A Novel Facile and Green Synthesis Protocol to Prepare High Strength Regenerated Silk Fibroin/SiO2 Composite Fiber

Qiang Liu,Zhaohui Meng,Ronghui Wu,Liyun Ma,Wu Qiu,Honghao Zhang,Shuihong Zhu,Lingqing Kong,Zijjie Xu,Aniruddha Patil,Xiangyang Liu 한국섬유공학회 2019 Fibers and polymers Vol.20 No.10

In this work, regenerated silk fibroin (RSF) and silicon dioxide (SiO2) composite fiber was successfully extrudedby wet spinning method. The effect of SiO2 addition on structure of the composite fiber at microscopic level is studied, whichsubsequently correlated to the mechanical performance. The best concentration ratio for composite fiber is identified byscreening SiO2 concentration from 0.025 w/w% to 0.5 w/w%. The experimental results revealed that the SiO2 at a lowconcentration of 0.1 w/w% was well distributed. The breaking stress, breaking strain and Young’s modulus at 0.1 w/w% SiO2addition of the RSF fibers increased considerably compared to the neat RSF fibers from 243±3 to 458±21 MPa, 51±4 % to54±7 % and 6.34±0.55 to 11.69±1.12 GPa, respectively. To the best of our knowledge, this is the first report for RSF/SiO2composite fiber. We believed the insight provided in this report which looks into the structural evolution should be beneficialto the future design and building of other advanced functional fibers.

SPINK1 promotes cell growth and metastasis of lung adenocarcinoma and acts as a novel prognostic biomarker

( Liyun Xu ),( Changchang Lu ),( Yanyan Huang ),( Jihang Zhou ),( Xincheng Wang ),( Chaowu Liu ),( Jun Chen ),( Hanbo Le ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.12

Serine protease inhibitor Kazal type 1 (SPINK1) plays a role in protecting the pancreas against premature activation of trypsinogen and is involved in cancer progression. SPINK1 promoted LAC cells growth, migration, and invasion. Mechanistically, we found that SPINK1 promoted LAC cells migration and invasion via up-regulating matrix metalloproteinase 12 (MMP12). We observed that SPINK1 expression was only up-regulated in lung adenocarcinoma (LAC) tissues, and was an independent prognostic factor for poor survival. Our results indicate that SPINK1 might be a potential biomarker for LAC that promotes progression by MMP12. [BMB Reports 2018; 51(12): 648-653]

Effect on the Photocatalytic Activity of TiO2NTs Under Visible Light of Synergistic Effect of Ti3+ and S

Liyun Xiang,Jing Ya,Lixia Li,Zhifeng Liu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.2

TiO2 nanotube arrays (TiO2NTs) were prepared by anodic oxidation, then soaked in thiourea solution, and finally transformed into modified anatase TiO2 structure by heat treatment. Through the test results of SEM, TEM, XRD, XPS, UV-vis diffuse reflectance spectroscopy, photo-current and so on, finding that S replaces Ti4+ in the form of S6+, and part of the Ti4+ is reduced to Ti3+. Ladder type energy band structure, producing by the appearance of S6+ and Ti3+, makes the electron–hole more easily and increases the carrier transfer speed to a certain degree. The photo-current size has increased from 1.07 mA/cm2 to 2.13 mA/cm2. By simulating decolorization test of sample to methylthionine chloride under visible light, the degradation rate of the modified sample to methylene blue increases from 32% to 63%, and increased by 1.97 times.

Dynamic performances analysis and optimization of novel high-speed electromagnetic actuator for electronic fuel injection system of diesel engine

Peng Liu,Liyun Fan,Wei Zhou,Xiuzhen Ma,Enzhe Song 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.8

