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- 저자 : Mohamed Shafi (검색결과 24 건)
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Seismic pounding effects on adjacent buildings in series with different alignment configurations
Shehata E. Abdel Raheem,Mohamed Y.M. Fooly,Aly G.A. Abdel Shafy,Yousef A. Abbas,Mohamed Omar,Mohamed M.S. Abdel Latif,Sayed Mahmoud 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.28 No.3
Numerous urban seismic vulnerability studies have recognized pounding as one of the main risks due to the restricted separation distance between neighboring structures. The pounding effects on the adjacent buildings could extend from slight non-structural to serious structural damage that could even head to a total collapse of buildings. Therefore, an assessment of the seismic pounding hazard to the adjacent buildings is superficial in future building code calibrations. Thus, this study targets are to draw useful recommendations and set up guidelines for potential pounding damage evaluation for code calibration through a numerical simulation approach for the evaluation of the pounding risks on adjacent buildings. A numerical simulation is formulated to estimate the seismic pounding effects on the seismic response demands of adjacent buildings for different design parameters that include: number of stories, separation distances; alignment configurations, and then compared with nominal model without pounding. Based on the obtained results, it has been concluded that the severity of the pounding effects depends on the dynamic characteristics of the adjacent buildings and the input excitation characteristics, and whether the building is exposed to one or two-sided impacts. Seismic pounding among adjacent buildings produces greater acceleration and shear force response demands at different story levels compared to the no pounding case response demands.
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Mode of Organogenesis of Epiphyllous Bud in Kalanchoe daigremontiana During In Vitro Culture System
Mohammed Shafi Ullah Bhuiyan,Kim, Tehryung,Choi, Kwan Sam 충남대학교 생물공학연구소 2005 생물공학연구지 Vol.11 No.2
Extensive segmentation of detached leaves bearing variable number of dormant buds has been done to investigate the mode of organogenesis of epiphyllous buds on the leaves in Kalanchoe daigremontiana. The study revealed that shoot always emerged prior to root emergence from each bud. The buds from the middle region of the leaves were more responsive (earlier organogenesis) and more uniformly so than those from the distal regions. The existence of such a pattern of organogenesis even in leaf cuttings and squares points to the operation of some endogenous physiological gradients along linear leaf axis, not disturbed by system miniaturization. Enlargement of photosynthetic tissue at the disposal of a single bud did not accelerate organogenesis.
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Mohammed Shafi Ullah Bhuiyan,임용표,민성란,최관삼,유장렬 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.1
An efficient and reproducible plant regeneration system was established using transverse thin cell layers (tTCLs) in five cultivars of Brassica juncea L. The effects of medium conditions, explant types (tTCLs of hypcotyl and cotyledonary petiole) on shoot regeneration were examined in this study. The maximum shoot regeneration frequency was obtained in Murashige and Skoog (MS) medium supplemented with 4 mg/L 6-benzylaminopurine (BA) and 0.2 mg/L 1-naphthaleneacetic acid (NAA). The hypocotyls derived tTCL explants had more shoot regeneration frequency (52%) than the cotyledonary petiole derived tTCL explants. Shoot induction was further improved by the addition of silver nitrate (AgNO3) in the regeneration medium. A significant genotypic effect was also observed between the five cultivars; Rai-5 displayed higher capacities to produce shoots than other cultivars. Regenerated shoots were rooted on MS basal medium without PGRs which induced 90% of roots. The plantlets established in greenhouse conditions with 99% survival, flowered normally and set seeds. The regenerated plants were fertile and identical to source plants.
