Effect of Insecticide-Tolerant and Plant Growth-Promoting Mesorhizobium on the Performance of Chickpea Grown in Insecticide Stressed Alluvial Soils

Munees Ahemad,Mohammad Saghir Khan 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.4

An experiment was carried out to determine the plant growth-promoting activities of fipronil- and pyriproxyfen-tolerant Mesorhizobium isolates in the presence and absence of insecticides. The bioremediation and plant growth-promoting potentials of Mesorhizobium isolate (MRC4) was assessed using chickpea as a test crop grown under fipronil- and pyriproxyfen-stressed soils. In this study, the most promising mesorhizobial isolate (MRC4) tolerated fipronil and pyriproxyfen up to a concentration of 1600 μg ml-1 and 1400 μg ml-1, respectively. Isolate MRC4 produced a substantial amount of indole acetic acid (44.3 μg ml-1), salicylic acid (35 μg ml-1), 2,3 di-hydroxybenzoic acid (19 μg ml-1), and exo-polysaccharides (21 μg ml-1) in the absence of insecticides. The plant growth-promoting substances displayed by the isolate MRC4 declined progressively with increasing concentrations of each insecticide. The insecticide tolerant isolate MRC4 was further tested for its effect on chickpea plants grown in fipronil- and pyriproxyfentreated soils. The insecticide-tolerant isolate MRC4 increased the dry matter accumulation progressively. A maximum increase of 80 (at 600 μg kg-1 soil of fipronil) and 118% (at 3900 μg kg-1 soil of pyriproxyfen) was recorded 135 days after sowing when compared to non-inoculated plants treated with the same rates of each insecticide. Moreover, Mesorhizobium isolate MRC4 when used in fipronil- and pyriproxyfen-treated soil also increased symbiotic properties (nodulation and leghaemoglobin content), root N, shoot N, root P, shoot P, seed yield, and seed protein compared to the un-inoculated but treated solely with insecticide. The present finding suggests that the mesorhizobial isolate endowed with multiple properties could be used to facilitate the productivity of chickpea under insecticide-stressed soils. An experiment was carried out to determine the plant growth-promoting activities of fipronil- and pyriproxyfen-tolerant Mesorhizobium isolates in the presence and absence of insecticides. The bioremediation and plant growth-promoting potentials of Mesorhizobium isolate (MRC4) was assessed using chickpea as a test crop grown under fipronil- and pyriproxyfen-stressed soils. In this study, the most promising mesorhizobial isolate (MRC4) tolerated fipronil and pyriproxyfen up to a concentration of 1600 μg ml-1 and 1400 μg ml-1, respectively. Isolate MRC4 produced a substantial amount of indole acetic acid (44.3 μg ml-1), salicylic acid (35 μg ml-1), 2,3 di-hydroxybenzoic acid (19 μg ml-1), and exo-polysaccharides (21 μg ml-1) in the absence of insecticides. The plant growth-promoting substances displayed by the isolate MRC4 declined progressively with increasing concentrations of each insecticide. The insecticide tolerant isolate MRC4 was further tested for its effect on chickpea plants grown in fipronil- and pyriproxyfentreated soils. The insecticide-tolerant isolate MRC4 increased the dry matter accumulation progressively. A maximum increase of 80 (at 600 μg kg-1 soil of fipronil) and 118% (at 3900 μg kg-1 soil of pyriproxyfen) was recorded 135 days after sowing when compared to non-inoculated plants treated with the same rates of each insecticide. Moreover, Mesorhizobium isolate MRC4 when used in fipronil- and pyriproxyfen-treated soil also increased symbiotic properties (nodulation and leghaemoglobin content), root N, shoot N, root P, shoot P, seed yield, and seed protein compared to the un-inoculated but treated solely with insecticide. The present finding suggests that the mesorhizobial isolate endowed with multiple properties could be used to facilitate the productivity of chickpea under insecticide-stressed soils.