High-speed electromagnetic actuator (HEA) is the key component of Electronic fuel injection system (EFIS) of diesel engine, its dynamic performances have a significant impact on the control accuracy and flexible control law of fuel injection. In order to obtain the higher dynamic response speed, lower power consumption, higher safety and reliability, a novel HEA with permanent magnet (NPMHEA) was proposed based on the principle of parallel magnetic circuit in this paper. Then the simulation models of HEA coupling of magnetic field, electric circuit and mechanical motion were developed by the method of time-stepping finite element, and their precisions were verified by experiment. By the comparative analysis, influence rules of key parameters on the dynamic performances of HEA have been established. In addition, the multi-objective optimization of NPMHEA based on response surface methodology and Genetic algorithm (GA) was carried out and the Pareto optimal solution set was obtained. It is concluded that there are contradictions between objectives for the Pareto optimal solutions; considering the whole performances, the optimal solution is determined for which the peak current, the peak pulse and the hold current reduce by 20.5 %, 7.8 % and 43.9 %, respectively, and the actuation response speed quickens by 11.9 %, significantly reducing the power consumption and coil loss and improving the dynamic response speed of HEA. It decisively provides certain theoretical guidance for the design and optimization of HEA.

A Dynamic Placement Mechanism of Service Function Chaining Based on Software-defined Networking

( Yicen Liu ),( Yu Lu ),( Xingkai Chen ),( Xi Li ),( Wenxin Qiao ),( Liyun Chen ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.10

To cope with the explosive growth of Internet services, Service Function Chaining (SFC) based on Software-defined Networking (SDN) is an emerging and promising technology that has been suggested to meet this challenge. Determining the placement of Virtual Network Functions (VNFs) and routing paths that optimize the network utilization and resource consumption is a challenging problem, particularly without violating service level agreements (SLAs). This problem is called the optimal SFC placement problem and an Integer Linear Programming (ILP) formulation is provided. A greedy heuristic solution is also provided based on an improved two-step mapping algorithm. The obtained experimental results show that the proposed algorithm can automatically place VNFs at the optimal locations and find the optimal routing paths for each online request. This algorithm can increase the average request acceptance rate by about 17.6% and provide more than 20-fold reduction of the computational complexity compared to the Greedy algorithm. The feasibility of this approach is demonstrated via NetFPGA-10G prototype implementation.

Imbalance of Innate and Adaptive Immunity in Esophageal Achalasia

Lu Yao,Zuqiang Liu,Weifeng Chen,Jiaqi Xu,Xiaoyue Xu,Jiaxin Xu,Liyun Ma,Xiaoqing Li,Quanlin Li,Pinghong Zhou 대한소화기 기능성질환∙운동학회 2023 Journal of Neurogastroenterology and Motility (JNM Vol.29 No.4

Background/AimsPrevious studies reveal that immune-mediated neuroinflammation plays a key role in the etiology of esophageal achalasia. However, the understanding of leucocyte phenotype and proportion is limited. This study aim to evaluate the phenotypes of leukocytes and peripheral blood mononuclear cells transcriptomes in esophageal achalasia. MethodsWe performed high-dimensional flow cytometry to identified subsets of peripheral leukocytes, and further validated in lower esophageal sphincter histologically. RNA sequencing was applied to investigate the transcriptional changes in peripheral blood mononuclear cells of patients with achalasia. Cell-type Identification by Estimating Relative Subsets of RNA Transcripts (CIBERSORT) was used for estimating the immune cell types. A differential gene expression analysis was performed and the differential expressed genes were subjected to gene ontology, Kyoto Encyclopedia of Genes and Genomes network, protein-protein interaction network construction. ResultsAn imbalance between innate and adaptive immune cells occurred in achalasia. Specifically, neutrophils and CD8+ T cells increased both in peripheral blood and lower esophageal sphincter in achalasia. Eosinophils decreased in peripheral blood but massively infiltrated in lower esophageal sphincter. CIBERSORT analysis of peripheral blood mononuclear cells RNA sequencing displayed an increased prevalence of CD8+ T cells. 170 dysregulated genes were identified in achalasia, which were enriched in immune cells migration, immune response, etc. Proton pump inhibitor analysis revealed the intersections and gained 7 hub genes in achalasia, which were IL-6, Toll-like receptor 2, IL-1β, tumor necrosis factor, complement C3, and complement C1q A chain. ConclusionPatients with achalasia exhibited an imbalance of systematic innate and adaptive immunity, which may play an important role in the development of achalasia.

