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Lee-Stadelmann, OkYoung,So, In-Sup,Li, Yong-Fang,Zhao, Yu-Fen 제주대학교 방사능이용연구소 1995 연구보고 Vol.9 No.-
Interaction of N-(diisopropyloxyphosphoryl)-amino- acids (DIPP-AA) with membrane lipids was analyzed by evaluating their effects on membrane fluidity. Water and methyl urea permeability were used as probes for membrane fluidity. Permeability of epidermal cells of Pisum sativum stem base and the inner epidermis of the Allium cepa bulb scale was measured by standard osmotic method after their exposure to a 5mM solution of each DIPP-AA for 20 min. Treatments with the phosphoryl derivates of three polar and one charged amino acids (serine, threonine, asparagine, and histidine) significantly increased water permeability about two fold compared to the untreated controls. Pretreatment with the other amino acids and their derivatives resulted in only small(increase or decrease) or no significant changes in water permeability. The Pf value of Pisum sativum epidermal cells(untreated controls) was 1.3±0.40x10?? cm sec-1. Free amino acids and their DIPP-derivates equally increased urea and methyl urea permeability but much less than water permeability. Methyl urea permeability was 10.26±0.93 x 10-⁴ cm/sec. These results suggest that DIPP-AAs interact with the phospholipid bilayer of the plasmamembrane: maybe the diisopropyl tail of the DIPP-AA anchors the molecule in the acyl part of the membrane while the AA moiety in the acyl part of the membrane while the AA moiety interacts with the polar region of the membrane. These structural fitness of amino acids may lead to AA specific changes in membrane fluidity and packing density.
Lee-Stadelmann, Ok Young,Lee, Seungwoo,Chung, Haejoon,Guo, Quansheng,Kim, Myungwon,Pak, Chunho,Hackett, Wesley P. 경희대학교 유전공학연구소 1991 遺傳工學論文集 Vol.3 No.-
In vitro adventitious bud formation was investigated in 3 hybrid Populus clones with varying degrees of regeneration potential. Longitudinal and transverse wounding treatment of 1.0 cm leaf midvein and petiole explants both increased the shoot regneration capacity, more effectively with transverse wounding. Use of micro-cross sections (400 um in thickness) of leaf midvein or stem internode greatly improved regeneration capacity in all 3 clones. Supplementary calcium nitrate (7.05 to 9.4 mM) with optimal BA and NAA concentrations enhanced regeneration potential of micro- cross sections of 2 clones, but not of the most recalcitrant clone. Micro-cross section are excellent explant for obtaining large numbers of uniform adventitious shoots from minimum amount of material in a short regeneration time.
Lee, Dong Un,Li, Jingde,Park, Moon Gyu,Seo, Min Ho,Ahn, Wook,Stadelmann, Ian,Ricardez-Sandoval, Luis,Chen, Zhongwei Wiley (John WileySons) 2017 ChemSusChem Vol.10 No.10
<P>The present work introduces spinel oxide nanocrystals self-assembled into mesoporous spheres that are bifunctionally active towards catalyzing both the oxygen reduction reaction (ORR) and the oxygen evolution reaction (OER). The electrochemical evaluation reveals that (Ni, Co)(3)O-4 demonstrates a significantly positive-shifted ORR onset and half-wave potentials [-0.127 and -0.292 V vs. saturated calomel electrode (SCE), respectively], whereas Co3O4 results in a negative-shifted OER potential (0.65 V vs. SCE) measured at 10 mA cm(-2). Based on the DFT analysis, the potential at which all oxygen intermediate reactions proceed spontaneously is the highest for (Ni, Co)(3)O-4 (U= 0.66 eV) during ORR, whereas it is the lowest for Co3O4 (U= 2.09 eV) during OER. The high ORR activity of (Ni, Co)(3)O-4 is attributed to the enhanced electrical conductivity of the spinel lattice, and the high OER activity of Co3O4 is attributed to relatively weak adsorption energy promoting rapid release of evolved oxygen.</P>
Optimization of Cymbidium Transformation System by the Particle Gun Techniques
Hong, Kyung-Ae,Lee-Stadelmann, Ok Young,U, Zang-Kual,So, In-Sup,Cheong, Choong-Duk 제주대학교 방사능이용연구소 1995 연구보고 Vol.9 No.-
Process of particle bombardment for efficient transformation of Cymbidium virescence rhizome microcross sections was investigated using Biolistic particle delivery system with pBI121 harboring the β-glucuronidase(GUS) and the neomycin phosphotransferaseII(NPTII), and pBI221 containing GUS. The best result was obtained from the combination of 1.11㎛ tungsten particles coated with pBI121, 77.33 ㎏/㎠ helium pressure, 6.35 ㎜ gap distance, and 3.8 ㎝ target distance. Transient expression of the reporter gene, GUS, bombarded into the rhizome microsections was observed by the histochemical assay. The selectable marker gene, NPT II, delivered by bombarding the tungsten particles coated with the plasmid DNA was identified using the polymerase chain reaction technique.
