Synthesis and anti-mycobacterial activity of 4-(4-phenyl-1H-1,2,3- triazol-1-yl)salicylhydrazones: revitalizing an old drug

Hajjaj H. M. Abdu-Allah,Bahaa G. M. Youssif,Mostafa H. Abdelrahman,Mohammed K. Abdel-Hamid,Rudraraju Srilakshmi Reshma,Perumal Yogeeswari,Tarek Aboul-Fadl,Dharmarajan Sriram 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.2

The antitubercular drug; para-aminosalicylicacid (PAS) was used as the core scaffold for the design of aseries of 1H-1,2,3-triazolylsalicylhydrazones upon couplingwith triazole and arylhydrazone moietis to furnish asingle molecular architecture. The obtained derivativeswere screened against Mycobacterium tuberculosis H37Rvrevealing good to high activity for the active compounds(MIC values of 0.39–1.5 lg/mL) compared to the marketeddrugs isoniazid, rifampicin and ethambutol. Moreover, themost active analogue N-(1-(4-chlorobenzyl)-2-oxoindolin-3-ylidene)-2-hydroxy-4-(4-phenyl-1H-1,2,3-triazol-1-yl)-benzohydrazide (20) was found to be ten-fold more potentthan PAS and equipotent to rifampicin (MIC 0.39 lg/mL),while exhibiting low cytotoxicity with a selectivity indexof[128. In addition, this compound was shown to beactive against persistent forms of mycobacteria comparableto standard drugs in nutrient starvation model. Accordingly,we introduce compound 20 as a valuable lead forfurther development. A 3D-QSAR study was also conductedto help in explaining the observed activity and toserve as a tool for further development.

Plasmonic-Multichromatic Sensor for Gallic Acid Assay Utilizing Ag-Coated Au Nanobipyramids

Mostafa Mohamed H.,Shaban Samy M.,Hafez Eslam,Shin Jihoon,김동환 한국바이오칩학회 2023 BioChip Journal Vol.17 No.4

Gallic acid (GA) is known for its valuable properties as an antioxidant, anti-cancer, and anti-mutagenic compound, making its detection in foods and drugs of paramount importance. In this research, we introduce a novel multichromatic sensor for GA detection, which employs the controlled growth of silver nanoparticles (AgNPs) on the surface of gold nanobipyramids (AuNBPs). The sensor exploits GA's capability to reduce AgNO 3, resulting in the growth of AgNPs and a subsequent blue shift of localized surface plasmon resonance (LSPR) as large as 195 nm. This unique multicolor response ranges from light gray to green, blue–violet, and pink, allowing for distinctive visual identifi cation of GA concentrations. The sensor's performance demonstrates a wide dynamic range of 0–175 μM, and a detection limit (LOD) as low as 0.139 μM. Notably, the applicability of this multichromic plasmonic probe was successfully tested for GA assay in both Black and Green tea, showcasing highly satisfactory recovery effi ciencies and affi rming its potential for food quality control applications. The presented multichromatic sensor off ers a promising approach for rapid and sensitive GA detection in various food and pharmaceutical products, enhancing the monitoring and assessment of GA content for improved product quality and safety.

Ameliorative Effects of Pomegranate Peel Extract against Dietary-Induced Nonalcoholic Fatty Liver in Rats

Siham N. K. Al-Shaaibi,Mostafa I. Waly,Lyutha Al-Subhi,Mohamed H. Tageldin,Nada M. Al-Balushi,Mohammad S. Rahman 한국식품영양과학회 2016 Preventive Nutrition and Food Science Vol.21 No.1

Non-alcoholic fatty liver disease (NAFLD) is caused by fat accumulation and is associated with oxidative stress. In this study, we investigated the potential protective effect of pomegranate (Punica granatum L.) peel extract (PPE) against oxidative stress in the liver of rats with NAFLD. Sprague-Dawley rats were fed a high fat diet (HFD), 20% corn oil, or palm oil for 8 weeks in the presence or absence of PPE. The control group was fed a basal diet. The progression of NAFLD was evaluated histologically and by measuring liver enzymes (alanine transaminase and aspartate transaminase), serum lipids (triglycerides and total cholesterol), and oxidative stress markers. The HFD feeding increased the body weight and caused NAFLD, liver steatosis, hyperlipidemia, oxidative stress, and elevated liver enzymes. Administration of PPE ameliorated the hepatic morphology, reduced body weight, improved liver enzymes, and inhibited lipogenesis. Furthermore, PPE enhanced the cellular redox status in the liver tissue of rats with NAFLD. Our findings suggest that PPE could improve HFD-induced NAFLD via abolishment of hepatic oxidative damage and hyperlipidemia. PPE might be considered as a potential lead material in the treatment of NAFLD and obesity through the modulation of lipid metabolism.

