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Phytochemical constituents from the aerial parts of Salvia plebeia
Leo Adrianne Paje,Hak-Dong Lee,Jungwon Choi,Juree Kim,Ki Hyun Kim,A Ram Yu,Min-Jung Bae,Sanghyun Lee 한국응용생명화학회 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.4
Four compounds were isolated from Salvia plebeia aerial parts. Silica gel open column chromatography with a gradient elution system was used to isolate and purify these compounds. Nuclear magnetic resonance spectroscopy and mass spectroscopy were used for structural elucidation and identification, while electronic circular dichroism was used to confirm the absolute configuration. The structures were determined to be β- sitosterol (1), (–)-1S,5S,8S,10R-1-acetoxy-8-hydroxy-2-oxoeudesman- 3,7(11)-dien-8,12-olide (2), ursolic acid (3), and N-methylhydroxylamine (4). Compounds 2 and 4 were isolated for the first time from this plant. Compound 2 was quantitatively analyzed via HPLC/UV. The results showed that the methanol extract of S. plebeia had a higher content of compound 2 (1.20 mg/g) than the ethanol extract (0.55 mg/g). This study could be used as a preliminary step in conducting HPLC/UV analysis of sesquiterpenoids in S. plebeia extract to assess their bioavailability and potency.
Quantitative analysis of cordycepin in Cordyceps militaris under different extraction methods
( Jungwon Choi ),( Leo Adrianne Paje ),( Baekjun Kwon ),( Jaekyu Noh ),( Sanghyun Lee ) 한국응용생명화학회(구 한국농화학회) 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.2
Cordyceps militaris (CM) is one of the most important medicinal mushrooms known to possess various biological activities. Cordycepin (CP) is a bioactive compound present in the fruiting bodies of CM and is known to have anti-tumor, anti-metastatic immunomodulatory and anti-inflammatory activities. In this study, we aim to analyze CP quantitatively under various CM extraction conditions. CP was measured using high-performance liquid chromatography, quantified using a reversed phase column using a gradient elution system of water and acetonitrile, and detected with a UV absorbance wavelength of 260 nm. The CP content of CM was the highest in 100% ethanol extract of the fruiting bodies and 60% ethanol extract of the mycelium. This study provides an efficient analysis method to determine the optimal extraction conditions for CP that can be used as a basis for developing functional foods and pharmaceutical products derived from CM.
Determination of isovitexin from Lespedeza cuneata using a validated HPLC-UV method
( Ju Sung Lee ),( Leo Adrianne Paje ),( Sang-woo Yoo ),( Seong Lee ),( Ja-jung Ku ),( Sanghyun Lee ) 한국응용생명화학회(구 한국농화학회) 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.1
Isovitexin, a marker compound with various pharmacological activities, in Lespedeza cuneata, was analyzed using high performance liquid chromatography coupled with UV (HPLC/UV). There are no previous reports on using L. cuneata as the source material for the quantification of isovitexin. In this study, we developed an optimized method using HPLC-UV analysis, which was validated using various parameters. Our method demonstrated high specificity, and good separation of the chromatographic peak was achieved. Parameters such as linearity (r2>0.9997), precision, and accuracy indicated that our proposed analytical method had good reliability and sensitivity. These results demonstrate the utility and convenience of our method for rapidly quantifying isovitexin in L. cuneata extracts.
Lee Hak-Dong,Paje Leo Adrianne,Lee Sullim,Kang Ki Sung,Hong Kyungki,Kwon Hyukjin,Lee Sanghyun 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.S
An analytical method was established to identify and quantify hydroxycinnamic acids, such as 1,5-dicaffeoylquinic acid (DCQA) and chicoric acid (CA), in mixtures of Saussurea grandifolia and Taraxacum coreanum (MST) by using reverse-phase high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). Analyses were carried out by using an INNO C18 column with a gradient elution system, and different parameters were used to validate our optimized method. Results demonstrated limits of detection and quantification of 5.46 × 10– 3 and 16.54 × 10– 3 mg/mL for DCQA and 0.37 × 10– 3 and 1.14 × 10– 3 mg/mL for CA, respectively. The calibration curves for DCQA and CA showed good linearity over the concentration ranges of 0.025–0.4 and 0.00625–0.1 mg/mL, respectively, and both exhibited r2 = 1.0000. In the accuracy test, high recovery rates were obtained ranging from 101.16–104.18% for DCQA and 97.55–108.49% for CA, while the precision values were ≤ 1.00% for DCQA and ≤ 1.21% for CA. The values obtained from our analyses support the use of this analytical method for the accurate identification and quantification of DCQA and CA from MST. Our methodology could be used further to determine the content of hydroxycinnamic acid derivatives in routine analyses and large-scale extraction processes.
