Pseudomonas syringae pv. tomato DC3000 Improves Escherichia coli O157:H7 Survival in Tomato Plants

( Min Namgung ),( Yeon-jeong Lim ),( Min Kyu Kang ),( Chang-sik Oh ),( Duck Hwan Park ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.12

Recently, outbreaks of food-borne diseases linked to fresh produce have been an emerging public health concern worldwide. Previous research has shown that when human pathogens co-exist with plant pathogens, they have improved growth and survival rates. In this study, we have assessed whether Escherichia coli O157:H7 benefits from the existence of a phytopathogenic bacterium and the underlying mechanisms were further investigated. When Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and E. coli O157:H7 were co-inoculated by either dipping or infiltration methods, the populations of E. coli O157:H7 increased; however, no effect was observed when type three secretion system (T3SS) mutants were used instead, suggesting that E. coli O157:H7 benefits from the presence of Pst DC3000. In addition, this study confirmed that the E. coli O157:H7 populations increased when they occupied the tomato leaf intercellular space; this colonization of the interior of the leaves was possible due to the suppression of the PAMP-triggered immunity (PTI) by Pst DC3000, in particular with the AvrPto effector. In conclusion, our data support a plausible model that E. coli O157:H7 benefits from the presence of Pst DC3000 via AvrPto suppression of the PTI resistance.

Evaluation of bacterial stalk rot resistance lines screening of Dickeya zeae in maize

Min Namgung,Moonjong Kim,Sihwan Ryu,Jaekeun Choi,Jongyeol Park,Seungchul Choi,Woosik Yong,Gyeongnam Nam,Inseok Seo,Junkeun Choi 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10

국내 유기상추의 생산환경 조사분석

남궁민(Min Namgung),김범석(Beom Seok Kim),허성진(Seong Jin Heo),최용범(Yong Beom Choi),허장현(Jang Hyun Hur),박덕환(Duck Hwan Park) 한국농약과학회 2014 농약과학회지 Vol.18 No.2

Validation and Application of a Real-time PCR Protocol for the Specific Detection and Quantification of Clavibacter michiganensis subsp. sepedonicus in Potato

Cho, Min Seok,Park, Duck Hwan,Namgung, Min,Ahn, Tae-Young,Park, Dong Suk The Korean Society of Plant Pathology 2015 Plant Pathology Journal Vol.31 No.2

Clavibacter michiganensis subsp. sepedonicus (Cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. Therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. Although several PCR assays have been developed for detection, they cannot afford specific detection of Cms. Therefore, in this study, a computational genome analysis was performed to compare the sequenced genomes of the C. michiganensis subspecies and to identify an appropriate gene for the development of a subspecies-specific PCR primer set (Cms89F/R). The specificity of the primer set based on the putative phage-related protein was evaluated using genomic DNA from seven isolates of Cms and 27 other reference strains. The Cms89F/R primer set was more specific and sensitive than the existing assays in detecting Cms in in vitro using Cms cells and its genomic DNA. This assay was also able to detect at least $1.47{\times}10^2copies/{\mu}l$ of cloned-amplified target DNA, 5 fg of DNA using genomic DNA or $10^{-6}$ dilution point of 0.12 at $OD_{600}$ units of cells per reaction using a calibrated cell suspension.

Cardiac Myxoma Detachment Presenting with Abdominal Aortic Total Occlusion

Choe, Hyun-Min;Park, Min-Yong;Kim, Na-Kyung;Lee, Ki-Jun;Jang, Woo-Ik;Kim, Chang-Young;Park, Kyung-Taek;Doh, Joon-Hyung;Namgung, June;Lee, Sung-Yun 인제대학교 2011 仁濟醫學 Vol.32 No.-

심장 점액종 환자에 있어서 점액중의 색전에 의해 복부 대동맥이 폐쇄되는 경우는 아주 드문 합병증으로 지금까지 보고된 바가 드물다. 현재까지 심장 점액중의 병태 생리 및 자연경과에 대해서는 잘 알려져 있옴에도 불구하고 점액중의 분리 에 의한 혈관 색전증은 예상치 못한 심각한 합병증을 초래 할 수 있다. 본 증례는 심장 점액종 색전에 의해 복부 대동맥폐쇄가 발생한 환자에서 수술적 색전 제거술 및 점액중 절제술로 치료한 경험을 보고 하고자 한다.

