Identification of Nickel-resistant Bacteria from Metal Contaminated Soil and Potential Mechanism by Genome Sequencing

Seul Lee(이슬),Anamika Khanal,Junkoo Yeo(여준구),Kathyleen Nogrado,Ahyeon Cho(조아현),Yoonjin Song(송윤진),Miji Kwon(권미지),Ji-Hoon Lee(이지훈) 한국토양비료학회 2018 한국토양비료학회 학술발표회 초록집 Vol.2018 No.5

Identification of determinants of the utilisation of acupuncture treatment using Andersen's behavioural model

Lee, Ye-Seul,Lee, In-Seon,Kim, Song-Yi,Lee, Hyangsook,Park, Hi-Joon,Lee, Hyejung,Kim, Sung-Hoon,Chae, Younbyoung THE BRITISH MEDICAL ACUPUNCTURE SOCIETY 2015 ACUPUNCTURE IN MEDICINE Vol.33 No.2

<P><B>Objectives</B></P><P>The utilisation of healthcare services is the result of a complex decision-making process with different determinants. This study aims to identify the determinants of the utilisation of acupuncture treatment using Andersen's behavioural model and quality of life.</P><P><B>Methods</B></P><P>In this study we reviewed Korean Health Panel data, a nationally representative set of data, and analysed the utilisation of outpatient acupuncture services during 2011. A two-part model, which included predisposing, enabling and need factors of Andersen's behavioural model in model 1 and the additional factor of health-related quality of life (HRQOL, EQ-5D Korean index) in model 2, was analysed by logistic analysis to identify determinants of acupuncture service usage among subjects who visited traditional Korean medical clinics.</P><P><B>Results</B></P><P>The higher frequency of acupuncture service usage among patients who visited traditional Korean medical clinics was associated with gender, age, number of chronic diseases, physical disabilities and HRQOL of the subjects.</P><P><B>Conclusions</B></P><P>This study shows that the important determinants of the frequency of acupuncture treatment utilisation are gender, age, number of chronic diseases and the presence of physical disabilities. Our findings contribute to understanding the characteristics of patients who use acupuncture treatment and may be used as a basic resource for related policy making by government officials and medical professionals.</P>

Plant Analysis Methods for Evaluating Mineral Nutrient

Lee, Ye-Jin,Sung, Jwa-Kyung,Lee, Seul-Bi,Lim, Jung-Eun,Song, Yo-Sung,Lee, Deog-Bae,Hong, Suk-Young 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.2

Analysis of mineral nutrients in plant is required for evaluating diagnosis of plant nutritional status. Pretreatment procedure for the analysis of plant can be varied depending on elements to be analyzed. Wet-digestion is suitable for total nitrogen, phosphate and cations, however, digestion solution including nitric acid is not suitable for nitrogen analysis. Incineration procedure is required to analyze chloride, silicate and total sulfur. After digestion, total nitrogen is analyzed by Kjeldahl method, and phosphate is detected at 470nm by colorimetric analysis with ammonium meta vanadate. Cations and micro elements are determined by titration or colorimetry, also, these elements can be measured by Atomic absorption spectrometer (AAS) or Inductively coupled plasma spectrometer (ICP).

Analysis of Soil Total Nitrogen and Inorganic Nitrogen Content for Evaluating Nitrogen Dynamics

Seul-Bi Lee,Jwa-Kyung Sung,Ye-Jin Lee,Jung-Eun Lim,Yo-Sung Song,Deog-Bae Lee,Suk-Young Hong 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.2

Various methods for assessing soil total nitrogen (TN) and inorganic N content have been developed to manage nutrient and to understand N cycle in soil. This paper address the technical procedures in arable soil samples to conduct soil sampling, sample preparation, and measuring total N and inorganic N. Among various methods for measuring soil total nitrogen contents, Kjeldahl distillation and Indophenol blue method have widely used due to reliability and economic advances. Also, two methods can analyze more samples at the same time compared with other nitrogen measuring methods. For evaluating inorganic N content, mainly in forms of nitrate-N (NO₃<SUP>-</SUP>-N) and ammonium-N (NH₄<SUP>+</SUP>-N), extraction with a single reagent such as 2M KCl has been employed, followed by Kjeldahl distillation or indophenol blue methods.

