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RISS 인기검색어
- 검색키워드
- 저자 : Md. Ramjan Ali (검색결과 5 건)
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Md. Ramjan Ali,문정용,Rajendra Gyawali,Ashik Mosaddik,Youn-Chul Ryu,김소미 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.3
Flesh and peel extracts of five mango varieties were compared for their antioxidant and antiproliferative activities. Fozli peel (FP) extract was found to have the highest antioxidant activity and to significantly inhibit the proliferation of HeLa human cervical carcinoma cells in a dose-dependent manner. Hoechst 33342 staining revealed apoptotic nuclei with condensed chromatin, and cell growth arrest was confirmed by flow cytometry. Apoptotic signaling induced by FP extract was characterized by an increased Bax/Bcl-2 ratio and down-regulation of Bcl-2. Moreover, FP extract treatment triggered the proteolytic activation of caspases-3, -8, and -9 and the degradation of poly ADP-ribose polymerase in HeLa cells. These results indicate that FP is an excellent source of phenolic and/or flavonoid compounds, and may have applications in the treatment of human cervical carcinoma and as an antioxidant in the food and pharmaceutical industries. Gas chromatography-mass spectrometry analysis of FP extract revealed that 2,5-dihydroxyphenol was detected with highest amount and other compounds such as 5-hydroxymethyl-2-furancarboxaldehyde, pentadecanoic acid, (Z,Z)-9,12-octadecadienoic acid, and 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one were also found in high amounts.
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Md. Ramjan Ali,Yonggyun Kim 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04
Teratocytes (TCs) are the cells derived from the embryonic serosal membrane of some parasitic hymenopteran insects. As a parasitic factor, TCs are multifunctional in host regulation by inducing nutritional, immune, and developmental alterations. However, little is understood about their genetic constituents. This study reveals a comprehensive view of the genes expressed by TCs through a transcriptome analysis based on RNAseq technology. More than 6.29 Gb sequences were used to assemble 34,686 contigs (>200 bp) and annotated into different functional categories. The TC transcriptome profile was clearly distinct from those of hemocytes and the fat body. The TC transcriptome contained components of insulin signaling and biosynthesis of juvenile hormone and 20-hydroxyecdysone. TCs also expressed various groups of digestive enzymes, supporting its nutritional role for the growing parasitoid larvae in parasitism. Furthermore, this transcriptome analysis annotated two kinds of immunosuppressive serine protease inhibitors (serpins) and Rho GTPase-activating proteins (RhoGAPs). To determine the biological functions of these factors, we devised ex vivo RNA interference (RNAi) by conducting knockdown of gene expression in in vitro cultured TCs followed by injection of the treated TCs to test insects. Ex vivo RNAi revealed that some serpins and RhoGAPs expressed in TCs inhibited host cellular immunity. This study reports a transcriptome of the unique TC animal cell, and its immunosuppressive genetic factors using ex vivo RNAi technology.
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Md. Ramjan Ali,Yonggyun Kim 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05
Teratocytes are originated from embryonic serosal membrane of some endoparasitoid wasps. Cotesia plutellae eggs release teratocytes in parasitoid host hemocoel at hatch in about 150 cells per egg. Teratocytes of C. plutellae were cultured in an insect culture medium for at least 14 days. Teratocytes cultured in vitro showed no increase in cell numbers but increased in cell size. Similarly,teratocytes in parasitized larvae did not increase cell numbers, but increased their cell size. Microinjection of invitro cultured teratocytes in to third instar larvae of nonparasitized Plutella xylostella showed a dose-dependently inhibitory effect on development and larval-pupal metamorphosis. In addition, teratocytes prolonged the immature developmental period and reduced the pupation rate, in which young aged host larvae were more sensitive to teratocytes treatment than old larvae. These results suggest that teratocytes play a crucial role in successful parasitization of C.plutellae by altering host developmental program.
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Md. Ramjan Ali,Yonggyun Kim 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10
An endoparasitoid wasp, Cotesia plutellae, parasitizes young larvae of the diamondback moth, Plutella xylostella, with its parasitic factors of polydnavirus, venom, ovarian proteins, and teratocytes (TC). TCs are originated from embryonic serosal membrane at hatch of C. plutellae eggs. TCs, after released in hemocoel of parasitized larvae, increased their average cell size from 20.6 μm to 77 μm during whole developmental period of the parasitoid larvae, but did not increase their cell number by maintaining about 150 cells per larvae. TCs of C. plutellae, are considered to be involved to extend the host larval development period and to arrest larval-pupal metamorphosis, were cultured in an insect cell culture medium for 21 days. Like TCs in parasitized larvae, in vitro cultured TCs showed increase in cell size, but did not show increase of cell number. Microinjection of in vitro cultured TCs significantly inhibited larva-to-pupa metamorphosis of nonparasitized P. xylostella, in which pupated host also showed extended larval period. Larvae injected with TCs exhibited alteration in expression of ecdysone receptor (EcR) and insulin receptor (InR) as well as in parasitized larvae. Teratocyte-secretory factors in culture medium showed this antimetamorphic effect on P. xylostella, while heat treated TC culture medium lost the effect. However, a successful parasitization of C. plutellae required both TCs and polydnavirus to alter host physiology.
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Ali, Md. Ramjan,Yong, Moon-Jeong,Gyawali, Rajendra,Mosaddik, Ashik,Ryu, Youn-Chul,KimCho, So-Mi The Korean Society for Applied Biological Chemisty 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.58 No.3
Flesh and peel extracts of five mango varieties were compared for their antioxidant and antiproliferative activities. Fozli peel (FP) extract was found to have the highest antioxidant activity and to significantly inhibit the proliferation of HeLa human cervical carcinoma cells in a dose-dependent manner. Hoechst 33342 staining revealed apoptotic nuclei with condensed chromatin, and cell growth arrest was confirmed by flow cytometry. Apoptotic signaling induced by FP extract was characterized by an increased Bax/Bcl-2 ratio and down-regulation of Bcl-2. Moreover, FP extract treatment triggered the proteolytic activation of caspases-3, -8, and -9 and the degradation of poly ADP-ribose polymerase in HeLa cells. These results indicate that FP is an excellent source of phenolic and/or flavonoid compounds, and may have applications in the treatment of human cervical carcinoma and as an antioxidant in the food and pharmaceutical industries. Gas chromatography-mass spectrometry analysis of FP extract revealed that 2,5-dihydroxyphenol was detected with highest amount and other compounds such as 5-hydroxymethyl-2-furancarboxaldehyde, pentadecanoic acid, (Z,Z)-9,12-octadecadienoic acid, and 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one were also found in high amounts.
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