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A NOTE ON DEGREES OF TWISTED ALEXANDER POLYNOMIALS II
Morifuji, Takayuki,Suzuki, Masaaki Korean Mathematical Society 2013 대한수학회보 Vol.50 No.3
In this short note we discuss degrees of twisted Alexander polynomials and demonstrate an explicit example of a closed 3-manifold which is related to the degree formula due to Friedl, Kim and Kitayama.
Representations of the Braid Group and Punctured Torus Bundles
Morifuji, Takayuki,Suzuki, Masaaki Department of Mathematics 2009 Kyungpook mathematical journal Vol.49 No.1
In this short note, we consider a family of linear representations of the braid group and the fundamental group of a punctured torus bundle over the circle. We construct an irreducible (special) unitary representation of the fundamental group of a closed 3-manifold obtained by the Dehn filling.
A note on degrees of twisted Alexander polynomials II
Takayuki Morifuji,Masaaki Suzuki 대한수학회 2013 대한수학회보 Vol.50 No.3
In this short note we discuss degrees of twisted Alexander polynomials and demonstrate an explicit example of a closed 3-manifold which is related to the degree formula due to Friedl, Kim and Kitayama.
( Yohei Morifuji ),( Kenji Kubota ),( Shiro Tanaka ),( Akira Jomori ),( Atsuyoshi Jomori ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2
Slope failures due to heavy rain and earthquakes have occurred frequently in Japan. To evaluate the risk of slope failures, it is necessary to survey the subsurface structure and identify areas having risk of collapses. However, much time and labor is associated with conducting surveys on slopes, and the range of the survey area is often limited. Therefore, in this study, the grounded electrical source airborne transient electromagnetic system using a drone (D-GREATEM) was applied to a slope to reveal the resistivity structure which is an index of the rock geological features and the groundwater level. To avoid fall accidents, the drone flied at several constant elevations. Although the distance between the platform and the ground should be constant for analysis, it is difficult to maintain a specific flight height from the ground on a slope. This problem was approached by correcting the flight height in the analysis. To evaluate the resistivity results, a ground electrical survey was also conducted. The resistivity structure obtained from the electromagnetic survey conducted using the drone showed three layers of resistivity: a higher resistivity zone at depths shallower than 30 m, a lower resistivity zone from 50-100 m, and a higher resistivity zone at greater depth. The shallowest higher resistivity zone indicates the detritus and talus deposit distributed near the surface. The ground electrical survey also showed a higher resistivity zone in the area. The electromagnetic survey conducted using the drone could easily obtain the resistivity structure in the slope.
Takumi Yano,이재만,Jian Xu,Yoshiki Morifuji,Akitsu Masuda,Masato Hino,Daisuke Morokuma,Ryosuke Fujita,Masateru Takahashi,Takahiro Kusakabe,Hiroaki Mon 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.2
Reverse transcriptase from Moloney murine leukemia virus (MMLVRT) is an RNA dependent DNA polymerase, which has been used as a fundamental tool for molecular biology and biotechnology. The secondary structures formed in the RNA templates decrease the accessibility of the reverse transcriptase to the RNA templates; it is important to unfold the RNA secondary structure by increasing the reaction temperature to perform the successful transcription. In this study, we applied silkworm baculovirus expression vector system (silkworm-BEVS) to mass-produce and purify the recombinant MMLVRTs with the N- or C- terminal tandem tags. We confirmed that both of the recombinant MMLVRT enzymes have intact DNA polymerase activity. It is notable that Cterminal tagged MMLVRT outperformed MMLVRT obtained from the E. coli expression system in terms of thermostability and sensitivity to low quantities of RNA template. Taken together, these results demonstrate that silkworm-BEVS is a promising alternative strategy to produce the functional and thermostable reverse transcriptase.
Akitsu Masuda,Jian Xu,Kosuke Minamihata,Genki Kagawa,Yusei Hamada,Yoshiki Morifuji,Takumi Yano,Masato Hino,Daisuke Morokuma,Noriko Karasaki,Hiroaki Mon,Noriho Kamiya,Takahiro Kusakabe,이재만 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2
As a therapeutic treatment, recombinant human basic fibroblast growth factor (rhbFGF) is usually employed in tissue regeneration, and as an essential component in culture medium for maintaining the induced pluripotent stem (iPS) cell and embryonic stem (ES) cell in an undifferentiated state. Therefore, a large amount of biologically active rhbFGF is required. In this study, silkworm-baculovirus expression vector system (silkworm-BEVS) is employed to achieve a high productivity of recombinant rhbFGF with two small affinity tags (His-tag and STREP-tag) at the N or C-terminus. It is observed that rhbFGF with 30 K signal peptide of silkworm were successfully expressed but are not sufficiently secreted into the culture medium of cultured insect cells. Then we purified the N- or C-tagged intracellular rhbFGF protein and obtained a yield of about 0.7 mg/larva and 1.2 mg/ larva, respectively. Although the final yield of the C-tagged rhbFGF is higher than that of the N-tagged, rhbFGF with N-tag demonstrated promising and comparable biological activity, which is evaluated through a mammalian cell proliferation assay. Taken together, these results indicate that silkworm-BEVS could contribute to the mass-production of the biologically active rhbFGF for medical uses.