apoptosis in N-acetyl-p-aminophenol-induced liver injury in mice is tissue factor dependent

Mohamed Sadek Abdel-Bakky,Gouda Kamel Helal,El-Sayed Mohamed El-Sayed,Elham Amin,Abdulmajeed Alqasoumi,Ahmad Alhowail,Eman Sayed Said Abdelmoti,Ahmed Saad Saad 대한생리학회-대한약리학회 2021 The Korean Journal of Physiology & Pharmacology Vol.25 No.5

Tissue factor (TF) activates the coagulation system and has an important role in the pathogenesis of various diseases. Our previous study stated that retinoid receptors (RAR-α and RXR-α) are released as a lipid droplet in monocrotaline/ lipopolysaccharide-induced idiosyncratic liver toxicity in mice. Herein, the interdependence between the release of retinoid receptors RAR-α and RXR-α and TF in Nacetyl- p-aminophenol (APAP)-induced mice liver toxicity, is investigated. Serum alanine transaminase (ALT) level, platelet and white blood cells (WBCs) counts, protein expression of fibrin, TF, cyclin D1 and cleaved caspase-3 in liver tissues are analyzed. In addition, histopathological evaluation and survival study are also performed. The results indicate that using of TF-antisense (TF-AS) deoxyoligonucleotide (ODN) injection (6 mg/kg), to block TF protein synthesis, significantly restores the elevated level of ALT and WBCs and corrects thrombocytopenia in mice injected with APAP. TF-AS prevents the peri-central overexpression of liver TF, fibrin, cyclin D1 and cleaved caspase- 3. The release of RXR-α and RAR-α droplets, in APAP treated sections, is inhibited upon treatment with TF-AS. In conclusion, the above findings designate that the released RXR-α and RAR-α in APAP liver toxicity is TF dependent. Additionally, the enhancement of cyclin D1 to caspase-3-dependent apoptosis can be prevented by blocking of TF protein synthesis.

Does intrauterine injection of low-molecular-weight heparin improve the clinical pregnancy rate in intracytoplasmic sperm injection?

Kamel, Ahmed Mohamed,El-Faissal, Yahia,Aboulghar, Mona,Mansour, Ragaa,Serour, Gamal I,Aboulghar, Mohamed The Korean Society for Reproductive Medicine 2016 Clinical and Experimental Reproductive Medicine Vol.43 No.4

Objective: Heparin can modulate proteins, and influence processes involved in implantation and trophoblastic development. This study aimed to assess the improvement of clinical pregnancy and implantation rates after local intrauterine injection of low-molecular-weight heparin (LMWH) in patients undergoing intracytoplasmic sperm injection (ICSI). Methods: A randomised case/control design was followed in women scheduled for ICSI. The study arm was injected with intrauterine LMWH during mock embryo transfer immediately following the ovum pickup procedure, while the control arm was given an intrauterine injection with a similar volume of tissue culture media. Side effects, the clinical pregnancy rate, and the implantation rate were recorded. Results: The pregnancy rate was acceptable (33.9%) in the LMWH arm with no significant reported side effects, confirming the safety of the intervention. No statistically significant differences were found in the clinical pregnancy and implantation rates between both groups (p= 0.182 and p= 0.096, respectively). The odds ratio of being pregnant after intrauterine injection with LMWH compared to the control group was 0.572 (95% confidence interval [CI], 0.27-1.22), while the risk ratio was 0.717 (95% CI, 0.46-1.13; p= 0.146). No statistical significance was found between the two groups in other factors affecting implantation, such as day of transfer (p= 0.726), number of embryos transferred (p= 0.362), or embryo quality. Conclusion: Intrauterine injection of LMWH is a safe intervention, but the dose used in this study failed to improve the outcome of ICSI. Based on its safety, further research involving modification of the dosage and/or the timing of administration could result in improved ICSI success rates.

