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여러 종류의 동해방지제를 이용한 소 체외수정란의 동결 및 일단계 융해후의 체외배양 생존율 및 수태율
T. Suzuki,M. Takagi,M. Yamamoto,A. Boediono,S. Saha,H. Sakakibara,M. Oe 한국수정란이식학회 1997 한국수정란이식학회 학술대회 Vol.1997 No.2
치밀 난구세포로 둘러싸인 소 난자를 . 5% 배양기에시 5% superovulated cow serum(SCS)이 첨가된 m-TCM 199 medium 으로 시간 배양하였으며, 수정능이 획득된 정자와 체외수정하였다. 7일8일경의 수정란을 1.3M methyl cellosolve(MC), 1.1M diethylene glycol(DEG), 1.8M ethylene glycol(EG), 1.6M propylene glycol(PG) 및 1.1M 1,3-butylene glycol(BG) 용액에서 10분간 평형시킨 후 0.25 ml 스트로내에 장전하였다. 스트로를 의 alcohol bath freezer에 넣고 까지 /분 속도로 냉각, 식빙 후 10분간 정체시켰으며, /분 또는 /분으로 까지 냉각 후 스트로를 액체질소에 침지하여 보관하였다. 수정란이 들어있는 스트로를 온수에서 융해하였으며, 수정란을 TCM 199 medium 으로 옮긴 후 5% SCS가 첨가된 TCM 199 medium 에서 48시간 배양하였다. 수정란이 양호한 형태를 유지하며 나중의 발육단계로 진행된 것을 생존한 것으로 간주하였다. 각 종류의 동해방지제에서 동결된 수정란의 일부는 융해 후 동해방지제를 제거하지 않고 직접 비외과적으로 이식하였다. 동결-융해 후 동해방지제의 종류에 따른 탈출배반포 발달율은 EG 50.0%, MC 53.6%, DEG 56.9%, PG 58.0% 그리고 BG 11.5%였다. /분 또는 /분 으로 냉각한 수정란의 생존율은 두 그룹간에 유의적인 차이가 없었으나 (P<0.05), 탈출배반포 발달율은 -0.5분 /분(22.6%, 12/53)보다 /분(64.6%, 31/48) 냉각시에 유의적으로 높았다(P<0.01). 동해방지제의 종류에 따른 수정란의 수태율은 MC 48%(10/21). DEG 30%(3/10), EG 74%(20/27) 및 PG 40%(4/10) 였다. 이러한 결과로 보아 MC, DEG, EG 그리고 PG는 소의 체외수정란의 동결을 위한 동해방지제로서 이용될 수 있음을 보여주었다.
What Is the Ideal Core Number for Ultrasound-Guided Prostate Biopsy?
Renato Caretta Chambó,Fábio Hissachi Tsuji,Flávio de Oliveira Lima,Hamilto Akihissa Yamamoto,Carlos Márcio Nóbrega de Jesus 대한비뇨의학회 2014 Investigative and Clinical Urology Vol.55 No.11
Purpose: We evaluated the utility of 10-, 12-, and 16-core prostate biopsies for detectingprostate cancer (PCa) and correlated the results with prostate-specific antigen (PSA)levels, prostate volumes, Gleason scores, and detection rates of high-grade prostaticintraepithelial neoplasia (HGPIN) and atypical small acinar proliferation (ASAP). Materials and Methods: A prospective controlled study was conducted in 354 consecutivepatients with various indications for prostate biopsy. Sixteen-core biopsy specimenswere obtained from 351 patients. The first 10-core biopsy specimens were obtainedbilaterally from the base, middle third, apex, medial, and latero-lateral regions. Afterward, six additional punctures were performed bilaterally in the areas more lateralto the base, middle third, and apex regions, yielding a total of 16-core biopsyspecimens. The detection rate of carcinoma in the initial 10-core specimens was comparedwith that in the 12- and 16-core specimens. Results: No significant differences in the cancer detection rate were found between thethree biopsy protocols. PCa was found in 102 patients (29.06%) using the 10-core protocol,in 99 patients (28.21%) using the 12-core protocol, and in 107 patients (30.48%) usingthe 16-core protocol (p=0.798). The 10-, 12-, and 16-core protocols were comparedwith stratified PSA levels, stratified prostate volumes, Gleason scores, and detectionrates of HGPIN and ASAP; no significant differences were found. Conclusions: Cancer positivity with the 10-core protocol was not significantly differentfrom that with the 12- and 16-core protocols, which indicates that the 10-core protocolis acceptable for performing a first biopsy.