The Effect of Livestock and Food Waste Compost on Rice Yield and Nutrient Utilization Efficiency according to Different Nitrogen Fertilizer Treatments Rates

Song Rae Cho,Ye-Jin Lee,Chan-Wook Lee,Byeong-Geun Hyun,Yo-Sung Song,Chul-Hyun Ryu,Yang Min Kim,Seul-Bi Lee 한국토양비료학회 2021 한국토양비료학회지 Vol.54 No.4

The amount of livestock manure generated was 1,532 hundred tons/day as of 2019, and it increased by about 11% over 10 years. It has been raised as a cause of soil and water pollution due to the continuous overspray of livestock compost on farmland. It is essential to calculate an appropriate amount of livestock compost that can replace chemical fertilizers in order to manage local environment-friendly nutrients without problems caused by the use of livestock compost. In addition, food waste is mostly agricultural products-derived materials, and it is necessary to establish a comprehensive management system for agricultural cycle use. In this study, to confirm the compost mixed with livestock and food waste as an alternative to chemical fertilizer, comparison of the basic replacement of chemical fertilizer and livestock, the replacement of the mixed compost including food waste, the replacement of the total nitrogen amount of livestock compost, and the replacement of the total nitrogen amount of the mixed compost. Experiments were conducted to confirm the effect of each compost on crop production. Commercially available livestock compost (_L) and mixed compost (food waste and live-stock)(_M) were used as the material for the experiment. Treatments were control, NPK, 5N_L, 5N_M, 7.5N_L, 7.5N_M, 9N_L, 9N_M. The comparison between livestock compost and mixed compost, mixed compost has 30% high crop productivity. The nitrogen use efficiency was shown as 7.5N_M > 5N_M > 5N_L > NPK > 7.5N_L > 9N_M > 9N_L. For high yield and nutrient uptake, fertilizer partial replacement (55.6% or 83.3% than total replacement) will be more efficient than 100% total nitrogen requirement replacement.

Effect of Optimum Nutrient Management on Productivity and Nitrogen Balance in Rice Cultivation: A Review

Seul-Bi Lee,Yang-Min Kim,Ye-Jin Lee,Yo-Sung Song,Cheol-Hyun Ryu,Deog-Bae Lee,Chan-Wook Lee,Chang-Hoon Lee,Jwakyung Sung 한국토양비료학회 2019 한국토양비료학회지 Vol.52 No.4

Given continuous needs for a public duty of agriculture and rural area, an effort to improve nutrient management in agricultural sector is strongly required. As a measure to solve these problems, Korean government (MAFRA) introduced the promotion policy called as “Agro-Environment Conservation Program (AECP)” in 2018 and has expanded 5 projet sites in 2019 from 3 in 2018. The program has a variety of agricultural activity to conserve soil, water, air, ecology and landscape. Of those, the nutrient input activity which is one of sub-activities is closely involved in not only crop productivity but also water quality and air pollution. Therefore, in order to lead a soft landing of AECP, it is essential to evaluate the conservation effects in a scientific point of view. In this respect, we focused on evaluating the effect of recommended nutrient input activities, standard fertilization and soil test fertilization, on rice productivity and nitrogen balance. The effect of nutrient management which is explained by rice yield and nitrogen balance was estimated with the combination of soil characteristics and rice varieties. Rice yield by recommended fertilization was 654 kg per 10a, 6% lower than control, but there was no statistical significance. By contrast, nitrogen balance was 4.7 kg lower, which means great improvement, in the recommended than the control. Conclusively, the recommended fertilization like soil test fertilization is expected to be useful as a measure to reduce nutrient balance, to ensure crop productivity, and finally to perform a public role of agriculture.

Fertilizer Use Efficiency of Taro (Colocasia esculenta Schott) and Nutrient Composition of Taro Tuber by NPK Fertilization

Lee, Ye-Jin,Sung, Jwa-Kyung,Lee, Seul-Bi,Lim, Jung-Eun,Song, Yo-Sung,Lee, Deog-Bae 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.4

The objectives of fertilizer recommendation are to prevent the application of excessive fertilization and to produce target yields. Also, optimal fertilization is important because crop quality can be influenced by fertilization. In this study, yields and fertilizer use efficiency of Taro (Colocasia esculenta Schott) were evaluated in different level of NPK fertilization. N, P and K fertilizer application rates were 5 levels (0, 50, 100, 150, 200%) by practical fertilization ($N-P_2O_5-K_2O=180-100-150kg\;ha^{-1}$), respectively. In the N treatment, the yields of Taro tuber were about $33Mg\;ha^{-1}$ from 90 to $360kg\;ha^{-1}$ N fertilization. However, the ratio of tuber to total biomass decreased with increasing N fertilization rate. In the P and K treatments, yields of Taro tuber were the highest at $150kg\;ha^{-1}$ fertilization. Fertilizer use efficiency was decreased by increase of N and K fertilization. Crude protein of Taro tuber was the highest at practical fertilization. Sucrose content of tuber was influenced by phosphate application.

