Proliferative Pattern of Superficial Colonic Epithelium in Macroscopically Normal Colon and Rectum of Human and Baboon

Kim, Young-Jin,Kim, Dong-Yi,Cho, Chol-Gyoon,Kim, Hyun-Jong,Kim, Shin-Kon Chonnam National UniversityResearch Institute of 1993 Chonnam journal of medical sciences Vol.6 No.2

RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells

Kim, Dong-Myung,Chung, Kyung-Sook,Choi, Shin-Jung,Jung, Yu-Jin,Park, Song-Kyu,Han, Gyoon-Hee,Ha, Jae-Seok,Song, Kyung-Bin,Choi, Nam-Song,Kim, Hwan-Mook,Won, Misun,Seo, Yeon-Soo Wiley Subscription Services, Inc., A Wiley Company 2009 International journal of cancer: Journal internati Vol.125 No.11

<P>RhoB, a tumor suppressor, has emerged as an interesting cancer target, and extensive studies aimed at understanding its role in apoptosis have been performed. In our study, we investigated the involvement of RhoB-interacting molecules in apoptosis. To identify RhoB-interacting proteins, we performed yeast-two hybrid screening assays using RhoB as a bait and isolated TNFAIP1, a TNFα-induced protein containing the BTB/POZ domain. The interaction between RhoB and TNFAIP1 was demonstrated in vivo through coimmunoprecipitation studies and in vitro binding assays. RFP-TNFAIP1 was found to be partially colocalized with EGFP-RhoB. The partial colocalization of RhoB and TNFAIP1 in endosomes suggests that RhoB-TNFAIP1 interactions may have a functional role in apoptosis. TNFAIP1 elicited proapoptotic activity, while simultaneous expression of RhoB and TNFAIP1 resulted in a dramatic increase in apoptosis in HeLa cells. Furthermore, knockdown of RhoB using siRNA clearly rescued cells from apoptosis induced by TNFAIP1. This finding suggests that interactions between RhoB and TNFAIP1 are crucial for induction of apoptosis in HeLa cells. The observation of increased SAPK/JNK phosphorylation in apoptotic cells and the finding that a JNK inhibitor suppressed apoptosis indicates that SAPK/JNK signaling may be involved in apoptosis induced by RhoB-TNFAIP1 interactions. In conclusion, we found that RhoB interacts with TNFAIP1 to regulate apoptosis via a SAPK/JNK-mediated signal transduction mechanism. © 2009 UICC</P>

조류성장잠재력 시험에 의한 사량도 연안 Cochlodinium polykrikoides 적조의 제한영양염

김형철(Hyung Chul Kim),김동명(Dong Myung Kim),이대인(Dae In Lee),박청길(Chung Kil Park),김학균(Hak Gyoon Kim) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.5

