HCBP6 upregulates human SREBP1c expression by binding to C/EBPβ-binding site in the SREBP1c promoter

( Xueliang Yang ),( Ming Han ),( Shunai Liu ),( Xiaoxue Yuan ),( Xiaojing Liu ),( Shenghu Feng ),( Li Zhou ),( Yaru Li ),( Hongping Lu ),( Jun Cheng ),( Shumei Lin ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.1

Sterol regulatory element-binding protein-1c (SREBP1c) plays an important role in triglyceride (TG) homeostasis. Although our previous study showed that hepatitis C virus core-binding protein 6 (HCBP6) regulates SREBP1c expression to maintain intracellular TG homeostasis, the mechanism underlying this regulation is unclear. In the present study, we found that HCBP6 increased intracellular TG levels by upregulating SREBP1c expression. HCBP6 increased SREBP1c transcription by directly binding to the SREBP1c promoter (at the -139- to +359-bp region). Moreover, we observed that HCBP6 interacted with C/EBPβ-binding site in the SREBP1c promoter both in vitro and in vivo. These results indicate that HCBP6 upregulates human SREBP1c expression by binding to the C/EBPβ-binding site in the SREBP1c promoter. [BMB Reports 2018; 51(1): 33-38]

Design and optimization of novel transceiver device for one‑way single‑wire power transfer

Yang Li,Taocheng Hu,Xueliang Wang,Yujie Zhai,Yao Li,Wenxin Huang,Zhigang Lou 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.10

The further development of wireless power transfer technology is hindered by the limiting relationship between transmission distance, transmission efficiency, and size of the coupling mechanism. Thus, a feasible solution was provided by one-way single-wire power transfer. First, a horn-type mode conversion device was proposed in this study by analyzing the principle of one-way single-wire power transfer. Second, the system structure was designed to save materials and reduce the volume of space. The transmission efficiency of the system was improved, and the operating frequency was reduced by optimizing the parameters of the mode conversion device. Finally, the feasibility and structural applicability of the one-way single-wire power transfer method proposed in this study were proved by the experimental results.

Comments on the stability of the synchronous states of three vibrators in a vibrating system with two rigid frames

Xueliang Zhang,Zhihui Wang,Chao Li,Jinlin Xu 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.10

This paper focuses on the stability of three homodromy vibrators in a vibrating system with two rigid frames (RFs). The motion differential equations of the system are established. Using the average method yields the average coupling torque balanced equations of three vibrators, and the simplified analytical expressions for synchronization and stability criterions of the system were derived. The coupling dynamic characteristics were numerically analyzed, including frequency-amplitude response, stable phase differences, synchronization and stability ability, and phase relationships. The simulations to verify the validity of theoretical results were carried out. It is shown that the stable states of the system are classified into three types, thereof the stability of phase differences among vibrators is similar in sub-resonant and super-resonant states, which results in no vibration of the system. While in near sub-resonant state the system reflects strong useful positive superposition vibration with energy saving, which is exactly the desire in engineering design.

Preparation and Electrochemical Application of Porous Yttrium-Doped NiO Microspheres

Xueliang Jiang,Chenjian Li,Yanrong Gao,Canyu Yang,Feng You,Chu Yao 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.2

Using NiCl2 · 6H2O and Y(NO)3 solution as raw material and urea as the precipitating agent, Yttrium-doped NiO hollow spheres (Y-NiOHS) were prepared through homogeneous precipitation method with melamine–formaldehyde polymer microspheres (MF) as templates. The electrochemical performances of Y-NiOHS with difference Y-doped mole ratios (Y : Ni = 0.3%, 0.5%, 1% Y-NiOHS) were characterized by cyclic voltammetry (CV), constant current charge–discharge test (GCD) and AC impedance test (EIS). The results of CV show that 0.5% Y-NiOHS in 5 mV/s scan rate can achieve the maximum capacity of 320.92 F/g. The results of GCD show that the capacity of 0.5%Y-NiOHS is 226.2 F/g at 0.5 A/g discharge current density and the 1000 charge–discharge cycles after the capacitance retention rate is 85%. The electrochemical performance of 0.5%Y-NiOHS are better than NiOHS and NiO powder.