Yield and Free Sugar Contents of Burdock (Arctium lappa L.) depending on Nitrogen Levels

Lee, Ye-Jin,Sung, Jwa-Kyung,Lee, Seul-Bi,Lim, Jung-Eun,Song, Yo-Sung,Lee, Deog-Bae 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.6

Excessive nitrogen fertilization influences crop yields and quality as well as environmental pollution. In this study, yields, nitrogen use efficiency and free sugar contents of burdock (Arctium lappa L.) were evaluated at different levels of nitrogen fertilization. Nitrogen fertilizer was applied at 5 levels (0, 50, 100, 150, 200%) based on the conventional fertilization ($N=230kg\;ha^{-1}$), and phosphate and potassium fertilizer were treated by conventional P and K fertilization ($P_2O_5-K_2O=140-210kg\;ha^{-1}$) in all plots. The root yields of burdock were the highest in N 100~150% treatment plots. Nitrogen use efficiency and nitrogen recovery decreased from over N 150% treatment. Nitrogen uptake of root was greater than that of shoot in N 50~200% treatments. Fructose contents in root were inversely proportional to the level of nitrogen fertilization. As considering nitrogen recovery and root quality, economical burdock yield was obtained in N $230kg\;ha^{-1}$.

Analysis of Soil Total Nitrogen and Inorganic Nitrogen Content for Evaluating Nitrogen Dynamics

Lee, Seul-Bi,Sung, Jwa-Kyung,Lee, Ye-Jin,Lim, Jung-Eun,Song, Yo-Sung,Lee, Deog-Bae,Hong, Suk-Young 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.2

Various methods for assessing soil total nitrogen (TN) and inorganic N content have been developed to manage nutrient and to understand N cycle in soil. This paper address the technical procedures in arable soil samples to conduct soil sampling, sample preparation, and measuring total N and inorganic N. Among various methods for measuring soil total nitrogen contents, Kjeldahl distillation and Indophenol blue method have widely used due to reliability and economic advances. Also, two methods can analyze more samples at the same time compared with other nitrogen measuring methods. For evaluating inorganic N content, mainly in forms of nitrate-N ($NO_3{^-}-N$) and ammonium-N ($NH_4{^+}-N$), extraction with a single reagent such as 2M KCl has been employed, followed by Kjeldahl distillation or indophenol blue methods.

Growth Stage-Based Fertigation Guideline for Greenhouse Spring Chinese Cabbage

Seul-Bi Lee,Yang-Min Kim,Ye-Jin Lee,Yo-Sung Song,Deog-Bae Lee,Jwakyung Sung 한국토양비료학회 2019 한국토양비료학회지 Vol.52 No.4

With increasing fertigation, the precise fertigation schedule by crop growth stage has been consistently required as a guideline for stable crop production. The aim of this study is to develop the fertigation guideline for spring cabbage. To achieve this goal, we firstly investigated the nutrient uptake pattern of Chinese cabbage, which grown in pots filled with sandy loam soil in a greenhouse from April to June in 2018, and determined optimal nutrient (N, P and K) supply by growth stages. Based on soil NO₃-N based recommendation, nitrogen (N) supply was divided into 4 levels (0.5N, 0.75N, 1.0N and 1.5N). Soil NO₃-N concentrations somewhat differed from growth stages, and 1.5N represented the highest level. Chinese cabbage showed linear growth patterns and there was no clearly significant difference in growth and nutrient uptake by N supply levels except for 0.5N treatment. The relative growth (expressed as a dry weight) of Chinese cabbage occupied 60% and 40% of total accumulated growth (average of 0.75 and 1.0N) at 40 and 60 days, respectively. Mineral uptake (N, P, K) of Chinese cabbage by growth stages showed a linear pattern in general. N was taken up 54% and 46% of total uptake, P uptake 56% and 44%, and K uptake 59% and 41% at 40 and 60 days, respectively. Conclusively, we carefully suggest that the fertigation guideline for Chinese cabbage could save fertilizer supply through daily nutrient demand and target yield, and thus alleviate the problems in relation to nutrient accumulation in greenhouse soils.