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Narae Han,Jiwoong Wi,Sungoh Im,Ka-Min Lim,Hun-Dong Lee,Won-Joong Jeong,Geun-Joong Kim,Chan Song Kim,Eun-Jeong Park,Mi Sook Hwang,최동욱 한국조류학회I 2021 ALGAE Vol.36 No.3
An increase in seawater temperature owing to global warming is expected to substantially limit the growth of marine algae, including Pyropia yezoensis, a commercially valuable red alga. To improve our knowledge of the genes involved in the acquisition of heat tolerance in P. yezoensis, transcriptomes sequences were obtained from both the wild-type SG104 P. yezoensis and heat-tolerant mutant Gy500. We selected 1,251 differentially expressed genes that were up- or downregulated in response to the heat stress condition and in the heat-tolerant mutant Gy500, based on fragment per million reads expression values. Among them, PyHRG1 was downregulated under heat stress in SG104 and expressed at a low level in Gy500. PyHRG1 encodes a secretory protein of 26.5 kDa. PyHRG1 shows no significant sequence homology with any known genes deposited in public databases to date. However, PyHRG1 homologs were found in other red algae, including other Pyropia species. When PyHRG1 was introduced into the single-cell green alga Chlamydomonas reinhardtii, transformed cells overexpressing PyHRG1 showed severely retarded growth. These results demonstrate that PyHRG1 encodes a novel red algae-specific protein and plays a role in heat tolerance in algae. The transcriptome sequences obtained in this study, which include PyHRG1, will facilitate future studies to understand the molecular mechanisms involved in heat tolerance in red algae.
Narae Han,Haeyeong Na,Jongkee Kim 한국원예학회 2018 원예과학기술지 Vol.36 No.2
The aim of this study was to determine the concentration of glucoraphenin (GRE) and glucoraphasatin (GRH) from 41 doubled haploid (DH) lines of radish (Raphanus sativus L.) cultivar obtained by isolated microspore culture. Microspores from flower buds (4.0 ± 0.5 mm in length) were cultured in Nitsch and Nitsch medium, and microspore-derived embryos were regenerated into complete plantlets in Murashige and Skoog medium. The average embryo yield was 5.0 ± 2.9 per petri dish. Seeds obtained by bud pollination from the DH lines were planted and leaves of 4-week old seedlings were used for analysis of glucosinolate (GSL) content. In order to determine the GSL content, GRE from seeds and GRH from the roots were extracted and purified to 96.2 and 71.0%, respectively. The amount of GRH markedly varied among the DH lines, ranging from 2.6 to 31.5 mg per gram dry weight. GRH contents were reduced by as much as 14.1% or increased by up to 171.2% compared with the donor plant. There was as much as a 6.1-fold difference in the amount of GRE between the DH lines. Among the 41 DH lines tested, the sum of GRE and GRH contents were significantly reduced in 14 DH lines compared to the donor plant (p = 0.95), whereas the contents were increased in only three DH lines. Our results will be useful for breeding radish genotypes with low and high content of GSL, and the DH lines developed in this study will be useful germplasm for elucidating the regulation of the biosynthesis of aliphatic GSLs in radish plants.
NaRae Han,Jongkee Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Glucosinolates (GLS) are secondary metabolites commonly occurring in Brassica crops and more than 130 different GLS have been reported in diverse plants. Recent studies have indicated that isothiocyanate (ITC) derived from GLS by hydrolysis had a potential for anticancer activity against several rumor cells on human. In addition, it was found that glucoraphenin (GRE) and glucoraphasatin (GRH) were abundant and differently regulated in radish plant, depending upon organs and developmental stages. Microspores isolated from flower buds of radish were cultured in vitro to obtain doubled haploid (DH; but homozygous) lines in a short time period. The present study was conducted to determine the concentration of GRE and GRH, an immediate precursor of ITC from DH lines of radish plant. Total 41 DH lines were selected based on flow cytometry analysis. The seeds, obtained by bud pollination from the DH lines, were planted and 3-weeks-old young seedlings were used for the major aliphatic GLS analysis. Amounts of GRH were highly variable from the DH lines ranging from 2.3 to 31.5 mg·g-1 dry weight (DW). The donor plant (DP) contained 18.4 mg·g-1 DW. It was noticed that there were 6-fold differences in the amounts of GRE between the highest and lowest DH lines. Among 41 lines tested, 14 DH lines of radish plant were significantly reduced in the amount of sum of GRH and GRE compared those of the donor plant (P<0.05), whereas only three lines increased. The results obtained in the present study will lend to select genotypes with low and high GLS contents of radish plant. In addition, those DH lines will aid to elucidate a biosynthetic pathway of the aliphatic GLS in radish plant, which remain for the most part unsolved.