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Wang, Lan,Wang, Li,Li, Xiaojuan,Wang, Linsong,Roh, Kwang-Soo,Wang, Myeong-Hyeon The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.2
Light and temperature variations are important during the vegetative stage of the Chinese red radish. We found that peroxidase (POD) and catalase activities and the content of pelargonidin were decreased by treatments of short of light perioid and low temperatures, while the content of hydrogen peroxide was increased. The POD isoenzymes of the radish organs were detected diversely depending on the developmental stages by polyacrylamide gel electrophoresis analysis; semi-quantitative RT-PCR revealed that expression was significantly induced during the flourishing anthesis stage in the bud. These results suggest that POD expression is both time-dependent and tissue-specific and that light and temperature conditions can influence the growth and antioxidant activity of the radish.
Lan Wang,노광수,Li Wang,Xiaojuan Li,Linsong Wang,왕명현 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.2
Light and temperature variations are important during the vegetative stage of the Chinese red radish. We found that peroxidase (POD) and catalase activities and the content of pelargonidin were decreased by treatments of short of light perioid and low temperatures, while the content of hydrogen peroxide was increased. The POD isoenzymes of the radish organs were detected diversely depending on the developmental stages by polyacrylamide gel electrophoresis analysis; semi-quantitative RT-PCR revealed that expression was significantly induced during the flourishing anthesis stage in the bud. These results suggest that POD expression is both timedependent and tissue-specific and that light and temperature conditions can influence the growth and antioxidant activity of the radish.
Modulation of Endogenous Peroxidase by Exogenous Peroxidase in Chinese Red Radish Seedling
Li Wang,Lan Wang,Fang-Jun Li,Linsong Wang,왕명현 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.5
To investigate the peroxidase function and its signal pathway, activity and isozyme pattern of peroxidase and transcription level of the peroxidase gene rsprx1 and rsprx2 in Chinese red radish seedling (Raphanus sativus L.) were investigated after administration of the exogenous peroxidase (extract of radish). The activity and isozyme pattern of peroxidases were increased, among cotyledon, hypocotyl and root, the most significant increase of peroxidases activity was observed in hypocotyls, the activity of phenylalanine ammonialyase was also increased, but the pelargonidin content was decreased. Although the exogenous application of horseradish peroxidase and the extract of radish could both affect the activity of endogenous enzyme and metabolites of Chinese red radish seedlings, the latter was more effective than the former. Nifedipine, as L-calcium channel blocker, affected the activity of peroxidase and pelargonidin content in Chinese red radish seedling. Exogenously added nifedipine with the extract caused reversal of the effects. It is concluded that calcium was involved in the signal pathway of the extract of radish in Chinese red radish seedling.
Haotian Qi,Ping Wang,Linsong Jiang 한국항공우주학회 2022 International Journal of Aeronautical and Space Sc Vol.23 No.2
A numerical method based on Reynolds Averaged Navier–Stokes (RANS) equations and moving overset mesh technique is developed to simulate the unsteady flow field of a rigid coaxial rotor. A high-efficient hybrid trim model is adopted to ensure the simulation accuracy of lift-offset (LOS). Cases in different advance ratios are trimmed for constant thrust coefficient and torque-balance. The effects of LOS, rotor spacing and RPM on the aerodynamic performance and interaction are analyzed. Results show that, in forward flight the lift–drag ratio can be improved by appropriate LOS. The rotor drag increases with LOS, as there is also a corresponding offset of drag. The optimum LOS varies with flight speed. At large advance ratio the interactions of the coaxial rotor are much weak, except the blade–meeting interaction. The interaction is typically illustrated by the impulsive loads of the upper blade at retreating side (270°), as the flow field is dominated by the advancing blade (90°) of the lower rotor. The interaction intensity is sensitive to LOS, rotor spacing and RPM, as it depends on the flow field gradient induced by the lower blade acting on the upper blade.