Bioremediation of imidacloprid using Azospirillium biofertilizer and Rhizobium biofertilizer

Kavita Kulkarni,Aishwarya Chawan,Anand Kulkarni,Sandip Gharat 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.10

Imidacloprid is a pesticide used for agricultural purposes. Residue of pesticide in water and soil will affect the water and soil quality. Seepage out of imidacloprid to the ecological system could affect aquatic life as well as human. The toxic pollutants would affect the quality of agriculture run off, in turn contaminating water bodies acting as sink for these runoffs. Hence, there is need for reparation of these pollutants. Azospirillium biofertilizer and Rhizobium biofertilizer were used as adsorbent for the removal of imidacloprid. These biofertilizers have capability to reduce the harmful component as well as be useful for plant growth. Azospirillium bacteria and Rhizobium bacteria are competent for the removal of organic pollutant from wastewater. These biofertilizers maintain biological activity without any adverse effect. The adsorptive removal of imidacloprid by using Azospirillium biofertilizer and Rhizobium biofertilizer was investigated at different conditions using batch experimentation. Optimization of parameters, such as dosage, time, temperature, pH, and agitation speed, was carried out. Equilibrium adsorption was illustrated by Langmuir and Freundlich isotherms. The kinetic data was best described by intraparticle diffusion and pseudo-second-order model. Reusability study showed good removal efficiency of imidacloprid after fourth use also. The investigations show that these materials have potential to be an excellent alternative for removal of pesticides while supporting plant growth.

Mutation analysis and characterisation of F9 gene in haemophilia- B population of India

Sujayendra Kulkarni,Rajat Hegde,Smita Hegde,Suyamindra S. Kulkarni,Suresh Hanagvadi,Kusal K. Das,Sanjeev Kolagi,Pramod B. Gai,Rudragouda Bulagouda 대한혈액학회 2021 Blood Research Vol.56 No.4

Background Hemophilia B (HB) is an X-linked bleeding disorder resulting from coagulation factor IX defects. Over 3,000 pathogenic, HB-associated mutations in the F9 gene have been identified. We aimed to investigate the role of F9 variants in 150 HB patients using sequencing technology. Methods F9 gene sequences were amplified from peripheral blood-derived DNA and sequenced on an Applied Biosystems (ABI) 3500 Sanger sequencing platform. Functional and structural predictions of mutant FIX were analyzed. Results Among 150 HB patients, 102 (68%), 30 (20%), and 18 (12%) suffered from severe, moderate, and mild HB, respectively. Genetic analysis identified 16 mutations, including 3 novel mutations. Nine mutations (7 missense and 2 stop-gain) were found to be pathogenic. Only 3 mutations (c.127C>T, c.470G>A, and c.1070G>A) were associated with different severities. While 2 mutations were associated with mild HB cases (c.304C>T and c.580A>G), 2 (c.195G>A and c.1385A>G) and 3 mutations (c.223C>T, c.1187G>A, and c.1232G>A) resulted in moderate and severe disease, respectively. Additionally, 1 mutation each was associated with mild-moderate (c.*1110A>G) and mild-severe HB disease (c.197A>T), 4 mutations were associated with moderate-severe HB cases (c.314A>G, c.198A>T, c.676C>T, and c.1094C>A). FIX concentrations were lower in the mutated group (5.5±2.5% vs. 8.0±2.5%). Novel p.E66D and p.S365 mutations were predicted to be pathogenic based on changes in FIX structure and function. Conclusion Novel single nucleotide polymorphisms (SNPs) largely contributed to the pathogenesis of HB. Our study strongly suggests that population-based genetic screening will be particularly helpful to identify risk prediction and carrier detection tools for Indian HB patients.

Temporal Variation of Dominant Modes of Oscillations in the Wind Field in the Surface Boundary Layer over a Costal Indian Station

Makarand A. Kulkarni,J. R. Kulkarni,G. V. Rama,P. N. Sen 한국기상학회 2007 한국기상학회 학술대회 논문집 Vol.2007 No.-

Numerical and Experimental Analysis of Transpiration Cooling in Carbon Dioxide Atmosphere at Hypersonic Mach Numbers

V. N. Kulkarni,P. S. Kulkarni,K. P. J. Reddy 한국전산유체공학회 2003 한국전산유체공학회 학술대회논문집 Vol.2003 No.10

Finite Element Analysis of Functionally Graded Plates using Inverse Hyperbolic Shear Deformation Theory

