Interaction of <i>Veratrum nigrum</i> with <i>Panax ginseng</i> against Obesity: A Sang-ban Relationship

Park, Jinbong,Jeon, Yong-Deok,Kim, Hye-Lin,Lim, Hara,Jung, Yunu,Youn, Dong-Hyun,Jeong, Mi-Young,Kim, Hyun-Ju,Kim, Sung-Hoon,Kim, Su-Jin,Hong, Seung-Heon,Um, Jae-Young Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>Obesity has become a major health threat in developed countries. However, current medications for obesity are limited because of their adverse effects. Interest in natural products for the treatment of obesity is thus rapidly growing. Korean Medicine (KM) is characterized by the wide use of herbal formulas. However, the combination rule of herbal formulas in KM lacks experimental evidence. According to <I>Shennong's Classic of Materia Medica</I>, the earliest book of herbal medicine, <I>Veratrum nigrum</I> (VN) has antagonistic features against <I>Panax ginseng</I> (PG), and the PG-VN pair is strictly forbidden. In this study, we have shown the effects of PG, VN, and their combination on obesity in high-fat (HF) diet-induced obese mice and in 3T3-L1 cells. PG, VN, and PG-VN combination significantly reduced weight gain and the fat pad weight in HF diet-induced obese mice. They also significantly decreased lipid accumulation and the expressions of two major adipogenesis factors, PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I>, in 3T3-L1 cells. In addition, the PG-VN combination had synergistic effects compared with the mixture of extracts of PG and VN on inhibition of PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I> expressions at lower doses. These results indicate a new potential anti-obese pharmacotherapy and also provide scientific evidence supporting the usage of herbal combinations instead of mixtures in KM.</P>

우리나라에서 솔껍질깍지벌레의 분포확산

임유진,이상명,김동수,김준범,최광식,이신혜,박정규,이동운,Lim, Eu-Gene,Lee, Sang-Myeong,Kim, Dong-Soo,Kim, Joon-Bum,Lee, Shin-Hye,Choi, Kwang-Sik,Park, Chung-Gyoo,Lee, Dong-Woon 한국응용곤충학회 2012 한국응용곤충학회지 Vol.51 No.1

솔껍질깍지벌레는 우리나라 곰솔림의 주요한 해충의 하나이다. 본 연구는 우리나라에서 솔껍질깍지벌레의 분포를 알아보기 위하여 7개도 3개 광역시, 91개 시군구, 686 읍면동의 해송림에서 2010년 밀도를 조사하였다. 5개도 2개 광역시에서 솔껍질깍지벌레가 서식하고 있었는데 시군구별로는 조사대상의 64.8%인 59개 시군구에서 서식이 확인되었다. 솔껍질깍지벌레의 분포는 남해안 전역과 동해안의 포항, 서해안의 충남 보령지역까지 분포하였다. 충북과 대전광역시 및 제주도에서는 솔껍질깍지벌레의 분포가 확인되지 않았다. 부산지역은 16곳의 모든 구에서 솔껍질깍지벌레가 분포하였고, 시군 단위에서는 전남 해남군의 솔껍질깍지벌레 평균밀도가 1.713마리/0.785 $cm^2$로 가장 높았으며 읍면동 단위에서는 해남군 송지면이 6.36마리/0.785 $cm^2$로 가장 높았다. 곰솔 가지 내의 솔껍질깍지벌레 밀도와 솔껍질깍지벌레가 서식하고 있는 빈도와는 높은 상관관계가 있었다(상관계수=0.89). The black pine bast scale, $Masucoccus$ $thunbergianae$ (Hemiptera: Margarodidae), is a serious pest of the Japanese black pine, $Pinus$ $thunbergii$, in Korea. The distribution of the black pine bast scale was examined, looking overall at 686 towns (eup), townships (myeon) or neighborhoods (dong). There were Japanese black pine ($Pinus$ $thunbergii$) forests in 91 cities, counties (gun) and borough (gu), in seven provinces and three metropolitan cities during 2010. Black pine bast scale were found in 64.8% of cities or counties or borough (59) in 7 provinces and 3 metropolitan cities, and were distributed in all South Costal regions, Pohang in East Costal region and Boryeong in West Costal region. Chungcheongbukdo, Daejeon and Jeju did not have black pine bast scale. All the gu regions in Busan had black pine bast scale, of which the area with the highest prevalence was Haenam in Jeollanamdo (1.713 crawlers/0.785 $cm^2$). Songji-myeon had the highest occurrence rate (6.36 crawlers/0.785 $cm^2$) from the towns, township and dong. The density of black pine bast scale in twigs was highly correlated with percentage of the sample with scale (Correlation coefficacy=0.89).

