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Leesun Kim,Hyun Ho Noh,Chang Jo Kim,RaeKeun Lee,Xiu Yuan,Min Kim,Hee Jeong Shin,Danbi Kim,Hee-Dong Lee,Kee Sung Kyung 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.11
Traditionally in Korea, Protaetia brevitarsis seulensis (white spotted flower rainbow snail) has been used as a traditional medicine, and recently has attracted increased attention due to its antithrombotic efficacy. Spent mushroom compost is a feedstock for P. brevitarsis that may contain three fungicides, carbendazim, dimethomorph, and fenoxanil, which could be transferred to the edible insect. In this study, the analytical method for three fungicides in the edible insect was optimized using a simple extraction method combined with liquid chromatography tandem mass spectrometry. After the pulverized samples (5g) were extracted with acetonitrile (10mL) and formic acid (100 μL), fat and lipids in the samples were slowly precipitated at -20oC for 24 hours. After eight different clean-up methods were investigated, a dispersive solid-phase extraction tube containing 150 mg MgSO₄/25mg PSA/25mg C18 was selected based on optimal recovery of the target compounds. Recovery of carbendazim, dimethomorph, and fenoxanil was 77.9%-80.8% ± relative standard deviation (RSD) 0.8%-4.0%, 111.2%-116.7% ± RSD 2.8%-3.0%, and 111.9%-112.5% ± RSD 2.5%-5.2%, respectively. The simplified analytical method developed in this study can provide important data for establishing that the food meets pesticide safety standards and may be used for screening pesticides in P. brevitarsis before use in medical applications.
LC-MS/MS를 이용한 갈색거저리 유충 중 Fenoxanil, Thiobencarb 및 Fludioxonil의 잔류분석
김이선 ( Leesun Kim ),백수진 ( Sujin Baek ),손경애 ( Kyungae Son ),노현호 ( Hyunho Noh ),문병철 ( Byeong-chul Moon ),김단비 ( Danbi Kim ),오민석 ( Min-seok Oh ),노진호 ( Jin-ho Ro ),김은선 ( Eunsun Kim ) 한국환경농학회 2020 한국환경농학회 학술대회집 Vol.2020 No.-
This study was designed to determine fenoxanil, thiobencarb, and fludioxonil residues in mealworms, the larvae of Tenebrio moliter using liquid chromatography tandem mass spectrometry. These pesticides are officially applied for wheat cultivation, so can be found in wheat bran, recommended feed for mealworms in South Korea. In this regard, effective analytical method is urgently required because mealworms are used as food or feed as alternative protein sources. Acetonitrile (10 mL) was used to extract the target pesticides. Four clean-up procedures ((1) MgSO<sub>4</sub>+25 mg PSA+25 mg C18 (2) MgSO<sub>4</sub>+50 mg PSA+50 mg C18 (3) EMR-lipid™ tube, and (4) 10 mL n-hexane) were examined. The procedure (1) successfully removed lipids and proteins derived from insects with the satisfied recoveries of the target analytes at two spiking levels of 5 ㎍/kg and 10 ㎍/kg. The recoveries were 87.6-112.9 ± RSD 4.2-4.6% for fenoxanil, 83.3-87.6 ± RSD 1.3-4.6% for thiobencarb, and 76.9~82.0 ± RSD 3.4-7.2% for fludioxonil. Method detection limit was 1.0 Mg/L. The optimized method was also applied to real samples from 30 different farms across the nation. These data can be used to set maximum residual limit for the residues in edible insects in response to positive list system. The method developed in this study is expected to be also applied to other edible insects or matrices having high fat and protein contents.
Control of Bemisia tabaci in Greenhouse Tomato using Systemic Insecticide-treated Trap Plant
Leesun Kim,Meeja Seo,Dagyeong Jeong,Bo Yoon Seo,Jonghwa Lee,In-hong Jeong 한국농약과학회 2023 농약과학회지 Vol.27 No.4
This study aimed to develop a new control method of Bemisia tabaci using a tobacco plant treated with systemic insecticide in tomato greenhouse cultivation. In the preference tests, it was demonstrated that tobacco is a better B. tabaci attractant than the seven tested solanaceous plants. Most importantly, B. tabaci prefers tobacco (65%) to the main host plant, tomato (35%). Among 6 systemic insecticides, dinotefuran WG was highly effective (mortality > 97.3%) against B. tabaci and exhibited less or non-disruptive tendency on insect behavior on dinotefuran-treated tobacco plants. The dinotefuran concentrations in tobacco leaves obtained using modified QuEChERS method with liquid tandem mass spectrometry rapidly increased over time, and mortality of B. tabaci populations recovered from tobacco leaves was over 90% within 24 hrs after insecticide treatment. The mortalities were consistently over 90 to 100% until 35 d. The upper leaves of tobacco showed slightly higher concentration of insecticide, including the resultant B. tabaci mortalities than the lower leaves. Compared with that of greenhouse with no tobacco plants (100%), the population of B. tabaci in greenhouses with two and three insecticide-treated tobacco plants increased by 53%-63% and 22% respectively. These results confirmed that a dinotefuran-treated tobacco plant can be an effective trap plant for the control of B. tabaci in tomato cultivation greenhouses.
Leesun Kim,Yeyeon Kwak,Mi-Ja Seo,Dagyeong Jeong,In-hong Jeong 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.10
This study aimed to develop a new eco-friendly control method of B. tabaci using a trap plant treated with systemic insecticide. Due to high preference, tobacco plant was selected as a trap plant among solanaceous plants. Out of all 6 systemic insecticides, dinotefuran WG showed the highest mortality (> 97.3 %) against B. tabaci. The concentrations of dinotefuran in tobacco leaves after systemic treatment showed the sharp increase in the pesticide from 2 hrs until 48 hrs (2 d) and steady increase over 35 d. The mortality of B. tabaci in tobacco leaves was about 80% at 9 hrs after treatment and over 90% at 1, 2, 3, 6, 9, and 35 d after treatment. The number of B. tabaci was counted every week after dinotefuran-treated tobacco pots were placed in the tomato greenhouse cultivation. The density of the insects with 3 tobacco plants was app. 75% lower than the control (w/o tobacco plant).