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Streptococcus mutans에서의 세포외다당류 합성효소의 활성에 미치는 수종약물의 영향
오귀옥,정동균 대한구강생물학회 1980 International Journal of Oral Biology Vol.4 No.1
Streptococcus mutans synthesize insoluble glucans from sucrose by the cell-associated and extracellular glucosyltransferase and these glucans gives the characteristic adhesive nature to the plaque which contains S. mutans. As the insoluble glucans in dental plaque apparently play a cardinal role in the cariogenic otential of S. mutans inhibition of glucan synthesis could be a contributory factor in the prevention of dental plaque, and ultinatively dental caries. The water extract of capsicum and several antibacterial compounds, including surface-active agents previously shown to be plaque inhibiting, were tested for their effect on glucan synthesis by an extracellular glucosyltransferase preparation from S. mutans B-13. The results were as follows. 1. Crude enzyme preparation was found to contain 0.23 unit/ml of enzyme activity (1 unit transforms 1 μmole of sucrose in 1 minute) as measured turbidimetrically and also by measuring the incorporation into polysaccharide of radioactivity from radioactive sucrose. 2. Surface-active agents (chlorhexidine, cetylpyridinium, sodium lauryl sulfate) were found to be potent inhibitors of enzyme activity. Both electrostatic and hydrophobic interactions appear to be occurring between the cation of test agents and the glucosyltransferases, with hydrophobic interaction being more important for enzyme inhibition. 3. Iodine preparation (providone iodine) was also found to inhibit enzyme activity perhaps through the iodination mechanism. 4. Some unknown agents in water extract of capsicum could stimulate the enzyme activity in the presence of strong inhibitory effect of glucose.
Arachidonic acid 대사산물이 배양골조직의 골흡수에 미치는 영향에 관한 연구
오귀옥,김세원 대한구강생물학회 1989 International Journal of Oral Biology Vol.13 No.2
This experiment was performed to study the arachidonic acid metabolites (prostaglandin E_2, leukotriene B_4 and HETE) and indomethacin of the bone resorption and the effect of indomethacin on the PGE_2-stimulated bone resorption in the organ culture. Ulnae and radii or calvaria were removed from 19-day fetal rats prelabelled by subcutaneous injection of 200 μCi ^45CaCl_2 into mother rats at the 17th day of gestation. The control groups were cultured in fresh BGJb media, while experimental groups were cultured in media containing PGE_2 (10^-6M), indomethacin (10^-5M), leukotriene B_4 (0.1pM, 1pM, 10pM, 100pM and 1nM) or HETE (0.1pM, 1pM, 10pM, 100pM and 1nM) for 48 or 72 hours. Radioactivities of ^45Ca released into media and remained in bone after culture were respectively determined. The effects of each drugs were observed by the ratios of %-release of ^45Ca between control and experimental group. The observed results were as follows. 1. PGE_2, at concentration of 10^-6M, increased ^45Ca release significantly after 24 and 48 hours of culture. 2. Indomethacin, at concentration of 10^-5M, decreased ^45Ca release after 24 and 48 hours of culture, but there was no significant difference. 3. LT and HETE, at all concentrations, had no effect on the bone resorption after 72 hours of culture. 4. Indomethacin, at concentration of 10^-5M, had no effect on the PGE_2-stimulated bone resorption after 72 hours of culture.
치주질환에 있어서의 조직파괴인자 및 억제약물에 대한 연구
김오환,김형섭,오귀옥 대한구강생물학회 1988 International Journal of Oral Biology Vol.12 No.2
Arachidonic acid(AA) metabolites are known to play an important role in the development of inflammation and pathogenesis of periodontal disease. The bone resorbing activity of PGE_2 and elevated level of prostaglandins and thromboxane in inflamed gingiva, which are cyclooxygenase(C) products, has been well documented. However, since recent studies provide evidence that gingival tissue metabolizes AA mainly through lipoxygenase(L) pathway, we have started this study to show the exact profile of AA metabolism in gingival tissue. Fourteen individual periodontal pocket tissue(PPT) samples were obtained from patients with advanced periodontitis and incubated separately with ^14C-AA(0.2μCi). The tissue lipid extracts were separated by means of thin layer chromatography(TLC), and analyzed by means of autoradiography and TLC analyzer. Our work show that ^14C-AA metabolism in PPT is mainly via the L pathway(L products/C products=6.17) and that 12-HETE & 15-HETE(55.73% of total formed metabolites), to a lesser extent 5-HETE(6.21%), are the major L products formed. C products thromboxane B_2 (4.32%), 6-keto-PGF_1α & PGE_2 (4.42%) were also detected in much smaller amounts than L products. Indomethacin well-known C inhibitor, also inhibited L as well as C, that is the result similar to the reports of Paajanen et al.(1982) and Lessard et al.(1986). This phenomenon is being further investigated by use of partially purified L system.