Re-evaluation of FDA as a vital Staining for Plant Cells
Chung,Insun,O.Y.Lee-Stadelmann,So,Insup 濟州大學校 亞熱帶農業硏究所 1989 亞熱帶農業硏究 Vol.6 No.-
살아있는 세포의 활성을 검정하는데 널리 이용되고 있는 FDA와 Uranin에 대한 Urea의 투과성과 세포질 유동 그리고 세포 대사 억제물질 처리 후의 발광 정도를 조사하여 공시한 2가지 약제(FDA, Uranin)의 이용에 대한 정확한 평가를 하기 위하여 양파의 표피 조직과 완두콩의 2차 표피 조직을 대상으로 본 실험을 실시하였으며, 얻어진 결과는 다음과 같다. 1. 양파의 표피 조직을 이용한 표준 원형질 유동 속도는 7㎛/sec.이며 2시간이 경과한 후에도 속도의 변화는 없었지만, 식물 세포 대사 억제로서의 DNP와 NaN 처리에서는 시간이 경과함에 따른 모든 농도에서의 유동 속도가 감소하였다. 특히 DNP 2시간 처리후 120분이 경과되면 모든 원형질 유동이 정지됨을 볼 수 있었다. 2. FDA나 Uranin 염색에 의한 형광의 밝기 정도는 대사 억제물질이 처리된 후에도 현미경 하에서 육안으로 뚜렷이 구별할 수 없었다. 그러나 한편 형광량 측정 현미경으로 측정된 수치는 FDA가 Uranin과 비교할 때 7배나 더 밝은 것으로 나타났는데 염색약제로 희석되는 배율은 Uranin이 FDA보다 10배 높은 농도의 용액이다. 3. FDA와 Uranin의 전처리에서는 원형질 분리 현상이 기대했던 바와 같이 어떠한 영향도 주지 않았으나, Urea 투과율을 비교해보면 FDA가 100%일 때 Urnin이 35%의 비율로 투과됨을 알 수 있었다. 결론적으로 FDA는 죽은 세포를 검정하는데 적합한 약제일수는 있지만 대사적으로 활성있는 세포의 활성을 조사하는데는 부적합함을 알 수 있다. 따라서 생체 염색 방법은 식물세포의 체내 대사의 활성을 검정하는데 썩 좋은 방법이라고 할 수 없기 때문에 특히 분리된 원형질체의 활성을 검정하는데는 앞으로 누구나가 신뢰할 수 있는 새로운 기술이 요망되는 바이다. The main purpose of this investigation was to evaluate the accurate use of FDA(Fluorescein diacetate) and Uranin(soluble fluorescein). Which were well known vital staining agent to testing the vitelity of living cell, compared by the response of urea permeability. cytoplasmic streaming and fluorecing quality after treatment of two kind of metabolic inhibitor in the protoplasm of adaxial epidermal cells of the Allium cepa bulb scale and sub-epidermal cells of the Pisum sativum. The results obtained are as follows : 1. The speed of cytoplasmic streaming measured with onion epidermal cells in control was about 7 ㎛/sec. and did not change for 2 hours, whereas, for all concentration used, both metabolic inhibitors decreased streaming speed as time elapsed. Especially, cells treated with DNP for 120 min, almost stopped cytoplasmic streaming in 2 hours. 2. Brightness of fluorescence by FDA or Uranin staining could not be distingished by naked eyes under the microscopic observation even after inhibitor treatment. From the data measured by micro-fluometer(Zeiss epifluorescent microsoope), on the other hand, the cells stained with FDA exhibited brighter fluorescence than with uranin by 7 times although concentration of uranin in staining solution was 10 times higher than FDA. 3. As expected, plasmolysis itself was not affected with the pretreatment of FDA and Uranin : however the permeability constant increased by almost 100% and by 35% with pretreatment of FDA and Uranin respectively. In conclusion, FDA seems not to be good to identify metabolically active cells despite its good performance for teating dead cells. However, vital stainings are not highly recommended to screening metabolically active cells. Therefore new method should be developed for evaluating metabolic status of isolated protoplasts.
Genetic Enhancement of Cold Tolerance of Cymbidium by Introducing ω-3 Fatty Acid Desaturase Gene
So,In-Sup,Hong,Kyung-Ae,U,Zang-Kual,Lee-stadelmann,Ok-Young,Song,Sung-Jun 濟州大學校 放射能利用硏究所 1996 연구보고 Vol.10 No.-
Protocorm like bodies(PLBs) were micro-sectioned by a vibratome, and the conditions for in vitro culture were optimized. PLBs were induced from the meristem of Cymbidium side-bud and proliferated on Kyoto medium (4 ml/liter of Hyponex) supplemented with 1 mg/liter of α-naphthaleneacetic acid(NAA). The good formation of adventitious PLBs was made from 400㎛ thick micro-sectioned PLBs. The growth and proliferation of PLBs reformed was most effective in Kyoto medium containing 2 or 4 ml/liter of Hyponex and 1.0 mg/liter of NAA and the shoot formation was promoted by the addition of 1 mg/liter of benzyl adenine(BA). As transient expression of β-glucuronidase(GUS) gene could be identified in the bombarded PLB cells, the micro-sectioned PLBs were bombarded with the microprojectiles coated with the plasmid pBIVA1 containing NPT II and fad7. Now the cold tolerant transformants of reformed PLBs are being selected on the kanamycin medium.