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Kuppusamy, Palaniselvam,Soundharrajan, Ilavenil,Park, Hyung Soo,Kim, Ji Hea,Kim, Won Ho,Jung, Jeong Sung,Choi, Ki Choon The Korean Society of Grassland and Forage Science 2019 한국초지조사료학회지 Vol.39 No.3
The purpose of this study was to analyze the effectiveness of different storage periods of lactic acid bacteria (LAB)-fermented low moisture fresh rice straw silage. The low moisture fresh rice straw sample was inculcated with LAB and stored for different storage periods such as 45, 90, and 365 days, respectively. The low moisture fresh rice straw (LMFRS) silage inoculated with LAB exhibited reduction in pH throughout the fermentation as compared with the control (P<0.05). The lactic acid content was increased at the late fermentation period (90 and 365 days, respectively) in LAB inoculated LMFRS silage as compared with the control (P<0.05). In contrast, the acetic acid and butyric acid concentrations were slightly reduced in the LAB inoculated LMFRS silage sample at 90 and 365 days fermentation, respectively. Meanwhile, the non-inoculated LMFRS silage showed higher amounts of acetic acid and butyric acid at an extended fermentation with low bacterial population as compared with the LAB inoculated LMFRS silage. However, lactic acid concentration was slightly high in the non-inoculated LMFRS silage at early 45 days fermentation. Additionally, the nutrient profile such as crude protein (CP), acid detergent fibre (ADF), neutral detergent fibre (NDF), and total digestibility nutrients (TDN) were not significantly different in control and LAB inculcated samples during all fermentation. Though, the microbial population was greater in the LAB inoculated LMFRS silage as compared with the control. However, the massive population was noted in the LAB inoculated LMFRS silage during all fermentation. It indicates that the inoculated LAB is the main reason for increasing fermentation quality in the sample through pH reduction by organic acids production. Overall results suggest that the LAB inoculums are the effective strain that could be a suitable for LMFRS silage fermentation at prolonged days.
Palaniselvam Kuppusamy,Ilavenil Soundharrajan,Ki Choon Choi 한국초지조사료학회 2019 한국초지조사료학회 학술대회논문집 Vol.2019 No.06
Different types of lactic acid bacteriaw were isolated (LAB) from legume plants. A total of fifty LAB strains were isolated from legume plants and analysed its growth profiles in Alfalfa and crimson clove soups. Results suggested that all strains were able to grow in alfalfa and crimson clove soups. However, only seven strains were growing well and reduced the pH of the soups than the other strains. The selected strains were characterized and identified by biochemical and molecular tools. Results demonstrated that all strains belonged to the Pediococcus pentosaceus. Also, strains can produce industrially important enzymes and ferment the different carbohydrates substrates in-vitro. Overall results suggested that isolated LAB could be considered as potential strains to improve the fermentation process
Kuppusamy, Palaniselvam,Ilavenil, Soundharrajan,Srigopalram, Srisesharam,Maniam, Gaanty Pragas,Yusoff, Mashitah M.,Govindan, Natanamurugaraj,Choi, Ki Choon ELSEVIER 2017 JOURNAL OF CLEANER PRODUCTION Vol.141 No.-
<P><B>Abstract</B></P> <P>Nanoparticles research has been gradually increasing day by day in different aspects due to their potential physico-chemical and biological properties. In the present study, we synthesized ‘green’ copper nanoparticles (CuNPs) using <I>Commelina nudiflora</I> aqueous extract and analysed their biological application on the palm oil mill effluent treatment (POME) and microbial growth control. Synthesized CuNPs were spherical in shape with the average size of 45–100 nm measured by field emission scanning electron microscopy. X-ray diffraction results revealed that the biosynthesized CuNPs were crystalline in nature with higher purity. In addition, we demonstrated the effect of CuNPs on the reduction of COD concentration and microbial populations in the CuNPs treated POME and control samples. The biosynthesized CuNPs were chemically stable with larger surface area and volume ratio resulting significantly reduced the microbial population in CuNPs treated POME. Also, the COD removal efficiency was increased by increasing CuNPs concentrations. The maximum microbial growth control and COD removal were noted at 1000 ppm in the POME waste. The results indicate that CuNPs can be used as a potent source for degrading the organic waste and reducing the microbial growth in the POME waste. Therefore, we suggested that green synthesis of CuNPs could be a major contributor in POME treatment in the near future.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Palaniselvam Kuppusamy,Natanamurugaraj Govindan,Mashitah M. Yusoff,Solachuddin J.A. Ichwan,Narasimha Reddy Parine,Gaanty Pragas Maniam 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.27 No.-
In this study we used common weed plant Commelina nudiflora as reducing and stabilizing agent for goldnanoparticles synthesis. The synthesized gold nanoparticles were characterized using their physical–chemical parameters such as size, shape, composition and crystalline nature by different analyticaltechniques. Biosynthesized gold nanoparticles are spherical and triangle in shapes as reported in thisstudy. The spherical size of the particles ranges between 50 and 150 nm and triangular size is 150 nmas measured by FESEM. The C. nudiflora synthesized gold nanoparticle has potential antibacterial andantioxidant activities.
Ilavenil Soundharrajan,Palaniselvam Kuppusamy,Hyun Jeong Lee,Ki Choon Choi 한국초지조사료학회 2019 한국초지조사료학회 학술대회논문집 Vol.2019 No.06
Lactic acid bacteria (LAB) were isolated and analysed its fermentation ability in triticale powder at different moisture levels. Furthermore, the antibacterial activity of fermented silage extract against cattle pathogens was also studied. The isolated strains were P. pentosaceus (TC48) and L. brevis (TC50) that confirmed based on biochemical and 16srRNA sequences methods. Extract from LAB fermented silage showed higher antibacterial activity (inhibition zone diameters: 18~24.2 mm) against E. coli P. aeruoginosa, S. aureus and E. Fecalis than the non-inoculated silage extract. TC48 and TC52 strains exhibited high tolerance to artificial gastric, duodenal and intestinal fluids. In summary, lactic acid bacteria mediated fermentation of triticale silage extract showed great antibacterial activity with significant probiotic characteristics might be an effective and safe way to provide new strategies for reducing the incidence of pathogenic bacteria associated diseases in animals.
Lactic Acid Bacteria Mixture as Inoculants on Low Moisture Italian Ryegrass Silage Fermentation
Soundharrajan, Ilavenil,Kuppusamy, Palaniselvam,Park, Hyung Soo,Kim, Ji Hea,Kim, Won Ho,Jung, Jeong Sung,Choi, Ki Choon The Korean Society of Grassland and Forage Science 2019 한국초지조사료학회지 Vol.39 No.3
The effects of lactic acid bacteria (LAB) mixtures on low moisture Italian ryegrass (IRG) silage fermentation was evaluated in field conditions. The experiment was categorized into two groups: Un-inoculated (Control) and Inoculated with LAB mixture for four storage periods (45, 90, 180, and 365 days, respectively). Silage inoculated with the LAB mixture had the lowest pH with highest lactic acid production than the control from beginning at 45-365 days at all moistures. Higher LAB counts were observed in inoculated silages than the control silages at whole experimental periods. It is a key reason for the rapid acidification and higher lactic acid production in silages during the storage periods. Overall results suggest that an adding of LAB mixture had positive effects on the increasing aerobic stability of silage and preserved its quality for an extended duration.