김형섭,오귀옥,장기완 대한구강생물학회 1991 International Journal of Oral Biology Vol.15 No.2
This experiment was performed to study the effect of the arachidonic acid metabolites(PGE_2, LTB_4, HETEs) on the bone resorption in the organ culture. The observed results were as follows: 1. PGE_2, at concentration of 10^-6M, increased ^45Ca release significantly after 72 hours of culture. 2. LTB_4 or HETEs, at all concentrations, had no effect on the bone resorption after 72 hours of culture. 3. LTB_4 or HETEs did not change the bone resorbing activity of PGE_2 bone resorption after 72 hours of culture.
丁東均,李種昕,李孝宰,金各均,吳貴玉,鄭泰英 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1
The urinary fluoride concentration and the degree of dental fluorosis of all the schoolboys attending Chimoon primary school in Baikku-myun, Kimjae-kun and the fluoride concentration of drinking water of those areas were investigated in an effort to elucidate the relation between them. The urinary fluoride concentration and the fluoride concentration of driking water were measured by fluoride ion electrode, and dental fluorosis was surveyed and analysed by Dean and McKay's and Horowitz's method. 1) The mean urinary fluoride concentration and the mean fluorosis index of all the schoolboys were 1.89ppm (0.12-12.2ppm) and 0.79 (0-1.99). The mean fluoride concentration of drinking water of those areas was 0.61ppm (0.027-2.66ppm). 2) The mean urinary fluoride concentration and the mean fluorosis index of the schoolboys residing in Yookang-ri 5-ku, Baikku-myun (Dongja-po) were the highest (3.23ppm, 1.99) among the statistics for the schoolboys residing in different areas. Incidentally the fluoride concentration of drinking water for that area was also the highest (1.99ppm). 3) The urinary fluoride concentration was two to ten times as high as the fluoride concentration of drinking water regardless of the resident area of schoolboys. 4) There was no significant seasonal change in fluoride concentration of drinking water collected from the well in the resident area of schoolboys. 5) There showed a relatively parallel relationship between the urinary fluoride concentration and the fluorosis index of schoolboys. 6) The urinary fluoride concentration and the fluorosis index of schoolboys tend to increase with age increasing.
정동균,이종흔,김각균,이공훈,오귀옥,정태영 대한구강생물학회 1980 International Journal of Oral Biology Vol.4 No.1
Those studies were performed to elucidate the effects of several dietary spices on dental caries and to determine which component of spices induced the change of incidence of dental caries in Sprague-Dawley rats which were supplied from SNU animal house. For this study, 132 rats (rats weighing 90-140 gm) were chosen at random with the same number of both sexes and divided into eleven experimental groups which contained the differential components of drinking water and diets for control and experimental group. Control group was fed standard cariogenic diet and tap water. In experimental groups, rats were fed standard cariogenic diet supplemented with capsicum (10%), ash capsicum (1, 10%), garlic (5%), common salt (3%), and analytical salt (3%) for 80 days. Drinking water was tap water containing water extract of capsicum (1, 10%), water extract of garlic (1.1%), and fluoride (50ppm). The results were as follows. 1. 54.6 in caries score was recorded in control group. 2. Rats which drank water containing 50ppm of fluoride showed 18.4% decrease in caries score. 3. Capsicum-fed rats showed 10.7% (capsicum 10%) increase in caries score and rats which drank water containing water extract of capsicum (1, 10%) showed 14.1% (w.e. capsicum 1%) and 0.16% (w.e. capsicum 10%) increase in caries score. But ash capsicum-fed rats showed 18.7% (ash capsicum 1%) and 7.3% (ash capsicum 10%) decrease in caries score. 4. Garlic-fed rats showed 22.1% (garlic 5%) decrease in caries score. But rats which drank water containing 1.1% of water extract of garlic showed 24.1% increase in caries score. 5. 58.4% and 68.0% increase in caries score were recorded in rats which were fed diet containing common salt (3%) and analytical NaCl (3%), respectively.
