In Vitro Antitumor Properties of an Isolate from Leaves of Cassia alata L

Olarte, Elizabeth Iglesias,Herrera, Annabelle Aliga,Villasenor, Irene Manese,Jacinto, Sonia Donaldo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Leaf extracts of Cassia alata L (akapulko), traditionally used for treatment of a variety of diseases, were evaluated for their potential antitumor properties in vitro. MTT assays were used to examine the cytotoxic effects of crude extracts on five human cancer cell lines, namely MCF-7, derived from a breast carcinoma, SK-BR-3, another breast carcinoma, T24 a bladder carcinoma, Col 2, a colorectal carcinoma, and A549, a nonsmall cell lung adenocarcinoma. Hexane extracts showed remarkable cytotoxicity against MCF-7, T24, and Col 2 in a dose-dependent manner. This observation was confirmed by morphological investigation using light microscopy. Further bioassay-directed fractionation of the cytotoxic extract led to the isolation of a TLC-pure isolate labeled as f6l. Isolate f6l was further evaluated using MTT assay and morphological and biochemical investigations, which likewise showed selectivity to MCF-7, T24, and Col 2 cells with $IC_{50}$ values of 16, 17, and 17 ${\mu}g/ml$, respectively. Isolate f6l, however, showed no cytotoxicity towards the non-cancer Chinese hamster ovarian cell line (CHO-AA8). Cytochemical investigation using DAPI staining and biochemical investigation using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-a method used to detect DNA fragmentation-together with caspase assay, demonstrated apoptotic cell death. Spectral characterization of isolate f6l revealed that it contained polyunsaturated fatty acid esters. Considering the cytotoxicity profile and its mode of action, f6l might represent a new promising compound with potential for development as an anticancer drug with low or no toxicity to non-cancer cells used in this study.

Association between immunoglobulin G1 against Tannerella forsythia and reduction in the loss of attachment tissue

Carlos Martín Ardila,Mariana Olarte-Sossa,Isabel Cristina Guzmán 대한치주과학회 2014 Journal of Periodontal & Implant Science Vol.44 No.6

Purpose: To evaluate whether the levels of immunoglobulin G (IgG) antibody to Tanerellaforsythia are associated with periodontal status. Methods: Patients with a diagnosis of chronic periodontitis were considered candidates forthe study; thus 80 chronic periodontitis patients and 28 healthy persons (control group)were invited to participate in this investigation. The presence of T. forsythia was detectedby polymerase chain reaction (PCR) analysis using primers designed to target the respective16S rRNA gene sequences. Peripheral blood was collected from each subject to identify theIgG1 and IgG2 serum antibodies against T. forsythia. All microbiological and immunologicallaboratory processes were completed blindly, without awareness of the clinical status ofthe study patients or of the periodontal sites tested. Results: The bivariate analysis showed that lower mean levels of clinical attachment level(CAL) and probing depth were found in the presence of the IgG1 antibody titers againstwhole-cell T. forsythia; however, only the difference in CAL was statistically significant. Inthe presence of the IgG2 antibody titers against whole-cell T. forsythia, the periodontal parametersevaluated were higher but they did not show statistical differences, except forplaque. The unadjusted linear regression model showed that the IgG1 antibody againstwhole-cell T. forsythia in periodontitis patients was associated with a lower mean CAL(β=–0.654; 95% confidence interval [CI], –1.27 to –0.28; P<0.05). This statistically significantassociation remained after adjusting for possible confounders (β=–0.655; 95% CI,–1.28 to –0.29; P<0.05). On the other hand, smoking was a statistically significant risk factorin the model (β=0.704; 95% CI, 0.24 to 1.38; P<0.05). Conclusions: Significantly lower mean levels of CAL were shown in the presence of theIgG1 antibody titers against whole-cell T. forsythia in periodontitis patients. Thus, the resultsof this study suggest that IgG1 antibody to T. forsythia may have been a protectivefactor from periodontitis in this sample.