Adaptive Fourier Series Neural Network PID Controller

Mohamed Benrabah,Kamel Kara,Oussama AitSahed,Mohamed Laid Hadjili 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.10

In this work, an Adaptive Neural Networks PID controller structure, called Adaptive Fourier Series Neural Networks PID controller (AFSNNPID), is developed. The main objective is to obtain a simple controller for nonlinear systems that can be tuned online to reject perturbations effect and compensate the system parameters variation. Due to its simple architecture and very attractive proprieties, the Fourier Series Neural Network (FSNN)is used to online adjust the parameters of the PID controller. Furthermore, using the delta-rule algorithm, the adaptation dynamics of the FSNN is globally stable.The design procedure of the proposed controller and the stability analysis of the closed loop system using the small gain theorem are given. To assess the effectiveness of the proposed control scheme, the control of a 3-DOF robot arm manipulator is considered and a comparative study, usingthe adaptive neural network PID controller and the particle swarm optimization based PID controller, is carried out. The obtained results, through the experimental study, indicate that the AFSNNPID controller presents better control performance than the other controllers

Biology of the mason bee Osmia latreillei (Hymenoptera: Megachilidae) under artificial nesting conditions in Egypt

Mohamed A. Shebl,Hanan A. Hassan,Soliman M. Kamel,Mohamed A.M. Osman,Michael S. Engel 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3

The mason bee Osmia (Helicosmia) latreillei Spinola is one of the dominant species of bees throughout Egypt, and can be found commonly in the Suez Canal Region. The species visits numerous plant species of the family Asteraceae and is considered the most important pollinator of certain crops such as sunflower. This species and some other solitary cavity nesting bees are threatened by fragmentation of their nesting habitats. Several attempts were made to establish and propagate O. latreillei, and success was achieved for re-nesting it in various artificial materials such as wood and polystyrene with rolled paper straws. These artificial nests were established at the conservation area of the Bee Research Centre, Suez Canal University, in order to study the nesting behavior and biology of O. latreillei under artificial conditions and to document their natural enemies. Biologically, there was no significance difference between the life cycle of O. latreillei under natural and artificial nesting conditions. This solitary bee was univoltine, individuals active only during Spring, and attacked by diverse enemies, with Stelis (Stelis) murina Pérez and chrysidid wasps considered the two most major pests of this wild bee species.

Original Article: Food Science/Microbiology : Optimization of Medium Composition and Cultivation Parameters for Fructosyltransferase Production by Penicillium aurantiogriseum AUMC 5605

( Mohamed Abdel Fattah Mohamed Farid ),( Zinat Kamel ),( Elsayed Ahmed Elsayed ),( Azza Mohamed Noor El Deen ) 한국응용생명화학회 2015 Journal of Applied Biological Chemistry (J. Appl. Vol.58 No.3

Fructooligosaccharides have been mainly produced by microbial fructosyltransferases (FTase) enzymes. The present work focuses on the optimization of medium composition and cultivation parameters affecting FTase produced by Penicillium aurantiogriseum AUMC 5605 in shake flask cultivation. FTase production was optimized in two steps using DeMeo’s fractional factorial design. A 1.46-fold increase in FTase production (105.4 U/mL) was achieved using the optimized culture medium consisting of (g/L): sucrose, 600; yeast extract, 10; K2HPO4, 5; MgSO4·7H2O, 0.5; (NH4) 2SO4, 1.0 and KCl, 0.5. The obtained results showed that the maximum FTase enzyme activity was produced at initial cultivation pH values ranging from 6.0-6.5, at agitation speed of 200 rpm and using vegetative fungal cells as inoculum. Moreover, results showed that optimization of medium composition and some cultivation parameters resulted in an increase of about 93.7% in the enzyme activity than the nonoptimized cultivation conditions after 96 h of cultivation. Additionally, maximum production and specific production rates recorded 2340 U/L/h and 102 U/L/h/g cells, respectively.

Therapeutic Potential of Artichoke in the Treatment of Fatty Liver: A Systematic Review and Meta-Analysis

Ahmed Mohamed Kamel,Mohamed Ali Farag 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.10

Nonalcoholic fatty liver disease (NAFLD) is a major chronic liver disease that can lead to liver cirrhosis, liver cancer, and death. Artichoke leaf extract (ALE) is well known in folk medicine for its hepatoprotective effect. Till recent times, no sufficient data from randomized clinical trials (RCTs) exist to support such use. This meta-analysis summarizes evidence from recent RCTs that evaluated ALE in NAFLD patients. Electronic databases were searched for RCTs that used ALE in NAFLD patients. The random-effects model was used to pool effect sizes (standardized change score). Data synthesis from five RCTs (333 patients) showed that ALE resulted in a significant reduction in alanine aminotransferase (standardized mean difference [SMD]: 1.1; 95% confidence interval [CI], 0.79–1.73; P < .001) and aspartate aminotransferase levels (SMD: 1.01; 95% CI, 0.52–1.51; P < .001) compared with the control group. ALE also resulted in a significant reduction in total cholesterol (SMD: 0.98; 95% CI, 0.53–1.43; P = .004), low-density lipoprotein (SMD: 0.96; 95% CI, 0.3–1.62; P < .001) and triglycerides (SMD: 0.95; 95% CI, 0.58–1.32; P < .001). The current review provides evidence from RCTs to support the use of ALE as a hepatoprotective agent in NAFLD patients. The study was registered on the PROSPERO database with the Registration No. CRD42020182502 (https://www.crd.york.ac.uk/prospero).

