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      • KCI등재

        Interactions between TIME and PIN could play a role in the system that controls the duration of diapause development and synchronization with seasonal cycles in the silkworm Bombyx

        Hidenori KAI,Naoki TANI,Minoru ISOBE 한국곤충학회 2008 Entomological Research Vol.38 No.3

        The significance of winter cold in the termination of diapause was investigated with regards to TIME and PIN in eggs of the silkworm Bombyx mori. TIME (time interval measuring enzyme) is an ATPase that can measure time intervals by exhibiting a transitory burst of activation of the enzyme in accordance with diapause development, which requires cold for resumption of embryonic development in the silkworm. The possible timer function of TIME comprises a built-in mechanism in the protein structure. TIME is a metallo-glycoprotein consisting of 156 amino acid residues with a unique sequence in the N-terminal region to which a sugar chain is attached. PIN (peptidyl inhibitory needle) inhibits the ATPase activity of TIME. PIN is not a simple enzyme inhibitor, but holds the timer by forming a timeregulatory complex with TIME. The carbohydrate moiety of TIME is essential for the assembly of a high-affinity PIN-binding site within the timer motif of the TIME structure. The binding interaction between TIME and PIN was much tighter (nearly 1000 times) at 25°C than that at 4°C, as measured by fluorescence polarization. Because the logEC50 at 4°C was approximately 7 nmol/L, PIN must dissociate from TIME at the physiological concentration of TIME in eggs in the winter cold. Based on the results of our study, we propose that the dissociation of the TIME–-PIN complex in the winter cold cues a series of conformational changes of TIME, ultimately reaching the active form of ATPase which in turn causes the completion of diapause development and initiates new developmental programs.

      • SCIESCOPUSKCI등재

        Immunohistochemical Analysis of Interleukin-17 Producing T Helper Cells and Regulatory T Cells Infiltration in Annular Erythema Associated with Sjogren`s Syndrome

        ( Saori Itoi ),( Atsushi Tanemura ),( Mamori Tani ),( Shun Kitaba ),( Mika Terao ),( Hiroyuki Murota ),( Naoki Oiso ),( Ichiro Katayama ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.2

        Background: Peculiar erythema known as annular erythema associated with Sjogren`s syndrome (AESS) can be differentiated from autoimmune annular erythema and subacute cutaneous lupus erythematosus, both clinically and histologically. However, there are no detailed investigations on immune competent cells infiltration. Objective: Preferential infiltration of interleukin-17-producing T helper (Th17) cells and regulatory T (Treg) cells into the labial salivary gland is reported to play a role in maintaining mucoepithelitis in patients with Sjogren`s syndrome. In this study, we evaluated Th17 and Treg cell infiltration into the lesional skin of AESS. Methods: We analyzed the numbers and infiltration patterns of Th17 and FoxP3 (+) Treg cells in seven cases of AESS using immunohistochemistry. Seven patients with systemic lupus erythematosus (SLE), atopic dermatitis (AD) and psoriasis vulgaris (PV), which are representatives of Th17 cell-involved skin disorders, were enrolled as disease controls. Results: Periappendageal and epidermal changes, such as follicular plugging and liquefaction, were evident in the annular erythema of SLE, not AESS, tissue samples. In AESS tissue samples, dense perivascular and periappendageal in-filtration of lymph cells was observed in the middle-to-deep dermis, as previously described, in contrast to the superficial infiltration pattern observed in both AD and PV samples. While the total number of infiltrated lymphocytes was similar between AESS and SLE tissue samples, Th17 cells were found to be preferentially infiltrated in the middle- to-deep dermis in AESS samples. Conclusion: These results suggest that an increased number and distribution of infiltration of Th17 cells is a preferential feature of AESS, rather than a characteristic feature of annular erythema of SLE.