Effect of Insecticide-Tolerant and Plant Growth-Promoting Mesorhizobium on the Performance of Chickpea Grown in Insecticide Stressed Alluvial Soils

Ahemad, Munees,Khan, Mohammad Saghir 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.4

An experiment was carried out to determine the plant growth-promoting activities of fipronil- and pyriproxyfen-tolerant Mesorhizobium isolates in the presence and absence of insecticides. The bioremediation and plant growth-promoting potentials of Mesorhizobium isolate (MRC4) was assessed using chickpea as a test crop grown under fipronil- and pyriproxyfen-stressed soils. In this study, the most promising mesorhizobial isolate (MRC4) tolerated fipronil and pyriproxyfen up to a concentration of $1600\;{\mu}g\;ml^{-1}$ and $1400\;{\mu}g\;ml^{-1}$, respectively. Isolate MRC4 produced a substantial amount of indole acetic acid ($44.3\;{\mu}g\;ml^{-1}$), salicylic acid ($35\;{\mu}g\;ml^{-1}$), 2,3 di-hydroxybenzoic acid ($19\;{\mu}g\;ml^{-1}$), and exo-polysaccharides ($21\;{\mu}g\;ml^{-1}$) in the absence of insecticides. The plant growth-promoting substances displayed by the isolate MRC4 declined progressively with increasing concentrations of each insecticide. The insecticide tolerant isolate MRC4 was further tested for its effect on chickpea plants grown in fipronil- and pyriproxyfen-treated soils. The insecticide-tolerant isolate MRC4 increased the dry matter accumulation progressively. A maximum increase of 80 (at $600\;{\mu}g\;kg^{-1}$ soil of fipronil) and 118% (at $3900\;{\mu}g\;kg^{-1}$ soil of pyriproxyfen) was recorded 135 days after sowing when compared to non-inoculated plants treated with the same rates of each insecticide. Moreover, Mesorhizobium isolate MRC4 when used in fipronil- and pyriproxyfen-treated soil also increased symbiotic properties (nodulation and leghaemoglobin content), root N, shoot N, root P, shoot P, seed yield, and seed protein compared to the un-inoculated but treated solely with insecticide. The present finding suggests that the mesorhizobial isolate endowed with multiple properties could be used to facilitate the productivity of chickpea under insecticide-stressed soils.

정상 성인의 총 콜레스테롤/고밀도 콜레스테롤비와의 연관된 요인

문준식,오상우,전효이,조비룡,허봉렬 대한가정의학회 2000 Korean Journal of Family Medicine Vol.21 No.2

노년기 비만군과 정상 체중군 간의 우울 성향 비교

문준식,이용호,전세일,서호석,Mune, June-Sik,Lee, Yong-Ho,Chun, Sae-Il,Suh, Ho-Suk 대한불안의학회 2007 대한불안의학회지 Vol.3 No.2

Objective : Obesity has been considered to be associated with numerous physical, mental and psychological diseases. Depression, which is a major psychological factor affecting occurrence and treatment of obesity, can be a cause of obesity as well as can be triggered by obesity. This study aimed to find out the need of positive consideration to the existence of depressive mood in the management of obesity. Methods : A total of 101 subjects were enrolled among those who were 60 years old and over at one local clinic. Obese group (n=49) was defined as BMI${\geqq}$25 and normal weight group (n=52) as 20<BMI<23. Their depressive disposition was evaluated by Geriatric Depression Scale Short Form Korea Version (GDSSFK). Those who had previous history of psychological diseases like depression or are suffering from severe medical diseases were excluded. Results : The mean GDSSF-K score of the obese group ($5.2{\pm}2.5$) was significantly higher than that of the normal weight group ($3.8{\pm}1.7$)(p<0.05). In male subjects, there was no significant difference in the mean GDSSF-K score between both groups, but in female subjects, the mean GDSSF-K score of the obese group, $6.4{\pm}2.6$ was significantly higher than that of the normal weight group ($4.0{\pm}1.8$)(p<0.05). Conclusion : The elderly obese group showed higher depressive scale score than elderly normal weight group, especially in female elderly. Since the elderly obese female group had a depressive predisposition, comprehensive management including mental and psychological approach is required in obesity control programs.