Kulkarni, Kamlesh,Singh, Bhrigu Nath,Maiti, Dipak Kumar The Society for Aerospace System Engineering 2016 International Journal of Aerospace System Engineer Vol.3 No.1

Functionally graded materials (FGMs) are becoming very popular in various industries due to their effectiveness of the utilization of their constituent elements. However, the modelling of these materials is difficult due to the complex nature of variation of material properties across the thickness. Many shear deformation theories have been developed and employed for the analysis of such functionally graded plates (FGPs). A recently developed inverse hyperbolic shear deformation theory has been successfully employed by Grover et al. [1] for the analysis of laminated composites and sandwich plates. The objective of the study is to obtain finite element solution for the structural analysis of functionally graded plates using inverse hyperbolic shear deformation theory. Finite element analysis facilitates the analysis of complex problems such as functionally graded plates with different boundary conditions and different loadings.

“Spine Surgery Checklist”: A Step towards Perfection through Protocols

Kulkarni Arvind Gopalrao,Patel Jwalant Yogeshkumar,Asati Sanjeev,Mewara Navin 대한척추외과학회 2022 Asian Spine Journal Vol.16 No.1

Study Design: A retrospective study.Purpose: This study aimed to evaluate the effectiveness of a novel checklist that was designed specifically for the “spine-surgerysubspecialty” to reduce the incidence of some common preventable human errors and major perioperative complications in spine surgery.Overview of Literature: We propose a unique spine surgery-specific checklist that recognizes the risk factors, anticipates the possible human errors, and thus helps in preventing these errors. This checklist is associated with increased patient safety awareness, improved communication (keeps everyone updated regarding their responsibilities), reduction in the surgical claims, and reduction in the number of postoperative complications, including mortality.Methods: This retrospective pilot study was performed at single center on 858 spine surgery patients. The patients were divided into the following two groups: the study group (after implementation of the checklist [2016–2017]) and the control group (before the implementation of the checklist [2015–2016]). The incidence of common preventable human errors and major perioperative complications in spine surgeries were recorded and compared between the two groups.Results: The prevalence of wrong-level surgeries was 0%, and the overall prevalence of the preventable errors was 1.63% (7/428). The rate of adverse, near-miss, and no-harm events was 0.23% (1/428), 0.70% (3/428) and 0.70% (3/428), respectively. The preoperative, intraoperative, and postoperative errors were 0.70% (3/428), 0.23% (1/428), and 0.70 (3/428), respectively. The reoperation rate related to preventable errors reduced after the checklist was used. There were significant differences in the total preventable errors related to complications, such as infections, prolonged hospital stays, and unplanned hospital readmission/revision surgeries (p=0.001)Conclusions: The authors propose the first-of-its kind spine surgery-specific checklist that is comprehensive and involves perioperative parameters. The checklist is easy to use, safe, and effective for reducing the unforgiving errors and perioperative complications. However, its broader implementation would require validation in large, multi-center, randomized control studies.

Densitometric quantification for the validation of decolorization of Disperse Orange ERL by lichen <i>Parmelia</i> sp.

Kulkarni, Ashwini N.,Bhalkar, Bhumika N.,Khandare, Rahul V.,Kurade, Mayur B.,Jeon, Byong-Hun,Govindwar, Sanjay P. Society for Bioscience and Bioengineering, Japan 2019 Journal of bioscience and bioengineering Vol. No.

<P>Densitometric high performance thin layer chromatography (HPTLC) quantification method was developed to validate the decolorization/biotransformation of Disperse Orange ERL and dye mixture by lichen <I>Parmelia</I> sp. which release several colored compounds during decolorization process, hence unable to use colorimetric estimation. Percent decolorization of Disperse Orange ERL and dye mixture by lichen <I>Parmelia</I> sp. was observed when estimated using developed HPTLC method. Limit of detection and limit of quantification for both dyes in mixture were obtained as 0.3 and 1 μg/μl, respectively. Area of peak of control Disperse Orange ERL was reduced by 43% after 12 h, 71% after 48 h and upto 82% after 72 h of incubation. Precision and repeatability of data elucidated the % relative standard deviation less than 3 for all the values thus indicating statistically acceptable. Biodegradation of dye and mixture was confirmed with Fourier transform infrared spectroscopy analysis, i.e., altered fingerprinting spectral pattern.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Densitometric quantification of textile dyes biotransformation products. </LI> <LI> Quantification of dye in presence of released colored compounds. </LI> <LI> Reported for the first time in dye decolorization studies. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Hologram Quantitative Structure Activity Relationship Analysis of JNK Antagonists

