Characterization of Solanum tuberosum multicystatin and its structural comparison with other cystatins.

Nissen, Mark S,Kumar, G N Mohan,Youn, Buhyun,Knowles, D Benjamin,Lam, Ka Sum,Ballinger, W Jordan,Knowles, N Richard,Kang, Chulhee American Society of Plant Physiologists 2009 The Plant cell Vol.21 No.3

<P>Potato (Solanum tuberosum) multicystatin (PMC) is a crystalline Cys protease inhibitor present in the subphellogen layer of potato tubers. It consists of eight tandem domains of similar size and sequence. Our in vitro results showed that the pH/PO(4)(-)-dependent oligomeric behavior of PMC was due to its multidomain nature and was not a characteristic of the individual domains. Using a single domain of PMC, which still maintains inhibitor activity, we identified a target protein of PMC, a putative Cys protease. In addition, our crystal structure of a representative repeating unit of PMC, PMC-2, showed structural similarity to both type I and type II cystatins. The N-terminal trunk, alpha-helix, and L2 region of PMC-2 were most similar to those of type I cystatins, while the conformation of L1 more closely resembled that of type II cystatins. The structure of PMC-2 was most similar to the intensely sweet protein monellin from Dioscorephyllum cumminisii (serendipity berry), despite a low level of sequence similarity. We present a model for the possible molecular organization of the eight inhibitory domains in crystalline PMC. The unique molecular properties of the oligomeric PMC crystal are discussed in relation to its potential function in regulating the activity of proteases in potato tubers.</P>

ON REDUCTION OF GTP TUNNELS FOR MBMS DATA TRANSFER IN UMTS

Rama Mohan Babu K N,K.N.Balasubramanya Murthy,A Srinivas,Mahesh Kumar BM 보안공학연구지원센터 2011 International Journal of Future Generation Communi Vol.4 No.1

Relative Microalgal Concentration in Prydz Bay, East Antarctica during Late Austral Summer, 2006

Mohan, Rahul,Shukla, Sunil Kumar,Anilkumar, N.,Sudhakar, M.,Prakash, Satya,Ramesh, R. The Korean Society of Phycology 2009 ALGAE Vol.24 No.3

Microalgae using a submersible fluorescence probe in water column (up to 100 m) were measured during the austral summer of 2006 (February) in Prydz Bay, East Antarctica (triangular-shaped embayment in the Indian sector of Southern Ocean). Concurrently, environmental parameters such as temperature, salinity and nitrogen (nitrate, ammonium, urea) uptake rates were measured. The concentration of phytoplankton is relatively high due to availability of high nutrients and low sea surface temperature. Phytoplankton community is dominated by diatoms whereas cryptophytes are in low concentration. The maximum concentration of total chlorophyll is 14.87 ${\mu}g\;L^{-1}$ and is attributed to upwelled subsurface winter water due to local wind forcing, availability of micro-nutrients and increased attenuation of photosynthetically available radiation (PAR). Concentration of blue-green algae is low compared to that of green algae because of low temperature. Comparatively high concentration of yellow substances is due to the influence of Antarctic melt-water whereas cryptophytes are low due to high salinity and mixed water column. Varied concentrations of phytoplankton at different times of Fluoroprobe measurements suggest that the coastal waters of Prydz Bay are influenced by changing sub-surface water temperature and salinity due to subsurface upwelling induced by local winds as also melting/freezing processes in late summer. The productivity is high in coastal water due to the input of macro as well as micro-nutrients.

Relative Microalgal Concentration in Prydz Bay, East Antarctica during Late Austral Summer, 2006

Rahul Mohan,Sunil Kumar Shukla,N. Anilkumar,M. Sudhakar,Satya Prakash,R. Ramesh 한국조류학회I 2009 ALGAE Vol.24 No.3

Microalgae using a submersible fluorescence probe in water column (up to 100 m) were measured during the austral summer of 2006 (February) in Prydz Bay, East Antarctica (triangular-shaped embayment in the Indian sector of Southern Ocean). Concurrently, environmental parameters such as temperature, salinity and nitrogen (nitrate, ammonium, urea) uptake rates were measured. The concentration of phytoplankton is relatively high due to availability of high nutrients and low sea surface temperature. Phytoplankton community is dominated by diatoms whereas cryptophytes are in low concentration. The maximum concentration of total chlorophyll is 14.87 μg L-1 and is attributed to upwelled subsurface winter water due to local wind forcing, availability of micro-nutrients and increased attenuation of photosynthetically available radiation (PAR). Concentration of blue-green algae is low compared to that of green algae because of low temperature. Comparatively high concentration of yellow substances is due to the influence of Antarctic melt-water whereas cryptophytes are low due to high salinity and mixed water column. Varied concentrations of phytoplankton at different times of Fluoroprobe measurements suggest that the coastal waters of Prydz Bay are influenced by changing sub-surface water temperature and salinity due to subsurface upwelling induced by local winds as also melting/freezing processes in late summer. The productivity is high in coastal water due to the input of macro as well as micro-nutrients.