High Incidence of Rickets in Extremely Low Birth Weight Infants with Severe Parenteral Nutrition-Associated Cholestasis and Bronchopulmonary Dysplasia

Lee, Soon Min,Namgung, Ran,Park, Min Soo,Eun, Ho Sun,Park, Kook In,Lee, Chul The Korean Academy of Medical Sciences 2012 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.27 No.12

<P>Risk factors for rickets of prematurity have not been re-examined since introduction of high mineral formula, particularly in ELBW infants. We analyzed the incidence and the risk factors of rickets in extremely low birth weight (ELBW) infants. As a retrospective case-control study from 2004 to 2008, risk factors were analyzed in 24 patients with rickets versus 31 patients without. The frequency of rickets in ELBW infants was 24/55 (44%). Infants with rickets were diagnosed at 48.2 ± 16.1 days of age, and improved by 85.3 ± 25.3 days. By radiologic evaluation, 29% were grade 1 rickets, 58% grade 2 and 13% grade 3. In univariate analysis, infants with rickets had significantly higher incidence of patent ductus arteriosus, parenteral nutrition associated cholestasis (PNAC), severe PNAC and moderate/severe bronchopulmonary dysplasia (BPD). In multiple regression analysis, after adjustment for gestation and birth weight, rickets significantly correlated with severe PNAC and with moderate/severe BPD. Serum peak alkaline phosphatase levels were significantly elevated in rickets (<I>P</I> < 0.001). In ELBW infants, the incidence of rickets of prematurity remains high and the incidence of severe PNAC and moderate/severe BPD was significantly increased 18 and 3 times, respectively.</P>

신생아기에 진단된 미토콘드리아 호흡 사슬 결함 1례

최경민 ( Kyoung Min Choi ),권해식 ( Hae Sik Kweon ),이동우 ( Dong Woo Lee ),남궁란 ( Ran Namgung ),박민수 ( Min Soo Park ),이철 ( Chul Lee ),이영목 ( Young Mok Lee ),이진성 ( Jin Sung Lee ) 대한주산의학회 2003 Perinatology Vol.14 No.1

미토콘드리아 대사질환은 지방산 산화장애, 피루브산염 대사장애 및 호흡사슬 결함으로 분류되는데, 호흡 사슬 결함의 신생아기 임상증상은 반복적인 무호흡, 경련이 동반된 혼수, 선천성 젖산혈증, 긴장저하, 간기능부전, 비후형 심근증 등이며, 진단은 세포질과 미토콘드리아의 산화환원 상태를 반영하는 지표인 혈장의 젖산, 피루브산염, 케톤체의 측정과 그 molar ratios로 한다. 본 저자등은 신생아에서 심한 대사성 산증과 동반되어 혼수, 호흡부전, 간기능장애, 신부전증 등 다발성 장기 기능 부전소견을 보이며, 젖산 산혈증(>2.5mM), 증가된 젖산/피루브산염 비(>20), 케톤뇨 등이 확인되어 선천성 대사이상 중 미토콘드리아 호흡사슬 결함으로 진단된 1례를 보고한다. Mitochondrial diseases are classified into the three major categories, defects of fatly acid oxidation, defects of pyruvate metabolism, and defects of the respiratory chain, and all of these cause severe neuron-logic dysfunction in the newborn period. Defects of the mitochondrial respiratory chain present as recurrent apnea, seizures, congenital lactic acidosis, hypotonia, hepatic dysfunction and hypertrophic cardiomyopathy in the neonatal period. Laboratory findings of hyperlactataemia (>2.5mM), elevated lactate/ pyruvate (L/P) ratio (>20) and ketone body ratio (>2) suggest the diagnosis of mitochondrial respiratory chain defects. We report a case of mitochondrial respiratory chain defect diagnosed in the neonatal period presenting with multiorgan failure consisting of severe metabolic acidosis, comatous mental state, respiratory distress, hepatic dysfunction, renal failure with lactic acidosis (24mM), increased L/P ratios (55.6) and ketonuria (increased ratio of 3-hydroxybutyrate/acetoacetate).