Lizeth : Agent Mediated E-Commerce in a Virtural Environment

Osvaldo Cairo,Juan G. Olarte,Fernando E. Rivera 한국지능정보시스템학회 2001 한국지능정보시스템학회 기타 간행물 Vol.- No.-

Association between immunoglobulin G1 against Tannerella forsythia and reduction in the loss of attachment tissue

Ardila, Carlos Martin,Olarte-Sossa, Mariana,Guzman, Isabel Cristina Korean Academy of Periodontology 2014 Journal of Periodontal & Implant Science Vol.44 No.6

Purpose: To evaluate whether the levels of immunoglobulin G (IgG) antibody to Tanerella forsythia are associated with periodontal status. Methods: Patients with a diagnosis of chronic periodontitis were considered candidates for the study; thus 80 chronic periodontitis patients and 28 healthy persons (control group) were invited to participate in this investigation. The presence of T. forsythia was detected by polymerase chain reaction (PCR) analysis using primers designed to target the respective 16S rRNA gene sequences. Peripheral blood was collected from each subject to identify the IgG1 and IgG2 serum antibodies against T. forsythia. All microbiological and immunological laboratory processes were completed blindly, without awareness of the clinical status of the study patients or of the periodontal sites tested. Results: The bivariate analysis showed that lower mean levels of clinical attachment level (CAL) and probing depth were found in the presence of the IgG1 antibody titers against whole-cell T. forsythia; however, only the difference in CAL was statistically significant. In the presence of the IgG2 antibody titers against whole-cell T. forsythia, the periodontal parameters evaluated were higher but they did not show statistical differences, except for plaque. The unadjusted linear regression model showed that the IgG1 antibody against whole-cell T. forsythia in periodontitis patients was associated with a lower mean CAL (${\beta}=-0.654$; 95% confidence interval [CI], -1.27 to -0.28; P<0.05). This statistically significant association remained after adjusting for possible confounders (${\beta}=-0.655$; 95% CI, -1.28 to -0.29; P<0.05). On the other hand, smoking was a statistically significant risk factor in the model (${\beta}=0.704$; 95% CI, 0.24 to 1.38; P<0.05). Conclusions: Significantly lower mean levels of CAL were shown in the presence of the IgG1 antibody titers against whole-cell T. forsythia in periodontitis patients. Thus, the results of this study suggest that IgG1 antibody to T. forsythia may have been a protective factor from periodontitis in this sample.

Abnormal expression of H-Ras induces S-phase arrest and mitotic catastrophe in human T-lymphocyte leukemia

Jorge Antonio Zamora Dominguez,Irma Olarte Carrillo,Rubén Ruiz Ramos,Christian Omar Ramos-Peñafiel,Luis Jiménez Zamudio,Ethel García Latorre,Federico Centeno Cruz,Adolfo Martínez Tovar 대한혈액학회 2023 Blood Research Vol.58 No.1

Background Leukemia is a neoplasm with high incidence and mortality rates. Mitotic death has been observed in tumor cells treated with chemotherapeutic agents. Ras family proteins participate in the transduction of signals involved in different processes, such as proliferation, differentiation, survival, and paradoxically, initiation of cell death. Methods This study investigated the effect of H-Ras expression on human T-cell acute lymphoblastic leukemia MOLT-4 cells. Cells were electroporated with either wild-type (Raswt) or oncogenic mutant in codon 12 exon 1 (Rasmut) versions of H-Ras gene and stained for morphological analysis. Cell viability was assessed using trypan blue staining and cell cycle analysis using flow cytometry. H-Ras gene expression was determined using quantitative real-time reverse transcription polymerase chain reaction. The t, ANOVA, and Scheffe tests were used for statistical analysis. Results Human T-cell acute lymphoblastic leukemia MOLT-4 cells showed nuclear fragmentation and presence of multiple nuclei and micronuclei after transfection with either wt or mutant H-Ras genes. Cell cycle analysis revealed a statistically significant increase in cells in the S phase when transfected with either wt (83.67%, P <0.0005) or mutated (81.79%, P <0.0001) H-Ras genes. Although similar effects for both versions of H-Ras were found, cells transfected with the mutated version died at 120 h of mitotic catastrophe. Conclusion Transfection of human T-cell acute lymphoblastic leukemia MOLT-4 cells with either normal or mutated H-Ras genes induced alterations in morphology, arrest in the S phase, and death by mitotic catastrophe.

