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Mikes, Jaromir,Koval, Jan,Jendzelovsky, Rastislav,Sackova, Veronika,Uhrinova, Ivana,Kello, Martin,Kulikova, Lucia,Fedorocko, Peter Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.11
Photodynamic therapy with hypericin (HY-PDT) is known as an efficient modality for treatment of various cancerous and non-cancerous diseases. Although the role of p53 protein in cell death signaling is well established, relatively little is known of its impact on the efficiency of HY-PDT. Comparison of sensitivity and long-term survival of p53-null versus wt-p53-expressing HCT-116 cells is reported here. The lack of p53 function did not affect cell proliferation or attenuate the initial phases of programmed cell death. However, analyses of apoptosis in the final stages revealed suppression of its incidence and delayed activation of caspase-3 in p53-null cells. Significantly higher clonogenic ability, especially in hypoxia, was identified in the case of p53-null cells. Induction of Mcl-1 and Bax levels were more prominent in wt-pt53 cells. Interestingly, the level of Bcl-2 did not react to HY-PDT at all, in both cell lines. Bringing the evidence together, we prove that despite insignificant impact on overall toxicity, expression of p53 affects the clonogenic efficiency of HCT-116 cells. Since destruction of tumor tissue and its vascular system by PDT tends to lead to hypoxia, superior survival of p53-deficient tumor cells under given conditions might result in recurrence of cancer diseases.
Jendzelovsky, Rastislav,Mikes, Jaromir,Koval, Jan,Soucek, Karel,Prochazkova, Jirina,Kello, Martin,Sackova, Veronika,Hofmanova, Jirina,Kozubik, Alois,Fedorocko, Peter Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.12
Photodynamic therapy (PDT) is a flexible multi-target therapeutic approach. One of the main requirements of successful PDT is sufficient intracellular concentration of an applicable photosensitizer. Mechanisms of anticancer drug elimination by tumour cells are mostly linked to the elevated expression and activity of P-glycoprotein (P-gp), multidrug resistance-associated protein 1 (MRP1), breast cancer resistance protein (BCRP) and P450 monooxygenases. The interaction of hypericin with this cell drug-defence system is still unclear. We report here for the first time increased activity of MRP1 and BCRP in HT-29 colon cancer cells treated with hypericin per se. On the contrary, pre-treatment with proadifen (SKF525A) affected the function of MRP1 and BCRP leading to increased hypericin content, which might indicate a possible link between proadifen and these ABC transporter proteins. Subsequent enhanced intracellular oxidative stress was accompanied by loss of mitochondrial membrane potential, activation of caspase-9 and -3, PARP cleavage and onset of apoptosis. In conclusion, our study suggests that drug efflux transporters MRP1 and BCRP affect the pharmacokinetics of hypericin in HT-29 colon adenocarcinoma cells, and the action of hypericin-mediated PDT (HY-PDT) should be modulated by pre-treatment with their specific inhibitors.
Efremova, Olga A.,Brylev, Konstantin A.,Vorotnikov, Yuri A.,Vejsadová,, Lucie,Shestopalov, Michael A.,Chimonides, Gwen F.,Mikes, Petr,Topham, Paul D.,Kim, Sung-Jin,Kitamura, Noboru,Sutherland, A The Royal Society of Chemistry 2016 Journal of Materials Chemistry C Vol.4 No.3
<▼1><P>Transparent yet photoluminescent, materials with facile processability are key in many applications.</P></▼1><▼2><P>Materials that combine photoluminescence, optical transparency and facile processability are of high importance in many applications. This article reports on the development of photoluminescent poly(methyl methacrylate) materials based on novel highly emissive anionic molybdenum cluster complex [{Mo6I8}(OTs)6]<SUP>2−</SUP> (where OTs<SUP>−</SUP> is the <I>p</I>-toluenesulfonate ion). The materials were obtained by both solution and bulk copolymerisation of methyl methacrylate and (dMDAEMA)2[{Mo6I8}(OTs)6], where dMDAEMA<SUP>+</SUP> is the polymerisable cation [2-(methacryloyloxy)ethyl]dimethyl-dodecylammonium. Evaluation of the resultant hybrid materials showed that one could combine the excellent photoluminescent properties of the cluster complex with the transparency and processability of PMMA.</P></▼2>
Mike Day(마이크 데이),Jeong-Hee Hong(홍정희) 유공압건설기계학회 2012 드라이브·컨트롤 Vol.9 No.2
This paper explains how eliminating contamination from the manufacturing processes will lead to better product quality and hence the need for reworking, a trouble free commissioning period and greatly improved production efficiency. All of these will reduce costs and energy usage. It will also ensure that the product is delivered to the customer in a condition that will ensure improved reliability and longer life, again reducing both energy and other operating costs. Correctly designing the contamination control measures will achieve and maintain the level of fluid cleanliness that is required by the end user. The filter is critical to cleanliness management and should be selected with the same degree of thought and consideration as for other major components. This paper explains the role that Cleanliness Management plays in the reducing the carbon footprint of systems and processes by making them perform more efficiently for longer periods. It also examines two differing ways of selecting filters to incorporate the features of newer designs, and shows how significant savings in the costs of ownership can be achieved using these approaches.