Heterogeneous Capillary Interactions of Interface-Trapped Ellipsoid Particles Using the Trap-Release Method

Lim, Jin Hyun,Kim, Jun Young,Kang, Dong Woo,Choi, Kyu Hwan,Lee, Seong Jae,Im, Sang Hyuk,Park, Bum Jun American Chemical Society 2018 Langmuir Vol.34 No.1

<P>Heterogeneous capillary interactions between ellipsoid particles at the oil–water interface were measured via optical laser tweezers. Two trapped particles were aligned in either tip-to-tip (<I>tt</I>) or side-to-side (<I>ss</I>) configurations via the double-trap method and were released from the optical traps, leading to particle–particle attractions due to the capillary forces caused by quadrupolar interface deformation. On the basis of image analysis and calculations of the Stokes drag force, the capillary interactions between two ellipsoid particles with the same aspect ratio (<I>E</I>) were found to vary with the particle pairs that were measured, indicating that the interactions were nondeterministic or heterogeneous. Heterogeneous capillary interactions could be attributed to undulation of the interface meniscus due to chemical and/or geometric particle heterogeneity. The power law exponent for the capillary interaction <I>U</I><SUB>cap</SUB> ≈ <I>r</I><SUP>–β</SUP> was found to be β ≈ 4 and was independent of the aspect ratio and particle configuration in long-range separations. Additionally, with regard to the <I>tt</I> configuration, the magnitude of the capillary force proportionally increased with the <I>E</I> value (<I>E</I> > 1) when two ellipsoid particles approached each other in the <I>tt</I> configuration.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2018/langd5.2018.34.issue-1/acs.langmuir.7b03882/production/images/medium/la-2017-03882p_0010.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/la7b03882'>ACS Electronic Supporting Info</A></P>

Mutualistic Consortia for the Stable Microbial Production of Value-added Chemicals from Carbon Monoxide

Dong Hwan KIM,Sang Hak CHA,Hyun Gyu LIM,Seok Mu KWON,Chae won KANG,Gyoo Yeol JUNG 한국생물공학회 2023 한국생물공학회 학술대회 Vol.2023 No.10

Increased mitochondrial dysfunction associated with autophagy and mitophagy in cerebrospinal fluid cells following SAH in patients with DCI

Dong Hyuk Youn,Sung Woo Han,Seung Hyuk Lim,Tae Yeon Kim,Bong Jun Kim,Youngmi Kim,Jin Pyeong Jeon 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Reduced of mitochondrial membrane potential (ΔΨm) in human cerebrospinal fluid (hCSF) was observed in subarachnoid hemorrhage (SAH) with delayed cerebral ischemia (DCI). However, the underlyin mechanism of mitochondrial dysfunction on subarachnoid hemorrhage (SAH) with delayed cerebral ischemia (DCI) has not been precisely elucidated yet. In this study, we examined the possibility of the mitochondrial dysfunction cause by autophagy and mitophagy in hCSF cells for possible insight into DCI pathogenesis. We collected CSF sample on 5 to 7 days from the 56 SAH patients (DCI, n=21; and non-DCI, n=35) treated with coil embolization. hCSF cells were analyzed using autophagy and mitophagy specific markers (DAPK1, BNIP3L, BAX, PINK1, ULK1, and NDP52) via qRT-PCR and western blotting of protein markers (pBECN1, BECN1, BNIP3L/NIX, DAPK1, PINK1, LC3, and P62). Compared with non-DCI, patients with DCI exhibited an significant induction of autophagic flux with accumulation of autophagic vacuoles, as increased mRNA expression level of degradated BECN1, LC3-II, and p62. mRNA expression (2-ΔCt) was significantly increased in DCI compared with non-DCI : DAPK1, 0.279 (0.220-0.297) in DCI vs. 0.043 (0.021-0.086) in non-DCI (p=0.001); BNIP3L, 0.134 (0.060-0.202) in DCI vs. 0.045 (0.020-0.101) in non- DCI (p=0.006); and PINK1, 0.064 (0.044–0.810) in DCI vs. 0.045 (0.012–0.063) in non-DCI (p=0.012). Confocal microscopy analysis was showed a colocalization with autophagy and mitophagy marker (DAPK1, BNIP3L/NIX, PINK1,and BECN1) for dysfunctional mitochondria in vWF-positive CSF cells. Further performed immunogold electron microscopy with anti- DAPK1 to confirm the localization of DAPK1 in CSF cells. Interestingly, DAPK1 was observed in damaged mitochondria. As a results, DCI pathogenesis was closely related to increase dysfunctional mitochondria caused by autophagy and mitophagy.