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Mohammed Shafi Ullah Bhuiyan,민성란,최관삼,임용표,유장렬 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.2
An efficient system for high frequency plant regeneration was established through investigating various factors such as plant growth regulator combinations, explant types and ages, and addition of AgNO3 influenced on shoot regeneration in Brassica juncea L. cv. BARI sarisha-10. Murashige and Skoog (MS) medium supplemented with 0.1 mg/L NAA (1-naphthaleneacetic acid) and 1 mg/L BA (6-benzyladenine) showed the maximum shoot regeneration frequency (56.67%) among the different combinations of NAA and BA. Explant type, explant age, and addition of AgNO3 also significantly affected shoot regeneration. Of the four type of explants (cotyledon, hypocotyl, root, and leaf explants)- cotyledon explants produced the highest shoot regeneration frequency and hypocotyls explants produced the highest number of shoots per explant, whereas root explants did not produce any shoot. The cotyledonary explants from Four-day-old seedlings showed the maximum shoot regeneration frequency and number of shoots per explant. Shoot regeneration frequency increased significantly by adding AgNO3 to the medium. Two mg/L AgNO3 appeared to be the best for shoot regeneration with the highest shoot regeneration frequency (86.67%) and number of shoots per explant (7.5 shoots). Considerable variation in shoot regeneration from cotyledonay explants was observed within the B. juncea L. genotypes. The shoot regeneration frequency ranged from 47.78% for cv. Shambol to 91.11% for cv. Rai-5. In terms of the number of shoots produced per explant, B. juncea L. cv. Daulot showed the maximum efficiency. MS medium supplemented with 0.1 mg/L NAA showed the highest frequency of rooting. The regenerated plantlets were transferred to pot soil and grown to maturity in the greenhouse. All plants were fertile and morphologically identical with the source plants.
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( Mohamed Azab ),( Loomee Doo ),( Daniel H. Doo ),( Yousif Elmofti ),( Muazer Ahmed ),( John Jay Cadavona ),( Xibei B. Liu ),( Amaan Shafi ),( Moon Kyung Joo ),( Ji Won Yoo ) 대한간학회 2017 Gut and Liver Vol.11 No.6
Background/Aims: Although proton pump inhibitors (PPIs) have been widely used for the prevention and treatment of stress gastric ulcers in hospital settings, there are concerns that PPIs increase the risk of Clostridium difficile infection (CDI). However, little is known about the risk of CDI follow ing PPI and histamine-2 receptor antagonist (H2RA) use. We evaluated the comparative hospital-acquired CDI occur rence risk associated with the concurrent use of PPIs versus H2RAs. Methods: A systematic search of PubMed, MED LINE/Ovid, Cumulative Index to Nursing and Allied Health Literature (CINAHL), Web of Science, and Google Scholar through August 19, 2016, identified 12 studies that reported the hospital-acquired CDI occurrence following H2RA and PPI use for the prevention and treatment of stress gastric ulcers. Random-effects pooled odds ratios and 95% confidence intervals were estimated. Heterogeneity was measured using I2, and a meta-regression analysis was conducted. The Grad ing of Recommendations Assessment, Development, and Evaluation (GRADE) system was used to assess the overall quality of the evidence. Results: A total of 74,132 patients from 12 observational studies were analyzed. Compared to H2RAs, PPIs increased the risk of CDI by 38.6% (pooled odds ratio, 1.386; 95% confidence interval, 1.152 to 1.668; p=0.001; I2=42.81%). Subgroup analyses of the purpose of study medication use, study site, and study design con firmed the consistency of a greater CDI risk with PPIs than with H2RAs. The overall quality of evidence was rated as low. Conclusions: The use of PPIs for both the prevention and treatment of stress ulcers was associated with a 38.6% in creased risk of hospital-acquired CDI occurrence compared to H2RA use. (Gut Liver 2017;11:781-788)
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Alhazmi, Mohammed I.,Hasan, Tarique N.,Shafi, Gowhar,Al-Assaf, Abdullah H.,Alfawaz, Mohammed A.,Alshatwi, Ali A. Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22
Background: Nigella Sativa (NS) is an herb from the Ranunculaceae family that exhibits numerous medicinal properties and has been used as important constituent of many complementary and alternative medicines (CAMs). The ability of NS to kill cancer cells such as PC3, HeLa and hepatoma cells is well established. However, our understanding of the mode of death caused by NS remains nebulous. The objective of this study was to gain further insight into the mode and mechanism of death caused by NS in breast cancer MCF-7 cells. Materials and Methods: Human breast cancer cells (MCF-7) were treated with a methanolic extract of NS, and a dose- and time-dependent study was performed. The $IC_{50}$ was calculated using a Cell Titer $Blue^{(R)}$ viability assay assay, and evidence for DNA fragmentation was obtained by fluorescence microscopy TUNEL assay. Gene expression was also profiled for a number of apoptosis-related genes (Caspase-3, -8, -9 and p53 genes) through qPCR. Results: The $IC_{50}$ of MCF-7 cells was $62.8{\mu}L/mL$. When MCF-7 cells were exposed to $50{\mu}L/mL$ and $100{\mu}L/mL$ NS for 24h, 48h and 72h, microscopic examination (TUNEL assay) revealed a dose- and time-dependent increase in apoptosis. Similarly, the expression of the Caspase-3, -8, -9 and p53 genes increased significantly according to the dose and time. Conclusions: NS induced apoptosis in MCF-7 cells through both the p53 and caspase pathways. NS could potentially represent an alternative source of medicine for breast cancer therapy.
