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      • 전기분해에 의한 병원폐수의 처리에 관한 연구

        채수권,김남천 서울보건대학 1996 서울보건대학 부설 병원경영연구소 논문집 Vol.2 No.1

        The study on treatment of hospital wastewater was investigated by the electrolytic treatment method using Ti/Pt-PbO electrodes. The batch experimental results were assessed in terms of COD, SS, Br^(-) and F- concentration. Several operating variables, such as the current, pH and amount of NaCl added, were explored to ascertain their respective effects on the treatment efficiency. Optimum operating ranges for each of these operating variables were experimentally determined. The results of this study are as follows. 1. The satisfactory elimination of COD and SS was concurrently achived using Ti/Pt-PbO electrode. The optimum operating conditions were found to be 3ampere, pH 7.5, 5g NaCl/l and electrolytic reaction time of 6~9min. 2. Br^(-) emission rate was increased as a function of current, when operating conditions such as current and electrolytic time maintained inadequately, F^(-) was emitted. So, it was necessary to treat the emitted Br^(-) and F^(-) subsequently.

      • 인터넷을 통한 연구센터 데이터베이스 시스템 구축

        황의천,김일남,김남호,이봉형,왕수현 강원대학교 석재복합신소재제품연구센터 1997 석재연 논문집 Vol.2 No.-

        본 연구의 목적은 석재복합 신소재제품 연구센터의 데이터베이스를 인터넷상에서 구축하여 이 데이터베이스가 연구활동 및 산업지원활동을 지원하도록 하는데 있다. 연구센터와 관련된 데이터베이스를 인터넷상의 세계정보망(world-wide web)이 연계 및 등록시켜 적극적으로 연구소의 활동을 홍보함과 동시에 세계정보망을 통한 각국의 다소 산업체의 산업정보를 실시간(real time)에 검색할 수 있는 정보망을 구축할 것이다. 석재복합 신소재제품 연구센터의 연구활동, 연구결과와 관련 산업체의 활동들을 데이터베이스화한 뒤, 이를 인터넷상에 제공함으로써 연구소의 연구활동 및 관련산업체들의 활동을 국제적으로 홍보하며 각국의 수요와 온라인으로 상담 및 전자우편을 나눌 수 있으므로 해서 지역경제의 국제화를 맞을 것으로 기대된다. 또한 앞으로 있을 정보고속도로의 구축과 활용을 위하여 본 연구는 기여하게 될 것이다. The purpose of this study is to construct the database of RRC research center on internet to support the research activities and industrial activities. Registering the database on internet, the center will proactively advertise the local industrial activities and will construct the information network which will enable the real-time search for industrial information. The database will include RRC's research activities, research output, and related industrial's activities. The homepage will advertise the RRC's activities worldwidely, support the online business, provide the e-mail, and it will enable the research center to be the leader of internationalization of local economy. The study will also contribute to further study of information highway.

      • 돌연변이성에 의한 수중유기 오염물질의 독성평가

        유영식,채수권,권우택,김남천 서울보건대학 1996 서울보건대학 부설 한국보건과학연구소 논문집 Vol.3 No.1

        한강수계 팔당댐 유입 하천수를 대상으로, 다환방향족 탄화수소를 선택적으로 유리하게 흡착하는 blue-rayon을 이용하는 현수법 및 column법으로 유기물을 흡착 추출하여, Salmonella typhimurium TA 98 및 TA 100에서의 돌연변이원성을 검색한 결과 다음과 같았다. 1. 흡착 유기물량은 8월이 11월에 비하여 약 1.7배 높았고, 현수법과 column법에서의 차는 없었다. 2. TA 100 균주에서 돌연변이원성은 나타나지 않았다. 3. TA 98균주에서의 돌연변이원성은, S-9mix 첨가의 계가 S-9mix비첨가의 계보다 높았고, 11월이 8월보다 높게 나타났다. Column법이 현수법에 비하여 돌연변이원성은 예민하게 검출되었다. In order to evaluate the toxic effect of organic pollutants in aquatic environments, organic substances extracted from blue-rayon adsorbates in Kyimgan River with the hanging and column methods were assayed by the Ames Salmonella mutagenicity test. Amounts of organic extracts adsorbed with the blue-rayon hanging method and the blue-rayon column method were similar. The mutagenicity toward TA 100 strain was not shown. The mutagenicity toward TA 98 with S-9mix was higher than that without S-9mix. For the quantitative concentration of polycyclic aromatic hydrocarbons in water, it was shown that the blue-rayon column method was more efficient than the blue-rayon hanging method.