Effect of TiBCN Content on Microstructure and Properties of Laser Cladding Ti/TiBCN Composite Coatings

Yuxin Li,Keqiang Su,Peikang Bai,Liyun Wu,Bin Liu,Hongwen Su,Wenbo Du 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.5

The Ti/TiBCN composite coatings were fabricated on the substrate of Ti6Al4V alloy by laser cladding. The effects of TiBCNcontent on the microstructre, microhardness, corrosion resistance and tribological properties of the composite coatings werestudied. The results showed that, when the TiBCN content was 60 wt%, the composite coatings were mainly composed ofthe dendritic and rod-shaped TiBCN phases, small white lump TiC phases, fine lamellar TiN phases, small and scatteredwhite block TiB2phases, In addition, there was a small quantity of short whisker Al3Tiphases and uniform layer blockTiAl phases. Furhtermore, the maximum microhardness was 1596 HV, which was about 4.6 folds greater than that of thesubstrate; the corrosion potential (Ecorr) reached − 1.258 V and the corrosion current density (Icorr) was 4.035 × 10−5 A/cm2,which was one order lower than that of the substrate (1.172 × 10−4 A/cm2); the worn surface was relatively smooth and themass loss (1.22 g) was only 9/50 of that of the substrate (6.71 g).

Description of Ornithinimicrobium cryptoxanthini sp. nov., a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments

Huang Yuyuan,Jiao Yifan,Zhang Sihui,Tao Yuanmeihui,Zhang Suping,Jin Dong,Pu Ji,Liu Liyun,Yang Jing,Lu Shan 한국미생물학회 2023 The journal of microbiology Vol.61 No.4

Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37′13″N, 96°05′37″E) in July 2019. Both strains were shown to grow at 15–35 °C and pH 7.0–10.0, and in the presence of 0–6.0% (w/v) NaCl. The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6–98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5–98.6%) and O. murale 01-Gi-040T (98.3–98.5%). The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0–23.9% and 70.8–80.4%, respectively, all below the respective recommended 70.0% and 95–96% cutoff point. Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9. Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids. β-cryptoxanthin ( C40H56O) can be extracted from strain JY.X270T, and its content is 6.3 μg/ml. Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp. nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).

Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces

Huang Yuyuan,Dong Lingzhi,Gong Jian,Yang Jing,Lu Shan,Lai Xin-He,Jin Dong,Huang Qianni,Pu Ji,Liu Liyun,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.10

Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20 54 N, 106°49 20 E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09 10 N, 102°04 39 E, October 2013) of South China, respectively. Optimal growth is obtained at 25–28°C (range, 4–32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0–10.0) in the presence of 0.5– 1.0% NaCl (w/v) (range, 0–15% NaCl [w/v]). The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7–46.8%; ANI, 91.9–92.1%). Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively. The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T. Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001. Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene. Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp. nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).

Isolation and characterization of tick-borne Roseomonas haemaphysalidis sp. nov. and rodent-borne Roseomonas marmotae sp. nov.

Zhu Wentao,Zhou Juan,Lu Shan,Yang Jing,Lai Xin-He,Jin Dong,Pu Ji,Huang Yuyuan,Liu Liyun,Li Zhenjun,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.2

Four novel Gram-negative, mesophilic, aerobic, motile, and cocci-shaped strains were isolated from tick samples (strains 546T and 573) and respiratory tracts of marmots (strains 1318T and 1311). The 16S rRNA gene sequencing revealed that strains 546T and 573 were 97.8% identical to Roseomonas wenyumeiae Z23T, whereas strains 1311 and 1318T were 98.3% identical to Roseomonas ludipueritiae DSM 14915T. In addition, a 98.0% identity was observed between strains 546T and 1318T. Phylogenetic and phylogenomic analyses revealed that strains 546T and 573 clustered with R. wenyumeiae Z23T, whereas strains 1311 and 1318T grouped with R. ludipueritiae DSM 14915T. The average nucleotide identity between our isolates and members of the genus Roseomonas was below 95%. The genomic G+C content of strains 546T and 1318T was 70.9% and 69.3%, respectively. Diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE) were the major polar lipids, with Q-10 as the predominant respiratory quinone. According to all genotypic, phenotypic, phylogenetic, and phylogenomic analyses, the four strains represent two novel species of the genus Roseomonas, for which the names Roseomonas haemaphysalidis sp. nov. and Roseomonas marmotae sp. nov. are proposed, with 546T (= GDMCC 1.1780T = JCM 34187T) and 1318T (= GDMCC 1.1781T = JCM 34188T) as type strains, respectively.