      • KCI등재

        Selection of dipterocarp species for enrichment planting in a secondary tropical rainforest

        Widiyatno,Fanny Hidayati,Suryo Hardiwinoto,Sapto INDRIOKO,Susilo Purnomo,Jatmoko,Naoki Tani,Mohammad Naiem 한국산림과학회 2020 Forest Science And Technology Vol.16 No.4

        The selection of species for enrichment planting that involves native species in a secondary lowland dipterocarps forest as one of the tropical rainforest types should be conducted to maintain the sustainable management of tropical rainforests. The research of species trial of dipterocarp species was planted under a randomized complete block design with four repli�cations. The following parameters were examined: survival rate, diameter breast height (DBH), pilodyn penetration (PP), and stress wave velocity (SWV). The survival rate (F ¼ 1.72; p ¼ 0.047), DBH (F ¼ 6.67, p < 0.0001), and pilodyn penetration (F ¼ 14.19; p < 0.001) signifi�cantly differed at 12.5 years after planting. The survival rate of Shorea leprosula was the high�est (85%) of all the species, and the survival rate of other species, namely, S. macrophylla, S. ovalis, S. johorensis, S. scaberima, and S. parvifolia, was more than 70%. The best growth of DBH parameter was observed in S. platyclados. The DBH growth of S. platyclados 12.5 years after planting were 27.62 cm (mean annual diameter increment [MADI]¼ 2.2 cm/year). The species whose growth in DBH was similar to those of S. platyclados were S. leprosula, S. parvivolia, and S. ovalis. However, stress wave velocity (SWV) was not significantly different among dipterocarp species (F ¼ 1.26; p ¼ 0.234). Pilodyn penetration (PP) was significantly correlated with DBH (Pearson’s correlations ¼ 0.306; p < 0.001), whereas stress wave velocity was not significantly correlated with DBH and PP (p > 0.05), and their Pearson’s correlations were 0.043 and 0.012, respectively. These results suggested that each dipterocarp species varied in terms of their growth and wood properties. Thus, choosing the species for enrich�ment planting in secondary tropical rainforests depends on the characteristics of each spe�cies and the desired final product of forest management.

      • KCI등재

        Effect of Improved Planting Stock on Tree Growth, Wood Properties, and Soil Fertility of Teak Plantations 10 Years After Planting

        Widiyatno,Aris Wibowo,Dian Novitasari,Gama Widya Seta,Daryono Prehaten,Fanny Hidayati,Widyanto Dwi Nugroho,Suryo Hardiwinoto,Mohammad Naiem,Naoki Tani 한국산림과학회 2024 Forest Science And Technology Vol.20 No.1

        Teak (Tectona grandis Linn. f.) is one of the most economically valuable types of tropical forest because teak wood has high durability, resilience and good aesthetic qualities. Teak tree improvement in Indonesia was initiated in 1983 producing planting stock for reforestation, i.e. clonal seed orchards (CSO) to produce seedling as sexual reproduction (referred to seedling), and hedge orchards to produce shoot cuttings as asexual (vegetative) reproductive (referred to shoot cuttings). Teak plantations established using seedling and shoot cuttings should improve forest productivity. The objectives of this study were to compare the growth rate, wood properties, and soil fertility of teak plantations propagated using seedling and shoot cuttings. Three square plots of 100 trees (10�10 trees; 1,000 m2) were established on both types of plantation and tree growth characteristics, wood properties, and soil fertility were monitored. The tree growth characteristics were diameter at breast height (DBH), tree height (H), clear bole height (CB), and volume of standing stock (Vss), and the wood properties included pilodyn penetration (PP) and stress wave velocity (SWV). Soil fertility was assessed from soil samples collected from three soil depths, with three replicates in each plot: 0–5 cm (top layer), 6–30 cm (middle layer), and 31–60 cm (bottom layer). Soil samples were analysed for total nitrogen, total phosphorus, total potassium, total calcium, and total magnesium. The results showed that the mean DBH, H, CB, and Vss differed significantly between the type of planting stock. The DBH, H, CB, and Vss values were 28.4%, 46.8%, 49.1%, and 130.1% higher, respectively, in teak plantations from shoot cuttings than from seedling. Moreover, the SWV of shoot cuttings planting stock plantation was 3.6% higher than from seedling. The mean PP did not differ significantly between teak plantations from shoot cuttings (22.00 mm) and seedling (21.80 mm). Furthermore, the planting stock did not affect soil fertility, which was highest in the top layer in all treatments. Our results suggest that teak plantations from shoot cuttings would increase growth, forest productivity, maintaining wood quality and soil fertility. Thus, it can maintain sustainable teak forest plantations.

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