      • Natural Variation in OsPRR37 Regulates Heading Date and Contributes to Rice Cultivation at a Wide Range of Latitudes

        Soo-Cheul Yoo,Bon-Hyuk Koo,Choon-Tak Kwon,Nam-Chon Paek 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

        Heading date and photoperiod sensitivity are fundamental traits that determine rice adaptation to a wide range of geographic environments. By quantitative trait locus (QTL) mapping and candidate gene analysis using wholegenome re-sequencing, we found that Oryza sativa Pseudo-Response Regulator37 (OsPRR37; hereafter PRR37) is responsible for the Early heading7-2 (EH7-2)/Heading date2 (Hd2) QTL which was identified from a cross of late-heading rice ‘Milyang23 (M23)’ and early-heading rice ‘H143’. H143 contains a missense mutation of an invariantly conserved amino acid in the CCT (CONSTANS, CO-like, and TOC1) domain of PRR37 protein. In the world rice collection, different types of nonfunctional PRR37 alleles were found in many European and Asian rice cultivars. Notably, the japonica varieties harboring nonfunctional alleles of both Ghd7/Hd4 and PRR37/Hd2 flower extremely early under natural long-day conditions, and are adapted to the northernmost regions of rice cultivation, up to 53° N latitude. Genetic analysis revealed that the effects of PRR37 and Ghd7 alleles on heading date are additive, and PRR37 down-regulates Hd3a expression to suppress flowering under long-day conditions. Our results demonstrate that natural variations in PRR37/Hd2 and Ghd7/Hd4 have contributed to the expansion of rice cultivation to temperate and cooler regions

      • Rice FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (OsFKF1) promotes flowering independent of photoperiod.

        Su-Hyun Han,Soo-Cheul Yoo,Nam-Chon Paek 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

        In the facultative long-day (LD) plant Arabidopsis thaliana, FLAVIN-BINDING, KELCH REPEAT, F-BOX1 (FKF1) is activated by blue light and promotes flowering through the transcriptional and post-transcriptional regulation of CONSTANS under inductive LD conditions. By contrast, the facultative short day (SD) plant rice (Oryza sativa) flowers early under inductive SD and late under non-inductive LD conditions; the regulatory function of OsFKF1 remains elusive. Here we show that osfkf1 mutants flower late under SD, LD, and natural LD conditions. Transcriptional analysis revealed that OsFKF1 up-regulates expression of the floral activator Ehd2 and down-regulates expression of the floral repressor Ghd7; these regulators up- and down-regulate Ehd1 expression, respectively. Moreover, OsFKF1 can upregulate Ehd1 expression under blue light treatment, without affecting the expression of Ehd2 and Ghd7. In contrast to the LD-specific floral activator Arabidopsis FKF1, OsFKF1 likely acts as an autonomous floral activator because it promotes flowering independent of photoperiod, probably via its distinct roles in controlling expression of rice-specific genes including Ehd2, Ghd7, and Ehd1. Like Arabidopsis FKF1, which interacts with GI and CDF1, OsFKF1 also interacts with OsGI and OsCDF1 (also termed OsDOF12). Thus, we have identified similar and distinct roles of FKF1 in Arabidopsis and rice.

      • Rice virescent3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development.

