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표면 플라즈몬 공명 신호의 증폭을 위한 새로운 금 콜로이드 센서칩 제조와 특성 연구
임정혁,이경호,고광락,강신원,김재호 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1
New gold colloidal film sensor chips were employed to enhance the sensitivity of the Surface Plasmon Resonce (SPR). Au collodal solutions were synthesized, and Au colloidal films were fabricated by immobilization of colloid on vacuum deposited Au film through the coupling agent of 1,8-diaminooctane. Specific interaction of human IgG antibody to human antigen was monitored by in situ SPR measurement from both a smooth and a Au colloidal films. Au colloidal film sensor chips resulted in 27 % enhancements effect for the same reaction on SPR angle shifts compared to a smooth Au film.
Monitoring Expression of bphC Gene from Ralstonia eutropha H85O Induced by Plant Terpenes in Soil
Jung, Kyung-Ja,Kim, Byung-Hyuk,Kim, Eungbin,So, Jae-Seong,Koh, Sung-Cheol The Microbiological Society of Korea 2002 The journal of microbiology Vol.40 No.4
A PCB degrader, Ralstonia eutropha H850 was shown to induce bphC gene encoding 2,3-dihydroxy-biphenyl-1,2-dioxygenase in a carvone-amended pure culture in our previous study (Park et al.,1999). The present study was carried out to examine how plant terpenes, as natural substrates, would cause an expression of a PCB degradative gene in soil that was amended with terpenes. The population of Ralstonia eutropha H850 was maintained at least around 10$\^$8/ (CFU/g fresh soil) in the soil amended with carvone or limonene in the presence of succinate as a growth substrate at 50 th day. The gene expression was monitored by RT-PCR using total RNA directly extracted from each soil and bphC gene primers. The bphC gene expression of the seeded strain H850 was observed in the soil amended with biphenyl (4 days) but not with succinate, carvone and limonene. These results indicate that terpenes widely distributed in nature could be a potential inducing substrate for effective PCB biodegration in the soil but their bioavailability and specific induction behavior should be taken into account before PCB bioremediation implementation.
Jung, Chang-Hwa,Choi, Jin-Kyu,Yang, Yoosoo,Koh, Hyun-Ju,Heo, Paul,Yoon, Kee-Jung,Kim, Sehyun,Park, Won-Seok,Shing, Hong-Ju,Kweon, Dae-Hyuk Informa Healthcare 2012 PHARMACEUTICAL BIOLOGY Vol.50 No.9
<P><I>Context: Botulinum</I> neurotoxins (BoNTs) are popularly used to treat various diseases and for cosmetic purposes. They act by blocking neurotransmission through specific cleavage of soluble <I>N</I>-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins. Recently, several polyphenols were shown to interfere with SNARE complex formation by wedging into the hydrophobic core interface, thereby leading to reduced neuroexocytosis.</P><P><I>Objective</I>: In order to find industrially-viable plant extract that functions like BoNT, 71 methanol extracts of flowers were screened and BoNT-like activity of selected extract was evaluated.</P><P><I>Materials and methods</I>: After evaluating the inhibitory effect of 71 flower methanol extracts on SNARE complex formation, seven candidates were selected and they were subjected to SNARE-driven membrane fusion assay. Neurotransmitter release from neuronal PC12 cells and SNARE complex formation inside the cell was also evaluated. Finally, the effect of one selected extract on muscle contraction and digit abduction score was determined.</P><P><I>Results</I>: The extract of <I>Potentilla chinensis</I> Ser. (Rosaceae)(Chinese cinquefoil) flower inhibited neurotransmitter release from neuronal PC12 cells by approximately 90% at a concentration of 10 μg/mL. The extract inhibited neuroexocytosis by interfering with SNARE complex formation inside cells. It reduced muscle contraction of phrenic nerve-hemidiaphragm by approximately 70% in 60 min, which is comparable to the action of the Ca<SUP>2+</SUP>-channel blocker verapamil and BoNT type A.</P><P><I>Discussion and conclusion</I>: While BoNT blocks neuroexocytosis by cleaving SNARE proteins, the <I>Potentilla chinensis</I> extract exhibited the same activity by inhibiting SNARE complex formation. The extract paralyzed muscle as efficiently as BoNT, suggesting the potential versatility in cosmetics and therapeutics.</P>