Ten-eleven translocation 1 mediating DNA demethylation regulates the proliferation of chicken primordial germ cells through the activation of Wnt4/β-catenin signaling pathway

Lu Yinglin,Li Ming,Cao Heng,Zhou Jing,Li Fan,Yu Debing,Yu Minli 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.3

Objective: The objective of this study was to investigate the regulation relationship of Teneleven translocation 1 (Tet1) in DNA demethylation and the proliferation of primordial germ cells (PGCs) in chickens. Methods: siRNA targeting Tet1 was used to transiently knockdown the expression of Tet1 in chicken PGCs, and the genomic DNA methylation status was measured. The proliferation of chicken PGCs was detected by flow cytometry analysis and cell counting kit-8 assay when activation or inhibition of Wnt4/β-catenin signaling pathway. And the level of DNA methylation and hisotne methylation was also tested. Results: Results revealed that knockdown of Tet1 inhibited the proliferation of chicken PGCs and downregulated the mRNA expression of Cyclin D1 and cyclin-dependent kinase 6 (CDK6), as well as pluripotency-associated genes (Nanog, PouV, and Sox2). Flow cytometry analysis confirmed that the population of PGCs in Tet1 knockdown group displayed a significant decrease in the proportion of S and G2 phase cells, which meant that there were less PGCs entered the mitosis process than that of control. Furthermore, Tet1 knockdown delayed the entrance to G1/S phase and this inhibition was rescued by treated with BIO. Consistent with these findings, Wnt/β-catenin signaling was inactivated in Tet1 knockdown PGCs, leading to aberrant proliferation. Further analysis showed that the methylation of the whole genome increased significantly after Tet1 downregulation, while hydroxymethylation obviously declined. Meanwhile, the level of H3K27me3 was upregulated and H3K9me2 was downregulated in Tet1 knockdown PGCs, which was achieved by regulating Wnt/β-catenin signaling pathway. Conclusion: These results suggested that the self-renewal of chicken PGCs and the maintenance of their characteristics were regulated by Tet1 mediating DNA demethylation through the activation of Wnt4/β-catenin signaling pathway.

Imaging of Anal Fistulas: Comparison of Computed Tomographic Fistulography and Magnetic Resonance Imaging

Changhu Liang,Yongchao Lu,Bin Zhao,Yinglin Du,Cuiyan Wang,Wanli Jiang 대한영상의학회 2014 Korean Journal of Radiology Vol.15 No.6

The primary importance of magnetic resonance (MR) imaging in evaluating anal fistulas lies in its ability to demonstrate hidden areas of sepsis and secondary extensions in patients with fistula in ano. MR imaging is relatively expensive, so there are many healthcare systems worldwide where access to MR imaging remains restricted. Until recently, computed tomography (CT) has played a limited role in imaging fistula in ano, largely owing to its poor resolution of soft tissue. In this article, the different imaging features of the CT and MRI are compared to demonstrate the relative accuracy of CT fistulography for the preoperative assessment of fistula in ano. CT fistulography and MR imaging have their own advantages for preoperative evaluation of perianal fistula, and can be applied to complement one another when necessary.

Identification of relevant differential genes to the divergent development of pectoral muscle in ducks by transcriptomic analysis

Li Fan,He Zongliang,Lu Yinglin,Zhou Jing,Cao Heng,Zhang Xingyu,Ji Hongjie,Lv Kunpeng,Yu Debing,Yu Minli 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.8