Validation of an optimized HPLC/UV method for the quantification of flavonoids in lotus
Lee Ju Sung,Paje Leo Adrianne,Choi Won-Hee,Cho Eun Ju,Kim Hyun Young,Jacinto Sonia D.,Lee Sanghyun 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.6
Flavonoids present in the leaves of lotus (Nelumbo nucifera) grown in diferent regions of South Korea (Yeongcheon, Haenam, and Seocheon) and at diferent harvest times (July to September) were determined. Flavonoid contents in lotus extracts were identifed and analyzed using high-performance liquid chromatography (HPLC). The HPLC results revealed that the favonoid contents of the lotus extracts varied at diferent harvesting times, with the highest content in July. Analysis of the favonoid content in the leaves from the diferent regions showed the highest contents of isorhamnetin-3-O-glucoside, quercetin 3-O-glucuronide, and quercetin 3-O-glucoside in Yeongcheon, Korea, and highest contnts of rutin, myricetin, kaempferol 3-O-glucoside, and quercetin in Haenam, Korea. The HPLC method was validated and optimized to quantify quercetin 3-O-glucuronide; it showed good linearity (1000–62.5 µg/mL, r 2=0.9999), accuracy (106%–108%), and precision (RSD≤1.70%). Determination of favonoid content in lotus is valu‑ able for producing medicinal crops and identifying the optimal sources to increase the quantity of clinically available medicines.
( Jun Yeon Park ),( Leo Adrianne Paje ),( Ki Sung Kang ),( Sanghyun Lee ) 한국응용생명화학회 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.3
Salicornia herbacea is a type of salt marsh plant that has been used in traditional medicine to treat several diseases. Isorhamnetin-3-O-glucoside (I3G) and quercetin-3-O-glucoside (Q3G) are major flavonoids in S. herbacea that are known to exert various pharmacological activities. Therefore, our study sought to validate and optimize an HPLC/UV-based analytical method for I3G and Q3G yield quantification, as well as to determine its limit of detection, limit of quantification, linearity, precision, and accuracy. Upon testing a concentration range of 31.5-1.9 μg/mL the results exhibited good linearity (r2 ≥0.9996 and r2 ≥0.9999 for I3G and Q3G, respectively), and the procedure was deemed precise (relative standard deviation of ≤3.19 and ≤3.85%, respectively), and accurate (102.6-105.0 and 92.9-95.2%, respectively). The results showed that our proposed method could be used for rapid I3G and Q3G evaluation in S. herbacea.
Lee Ju Sung,Paje Leo Adrianne,Kim Min Jeong,Jang Seung Hee,Kim Jong Tae,Lee Sanghyun 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.S
Here, two important isoflavones present in Trifolium pratense, formononetin and biochanin A, were analyzed by high performance liquid chromatography coupled with UV (HPLC–UV). These isoflavones are marker compounds with anti-inflammatory, anti-cancer, and anti-oxidant properties, and are also potent phytoestrogens that can be used in hormonal therapies. Till date, no study regarding rapid identification and quantification of T. pratense extract has been conducted. This study developed and validated an optimized method for quantifying formononetin and biochanin A using HPLC–UV. The results showed excellent linearity of the calibration curve ( r2 ≥ 0.999), and good resolutions of chromatographic peaks were obtained. Other validation parameters such as specificity, accuracy, and precision demonstrated that our method had good reliability and sensitivity. Furthermore, our method for quantifying formononetin and biochanin A in T. pratense extract is convenient.
Development of an Analytical Approach for the Utilization of Edible Tree Sprouts
Jungwon Choi,김주리,이학동,조혜진,Leo Adrianne Paje,Hanna Shin,이상현 한국생약학회 2022 Natural Product Sciences Vol.28 No.1
This study evaluated the general nutritional ingredients such as crude fats, crude ashes, crude proteins, total polyphenols, and total flavonoids in 18 kinds of edible tree sprouts. The tree sprouts of Philadelphus schrenckii, Lycium chinense, and Morus alba had the highest crude fat, crude ash, and crude protein content, respectively. The tree sprouts of Cedrela sinensis (CSS) with high ABTS+ radical scavenging activities had a high content of total polyphenols (175.65 mg/g ext.) and total flavonoids (75.18 mg/g ext.). The simultaneous determination of flavonoids such as rutin, isoquercitrin, quercitrin, afzelin, and quercetin in CSS was conducted using high-performance liquid chromatography with a wavelength of 270 nm. Among the flavonoids, the content of quercitrin in CSS was the highest at 59.28 mg/g ext. This study also aids the quality control of many edible tree sprouts by analyzing the general components, total polyphenols, and total flavonoids.