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- 저자 : Otang, Ntui Valentaine (검색결과 4 건)
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Azadi, Pejman,Otang, Ntui Valentaine,Chin, Dong Poh,Nakamura, Ikuo,Fujisawa, Masaki,Harada, Hisashi,Misawa, Norihiko,Mii, Masahiro The Korean Society of Plant Biotechnology 2010 Plant biotechnology reports Vol.4 No.4
Lilium ${\times}$ formolongi was genetically engineered by Agrobacterium-mediated transformation with the plasmid pCrtZW-N8idi-crtEBIY, which contains seven enzyme genes under the regulation of the CaMV 35S promoter. In the transformants, ketocarotenoids were detected in both calli and leaves, which showed a strong orange color. In transgenic calli, the total amount of carotenoids [133.3 ${\mu}g/g$ fresh weight (FW)] was 26.1-fold higher than in wild-type calli. The chlorophyll content and photosynthetic efficiency in transgenic orange plantlets were significantly lowered; however, after several months of subculture, they had turned into plantlets with green leaves that showed significant increases in chlorophyll and photosynthetic efficiency. The total carotenoid contents in leaves of transgenic orange and green plantlets were quantified at 102.9 and 135.2 ${\mu}g/g$ FW, respectively, corresponding to 5.6- and 7.4-fold increases over the levels in the wild-type. Ketocarotenoids such as echinenone, canthaxanthin, 3'-hydroxyechinenone, 3-hydroxyechinenone, and astaxanthin were detected in both transgenic calli and orange leaves. A significant change in the type and composition of ketocarotenoids was observed during the transition from orange transgenic plantlets to green plantlets. Although 3'-hydroxyechinenone, 3-hydroxyechinenone, astaxanthin, and adonirubin were absent, and echinenone and canthaxanthin were present at lower levels, interestingly, the upregulation of carotenoid biosynthesis led to an increase in the total carotenoid concentration (+31.4%) in leaves of the transgenic green plantlets.
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Kynet Kong,Ikuo Nakamura,So Makabe,Valentine Otang Ntui,Raham Sher Khan 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.2
Fusarium wilt is a soil-borne disease causingsubstantial yield losses in various crops and vegetables. Wehave previously reported the synthetic chitinase (NIC) gene(1.2 kb), in which codon usage of fungus, replaced withthat of plant, conferred resistance against Botrytis cinerea. In this study, the NIC or GUS gene was linked to two rootspecificpromoters, LjNRT2 or AtNRT2.1 (nitrate transporter2), derived from Lotus japonica and Arabidopsisthaliana, respectively. Transgenic tobacco lines expressingLjNRT2-GUS and LjNRT2-NIC, and tomato lines expressingAtNRT2.1-NIC, were produced by Agrobacteriummediatedtransformation. GUS histochemical staining wasobserved in vascular regions of the roots but was conspicuouslyabsent in the leaves of transgenic plants. Westernblot analysis showed the production of NIC proteins inthe roots but not in the leaves of transgenic tobacco andtomato lines. These results indicate that LjNRT2 and At-NRT2.1 promoters expressed transgenes in a root-specificmanner. When in vitro whole plant resistance assay againstFusarium oxysporum was conducted, transgenic plantsshowed increased levels of resistance compared to nontransgenicplants. Antifungal activities of the root extractagainst spore germination of F. oxysporum showed lowerCFU (colony-forming unit) than those of the leaf extract. Root colonization assay against F. oxysporum showedmuch lower CFU values in the roots of transgenic plantsthan in those of non-transgenic plants. These results suggestthat NIC gene triggered by the root-specific promoterssuccessfully expressed only in the roots and conferredincreased levels of resistance against the root pathogen,F. oxysporum.
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Edak Aniedi Uyoh,Ikootobong Sunday Urua,Valentine Otang Ntui,Elza Cletus Okpako 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.4
Nuclear DNA of three accessions of Parkia biglobosa collected from three locations in northern Cross River State was investigated using a Patec PA II flow cytometer equipped with an argon ion laser (488 nm), and pictures of mitotic chromosomes were taken using a digital micro- camera (Canon) placed on the eye piece of a binocular microscope at 100X oil immersion. Metaphase chromosome counts of 2n = 22 for accessions A and C and 2n = 24 for accession B, were obtained and through flow cytometry, the three accessions were confirmed to be diploids. The nuclear DNA content and genome size for the accessions were 1.5085, 1.489, and 1.5266 pg (737.7054, 728.121, and 746.5074 Mbp) for accessions A, B, and C, respectively. In another experiment, leaf samples from greenhouse-germinated seeds were analyzed for variation in the banding pattern of the protein by SDS-PAGE in the three accessions. Protein was resolved into three banding groups according to their electrophoretic mobility: slow, medium, and fast, clustering between 100 - 200, 40 - 70, and 10 - 25 kDa, respectively. There was 76% similarity in the banding pattern between the accessions.
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Uyoh, Edak Aniedi,Urua, Ikootobong Sunday,Ntui, Valentine Otang,Okpako, Elza Cletus 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.4
Nuclear DNA of three accessions of Parkia biglobosa collected from three locations in northern Cross River State was investigated using a Patec PA II flow cytometer equipped with an argon ion laser (488 nm), and pictures of mitotic chromosomes were taken using a digital micro- camera (Canon) placed on the eye piece of a binocular microscope at 100X oil immersion. Metaphase chromosome counts of 2n = 22 for accessions A and C and 2n = 24 for accession B, were obtained and through flow cytometry, the three accessions were confirmed to be diploids. The nuclear DNA content and genome size for the accessions were 1.5085, 1.489, and 1.5266 pg (737.7054, 728.121, and 746.5074 Mbp) for accessions A, B, and C, respectively. In another experiment, leaf samples from greenhouse-germinated seeds were analyzed for variation in the banding pattern of the protein by SDS-PAGE in the three accessions. Protein was resolved into three banding groups according to their electrophoretic mobility: slow, medium, and fast, clustering between 100 - 200, 40 - 70, and 10 - 25 kDa, respectively. There was 76% similarity in the banding pattern between the accessions.
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