Objective: The objective of this study was to identify candidate genes that play important roles in skeletal muscle development in ducks.Methods: In this study, we investigated the transcriptional sequencing of embryonic pectoral muscles from two specialized lines: Liancheng white ducks (female) and Cherry valley ducks (male) hybrid Line A (LCA) and Line C (LCC) ducks. In addition, prediction of target genes for the differentially expressed mRNAs was conducted and the enriched gene ontology (GO) terms and Kyoto encyclopedia of genes and genomes signaling pathways were further analyzed. Finally, a protein-to-protein interaction network was analyzed by using the target genes to gain insights into their potential functional association.Results: A total of 1,428 differentially expressed genes (DEGs) with 762 being up-regulated genes and 666 being down-regulated genes in pectoral muscle of LCA and LCC ducks identified by RNA-seq (p<0.05). Meanwhile, 23 GO terms in the down-regulated genes and 75 GO terms in up-regulated genes were significantly enriched (p<0.05). Furthermore, the top 5 most enriched pathways were ECM-receptor interaction, fatty acid degradation, pyruvate degradation, PPAR signaling pathway, and glycolysis/gluconeogenesis. Finally, the candidate genes including integrin b3 (Itgb3), pyruvate kinase M1/2 (Pkm), insulinlike growth factor 1 (Igf1), glucose-6-phosphate isomerase (Gpi), GABA type A receptorassociated protein-like 1 (Gabarapl1), and thyroid hormone receptor beta (Thrb) showed the most expression difference, and then were selected to verification by quantitative realtime polymerase chain reaction (qRT-PCR). The result of qRT-PCR was consistent with that of transcriptome sequencing.Conclusion: This study provided information of molecular mechanisms underlying the developmental differences in skeletal muscles between specialized duck lines. Objective: The objective of this study was to identify candidate genes that play important roles in skeletal muscle development in ducks. Methods: In this study, we investigated the transcriptional sequencing of embryonic pectoral muscles from two specialized lines: Liancheng white ducks (female) and Cherry valley ducks (male) hybrid Line A (LCA) and Line C (LCC) ducks. In addition, prediction of target genes for the differentially expressed mRNAs was conducted and the enriched gene ontology (GO) terms and Kyoto encyclopedia of genes and genomes signaling pathways were further analyzed. Finally, a protein-to-protein interaction network was analyzed by using the target genes to gain insights into their potential functional association. Results: A total of 1,428 differentially expressed genes (DEGs) with 762 being up-regulated genes and 666 being down-regulated genes in pectoral muscle of LCA and LCC ducks identified by RNA-seq (p<0.05). Meanwhile, 23 GO terms in the down-regulated genes and 75 GO terms in up-regulated genes were significantly enriched (p<0.05). Furthermore, the top 5 most enriched pathways were ECM-receptor interaction, fatty acid degradation, pyruvate degradation, PPAR signaling pathway, and glycolysis/gluconeogenesis. Finally, the candidate genes including integrin b3 (Itgb3), pyruvate kinase M1/2 (Pkm), insulinlike growth factor 1 (Igf1), glucose-6-phosphate isomerase (Gpi), GABA type A receptorassociated protein-like 1 (Gabarapl1), and thyroid hormone receptor beta (Thrb) showed the most expression difference, and then were selected to verification by quantitative realtime polymerase chain reaction (qRT-PCR). The result of qRT-PCR was consistent with that of transcriptome sequencing. Conclusion: This study provided information of molecular mechanisms underlying the developmental differences in skeletal muscles between specialized duck lines.

Transcriptome characterization and profiling related to detoxification enzyme genes in Chilo sacchariphagus (Lepidoptera Crambidae)

Liu Jianbai,Yi Jiequn,Wu Han,Lu Yinglin,Mao Yongkai,Lin Mingjiang,Li Jihu,An Yuxing 한국곤충학회 2022 Entomological Research Vol.52 No.9

Chilo sacchariphagus Bojeris is one of the most dangerous pests of sugarcane. The larvae damage the seedlings and stems of sugarcane and also harm sorghum, corn and other crops, which causes great economic losses to the sugar industry every year. Transcriptome sequencing and expression profile analysis of cytochrome P450 monooxygenase (CYP), carboxylesterase and glutathione S-transferase (GST) were carried out, which could provide a basis for drug resistance monitoring and drug resistance management of the pest. Unigenes of C. sacchariphagus were obtained by using the Illumina HiSeqTM 4,000 platform as 150 bp paired-end reads. A total of 173,013 unigenes were obtained after data assembly and redundancy removal. 28,330 unigenes were annotated based on multiple public databases, and the number of unigenes annotated by NR database was the largest. According to the transcriptome analysis, 214 candidate detoxification enzyme genes were identified, including 44 GSTs,138CYPs, and 32 CarEs. Phylogenetic analysis showed that the CYPs were mainly clustered in CYP4, CYP6, CYP9 and CYP12 subfamilies; CarEs mainly include antennal CarEs, venom CarEs and CarEs NOTUM; while the GSTs cluster mainly contains subfamilies such as delta, omega, epsilon, theta, zeta and sigma. In this study, transcriptome information of C. sacchariphagus was obtained, and genes related to detoxification were identified, which could provide data and a basis for the further study of detoxification and host plant adaptation mechanism of C. sacchariphagus.