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- 저자 : Meichen Guo (검색결과 2 건)
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Meichen Liu,Yu Zhao,Baojin Yao,Hui Zhang,Huanyu Guo,Dongyang Hu,Qun Wang 한국유전학회 2014 Genes & Genomics Vol.36 No.5
The most commonly used normalization strategyfor quantitative real-time reverse transcription-polymerasechain reaction (RT-qPCR) is to select a stablereference gene. However, to date, no suitable referencegenes have been identified in sika deer antler tissues. Thus,the aim of this study was to identify the most stable gene ora set of genes to be used as reference genes for RT-qPCRanalysis in sika deer antler tissues. We first selected candidatereference genes using sika deer antler gene expressiondata from an Illumina sequencing platform (Hiseq2000); twenty-one reference genes from the antler tips ofChinese sika deer were selected to test for the normalizationof expression levels during different growth stages. These genes were tested by RT-qPCR and ranked accordingto the stability of their expression using two differentmethods (implemented in geNorm and NormFinder). Based on different algorithms and analytical procedures,our results clearly indicate RPL40 and Gpx as the moststable reference genes of our pool.
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Baoping Zhang,Yun Liu,Zhongchen Ma,Meichen Guo,Bowen Shen 한국고분자학회 2018 Macromolecular Research Vol.26 No.2
The chemically modified lignin was synthesized based on rice straw and the adsorption properties of AuCl4 - were investigated in this paper. The adsorbent was characterized by Fourier transform infrared spectrometer, scanning electronic microscope and thermogravimetric analysis and differential scanning calorimetry. The chemically modified rice straw lignin was of excellent thermal stability and had plenty of primary amines and hydroxyls. Many influence factors such as initial concentration of AuCl4 -, adsorption time and concentration of hydrochloric acid on AuCl4 - were optimized. The maximum adsorption capacity was 783.94 mg·g-1. The equilibrium adsorption time was 600 min. The equilibrium isotherm fitted well with the Langmuir model and the pseudo-second-order kinetic model was suitable for describing the adsorption of AuCl4 -. Low hydrochloric acid concentration could promote the selectivity of adsorption on AuCl4 -. Combination of ion exchange and reduction was the mechanism. AuCl4 - was adsorbed by ion exchange first and then reduced from hydrochloric acid medium in the form of Au(0) by phenolic hydroxyl.
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