INSTAGRAM IMPACT ON DESTINATION MANAGEMENT ORGANIZATION

Maria Elena Aramendia-Muneta,Cristina Olarte-Pascual,Andrea Ollo-L?pez 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07

The purpose of this study is to find out the key to success of the photographs in Instagram through content for tourist destination. While there are several studies in the tourism industry about the impact of Social Networks such as Facebook or virtual collaborative communities like TripAdvisor, there are very few studies about Instagram (Hanan & Putit, 2014). This study focuses on “Beautiful Destinations” as the leader on Instagram and an example of destination management organization (DMO), where they have more than 3,100 publications and 4 million followers in 180 countries, becoming the world’s largest travel influencer on Instagram. The dependent variables are taking from several studies (Dagostar & Isotalo, 1992; Stabler, 1987; Timothy & Groves, 2001). Ordinary least squares models were estimated to assess the relationship of characteristics of the photo with the number of likes among photos published on Instagram. If a photograph has people, water, took during afternoon, self-centric and other centric have effects in the number of likes and comments. While if a destination has the purpose of the engagement of the consumers with several comments, people appearance has a positive impact in the number of comments and if the pictures is doing in the afternoon has a negative impact. By analyzing the contents of information provided by the uploaded photographs, this study provides clues for destination in order to enhance the engagement with potential customer and users of Instagram.

Cardioprotection by Cocoa Polyphenols and ω-3 Fatty Acids: A Disease-Prevention Perspective on Aging-Associated Cardiovascular Risk

Sergio Davinelli,Graziamaria Corbi,Stefano Righetti,Barry Sears,Hector Hugo Olarte,Davide Grassi,Giovanni Scapagnini 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.10

Cardiovascular disease (CVD) remains the leading cause of death today. Many of the biochemical alterations associated with the pathophysiology of CVD can be modified by adequate intakes of bioactive nutrients through a correct diet or supplementation. Recently, there has been growing public and clinical interest in cocoa polyphenols (CPs) and omega-3 (ω-3) fatty acids. A plethora of nutritional intervention trials and experimental studies demonstrates that consumption of these bioactive food compounds is beneficial to promote cardiovascular health. The purpose of this review is to summarize the major cardioprotective effects of CPs and ω-3 fatty acids, providing a scientific rationale for incorporating the combination of these molecules as a nutritional intervention in the prevention of CVD. Although several studies have shown the individual cardioprotective nature of these compounds, a combination treatment with CPs and ω-3 fatty acids may be a promising approach to enhance the preventive value of these molecules and reduce cardiovascular risk factors associated with aging. Therefore, this article also reviews some of the key studies on the interaction between CPs and the metabolism of ω-3 fatty acids.

The rs61764370 Functional Variant in the KRAS Oncogene is Associated with Chronic Myeloid Leukemia Risk in Women

Gutierrez-Malacatt, Humberto,Ayala-Sanchez, Manuel,Aquino-Ortega, Xochitl,Dominguez-Rodriguez, Jacqueline,Martinez-Tovar, Adolfo,Olarte-Carrillo, Irma,Martinez-Hernandez, Angelica,Cecilia, Contreras-C Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Background: Chronic myeloid leukemia (CML) is one of the most frequent hematopoietic malignancies in the elderly population; however, knowledge is limited regarding the genetic factors associated with increased risk for CML. Polymorphisms affecting microRNA (miRNA) biogenesis or mRNA:miRNA interactions are important risk factors in the development of different types of cancer. Thus, we carried out a case-control study to test the association with CML susceptibility of gene variants located in the miRNA machinery genes AGO1 (rs636832) and GEMIN4 (rs2740348), as well as in the miRNA binding sites of the genes BRCA1 (rs799917) and KRAS (rs61764370). Materials and Methods: We determined the genotype of 781 Mexican-Mestizo individuals (469 healthy subjects and 312 CML cases) for the four polymorphisms using TaqMan probes to test the association with CML susceptibility. Results: We found a borderline association of the minor homozygote genotype of the KRAS_rs61764370 polymorphism with an increased risk for CML susceptibility (P = 0.06). After gender stratification, this association was significant only for women (odds ratio [OR] = 13.41, P = 0.04). The distribution of the allelic and genotypic frequencies of the four studied SNPs was neither associated with advanced phases of CML nor treatment response. Conclusions: To the best of our knowledge, this study is the first to show a significant association of the KRAS_rs61764370 SNP with CML. To further determine such an association of with CML susceptibility, our results must be replicated in different ethnic groups.