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SangMi Han,KwangGill Lee,JooHong Yeo,SoonOk Woo,HaeYong Kweon,YouYoung Jo,Peter Molan 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05
Since the ancient times the therapeutic application of honeybee venom (BV) is practised and persisted until the present days. Resistant bacteria are in emergence and some drugs no longer have an antimicrobial action. To purify the melittin known as antibacterial peptide, five major peptidergic subfractions were separated, purified and identified from the whole BV. We investigated the antibacterial activity of whole BV and purified melittin against Staphylococcus aureus by the minimum inhibitory concentrations (MIC) and the postantibiotic effect (PAE). The MIC of whole BV for S. aureus was 0.06 ㎍/㎖, respectively. The MIC of melittin was 0.06 ㎍/㎖ on S. aureus. The in vitro PAE of whole BV and isolated melittin were determined using E. coli and S. aureus. The PAE of whole BV against S. aureus were 3.45 h (1×MIC). The PAE of melittin against S. aureus was 4.35 h (1 × MIC). Also both whole BV and melittin killed S. aureus at 5 × MIC. The regrowth wasn't observed after 18 h. These results suggest that whole BV and melittin will be developed a novel antibacterial drug.
알러지 유발 단백질이 제거된 수용성 로열젤리의 생리활성 효과
김정민(Jungmin Kim),한상미(Sangmi Han),조미란(Milan Cho),이광길(Kwanggill Lee),이명렬(Myeonglyeol Lee),이만영(Manyoung Lee),우순옥(Soonok Woo),홍인표(Inpyo Hong),심하식(Hasik Sim),최용수(Youngsoo Choi) 한국양봉학회 2013 韓國養蜂學會誌 Vol.28 No.1
Royal jelly is composed of different components such as proteins, amino acid, fatty acid, and sugars. Protein is a major factor which cause allergy. We focused on water soluble royal jelly (WSRJ) that was removed allergy - inducing protein. WSRJ isolated from 70% ethanol extraction and neutralization. We confirmed that WSRJ was removed allergy - inducing protein, 48kDa and 55kDa band via SDSPAGE. 10-hyroxy-2-decenoic acid (10-HDA) content analyzed by using high performance liquid chromatography. 10-HDA content of WSRJ is 2.42g/100g, which is double compared to that of lypophilized RJ. Also, 1-0.01ug/ml concentration of WSRJ increased significantly human dermal fibroblast (HDF) proliferation for 48hr. The results indicated that WSRJ removed allergy-inducing protein increased 10-HDA content, which lead to HDF proliferation.
멜라닌세포주에서 국내산 로얄제리의 멜라닌 생성 억제 효과
한상미(Sangmi Han),이광길(Kwanggill Lee),여주홍(Joohong Yeo),이만영(Manyoung Lee) 한국양봉학회 2010 韓國養蜂學會誌 Vol.25 No.2
In this study, we have investigated the hypo-pigmentary mechanism of royal jelly form Korea in mouse melanocyte cell line, B16F1. Treatment of B16F1 cells with royal jelly markedly inhibited malanin biosynthesis in a dose-dependent manner. Decreased melanin contents occurred through the decrease of tyrosinase activity. The mRNA levels of tyrosinase were also reduced by royal jelly. These results suggest that royal jelly reduces melanin synthesis by down regulation of tyrosinase mRNA transcription and serve as a new candidate in the design of new skin-whitening or therapeutic agents.
한상미(Sangmi Han),이광길(Kwanggill Lee),여주홍(Joohong Yeo),우순옥(Soonok Woo),권해용(Haeyong Kweon),남성희(Sunghee Nam),조유영(Youyoung Jo),김원태(Wontae Kim) 한국양봉학회 2010 韓國養蜂學會誌 Vol.25 No.1
In order to investigate the potential of Korean honey for whitening cosmetics, we prepared chestnut (CH), acacia (AH), cherry blossoms (CBH), and Korean raisin tree honey (RTH). We measured their inhibitory effects on mushroom tyrosinase and melanin synthesis in B16F1. CH, CBH and RTH showed inhibitory activity against mushroom tyrosinase. They markedly inhibited mushroom tyrosinase activity at lower concentration (50% inhibitory concentration values (IC<SUB>50</SUB>) 22.5, 16.5, 19.2ug/ml, respectively) than arbutin as positive control. CH, CBH and RTH significantly prevented melanin production (IC<SUB>50</SUB> 28.6, 23.2, 25.7㎍/ml, respectively) without effects on cell viability. Therefore, this study suggests that CH, CBH and RTH may be useful as a natural skin whitening agent.
Modification of the characteristics of silkworm powder by treatment with alkaline protease
( Sungkuk Kim ),( Youyoung Jo ),( Kwanggill Lee ),( Hyunbok Kim ),( Yongsoon Kim ),( Wantaek Ju ),( Daeun Jung ),( Haeyong Kweon ) 한국잠사학회 2015 International Journal of Industrial Entomology Vol.31 No.1
Enzymatic modification of proteins is often used to increase the biological activity of materials. Silkworm powder has been investigated as a functional food resource, but no study has been performed on its modification by commercial food enzyme. Therefore, this study aimed to determine the feasibility of such modification of silkworm powder by alkaline protease. The activity of the enzyme was confirmed using an azocasein assay. Subsequently the silkworm powder was hydrolyzed by enzymatic treatment. UV visible spectrometry showed that the supernatant of silkworm powder subjected to enzymatic treatment had a stronger absorption band than the untreated powder. SDS-PAGE electrophoresis showed that the molecular weight of silkworm powder decreased on enzymatic treatment. Thus the results indicate that commercial enzymes might be used to modify the characteristics of silkworm powder.
