http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Wu, Bing-Li,Luo, Lie-Wei,Li, Chun-Quan,Xie, Jian-Jun,Du, Ze-Peng,Wu, Jian-Yi,Zhang, Pi-Xian,Xu, Li-Yan,Li, En-Min Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12
Background: Fascin, an actin-bundling protein forming actin bundles including filopodia and stress fibers, is overexpressed in multiple human epithelial cancers including esophageal squamous cell carcinoma (ESCC). Previously we conducted a microarray experiment to analyze fascin knockdown by RNAi in ESCC. Method: In this study, the differentially expressed genes from mRNA expression profilomg of fascin knockdown were analyzed by multiple bioinformatics methods for a comprehensive understanding of the role of fascin. Results: Gene Ontology enrichment found terms associated with cytoskeleton organization, including cell adhesion, actin filament binding and actin cytoskeleton, which might be related to fascin function. Except GO categories, the differentially expressed genes were annotated by 45 functional categories from the Functional Annotation Chart of DAVID. Subpathway analysis showed thirty-nine pathways were disturbed by the differentially expressed genes, providing more detailed information than traditional pathway enrichment analysis. Two subpathways derivated from regulation of the actin cytoskeleton were shown. Promoter analysis results indicated distinguishing sequence patterns and transcription factors in response to the co-expression of downregulated or upregulated differentially expressed genes. MNB1A, c-ETS, GATA2 and Prrx2 potentially regulate the transcription of the downregulated gene set, while Arnt-Ahr, ZNF42, Ubx and TCF11-MafG might co-regulate the upregulated genes. Conclusions: This multiple bioinformatic analysis helps provide a comprehensive understanding of the roles of fascin after its knockdown in ESCC.
Security Authentication Method of Terminal Trusted Access in Smart Grid
Yufeng Wang,Lie Wu,Yun Yang 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.7
A security authentication method in smart grid terminal is proposed in this paper. This method layers the terminal authentication system structure of smart grid so as to improve the simplicity and extensibility on system deployment and realize flexible communication mechanism and the interaction mechanism among systems. This paper aims at preventing terminal information from being destroyed and achieving terminal verification integrity. Besides, this kind of authentication method has no special requirements for terminal users.
Chow, Yvonne,Li, Ruijiang,Wu, Jinchuan,Puah, Sze Min,New, Shu Wei,Chia, Wei Qiang,Lie, Felicia,Rahman, Talukder Md Mahabubur,Choi, Won-Jae Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.4
The production of semi-synthetic beta-Iactam antibiotics such as Amoxicillin may be performed enzymatically using penicillin acylase under mild conditions. However, the thermodynamically favored hydrolysis of the antibiotic product and the acyl donor substrate needs to be minimized to use the kinetically controlled route. The addition of cosolvents such as ethylene glycol and methanol (the two best solvents identified so far for semi-synthetic beta-lactam antibiotics) can achieve this to some degree, but these additives also produce enzyme inhibition and deactivation. In this study, we compared ethylene glycol and methanol under various substrate conditions. Methanol gave a better synthesis to hydrolysis ratio, although its deactivating effects adversely affected production at lower cosolvent concentrations than ethylene glycol. This effect and its dependence on substrate concentration was further modeled and optimized. A few targets of optimization such as Amoxicillin level, the synthesis to hydrolysis ratio, or a combination, were employed. While maximum levels of Amoxicillin synthesis were achievable only at high substrate concentrations, improvements derived from cosolvents were most significant at low substrate concentrations.
Ming Yue,Xiangmin Wu,Lie Guo,Junjie Gao 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.10
In this paper, a dynamic automatic obstacle avoidance trajectory planning and tracking control framework is proposed for tractor-trailer system. Tractor-trailer is a special class of multibody and nonholonomic system, whose backward and forward operations have difference kinetic mechanisms. Because the obstacle avoidance behaviors are concerned with the two motion modes, the kinematic models including backward and forward movements are firstly derived. Secondly, a time-based quintic polynomial function is developed to plan two kinds of dynamic obstacle avoidance trajectories based on dynamics constraints and the information from on board sensors, so as to minimize the collision risk. Thirdly, a model predictive control (MPC)-based posture controller is designed, by which better tracking performance can be achieved for both forward and backward obstacle avoidance maneuvers. Lastly, the simulation results validate the effectiveness of the proposed dynamic obstacle avoidance framework and the designed methods.
Yvonne Chow,Ruijiang Li,Jinchuan Wu,Sze Min Puah,Shu Wei New,Wei Qiang Chia,Felicia Lie,Talukder Md Mahabubur Rahman,Won Jae Choi 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.4
The production of semi-synthetic beta-lactam antibiotics such as Amoxicillin may be performed enzymatically using penicillin acylase under mild conditions. However, the thermodynamically favored hydrolysis of the antibiotic product and the acyl donor substrate needs to be minimized to use the kinetically controlled route. The addition of cosolvents such as ethylene glycol and methanol (the two best solvents identified so far for semi-synthetic beta-lactam antibiotics) can achieve this to some degree, but these additives also produce enzyme inhibition and deactivation. In this study, we compared ethylene glycol and methanol under various substrate conditions. Methanol gave a better synthesis to hydrolysis ratio, although its deactivating effects adversely affected production at lower cosolvent concentrations than ethylene glycol. This effect and its dependence on substrate concentration was further modeled and optimized. A few targets of optimization such as Amoxicillin level, the synthesis to hydrolysis ratio, or a combination, were employed. While maximum levels of Amoxicillin synthesis were achievable only at high substrate concentrations, improvements derived from cosolvents were most significant at low substrate concentrations.
Chemical Constituents from the Aerial Parts of Isodon coetsa and their Cytotoxicity
Wei Zhao,Jian Xin Pu,Xue Du,Yong Zhao,Fei He,Hai Bo Zhang,Yong Bo Xue,Wei Lie Xiao,Han Dong Sun,Ying Li Wu,Guo Qiang Chen 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.12
Three new compounds (1-3), including a neolignan, a triterpenoid, and a diterpenoid, together with twenty known compounds (4-23), were isolated from the aerial parts of Isodon coetsa. Their structures and relative configurations were elucidated on the basis of spectroscopic data. Compounds 1, 3, 5-9, 11-13, 16-17, and 19-23 were evaluated for their cytotoxicity against HT-29, BEL-7402, and SK-OV-3 human tumor cell lines. Compound 7 showed significant inhibitory effects on all three types of cells, with IC50 values of 2.52, 3.06, 2.14 μM, respectively.