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Bhuiyan, Mohammed Shafi Ullah,Min, Sung Ran,Jeong, Won Joong,Sultana, Sayeda,Choi, Kwan Sam,Lim, Yong Pyo,Song, Won Yong,Lee, Youngsook,Liu, Jang R. Japanese Society for Plant Cell and Molecular Biol 2011 Plant biotechnology Vol.28 No.1
<P>An efficient <I>Agrobacterium</I>-mediated genetic transformation method was established for <I>Brassica juncea</I> by investigating several factors responsible for successful gene transfer. Four-day-old cotyledon explants from <I>in vitro</I> grown seedlings were co-cultivated with <I>Agrobacterium</I> strain GV3101 harboring the binary vector EnPCAMBIA1302-YCF1, which contained the hygromycin phosphotransferase (<I>HPT</I>) gene as a selectable marker and the yeast cadmium factor 1 (<I>YCF1</I>) gene. Two days co-cultivation period on shoot induction medium (MS medium supplemented with 0.1 mg l<SUP>−1</SUP> α-naphthaleneacetic acid, 1.0 mg l<SUP>−1</SUP> 6-benzyladenine, and 2.0 mg l<SUP>−1</SUP> silver nitrate) containing 20 mg l<SUP>−1</SUP> acetosyringone and five days delaying exposure of explants to selective agent enhanced transformation efficiency significantly. A three-step selection strategy was developed to select hygromycin resistant shoots. Hygromycin-resistant shoots were subsequently rooted on root induction medium. Rooted plantlets were transferred to pot-soil, hardened, and grown in a greenhouse until maturity. Using the optimized transformation procedure, transformation efficiency reached at 16.2% in this study. Southern blot analysis was performed to confirm that transgenes (<I>HPT</I> and <I>YCF1</I>) were stably integrated into the plant genome. All transgenic plants showed single-copy of transgene integration in the host genome. Segregation analysis of T<SUB>1</SUB> progeny showed that the transgenes were stably integrated and transmitted to the progeny in a Mendelian fashion.</P>
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Bhuiyan, Mohammed Shafi Ullah,Min, Sung-Ran,Choi, Kwan-Sam,Lim, Yong-Pyo,Liu, Jang-Ryol The Korean Society of Plant Biotechnology 2009 식물생명공학회지 Vol.36 No.2
An efficient system for high frequency plant regeneration was established through investigating various factors such as plant growth regulator combinations, explant types and ages, and addition of $AgNO_3$ influenced on shoot regeneration in Brassica juncea L. cv. BARI sarisha-10. Murashige and Skoog (MS) medium supplemented with 0.1 mg/L NAA (1-naphthaleneacetic acid) and 1 mg/L BA (6-benzyladenine) showed the maximum shoot regeneration frequency (56.67%) among the different combinations of NAA and BA. Explant type, explant age, and addition of $AgNO_3$ also significantly affected shoot regeneration. Of the four type of explants (cotyledon, hypocotyl, root, and leaf explants)- cotyledon explants produced the highest shoot regeneration frequency and hypocotyls explants produced the highest number of shoots per explant, whereas root explants did not produce any shoot. The cotyledonary explants from Four-day-old seedlings showed the maximum shoot regeneration frequency and number of shoots per explant. Shoot regeneration frequency increased significantly by adding $AgNO_3$ to the medium. Two mg/L $AgNO_3$ appeared to be the best for shoot regeneration with the highest shoot regeneration frequency (86.67%) and number of shoots per explant (7.5 shoots). Considerable variation in shoot regeneration from cotyledonay explants was observed within the B. juncea L. genotypes. The shoot regeneration frequency ranged from 47.78% for cv. Shambol to 91.11% for cv. Rai-5. In terms of the number of shoots produced per explant, B. juncea L. cv. Daulot showed the maximum efficiency. MS medium supplemented with 0.1 mg/L NAA showed the highest frequency of rooting. The regenerated plantlets were transferred to pot soil and grown to maturity in the greenhouse. All plants were fertile and morphologically identical with the source plants.
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