Kulkarni, Seema A.,Madhavan, Thirumurthy The Basic Science Institute Chosun University 2015 조선자연과학논문집 Vol.8 No.2

c-Jun N-terminal kinase-3 (JNK3) is a member of the mitogen-activated protein kinase family (MAPK), and plays an important role in neurological disorders. Therefore, identification of selective JNK3 inhibitor may contribute towards neuroprotection therapies. In this work, we performed hologram quantitative structure-activity relationship (HQSAR) on a series of thiophene trisubstituted derivatives. The best predictions were obtained for HQSAR model with $q^2=0.628$ and $r^2=0.986$. Statistical parameters from the generated QSAR models indicated the data is well fitted and have high predictive ability. HQSAR result showed that atom, bond and chirality descriptors play an important role in JNK3 activity and also shows that electronegative groups is highly favourble to enhance the biological activity. Our results could be useful to design novel and selective JNK3 inhibitors.

Comparative genome analysis to identify SNPs associated with high oleic acid and elevated protein content in soybean

Kulkarni, Krishnanand P.,Patil, Gunvant,Valliyodan, Babu,Vuong, Tri D.,Shannon, J. Grover,Nguyen, Henry T.,Lee, Jeong-Dong,Belzile, F. National Research Council of Canada, Conseil natio 2018 Genome Vol. No.

<P> The objective of this study was to determine the genetic relationship between the oleic acid and protein content. The genotypes having high oleic acid and elevated protein (HOEP) content were crossed with five elite lines having normal oleic acid and average protein (NOAP) content. The selected accessions were grown at six environments in three different locations and phenotyped for protein, oil, and fatty acid components. The mean protein content of parents, HOEP, and NOAP lines was 34.6%, 38%, and 34.9%, respectively. The oleic acid concentration of parents, HOEP, and NOAP lines was 21.7%, 80.5%, and 20.8%, respectively. The HOEP plants carried both FAD2-1A (S117N) and FAD2-1B (P137R) mutant alleles contributing to the high oleic acid phenotype. Comparative genome analysis using whole-genome resequencing data identified six genes having single nucleotide polymorphism (SNP) significantly associated with the traits analyzed. A single SNP in the putative gene Glyma.10G275800 was associated with the elevated protein content, and palmitic, oleic, and linoleic acids. The genes from the marker intervals of previously identified QTL did not carry SNPs associated with protein content and fatty acid composition in the lines used in this study, indicating that all the genes except Glyma.10G278000 may be the new genes associated with the respective traits. </P>

Mapping QTLs for 100-seed weight in an interspecific soybean cross of Williams 82 (Glycine max) and PI 366121 (Glycine soja)

Kulkarni, Krishnanand P.,Asekova, Sovetgul,Lee, Dong-Ho,Bilyeu, Kristin,Song, Jong Tae,Lee, Jeong-Dong CSIRO Publishing 2017 Crop & pasture science Vol.68 No.2

<P> Seed weight can be an important component for soybean quality and yield. The objective of the present study was to identify quantitative trait loci (QTLs) for 100-seed weight by using 169 recombinant inbred lines (RILs) derived from the cross Williams 82 × PI 366121. The parental lines and RILs were grown for four consecutive years (2012-15) in the field. The seeds were harvested after maturity, dried and used to measure 100-seed weight. Analysis of variance indicated significant differences among the RILs for 100-seed weight. The environment had significant effect on seed-weight expression as indicated by the genotype × environment interaction. QTL analysis employing inclusive composite interval mapping of additive QTLs implemented in QTL IciMapping (Version 4.1) identified nine QTLs (LOD >3) on chromosomes 1, 2, 6, 8, 13, 14, 17 and 20. The individual QTLs explained phenotypic variation in the range 6.1-12.4%. The QTLs were detected in one or two environments, indicating major influence of the growing environment on seed-weight expression. Four QTLs identified in this study, qSW-02_1, qSW-06_1, qSW-13_1 and qSW-14_1, were found to be new QTLs. The findings of the study may be helpful to reveal the molecular genetic basis of the seed-weight trait in soybean. </P>