        Yoo, Soo-Cheul,Cho, Sung-Hwan,Sugimoto, Hiroki,Li, Jinjie,Kusumi, Kensuke,Koh, Hee-Jong,Iba, Koh,Paek, Nam-Chon American Society of Plant Physiologists 2009 PLANT PHYSIOLOGY - Vol.150 No.1

        <P>The virescent3 (v3) and stripe1 (st1) mutants in rice (Oryza sativa) produce chlorotic leaves in a growth stage-dependent manner under field conditions. They are temperature-conditional mutants that produce bleached leaves at a constant 20 degrees C or 30 degrees C but almost green leaves under diurnal 30 degrees C/20 degrees C conditions. Here, we show V3 and St1, which encode the large and small subunits of ribonucleotide reductase (RNR), RNRL1, and RNRS1, respectively. RNR regulates the rate of deoxyribonucleotide production for DNA synthesis and repair. RNRL1 and RNRS1 are highly expressed in the shoot base and in young leaves, and the expression of the genes that function in plastid transcription/translation and in photosynthesis is altered in v3 and st1 mutants, indicating that a threshold activity of RNR is required for chloroplast biogenesis in developing leaves. There are additional RNR homologs in rice, RNRL2 and RNRS2, and eukaryotic RNRs comprise alpha(2)beta(2) heterodimers. In yeast, RNRL1 interacts with RNRS1 (RNRL1:RNRS1) and RNRL2:RNRS2, but no interaction occurs between other combinations of the large and small subunits. The interacting activities are RNRL1:RNRS1 > RNRL1:rnrs1(st1) > rnrl1(v3):RNRS1 > rnrl1(v3):rnrs1(st1), which correlate with the degree of chlorosis for each genotype. This suggests that missense mutations in rnrl1(v3) and rnrs1(st1) attenuate the first alphabeta dimerization. Moreover, wild-type plants exposed to a low concentration of an RNR inhibitor, hydroxyurea, produce chlorotic leaves without growth retardation, reminiscent of v3 and st1 mutants. We thus propose that upon insufficient activity of RNR, plastid DNA synthesis is preferentially arrested to allow nuclear genome replication in developing leaves, leading to continuous plant growth.</P>

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        The Hypernodulating nts mutation induces jasmonate synthetic pathway in soybean leaves.

        Seo, Hak Soo,Li, Jinjie,Lee, Sun-Young,Yu, Jae-Woong,Kim, Kil-Hyun,Lee, Suk-Ha,Lee, In-Jung,Paek, Nam-Chon Korean Society for Molecular Biology 2007 Molecules and cells Vol.24 No.2

        <P>Symbiotic nitrogen fixation with nitrogen-fixing bacteria in the root nodules is a distinctly beneficial metabolic process in legume plants. Legumes control the nodule number and nodulation zone through a systemic negative regulatory system between shoot and root. Mutation in the soybean NTS gene encoding GmNARK, a CLAVATA1-like serine/threonine receptor-like kinase, causes excessive nodule development called hypernodulation. To examine the effect of nts mutation on the gene expression profile in the leaves, suppression subtractive hybridization was performed with the trifoliate leaves of nts mutant 'SS2-2' and the wild-type (WT) parent Sinpaldalkong2, and 75 EST clones that were highly expressed in the leaves of the SS2-2 mutant were identified. Interestingly, the expression of jasmonate (JA)-responsive genes such as vspA, vspB, and Lox2 were upregulated, whereas that of a salicylate-responsive gene PR1a was suppressed in the SS2-2 mutant. In addition, the level of JA was about two-fold higher in the leaves of the SS2-2 mutant than in those of the WT under natural growth conditions. Moreover, the JA-responsive gene expression persists in the leaves of SS2-2 mutant without rhizobia infection in the roots. Taken together, our results suggest that the nts mutation increases JA synthesis in mature leaves and consequently leads to constitutive expression of JA-responsive genes which is irrelevant to hypernodulation in the root.</P>

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