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      • SCOPUSKCI등재

        In Vitro Selection of Groundnut Cell Lines from Cercosporidium Personatum Culture Filtrates and Regeneration of Resistant Plants Through Cell Culture

        Venkatachalam, P.,Geetha, N.,Jayabalan, N.,Saravanababu, S. 한국식물학회 1998 Journal of Plant Biology Vol.41 No.4

        Cell suspensions derived from immature leaves of the groundnut (Arachis hypogaea L.) were cultured in the presence and absence of Cercosporidium personation pathotoxic culture filtrates. Cell viability and reactions of cell lines were determined after exposure to various concentrations (25-100%, v/v) of the filtrates. Cell lines have been selected for resistance to the toxin(s) produced by C. personatum. Selected cell lines were used for plant regeneration on regeneration media containing C. personatum culture filtrates. Plant regeneration frequency was found to be low in long-term cultures, whereas it was high in short-term cultures. The selfed progeny of the plants regenerated from the resistant cell lines showed resistance to the pathogen in the field. Six out of 82 plants exhibited enhanced resistance in the R_2 generation. The culture filtrate stimulated callus proliferation as well as plant regeneration at lower concentrations, a response that could prove to be very useful for obtaining disease resistant plants through in vitro selection.

      • An Efficient Plant Regeneration System for Sorghum bicolor - a Valuable Major Cereal Crop

        Baskaran P.,Jayabalan N. The Korean Society of Plant Biotechnology 2005 Plant molecular biology and biotechnology research Vol.7 No.4

        An efficient, rapid and large-scale in vitro clonal propagation of agronomically important Indian cereal crop genotypes (NSH27 & K5) of Sorghum bicolor (L.) Moench. by enhanced shoot proliferation in shoot tip segments was designed. MS medium fortified with plant growth regulators and coconut water markedly influenced in vitro propagation of Sorghum bicolor. In vitro plantlet production system has been investigated on Murashige and Skoog (MS) medium with the synergistic combination of 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), 5% coconut water and 3% sucrose which promoted the maximum number of shoots as well as beneficial shoot length. Subculturing of shoot tip segments on a similar medium enabled continuous production of more than 100 healthy shoots with similar frequency. When the healthy shoot clumps were cultured on MS medium fortified with 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), ${\alpha}$-naphthaleneacetic acid ($2.7\;{\mu}M$), ascorbic acid ($30.0\;{\mu}M$) and 5% coconut water, a rapid production of axillary and adventitious buds was developed after 8 wk culture. More than 300 shoots were produced 10 wk after culture. Rooting was highest (100%) on half strength MS medium containing 22.8 mM IAA. Micropropagated plants established in garden soil, farmyard soil and sand (2:1:1) were uniform and identical to the donor plant with respect to growth characteristics. These plants grew normally without showing any traits.

      • A study on mechanical properties of concrete including activated recycled plastic waste

        Ashok, M.,Jayabalan, P.,Saraswathy, V.,Muralidharan, S. Techno-Press 2020 Advances in concrete construction Vol.9 No.2

        This paper describes the experimental studies carried out to determine the properties of fresh and hardened concrete with Recycled Plastic Waste (RPW) as a partial replacement material for fine aggregates. In the experimental study, RPW was used for replacing river sand and manufactured sand (M sand) aggregates in concrete. The replacement level of fine aggregates was ranging from 5% to 20% by volume with an increment of 5%. M40 grade of concrete with water cement ratio of 0.40 was used in this study. Two different types of RPW were used, and they are (i) un-activated RPW and (ii) activated RPW. The activated RPW was obtained by alkali activation of un-activated RPW using NaOH solution. The hardened properties of the concrete determined were dry density, compressive strength, split tensile strength, flexural strength and ultrasonic pulse velocity (UPV). The properties of the concrete with river sand, M sand, activated RPW and un-activated RPW were compared and inferences were drawn. The effect of activation using NaOH solution was investigated using FT-IR study. The micro structural examination of hardened concrete was carried out using Scanning Electron Microscopy (SEM). The test results show that the strength of concrete with activated RPW was more than that of un-activated RPW. From the results, it is evident that it is feasible to use 5% un-activated RPW and 15% activated RPW as fine aggregates for making concrete without affecting the strength properties.

      • Strength and durability study on cement mortar containing nano materials

        Ashok, M.,Parande, A.K.,Jayabalan, P. Techno-Press 2017 Advances in nano research Vol.5 No.2

        Nano particles have been gaining increasing attention and applied in many fields to fabricate new materials with novel functions due to their unique physical and chemical properties. In the present study two nano materials, namely nano silica (NS) and nano clay metakaolin (NMK) were partially replaced with ordinary Portland cement (OPC). The replacement level was varied from 0.5 to 2.0% in OPC and blended in cement mortar with a water cement ratio of 0.40. Mechanical property studies and durability experiments such as compressive strength, tensile strength, water absorption, depth of chloride penetration test. Nano silica was synthesized from rice husk ash and analyze the size using particle size analyzer. The results indicate that the compressive and tensile strength of the cement mortars containing nano materials were higher strength compared to the plain mortar with the same water cement ratio.

      • KCI등재

        Buckling Behaviour of Hollow Flange Channel Beam Sections in Bending

        R. Senthilkumar,T. S. Sunil,P. Jayabalan 한국강구조학회 2019 International Journal of Steel Structures Vol.19 No.5

        The hollow fl ange beams are designed to take the advantages of hot-rolled steel sections in which area is concentrated away from the neutral axis and the ease of installation associated with cold-formed sections. The present study deals with the fl exural behaviour of hollow fl ange channel beams sections by Finite Element modeling using the general purpose fi nite element software ABAQUS. A Parametric study is carried out by varying the fl at width/thickness ratio, depth of the beam, depth of the hollow fl ange and length of the beam. The eff ects of distortional buckling on hollow fl ange channel beam are studied and fi nite element model results are compared with AS/NZS 4600:2005 code for cold-formed steel structures. It is found that code based design is economic for hollow fl ange beams which fail in lateral distortional buckling alone.

      • KCI등재

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