oil palm fronds were pretreated with an acid—base mixture catalyst. </LI> <LI> Enzymatic digestibility of pretreated fronds was 85% of the theoretical maximum. </LI> <LI> The acid—base mixture showed pretreatment effects of both acid and base. </LI> </UL> </P>
Bae, Jun Sang,Lee, Jongsung,Park, Yoonkook,Park, Kyungmoon,Kim, Jung Ryul,Cho, Dong Hyu,Jang, Kyu Yun,Park, See-Hyoung Spandidos Publications 2017 Oncology reports Vol.38 No.4
<P>Previously, we reported that auranofin induces apoptosis in SKOV3 cells via regulation of the IKK beta/FOXO3 pathway. In the present study, we reveal that the anticancer activity of auranofin in SKOV3 cells could be enhanced by the attenuation of MUC4 through the regulation of the Her2/Akt/FOXO3 pathway. Compared to the control-siRNA, siRNA transfection against MUC4 into SKOV3 cells accelerated the protein degradation of Her2. Under the same conditions, the expression level of phosphorylated Akt was also downregulated leading to an increase of FOXO3 in the nucleus. Notably, auranofin treatment in SKOV3 cells also resulted in the downregulation of the expression levels of both Her2 and phosphorylated Akt. Thus, Her2 was identified as the common molecular target protein by siRNA transfection against MUC4. Western blot analysis of total and nuclear fraction lysates from SKOV3 cells revealed that attenuation of MUC4 combined with auranofin treatment in SKOV3 cells synergistically activated FOXO3 translocation from the cytoplasm to the nucleus through the regulation of the Her2/Akt/FOXO3 pathway. Attenuation of MUC4 by siRNA transfection potentiated the antitumor effect of auranofin which was examined by performing in vitro assays such as WST-1, cell counting, colony formation, TUNEL and Annexin V staining. In addition, western blot analysis of the apoptosis-related proteins such as PARP1, caspase-3, Bim extra large (EL), Bax and Bcl2 revealed that the attenuation of MUC4 by siRNA transfection potentiates the pro-apoptotic activity of auranofin in SKOV3 cells. Collectively, auranofin could regulate the Her2/Akt/FOXO3 signaling pathway in SKOV3 cells and be used as a potential antitumor agent considering the expression of MUC4 in ovarian cancer patients.</P>