Efficacy of Extracorporeal Shockwave Therapy on Cervical Myofascial Pain Following Neck Dissection Surgery: A Randomized Controlled Trial

FatmaAlzahraa Hassan Kamel,Maged Basha,Ashwag Alsharidah,Islam Mohamed Hewidy,Mohamed Ezzat,Nancy Hassan Aboelnour 대한재활의학회 2020 Annals of Rehabilitation Medicine Vol.44 No.5

Objective To investigate the efficacy of extracorporeal shockwave therapy (ESWT) on cervical myofascial pain following neck dissection in reducing pain and improving cervical range of motion (ROM). Methods Forty-six patients with cervical myofascial pain following neck dissection surgery were recruited and subdivided at random into two equal groups. The ESWT group received ESWT once a week for 4 weeks (0.25 mL/mm2, 1,000 shocks) and a topical non-steroidal anti-inflammatory drug (3 times/day for 4 weeks). The control group received only topical NSAID. The pain assessment was done by using the visual analog scale (VAS) and pressure algometry. A cervical ROM device was used for the assessment of the lateral flexion and rotation of the neck ROM on both sides. All measurements were collected at baseline, 2 weeks, and 4 weeks. Results The ESWT group revealed a significant improvement in all parameters at post I and post II than did the control group (p>0.001), that revealed a statistical decrease only in the VAS score at post I without any statistical difference in the pain threshold and neck ROM. However, there were statistical differences in all parameters at post II compared to those at pre-treatment and post I (p<0.001). Conclusion As a confirmation of the efficacy of ESWT in cervical myofascial pain control following neck dissection, we observed better results with no side effects in the ESWT group (Clinical Trial Registry No. PACTR202002648274347).

Biodegradation and decolorization of azo dyes by adherent Staphylococcus lentus strain

Kamel Chaieb,Mohamed Hagar,Nagi R. E. Radwan 한국응용생명화학회 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.3

A Staphylococcus lentus strain, isolated from Red sea water, was tested for decolorization capacity of Congo red, Evans blue, and Eriochrome Black T azo dyes. Biodegradation (100 mgl−1) of these dyes was studied within 24 h in Mineral Salt Medium solution containing 0.10 % (w/v) yeast extract and 7 mM of glucose at a pH of 7.2 and a temperature of 37 °C. Using a 2.2 × 106 CFU/mL inoculum size, S. lentus was able to decolorize these azo dyes with different degree. The staphylococcal biomass achieves approximately 100 % decolorization of Congo red and Eriochrome Black T by the end of treatment. FTIR and UV–Vis analyses confirm biodegradation potential of the tested strain. Moreover, the phytotoxicity of the dye solutions resulting from this treatment shows lower toxic nature compared to untreated solution of the respective dyes.

Ordinal Pattern Analysis Method Applied in a P300-based Brain Computer Interface

Mohammed J. Alhaddad,Mahmoud I Kamel,Dalal M. Bakheet 보안공학연구지원센터 2014 International Journal of Multimedia and Ubiquitous Vol.9 No.6

Characterization of Induced Polarization Parameters from Electromagnetic Data Using Evolutionary Approach

Mohamed Elkattan,Aladin H. Kamel 한국전자파학회 2023 Journal of Electromagnetic Engineering and Science Vol.23 No.4

Electromagnetic methods are one of the most important tools for exploring the physical properties of scatterers. When the scattering object is polarizable, more information can be gained from electromagnetic measurements to determine the scatterer’s electrical properties. In this paper, an inversion scheme is introduced to extract induced polarization information from electromagnetic measurements. The inverse scattering problem is reformulated as an optimization problem that utilizes an efficient forward model solver to compute the scattered field. Furthermore, a simulated annealing approach is considered in the presented scheme, wherein different cooling schedules are evaluated to solve the inverse problem. The results of applying the proposed scheme to various case studies are reported.