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Iman Roohollahi,Mohsen Kafi 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.2
Plant growth regulators are frequently applied for warm-season turf just before over-seeding to increase the turf performance, and its competitive ability. In situation that the turfs are irrigated with saline water, the time of TE application on turf is critical. Seeds of two perennial ryegrass cultivars were adjusted to different salinity levels (0, 2000, 4000 and 6000 ㎎?L<SUP>-1</SUP> NaCl) and TE (0, 50 and 100 g?㏊<SUP>-1</SUP>) levels which individually performed in agar and soil to detect their effects on germination and initial growth. Seed germination percentage, germination rate, weight of mobilized seed reserve and seed reserve depletion percentage were significantly affected by TE, and salinity with an exception of seed reserve utilization efficiency in the first experiment. TE inhibited germination percentage more severely in higher concentration. The results showed that TE had no significant effect on chlorophyll concentration, height, electrolyte leakage, root length, the fresh and dry weight of seedling, but salinity significantly decreased all of them. It postulated that TE application before over-seeding had deleterious effects on over-seeded grass germination; however, because it had no effects on initial growth of turf, it can be used in this stage.
Evaluation of Genetic Diversity among Some Genotypes of Kentucky Bluegrass by RAPD Molecular Markers
Javad Rezapour Fard,Zabihollah Zamani,Mohamad Reza Fattahi Moghaddam,Mohsen Kafi 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.4
Genetic improvement of plants need a high genetic diversity and molecular markers offer a powerful tool for its assessment. Poa pratensis has extensive divergence in natural old turfs and pastures in temperate zones of Iran,being useful for breeding programs. In this research, RAPD markers were used to study the genetic diversity of 19turfgrass genotypes (including 16 genotypes of Poa pratensis along with one genotype from each of Lolium perenne,Cynodon dactylon and Festuca arundinacea species). After screening 100 random 10-mer RAPD primers, 17 primers were selected based on the clarity of their amplified fragments in PCR and the high reproducibility for scoring. From the 284 produced bands, 281 were polymorphic. Cluster analysis of RAPD data was performed using the UPGMA method based on Jaccard's similarity coefficients. Dendrogram at a similarity of 0.24 gave 5 main clusters. The correlation coefficient between the data matrix and the cophenetic matrix of cluster data was high (r = 0.99), indicating that the clustering dendrogram being highly fitted to similarity matrix. A relatively high genetic similarity was observed between some commercial cultivars and Iranian local genotypes. In addition, this research showed the high genetic diversity among studied genotypes as well as the high efficiency of RAPD markers for evaluation of genetic diversity among turfgrasses.