임상 ; 한국인 모유의 영양소와 영아의 성장과의 관계 분석 연구

이철 ( Chul Lee ),박민수 ( Min Soo Park ),박국인 ( Kook In Park ),남궁란 ( Ran Namgung ),이순민 ( Soon Min Lee ),민경복 ( Kyong Bok Min ),은호선 ( Ho Sun Eun ) 대한주산의학회 2012 Perinatology Vol.23 No.4

목적 : 본 연구는 건강한 수유부와 정상 만삭아를 대상으로 모유의 조성이 영아 성장에 미치는 영향을 확인하고자 하 였다. 방법 : 2011년 10월부터 2012년 3월까지 강남세브란스병원, 서울의 수유 센터 두 곳을 방문한 건강한 수유부와 영 아 173명을 대상으로 하였다. 수집된 모유는 Semisolid-state mid-infrared milk analyzer (MIRIS(R) Human Milk Analyzer, HMA, Miris AB, Uppsala, Sweden)를 이용하여 모유 1 mL를 1분간 분석기에 넣어 측정하였다. 결과 : 모유 성분의 분석을 통하여 섭취량/권장량(%)을 표준 체중 5 percentile 이상인 군과 5 percentile 미만인 군으로 나누어 비교한 결과, 열량은 130.2±34.1%, 68.2±31.7%, 단백질은 133.4±33.9%, 75.0±28.3%, 지방질은 189.6±94.7 %, 91.9±63.2%, 탄수화물은 120.7±17.6%, 72.3±26.5%으로 두 군간에 유의한 차이를 보였다(P<0.05). 표준 체중 25 percentile 이상인 군과 25 percentile 미만인 군으로 나누었을 때도 열량은 135.3±34.3%, 99.0±33.9% 이었으며, 단백 질은 136.7±35.0%, 108.0±33.9%, 지방질은 197.1±97.6, 141.6±79.8%, 탄수화물은 125.1±16.5%, 95.0±22.0%으로 두 군간에 유의한 차이를 보였다(P<0.05). Multiple logistic regression을 이용하여 분석한 결과 표준 체중이 5% 미만인 군에 영향을 미치는 인자는 탄수화물이 가장 유의한 인자이었고(P<0.05), 25% 미만인 군에 영향을 미치는 인자는 단백 질과 탄수화물이었다(P<0.05). 결론 : 본 연구에서는 영아의 체중이 작은 경우 모유 성분의 영양소가 유의하게 낮음을 확인하였다. 이를 통해 출생 후 체중 증가가 유의하게 적고, 성장 곡선상 낮은 체중 분포를 보일 경우 적극적인 모유수유와 더불어 수유부의 식습관 개 선을 통한 적정한 영양공급 및 나아가 영양 강화를 이루어 신생아의 성장 증가를 도모해야 하겠다. Purpose : The aim of this study was to determine the effect of the macronutrient composition of breast milk from Korean women on the growth of infants. Methods : 173 healthy lactating women and breast-fed infants who visited Gangnam Severance Hospital and two breast-feeding centers in Seoul from October 2011 to March 2012 were recruited. We checked the birth weight and body weight of infants while collecting breast milk from the mothers, and analyzed the macronutrient component of breast milk with a mid-infrared milk analyzer (MIRIS(R) Human Milk Analyzer, HMA, Miris AB, Uppsala, Sweden). Group analysis was performed depending on more or less than 5 percentile and 25 percentile of body weight. Results : The amount of daily intake/RDA for calories, proteins, lipids and carbohydrates of breast milk were significantly lower in the less than 5 percentile and 25 percentile group (P <0.05). Using multiple logistic regression analysis, the significant nutrient component that was insufficient in the less than 5 percentile and less than 25 percentile of body weight group respectively was carbohydrates (P <0.05). Conclusion : We conclude that each macronutrient level of breast milk is statistically low in infants with less body weight. These results suggest that nutritional consideration of breast milk is necessary for the growth of breast-fed infants.