Ameliorative Effects of Pomegranate Peel Extract against Dietary-Induced Nonalcoholic Fatty Liver in Rats

Al-Shaaibi, Siham N.K.,Waly, Mostafa I.,Al-Subhi, Lyutha,Tageldin, Mohamed H.,Al-Balushi, Nada M.,Rahman, Mohammad Shafiur The Korean Society of Food Science and Nutrition 2016 Preventive Nutrition and Food Science Vol.21 No.1

Non-alcoholic fatty liver disease (NAFLD) is caused by fat accumulation and is associated with oxidative stress. In this study, we investigated the potential protective effect of pomegranate (Punica granatum L.) peel extract (PPE) against oxidative stress in the liver of rats with NAFLD. Sprague-Dawley rats were fed a high fat diet (HFD), 20% corn oil, or palm oil for 8 weeks in the presence or absence of PPE. The control group was fed a basal diet. The progression of NAFLD was evaluated histologically and by measuring liver enzymes (alanine transaminase and aspartate transaminase), serum lipids (triglycerides and total cholesterol), and oxidative stress markers. The HFD feeding increased the body weight and caused NAFLD, liver steatosis, hyperlipidemia, oxidative stress, and elevated liver enzymes. Administration of PPE ameliorated the hepatic morphology, reduced body weight, improved liver enzymes, and inhibited lipogenesis. Furthermore, PPE enhanced the cellular redox status in the liver tissue of rats with NAFLD. Our findings suggest that PPE could improve HFD-induced NAFLD via abolishment of hepatic oxidative damage and hyperlipidemia. PPE might be considered as a potential lead material in the treatment of NAFLD and obesity through the modulation of lipid metabolism.

Nonlocal strain gradient theory for buckling and bending of FG-GRNC laminated sandwich plates

Muhammad Basha,Ahmed Amine Daikh,Ammar Melaibari,Ahmed Wagih,Ramzi Othman,Khalid H. Almitani,Mostafa A. Hamed,Alaa Abdelrahman,Mohamed A Eltaher 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.43 No.5

The bending and buckling behaviours of FG-GRNC laminated sandwich plates are investigated by using novel fivevariables quasi 3D higher order shear deformation plate theory by considering the modified continuum nonlocal strain gradient theory. To calculate the effective Young’s modulus of the GRNC sandwich plate along the thickness direction, and Poisson’s ratio and mass density, the modified Halpin-Tsai model and the rule of the mixture are employed. Based on a new field of displacement, governing equilibrium equations of the GRNC sandwich plate are solved using a developed approach of Galerkin method. A detailed parametric analysis is carried out to highlight the influences of length scale and material scale parameters, GPLs distribution pattern, the weight fraction of GPLs, geometry and size of GPLs, the geometry of the sandwich plate and the total number of layers on the stresses, deformation and critical buckling loads. Some details are studied exclusively for the first time, such as stresses and the nonlocality effect.

New Haplotypes of the ATP Synthase Subunit 6 Gene of Mitochondrial DNA are Associated with Acute Lymphoblastic Leukemia in Saudi Arabia

Yacoub, Haitham Ahmed,Mahmoud, Wael Mahmoud,El-Baz, Hatim Alaa-Eldeen El-Din,Eid, Ola Mohamed,El-Fayoumi, Refaat Ibrahim,Mahmoud, Maged Mostafa,Harakeh, Steve,Abuzinadah, Osama H.A. Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: Acute lymphoblastic leukemia (ALL) is the most common cancer diagnosed in children and represents approximately 25% of cancer diagnoses among those younger than 15 years of age. Aim and Objectives: This study investigated substitutions in the ATP synthase subunit 6 gene of mitochondrial DNA (mtDNA) as a potential diagnostic biomarker for early detection and diagnosis of acute lymphoblastic leukemia. Based on mtDNA from 23 subjects diagnosed with acute lymphoblastic leukemia, approximately 465 bp of the ATP synthase subunit 6 gene were amplified and sequenced. Results: The sequencing revealed thirty-one mutations at 14 locations in ATP synthase subunit 6 of mtDNA in the ALL subjects. All were identified as single nucleotide polymorphisms (SNPs) with a homoplasmic pattern. The mutations were distributed between males and females. Novel haplotypes were identified in this investigation: haplotype (G) was recorded in 34% in diagnosed subjects; the second haplotype was (C) with frequency of 13% in ALL subjects. Neither of these were observed in control samples. Conclusions: These haplotypes were identified for the first time in acute lymphoblastic leukemia patients. Five mutations able to change amino acid synthesis for the ATP synthase subunit 6 were associated with acute lymphoblastic leukemia. This investigation could be used to provide an overview of incidence frequency of acute lyphoblastic leukemia (ALL) in Saudi patients based on molecular events.