Kinetic experiments with in-situ Raman analysis on methane-ethane hydrate formation and dissociation in the presence of kinetic inhibitor

Sang Yeon Hong,Seong Deok Seo,Dong-Ha Lim,Hyeong Soon Moon,Young Cheol Lee,Ju Dong Lee 한국생산제조학회 2014 한국생산제조시스템학회 학술발표대회 논문집 Vol.2014 No.9

2.5D SLAM Algorithm with Novel Data Fusion Method Between 2D-Lidar and Camera

Sang Hyeon Oh,Kwak Dong Hwan,Hyun Tek Lim 제어로봇시스템학회 2023 제어로봇시스템학회 국제학술대회 논문집 Vol.2023 No.10

A two-step synthesis process of thermoelectric alloys for the separate control of carrier density and mobility

Lim, Sang-Soon,Kim, Byung Kyu,Kim, Seong Keun,Park, Hyung-Ho,Kim, Dong-Ik,Hyun, Dow-Bin,Kim, Jin-Sang,Baek, Seung-Hyub Elsevier 2017 JOURNAL OF ALLOYS AND COMPOUNDS Vol.727 No.-

<P><B>Abstract</B></P> <P>It is challenging to improve the thermoelectric figure-of-merit as its constituent terms such as Seebeck coefficient, electrical conductivity, and thermal conductivity, are inter-related in the way that the enhancement of one term leads to the degradation of others. Therefore, it is highly desirable to design a new synthesis process that allows us to independently control these terms. Here, we report a simple, two-step process combining spark plasma sintering (SPS) and post-annealing (PA) to separately control the carrier density and mobility in the p-type (Bi<SUB>0.2</SUB>Sb<SUB>0.8</SUB>)<SUB>2</SUB>Te<SUB>3</SUB>. High-temperature SPS enables enhancing the carrier mobility by reducing scattering sites such as grain boundaries. Then, the following PA at a lower temperature allows tailoring the carrier density without the degradation of mobility. Beyond bismuth telluride-based, room-temperature thermoelectric materials, we believe that our result will provide an insight for the performance enhancement of other thermoelectric materials such as oxide and skutterudite.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A two-step process is proposed to separately control the carrier density and the mobility. </LI> <LI> High-temperature spark plasma sintering is used to enhance the carrier mobility. </LI> <LI> The following low-temperature post-annealing can optimize the carrier density. </LI> </UL> </P>

Comparison of the Abbott RealTime High Risk HPV test and the Roche cobas 4800 HPV test using urine samples

