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Influence of Apical Meristem Culture and Thermotherapy on Production of Healthy Fig Plants
Amir Sahraroo,Mesbah Babalar,Ali Ebadi,Mina Kouhi Habibi,Abdollah Khadivi-Khub 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.1
In this study, production of healthy fig plant using apical meristem culture and thermotherapy in fig (Ficus carica L. cv. Jaami-e-Kan) was conducted. At first, apical meristem (0.2-0.4 and 0.5-0.7 ㎜ in size) of the plant with evident fig mosaic disease (FMDs) symptoms were developed on MS solid medium supplemented with 0.5 ㎎ㆍℓ<SUP>-1</SUP> 6-benzyladenine (BA) and 0.1 ㎎ㆍℓ<SUP>-1</SUP> α-naphthalene acetic acid (NAA). In addition, nodal segment from meristemic microshoots were proliferated on MS medium supplemented with BA (1, 1.5 and 2 ㎎ㆍℓ<SUP>-1</SUP>) and NAA (0, 0.1 and 0.5 ㎎ㆍℓ<SUP>-1</SUP>). Finally, the plantlets were rooted on half strength MS medium containing IBA (0.5, 1, 1.5 and 2 ㎎ㆍℓ<SUP>-1</SUP>). Significant differences were observed between the treatments. Smaller meristems (0.2-0.4 ㎜) showed the best result in removing the FMDs symptoms after 12 months (93%). Shoot proliferation rate was found to be higher in the medium containing 2 ㎎ㆍℓ<SUP>-1</SUP> BA than other concentrations. Highest numbers of rooted plantlets were obtained from 1.5 and 2 ㎎ㆍℓ<SUP>-1</SUP> IBA. At the second experiment, potted plantlets with evident symptoms were subjected with two alternating high temperature regime (35/40℃ and 30/35℃ dark/light) and were evaluated after 4 and 6 weeks. Thermotherapy regime with 35/40℃ dark/light during 6 weeks was significantly (p<0.05) effective on removing the FMDs symptoms (98%).
Abdolkarim Zarei,Amir Sahraroo 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.2
Pomegranate is a long-cultivated fruit tree believed to have originated in Iran. In the present study, 16 preselected nuclear microsatellite markers, or simple sequence repeats (SSRs), were analyzed in 50 pomegranate (Punica granatum L.) accessions from five regions in Fars province of Iran. Each SSR loci was polymorphic and produced 48 fragments in the studied samples. The mean expected and observed heterozygosity of the 16 SSR loci was 0.33 and 0.48, respectively. The polymorphic information content ranged from 0.18 to 0.58 with an average of 0.41. There were some differences regarding diversity indices among populations, and several private alleles were detected in different populations, indicating the importance of these accessions for genetic conservation. Cluster analysis using SSR data grouped genotypes largely based on their geographical origins. Analysis of molecular variance showed that most of the genetic variation was among populations. Genetic synonymy was observed in some pomegranate accessions located across geographical regions. A relatively high level of genetic admixture was found among accessions from different regions, suggesting that there is a high level of genetic exchange between individual genotypes. This work assesses the genetic diversity and population structure of pomegranates in Fars province, which assists in future conservation and breeding programs.