Epidemic Propagation With Polarized Opinions Over Signed Network

Sourav Bhowmick,N. Mohan Kumar,Surajit Panja 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.11

This technical note deals with a coupled epidemic-opinion dynamical model over a multiplex network, where the interplay between polarized opinions over social interaction network regarding protective measures and disease spreading captured by susceptible-exposed-infected-vigilant (SEIV) epidemic model over transmission network is investigated. For this coupled model, sufficient condition of the disease free state is obtained for the network epidemic model, while the perceived disease severity drops to zero at this state through opinion sharing. The simulation results corroborate the findings.

Fabrication of Gas Metal Arc Welding Based Wire Plus Arc Additive Manufactured 347 Stainless Steel Structure: Behavioral Analysis Through Experimentation and Finite Element Method

R. Pramod,S. Mohan Kumar,A. Rajesh Kannan,N. Siva Shanmugam,Reza Tangestani 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.1

Wire plus arc additive manufacturing (WAAM) technology is utilized to fabricate a 347 stainless steel (SS347) plate usingthe gas metal arc welding process. The tensile properties of the WAAM plate revealed enhanced strength in comparisonwith the wrought alloy SS347. The microhardness and the ferrite measurement along the building direction (BD) were inthe range of 265–226 HV0.5 and 2.2–5.1 FN (Ferrite number), respectively. The microstructural features were comprehensively examined using electron backscatter difraction (EBSD) analysis and the Inverse pole fgure (IPF) maps revealed astrong <001> texture along the BD. Also, well-aligned equiaxed and columnar dendrites with a lower fraction of niobiumcarbide (NbC) was noticed in the microstructures of the as-built WAAM plate. A three-dimensional fnite element modelwas developed to simulate the build-up of a WAAM plate. Goldak heat source is used to model heat fux in thermal analysis,and the temperature distributions were predicted. Further, residual stress and plastic strain distributions were examined atvarious stages of the WAAM process.

Tunable UV-visible absorption of SnS2layered quantum dots produced by liquid phase exfoliation

Fu, Xiao,Ilanchezhiyan, P.,Mohan Kumar, G.,Cho, Hak Dong,Zhang, Lei,Chan, A. Sattar,Lee, Dong J.,Panin, Gennady N.,Kang, Tae Won The Royal Society of Chemistry 2017 Nanoscale Vol.9 No.5

<P>4H-SnS2 layered crystals synthesized by a hydrothermal method were used to obtain via liquid phase exfoliation quantum dots (QDs), consisting of a single layer (SLQDs) or multiple layers (MLQDs). Systematic downshift of the peaks in the Raman spectra of crystals with a decrease in size was observed. The bandgap of layered QDs, estimated by UV-visible absorption spectroscopy and the tunneling current measurements using graphene probes, increases from 2.25 eV to 3.50 eV with decreasing size. 2-4 nm SLQDs, which are transparent in the visible region, show selective absorption and photosensitivity at wavelengths in the ultraviolet region of the spectrum while larger MLQDs (5-90 nm) exhibit a broad band absorption in the visible spectral region and the photoresponse under white light. The results show that the layered quantum dots obtained by liquid phase exfoliation exhibit wellcontrolled and regulated bandgap absorption in a wide tunable wavelength range. These novel layered quantum dots prepared using an inexpensive method of exfoliation and deposition from solution onto various substrates at room temperature can be used to create highly efficient visible-blind ultraviolet photodetectors and multiple bandgap solar cells.</P>

Clinico-pathological Profile of Lung Cancer at AIIMS: A Changing Paradigm in India

Malik, Prabhat Singh,Sharma, Mehar Chand,Mohanti, Bidhu Kalyan,Shukla, N.K.,Deo, S.V.S.,Mohan, Anant,Kumar, Guresh,Raina, Vinod Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Background: Lung cancer is one of the commonest and most lethal cancers throughout the world. The epidemiological and pathological profile varies among different ethnicities and geographical regions. At present adenocarcinoma is the commonest histological subtype of non-small cell lung cancer (NSCLC) in most of the Western and Asian countries. However, in India squamous cell carcinoma has been reported as the commonest histological type in most of the series. The aim of the study was to analyze the current clinico-pathological profile and survival of lung cancer at our centre. Materials and Methods: We analyzed 434 pathologically confirmed lung cancer cases registered at our centre over a period of three years. They were evaluated for their clinical and pathological profiles, treatment received and outcome. The available histology slides were reviewed by an independent reviewer. Results: Median age was 55 years with a male:female ratio of 4.6:1. Some 68% of patients were smokers. There were 85.3% NSCLC and 14.7% SCLC cases. Among NSCLCs, adenocarcinoma was the commonest histological subtype after the pathology review. Among NSCLC, 56.8% cases were of stage IV while among SCLC 71.8% cases had extensive stage disease. Some 29% of patients did not receive any anticancer treatment. The median overall and progression free survivals of the patients who received treatment were 12.8 and 7.8 months for NSCLC and 9.1 and 6.8 months for SCLC. Conclusions: This analysis suggests that adenocarcinoma may now be the commonest histological subtype also in India, provided a careful pathological review is done. Most of the patients present at advanced stage and outcome remains poor.