Lim, Myong Cheol,Lee, Do-Hoon,Hwang, Sang-Hyun,Hwang, Na Rae,Lee, Bomyee,Shin, Hye Young,Jun, Jae Kwan,Yoo, Chong Woo,Lee, Dong Ock,Seo, Sang-Soo,Park, Sang-Yoon,Joo, Jungnam Elsevier 2017 JOURNAL OF VIROLOGICAL METHODS Vol.243 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Human papillomavirus (HPV) testing based on cervical samples is important for use in cervical cancer screening. However, cervical sampling is invasive. Therefore, non-invasive methods for detecting HPV, such as urine samples, are needed.</P> <P><B>Objectives</B></P> <P>For HPV detection in urine samples, two real-time PCR (RQ-PCR) tests, Roche cobas 4800 test (Roche_HPV; Roche Molecular Diagnostics) and Abbott RealTime High Risk HPV test (Abbott_HPV; Abbott Laboratories) were compared to standard cervical samples.</P> <P><B>Study design</B></P> <P>The performance of Roche_HPV and Abbott_HPV for HPV detection was evaluated at the National Cancer Center using 100 paired cervical and urine samples. The tests were also compared using urine samples stored at various temperatures and for a range of durations.</P> <P><B>Results</B></P> <P>The overall agreement between the Roche_HPV and Abbott_HPV tests using urine samples for any hrHPV type was substantial (86.0% with a kappa value of 0.7173), and that for HPV 16/18 was nearly perfect (99.0% with a kappa value of 0.9668). The relative sensitivities (based on cervical samples) for HPV 16/18 detection using Roche_HPV and Abbott_HPV with urine samples were 79.2% (95% CI; 57.9–92.9%) and 81.8% (95% CI; 59.7–94.8%), respectively. When the cut-off C<SUB>T</SUB> value for Abbott_HPV was extended to 40 for urine samples, the relative sensitivity of Abbott_HPV increased to 91.7% from 81.8% for HPV16/18 detection and to 87.0% from 68.5% for other hrHPV detection. The specificity was not affected by the change in the C<SUB>T</SUB> threshold.</P> <P><B>Conclusions</B></P> <P>Roche_HPV and Abbott_HPV showed high concordance. However, HPV DNA detection using urine samples was inferior to HPV DNA detection using cervical samples. Interestingly, when the cut-off C<SUB>T</SUB> value was set to 40, Abbott_HPV using urine samples showed high sensitivity and specificity, comparable to those obtained using cervical samples. Fully automated DNA extraction and detection systems, such as Roche_HPV and Abbott_HPV, could reduce the variability in HPV detection and accelerate the standardization of HPV detection in urine. Thus, urine samples may be an effective alternative for HPV detection in women who hesitate to participate in cervical cancer screening programs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The purpose of this study was to evaluate urine as a source of HPV detection. </LI> <LI> Two real-time PCR for HPV detection in urine showed high agreement. </LI> <LI> However, detection of HPV DNA using urine samples was inferior to HPV DNA detection using cervical samples. </LI> <LI> When the cut-off C<SUB>T</SUB> of Abbott RealTime High Risk HPV was adjusted to 40, urine samples were comparable to cervical samples. </LI> </UL> </P>

Development of an Efficient Microbial Process Using Non-edible Biomass by Engineering Vibrio sp. dhg

Sung Hwa WOO,Hyun Gyu LIM,Dong Hun KWAK,Yong Hee HAN,Sungwoo PARK,Sang Woo SEO,Gyoo Yeol JUNG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Most microorganisms including industrial host strains were not able to efficiently utilize non-preferred carbon sources contained in non-edible biomass, such as alginate, xylose, and arabinose. Therefore, choosing an appropriate host strain would determine the efficiency of the entire bioprocess. In this regard, due to a broad spectrum of sugar utilization and genetic tractability of Vibrio sp. dhg, it was studied as a platform strain for the processes using non-edible biomass as a feedstock; brown-macroalgae and lignocellulose in this study. In order to efficiently utilize brown macroalgae as a feedstock, Vibrio sp. dhg capable of catabolizing alginate was isolated. After genome sequencing, genetic engineering tools were established. The production strains for ethanol, 2,3-butanediol, and lycopene demonstrated unprecedent productivities from brown macroalgae-derived carbon sources (alginate and mannitol). In order to efficiently utilize lignocellulose as a feedstock, Vibrio sp. dhg was rationally and evolutionary engineered for improved xylose catabolism and removal of carbon catabolite repression (CCR). Lactate-producing strain was constructed based on previously engineered strain and showed high productivity from lignocellulose-derived sugars (glucose, arabinose, and xylose).