정태영,정동균,이효재,김각균,오귀옥,이종흔 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1
Insoluble extracellular polysaccharides synthesized by Streptococcus mutans type d strain B-13 were analysed in an effort to elucidate their chemical composition. Dextransucrase produced in supernatant was also studied for its activity. 1. The microorganism produced 2.10g of total insoluble fraction which contained 1.59g of insoluble polysaccharide and 0.41g of bacterial mass per 800ml of culture medium. 2. Insoluble fraction contained 26.1㎍/mg of nitrogen and 746.4㎍/mg of insoluble extracellular polysaccharides. Ketohexose content of polysaccharides was 7.12%. 3. Streptococcus mutans strain B-13 was capable of producing 4.10g insoluble extracellular polysaccharide per 1.0g of bacterial mass. 4. Dextransucrase liberated 5.11mg of hexose per 1ml of culture supernatant.
鄭東均,鄭素英,李孝宰,金各均,吳貴玉,李種昕 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1
Two-part experiment was conducted to elucidate the effects of several trace elements, rice and spices on caries rate in Korean albino rats. Rats were selected into two large groups according to their weight ; one group (for part I experiment) consists of rats weighing 140-200gm and the other group (for part II experiment) consists of rats weighing 76-109gm, and the same experiment was conducted independently for both groups. Rats were fed standard cariogenic diet supplemented with rice (21, 53%), capsicum (1, 10%), garlic (0.5, 5 or 10%), phosphorus (2%), sodium chloride (3%, reagent grade), or table salt. Drinking water was tap water containing cold water extract of capsicum (0.1%) alcoholic extract of capsicum (0.1%), water extract of ginseng (2%), fluoride (10, 50 or 100ppm) or 50ppm of Mg, Zn, Mo, Sr, Mn of Fe. The results were as follows. 1. 24.9%, 51.0% and 54.3% decrease in caries score were recorded for rats which drank water containing 10, 50 and 100ppm of fluoride in part I experiment. In part II experiment caries score decreases 51.8% and 56.9% for 10ppm and 50ppm fluoride content. 2. Capsicum-fed rats showed 51.7% (capsicum 1%) and 58.1% (capsicum 10%) decrease in caries score in part I experiment. 21.5% and 33.6% decrcase were recorded for part II experiment. 3. Garlic-fed rats showed 44.5% (garlic 0.5%), 23.0% (garlic 5%) and 53.7% (garlic 10%) decrease in caries score. 4. Sodium chloride-fed rats showed 122.6% (NaCl 3%) increase in caries score. 5. Rats which were fed diet containing 2% Na_2HPO_4 showed 26.7% decrease in caries score and further 41.6% decrease when 10ppm fluoride was given simultaneously. 6. 28.4% and 31.5% decrease in caries score were recorded for rats which drank water containing 50ppm of Mg and Zn.
이종흔,정동균,김중수,이효재,김각균,오귀옥,정태영 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1
Mineral analyses of water samples from several areas of the Mankyung River and the Dongjin River regions in Chonrabukdo where dental fluorosis was observed, which had been conducted between June in 1978 and January in 1979, have shown the following results. In general, the mean calcium concentrations were high in the areas where the mean concentrations of phosphorus and fluoride were low. And in the same area where dental fluorosis was particularly observed, seasonally a few minerals were variable─namely, calcium, phosphorus, and fluoride. Those samples of high fluoride concentration have higher phosphorus concentration, but calcium concentrations were the opposite phenomenon. Particularly, fluoride concentrations were remarkably greater during the cold season(January) than during the rainy season(July).
정동균,정태영,이종흔,김중수,오귀옥,이효재 대한구강생물학회 1980 International Journal of Oral Biology Vol.4 No.1
This experiment was conducted to elucidate the relation between the urinary fluoride concentration and the fluorosis index of residents in Mankyung River valley, especially in 3 areas (Yookang-ri, Koje·Yoochun, Shinbok·Yongkang) with high fluoride concentration in drinking water. The urinary fluoride concentration was measured by fluoride ion electrode, and dental fluorosis was surveyed and analysed by Dean and McKay's and Horowitz's method. 1. There was no reletionship between the urinary fluoride concentration and age. 2. 11-20 years age groups has revealed the highest fluofosis index. In this group : 3. There showed a parallel relationship between the fluorosis index and the urinary fluoride concentration. 4. DMF rates of residents in Yookang-ri, Koje and Shinbok are 59.2, 48.4 and 47.1%, respectively. 5. There showed a parallel relationship between the years of residence within 8 years after birth and the fluorosis index.