Optimized junction temperature fluctuation suppression technique for SiC MOSFETs in a wireless charging system

Ruoyin Wang,Xueliang Huang,Jiacheng Li 전력전자학회 2022 JOURNAL OF POWER ELECTRONICS Vol.22 No.5

The problem of SiC MOSFET junction temperature fluctuation in wireless charging systems must be addressed immediately. The existing temperature fluctuation suppression technique requires a large number of additional switches and capacitors. This study further optimizes the temperature fluctuation tracking suppression (TFTS) strategy. This method realizes closedloop temperature adjustment and greatly simplifies the system structure. In addition, an optimized TFTS (OTFTS) strategy combined with an optimized proportional–integral–derivative control method is proposed to solve integral saturation and the subsequent control instability phenomenon. Then, a 5.5 kW experimental system is built. Results show that the OTFTS strategy eliminates 17.9 °C junction temperature fluctuation on the basis of reducing the hardware cost. It also has a good dynamic response and junction temperature fluctuation suppression effect.

A sequential fuzzy diagnosis method for rotating machinery using ant colony optimization and possibility theory

Hao Sun,Ke Li,Huaqing Wang,Xueliang Ping,Yi Cao 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.4

This study proposes a novel intelligent fault diagnosis method for rotating machinery using ant colony optimization (ACO) and possibilitytheory. The non-dimensional symptom parameters (NSPs) in the frequency domain are defined to reflect the features of the vibrationsignals measured in each state. A sensitive evaluation method for selecting good symptom parameters using principal componentanalysis (PCA) is proposed for detecting and distinguishing faults in rotating machinery. By using ACO clustering algorithm, the synthesizingsymptom parameters (SSP) for condition diagnosis are obtained. A fuzzy diagnosis method using sequential inference and possibilitytheory is also proposed, by which the conditions of the machinery can be identified sequentially. Lastly, the proposed method iscompared with a conventional neural networks (NN) method. Practical examples of diagnosis for a V-belt driving equipment used in acentrifugal fan are provided to verify the effectiveness of the proposed method. The results verify that the faults that often occur in V-beltdriving equipment, such as a pulley defect state, a belt defect state and a belt looseness state, are effectively identified by the proposedmethod, while these faults are difficult to detect using conventional NN.

On bending, buckling and vibration of graphene nanosheets based on the nonlocal theory

Jinjian Liu,Ling Chen,Feng Xie,Xueliang Fan,Cheng Li 국제구조공학회 2016 Smart Structures and Systems, An International Jou Vol.17 No.2

The nonlocal static bending, buckling, free and forced vibrations of graphene nanosheets are examined based on the Kirchhoff plate theory and Taylor expansion approach. The nonlocal nanoplate model incorporates the length scale parameter which can capture the small scale effect. The governing equations are derived using Hamilton\'s principle and the Navier-type solution is developed for simply-supported graphene nanosheets. The analytical results are proposed for deflection, natural frequency, amplitude of forced vibration and buckling load. Moreover, the effects of nonlocal parameter, half wave number and three-dimensional sizes on the static, dynamic and stability responses of the graphene nanosheets are discussed. Some illustrative examples are also addressed to verify the present model, methodology and solution. The results show that the new nanoplate model produces larger deflection, smaller circular frequencies, amplitude and buckling load compared with the classical model.

Combinatorial Methylerythritol Phosphate Pathway Engineering and Process Optimization for Increased Menaquinone-7 Synthesis in Bacillus subtilis

Taichi Chen,Hongzhi Xia,Shixiu Cui,Xueqin Lv,Xueliang Li,Yanfeng Liu,Jianghua Li,Guocheng Du,Long Liu 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.5

Vitamin K2 (menaquinone) is an essential vitamin existing in the daily diet, and menaquinone-7 (MK- 7) is an important form of it. In a recent work, we engineered the synthesis modules of MK-7 in Bacillus subtilis, and the strain BS20 could produce 360 mg/l MK-7 in shake flasks, while the methylerythritol phosphate (MEP) pathway, which provides the precursor isopentenyl diphosphate for MK-7 synthesis, was not engineered. In this study, we overexpressed five genes of the MEP pathway in BS20 and finally obtained a strain (BS20DFHG) with MK-7 titer of 415 mg/l in shake flasks. Next, we optimized the fermentation process parameters (initial pH, temperature and aeration) in an 8-unit parallel bioreactor system consisting of 300-ml glass vessels. Based on this, we scaled up the MK-7 production by the strain BS20DFHG in a 50-l bioreactor, and the highest MK-7 titer reached 242 mg/l. Here, we show that the engineered strain BS20DFHG may be used for the industrial production of MK-7 in the future.