Usefulness of serum cystatin C to determine the dose of vancomycin in neonate

Shin, Jeong Eun,Lee, Soon Min,Eun, Ho Seon,Park, Min Soo,Park, Kook In,Namgung, Ran The Korean Pediatric Society 2015 Clinical and Experimental Pediatrics (CEP) Vol.58 No.11

Purpose: The vancomycin dosage regimen is regularly modified according to the patient's glomerular filtration rate (GFR). In the present study, we aimed to assess the usefulness of serum cystatin C (Cys-C) concentration, compared with serum creatinine (SCr) concentration, for predicting vancomycin clearance (CLvcm) in neonates. Methods: We retrospectively analyzed the laboratory data of 50 term neonates who were admitted to the neonatal intensive care unit and received intravenous vancomycin, and assessed the pharmacokinetic profiles. Creatinine clearance (CLcr) and GFR based on Cys-C (GFRcys-c) were estimated using the Schwartz and Larsson formulas, respectively. Results: The mean CLvcm (${\pm}$standard deviation) was $74.52{\pm}31.17L/hr$, the volume of distribution of vancomycin was $0.67{\pm}0.14L$, and vancomycin half-life was $9.16{\pm}17.42hours$. The SCr was $0.46{\pm}0.25mg/dL$ and serum Cys-C was $1.43{\pm}0.34mg/L$. The peak and trough concentrations of vancomycin were $24.65{\pm}14.84$ and $8.10{\pm}5.35mcg/mL$, respectively. The calculated GFR based on serum creatinine concentration (GFR-Cr) and GFRcys-c were $70.2{\pm}9.45$ and $63.6{\pm}30.18mL/min$, respectively. The correlation constant for CLvcm and the reciprocal of Cys-C (0.479, P=0.001) was significantly higher than that for CLvcm and the reciprocal of SCr (0.286, P=0.044). GFRcys-c was strongly correlated with CLvcm (P=0.001), and the correlation constant was significantly higher than that for CLvcm and CLcr (0.496, P=0.001). Linear regression analysis showed that only GFRcys-c was independently and positively correlated with CLvcm (F=41.9, P<0.001). Conclusion: The use of serum Cys-C as a marker of CLvcm could be beneficial for more reliable predictions of serum vancomycin concentrations, particularly in neonates.

The efficacy and safety of Montelukast sodium in the prevention of bronchopulmonary dysplasia

Kim, Sang Bum,Lee, Jang Hoon,Lee, Juyoung,Shin, Seung Han,Eun, Ho Sun,Lee, Soon Min,Sohn, Jin A,Kim, Han Suk,Choi, Byung Min,Park, Min Soo,Park, Kook In,Namgung, Ran,Park, Moon Sung The Korean Pediatric Society 2015 Clinical and Experimental Pediatrics (CEP) Vol.58 No.9

Purpose: The purpose of this study was to evaluate the efficacy and safety of Montelukast sodium in the prevention of bronchopulmonarydysplasia (BPD). Methods: The Interventional study was designed as a multicenter, prospective, and randomized trial, with open labeled and parallel-experimental groups, 66 infants were enrolled and allocated to either the case group (n=30) or the control group (n=36) based on gestational age (GA). Infants in the case group were given Montelukast sodium (Singulair) based on their body weight (BW). Zero week was defined as the start time of the study. Results: The incidence of moderate to severe BPD was not different between the groups (case group: 13 of 30 [43.3%] vs. control group: 19 of 36 [52.8%], P=0.912). Additionally, secondary outcomes such as ventilation index, mean airway pressure and resort to systemic steroids were not significantly different. There were no serious adverse drug reactions in either group, and furthermore the rate of occurrence of mild drug related-events were not significantly different (case group: 10 of 42 [23.8%] vs. control group: 6 of 48 (15.8%), P=0.414). Conclusion: Montelukast was not effective in reducing moderate or severe BPD. There were no significant adverse drug events associated with Montelukast treatment.