T. Suzuki,M. Takagi,M. Yamamoto,A. Boediono,S. Saha,H. Sakakibara,M. Oe 한국수정란이식학회 1997 한국수정란이식학회 학술대회 Vol.1997 No.2
Bovine oocytes surrounded with compact cumulus cells were cultured for 20 to 22 hours(, 5% ) in modified TCM-199 medium supplemented with 5% superovulated cow serum(SCS) and inseminated by in vitro capacitated spermatozoa. Day 7 to 8 embryos were equilibrated for 10 minutes in 1.3M methyl cellosolve(MC) <1.1M diethylene glycol(DEG), 1.8M ethylene glycol(EG), 1.6M propylene glycol(PG) and 1.1 M 1,3-butylene glycol(BG) solutions. They were then loaded into 0.25ml straws, placed into an alcohol bath freezer at , cooled from to at /minute, seeded, held for 10 minutes, and stored in liquid nitrogen. After thawing in water, the embryos wee rehydrated in TCM-199 medium and then cultured for 48 hours in TCM-199 plus 5% SCS. Embryos were considered viable if they progressed to later developmental stages with a good morphology. Some of the embryos frozen in each cryoprotectant were thawed and transferred non-surgically without removing the cryoprotectant. Hatched embryos survived freezing and one-step dilution as follows : EG(50.0%), MC(53.6%), DEG(56.9%), PG(58.0%) and BG(11.5%). The survival rate of embryos cooled at vs. /minute was not significantly different(P<0.05), however, blastocysts hatched most often (P<0.01) in vitro when cooled at a rate of /minute(64.6%), 31/48) than at /minute(22.6%, 12/53). Pregnancy rates resulting from embryos frozen in the different cryoprotectants were as follows : MC(48%, 10/21); DEG(30%, 3/10); EG(74%, 20/27); and PG(40%, 4/10). These results indicate that MC, DEG, EG and PG have utility as cryoprotectants for the freezing and thawing of IVF Bovine embryos.
Yamamoto, T .,Yamaguchi, M .,Hirota, M .,Inui, H . 대한금속재료학회(대한금속학회) 2000 METALS AND MATERIALS International Vol.6 No.6
Changes in microstructure and their effects on hydrogen sorption properties have been investigated as a function of the number of hydrogen sorption cycles in LaNi_5 and FeTi. For LaNi_5, while the desorption pressure does not significantly depend on the number of cycles, the absorption pressure drastically decreases between the first and second cycles. In addition to severe cracking and pulverization, numerous edge dislocations with Burgers vectors of the 1/3$lt;12 ̄10$gt;-type are introduced during the first absorption cycles. Both the introduction of numerous lattice defects and the formation of severe cracks seem to be completed during the first cycle, resulting in the reproducible P (pressure)-C (composition) characteristics for the subsequent cycles. In contrast, FeTi exhibits ever-changing P-C characteristics with cycle number. The introduction of dislocations with Burgers vectors of the $lt;100$gt;-type in the form of tangles and cracks occurs during each of absorption cycles with the extent to which this occurs being considerably smaller in each of absorption cycles of FeTi than in the first absorption cycle of LaNi_5. The difference in material response to hydrogenation seems to be closely related to the difference in ductility of these two intermetallics.