High-Throughput Sequencing Reveals Bacterial Diversity in Raw Milk Production Environment and Production Chain in Tangshan City of China

Huihui Cao,Yanhua Yan,Lei Wang,Lixue Dong,Xueliang Pang,Sining Tang,Aijun Li,Aili Xiang,Litian Zhang,Baiqin Zheng 한국축산식품학회 2021 한국축산식품학회지 Vol.41 No.3

Raw milk is a nature media of microbiota that access milk from various sources, which constitutes a challenge in dairy production. This study characterizes the relationship between the raw milk quality and the bacteria diversity at different sampling sites in dairy farms, aiming to provide a strong scientific basis for good hygienic practices and optimized procedure in milk production. High-throughput sequencing of 16S rRNA V3-V4 region was used to analyze the components, abundance and diversity of 48 bacterial population sampled from 8 different sites in dairy farm: pre-sterilized cow’s teats (C1), post-sterilized cow’s teats (C2), milking cluster (E), milk in storage tank (M1), transport vehicle (M2), storage equipment (E2), cow’s dung samples (F) and drinking water (W). Firmicutes account for predominantly 32.36% (C1), 44.62% (C2), 44.71% (E), 41.10% (M1), 45.08% (M2), 53.38% (F) of all annotated phyla. Proteobacteria accounts for 81.79% in W group and Actinobacteria 56.43% in E2 group. At the genus level, Acinetobacter was the most abundant genus that causes bovine mastitis, Acinetobacter and Arthrobacter were dominant in C1, C2, and E groups, Kocuria in E2 group and Arcobacter in W group. E, C1, and C2 group have very similar bacterial composition, and M1 and M2 demonstrated similar composition, indicating that the milking cluster was polluted by the environment or contact with cow udders. Bacterial population composition in different sampling sites identified by NGS reveals a correlation between the bacteria communities of raw milk production chain and the quality of raw milk.

Correlation analysis and prognostic value of miR-29a-3p expression and CYP2C19 genotypes in exfoliated cells from tongue coating of patients with gastroesophageal reflux disease

Zhang Aiqing,Liu Wenjuan,Niu Lingyun,Zhu Liping,Yang Liuqing,Li Xueliang 한국유전학회 2023 Genes & Genomics Vol.45 No.5

Background Gastroesophageal reflux disease (GERD) is a highly prevalent and troublesome disease. Several differentially expressed microRNAs (miRNAs) have been found in GERD. Objective This study was to analyze the correlation of miR-29a-3p expression and CYP2C19 genotypes in exfoliated cells from tongue coating of GERD patients and its prognostic value. Methods Tongue coating specimens were collected from 130 GERD patients and 70 healthy volunteers and their clinical baseline information was recorded. miR-29a-3p expression in exfoliated cells from tongue coating was determined via RT-qPCR, and its diagnostic efficiency on GERD was evaluated via receiver operating characteristic curve. CYP2C19 genotypes and their correlation with miR-29a-3p were analyzed via polymerase chain reaction restriction fragment length polymorphism technique. The adverse events of patients were documented via 12-month follow-up. The impact of miR-29a-3p expression on the healing rate of patients was analyzed via Kaplan-Meier method. Qualification of miR-29a-3p as an independent prognostic factor of GERD patients was analyzed via multivariate Cox regression analysis. Results miR-29a-3p was highly-expressed in exfoliated cells from tongue coating of GERD patients. miR-29a-3p expression had high specificity and sensitivity in diagnosing GERD. CYP2C19 genotypes in GERD patients comprised rapid metabolizers, intermedia metabolizers, and poor metabolizers. miR-29a-3p expression showed a correlation with CYP2C19 genotypes. Higher miR-29a-3p expression predicted higher cumulative incidences of adverse outcomes. Highly-expressed miR-29a-3p was an independent prognostic factor for adverse outcomes of GERD patients. Conclusion High expression of miR-29a-3p aided the diagnosis and predicted poor prognosis of GERD patients.