한상미(Sangmi Han),이광길(Kwanggill Lee),여주홍(Joohong Yeo),백하주(Haju Baek) 한국양봉학회 2009 韓國養蜂學會誌 Vol.24 No.3
The aim of the study was to the determination quantitatively of the major constituents of honeybee (Apis mellifera L.) venom from Korea by liquid chromatography. Samples of honeybee venom were purchased from Chungjin, Ibee and Sigma over two consecutive beekeeping season (2007~2008). The sephadex TM75 and source 15RPC ST column was applied. Several honeybee venom constituents were separated and the content of three of them (melittin, apamine and phospholipase A₂) were determined. The chemical composition of honeybee venom from Korea were evaluated. Melittin content varied from 48.5 to 67.2 and averaged 50.7%. Apamin content camewithin a range of 2.0 to 3.2, and averaged 2.8%, and phospholipase A₂ content was between 10.8 to 14.5, averaging 12.8%. There is no statistically significant differences for the content of the main component compared standard honeybee venom to domestic honeybee venom. The quantitative composition of melittin, apamine and phospholipase A₂ may provide a basis for the development of the domestic standard for honeybee venom.
Staphylococcus aureus에 대한 국내산 꿀벌독의 항균성분 분리
한상미(Sangmi Han),이광길(Kwanggill Lee),여주홍(Joohong Yeo),권해용(Haeyong Kweon),우순옥(Soonok Woo),백하주(Haju Baek),장영채(Youngchae Chang),박관규(Kwankyu Park),Peter Molan 한국양봉학회 2008 韓國養蜂學會誌 Vol.23 No.2
Pure honeybee venom collected from Korea has been extensively used in livestock and folk medicines for a natural antibacterial agent. In the present study, we isolated and purified a novel antibacterial peptide fraction against Staphylococcus aureus from honeybee venom by liquid chromatography and mass spectrometry. The average molecular mass of the peptide was estimated to be 895.4 Da. The antibacterial activity of the peptide was evaluated in terms of Minimal inhibitory concentration. Minimal inhibitory concentration value was about 0.1μg/ml. The peptide can be considered as a potential compound for the development of new natural antibacterial agents for use in livestock and human.
한상미(Sangmi Han),이광길(Kwanggill Lee),여주홍(Joohong Yeo),권해용(Haeyong Kweon),우순옥(Soonok Woo),백하주(Haju Baek),장영채(Youngchae Chang),박관규(Kwankyu Park) 한국양봉학회 2007 韓國養蜂學會誌 Vol.22 No.2
Pure honeybee venom collected from Korea (KHBV) was assayed to find the protein composition and compared with standard honeybee venom from Sigma (SHBV), by tris-glycin gel electrophoreisis and two-dimensional gel elecrophoresis. The 10 excised spots from KHBV and SHBV were analyzed by mass spectrometry. Both pure KHBV and SHBV shows homologies in the molecular weight and amino acid sequence.
정제 봉독의 물리적 특성 및 중금속에 대한 안전성 분석
한상미(Sangmi Han),이광길(Kwanggill Lee),우순옥(Soonok Woo),김정민(Jungmin Kim),조미란(Milan Cho),백하주(Haju Baek) 한국양봉학회 2013 韓國養蜂學會誌 Vol.28 No.1
This study was carried out to characterize structure and to evaluate heavy metal safety of purified bee venom (PBV) collected using bee venom collector. According to differential thermal analysis, PBV showed degradation peak around 230°C Infrared spectrum showed double peak at 1,500~1,600cm<SUP>-1</SUP>. X-ray diffractometry analysis showed sharp diffraction peaks at 2θ=8.5° and 21.5°. Moreover, no harmful heavy metal was contained in PBV. These suggest that PBV collected from Korea may be as a very stability and safety.
오백영(Baeayoung Oh),한상미(Sangmi Han),이광길(Kwanggil Lee),백하주(Haju Baek) 한국양봉학회 2009 韓國養蜂學會誌 Vol.24 No.1
This study was conducted to identify whether honeybee venom collection by means of electrical stimulation, affected royal jelly production and costs in honeybee colonies. This experimental groups were divided from three beehive groups; R group was collected only royal jelly, RV was collected together royal jelly and bee venom at same beehive, and V was collected only bee venom. There was no difference in the yield of royal jelly at R and RV group. Physical peculiarity of royal jelly such as color, taste, flavor and form was not difference at R and RV group. The content of moisture, total protein and 10-HDA was not significantly at R and RV group. The value of production less total costs to beekeepers increased at R or V group. The results obtained demonstrated that the mean yield of royal jelly, obtained only from colonies in which venom was collected using the method of coupled electrical stimulation, was not significantly different than the yield of royal jelly recorded in control colonies.