우리나라 남해안에서 매년 유해 와편모조류인 Cochlodinium polykrikoides에 의한 적조가 발생하고 있는데, 본 연구에서는 남해안의 사량도 주변 해역을 대상으로 AGP 시험을 통하여 C. polykrikoides의 성장 제한영양염을 평가하였다. Macro- 및 micro-nutrients 첨가에 의한 적조 발생 단계별 C. polykrikoides의 성장은 두 가지의 서로 다른 반응으로 나타나 조류성장 제한영양염이 명확하게 확인되었다. 사량도 해역의 고정점에서 적조가 발생하기 전인 7월과 8월, 적조가 소멸한 후인 10월의 AGP 시험에서는 질산질소 (50 ㎛)와 인산인 (5 ㎛), 암모니아 질소 (50 ㎛)와 인산인 (5 ㎛)의 복합첨가에 의해 성장률이 크게 증가하였다. Macro-nutrients의 단일첨가에 대해서는 뚜렷한 성장을 나타내지 않았고 micro-nutrients도 성장을 촉진시키지 않았다. 이러한 결과로 이 시기에는 질소와 인이 동시에 C. polykrikoides의 성장 제한영양염으로 작용하였다는 것이 확인되었다. 그러나, 적조가 발생한 9월의 AGP 시험에서는 성장에 필요한 macro- 및 micro-nutrients의 결핍이 나타나지 않아 이 기간 중에 C. polykrikoides의 성장은 영양염의 제한을 받지 않는다는 것이 확인되었다. 이때 사량도 연안의 영양염 농도는 암모니아질소와 인산인 농도가 각각 24.33, 1.61 ㎛로 적조가 발생하기 전과 소멸한 후에 비해 평균 8.2, 4.8배 높은 값을 나타냈고, 질산질소 농도는 0.58 ㎛로 평균 3.3배 낮은 값을 나타냈다. 그러므로, C. polykrikoides 적조는 N, p 농도 증가에 기인한 부영양화가 진행되는 해역에서 발생할 가능성이 높다는 것을 시사한다. Algal growth potential (AGP) assay using Cochlodinium polykrikoides was conducted in Saryang Island coast where C. polykrikoides red tide occurred annually from July to October 1998. The effects of macro- and micro-nutrients on the growth of C. polykrikoides were specfically evaluated by the algal assay method. Two different types of growth response of C. polykrikoides for the addition of nutrients were clearly observed. For both before and after C, polykrikoides occurrence, the growth of C. polykrikoides was significantly stimulated by the addition of either nitrate or ammonium of 50 ㎛ with phosphate of 5 ㎛. The addition of a single nutrient had no clear effect on the growth of C. polykrikoides and the addition of trace metals, vitamins, and EDTA etc. did not stimulate the algal growth, also. This result indicates that both N and P potentially limited the growth of C. polykrikoides in this period. However, during a bloom of C. polykrikoides, the growth was unlikely to be stimulated by the addition of both macro- and micro-nutrients. At that time, the nutrient concentration of Saryang Island coast was 24.33 ㎛ for ammonium, 1.61 ㎛ for phosphate, and 0.58 ㎛ for nitrate, respectively. The concentrations of nutrients increased, on average, 8.2-fold for ammonium and 4.8-fold for phosphate, decreased 3.3-fold for nitrate compared to both before and after the red tide. This result shows that the growth of C. polykrikoides was not limited by the nutrients during the bloom in September. Therefore, our results suggest that the C. polykrikoides red tide may outbreak especially when the water is fertilized due to the increased N and P.

수직-수평온도구배법에 의한 200 mm급 사파이어 단결정 성장의 전산모사 연구

김형중 ( Hyung Joong Kim ),차필령 ( Pil Ryung Cha ),김성균 ( Seong Gyoon Kim ),문성환 ( Sung Hwan Moon ),장계원 ( Gye Won Jang ),나복기 ( Bok Kee Na ),김준환 ( Jun Hwan Kim ),김동욱 ( Dong Uk Kim ) 대한금속재료학회(구 대한금속학회) 2015 대한금속·재료학회지 Vol.53 No.1

A sapphire single crystal growth process by the Vertical-Horizontal Gradient Freezing (VHGF) method was studied by a numerical analysis technique. The heater power was controlled in order to follow the given arbitrary target heater temperature profile through the whole series of the process. Due to the influence of radiation heat transfer, the heater had spatial temperature deviation in spite of the fact that the heater power was imposed uniformly through its whole body. As a structural feature of the VHGF method, temperature at the lower part of crucible was lower than that at the upper part of crucible because it was cooler at the bottom of the furnace. Thus, the movement of the solid/liquid interface was driven in the bottom-to-top direction. (Received February 4, 2014)

일가족에 발생한 골화석증

최웅송(Oung Seung Choi),박정영(Jong Young Park),이배진(Bai Jin Lee),이정호(Jung Ho Lee),김형기(Hyung Gi Kim),최낙현(Nak Hyun Choi),박봉수(Bong Sou Park),김시찬(Si Chan Kim),변동일(Dong Il Byun),김형길(Hyung Gil Kim),정동균(Dong Gyoon 대한내과학회 1994 대한내과학회지 Vol.47 No.6

N/A Osteopetrosis (also called Albers-Schoberg's disease, Marble bones and chalk bones) is a rare hone dysplasia in which, on the basis of a failure of osteoclastic and chondroclastic resorption, the bone become exceedingly dense. Two clinically distinct forms are now recognized and have different patterns of inheritance. The milder form is transmitted as an autosomal dominant trait and the more severe form is transmitted as an autosomal recessive trait. We have experienced a familial osteopertrosis, mother and three siblinges out of six, which was thought to be a autosomal dominant inheritance. We report a case of milder form of osteopetrosis with a review of literature.

급속응고에서의 밴드조직

김성균,나형용,김동익,최회진 대한금속재료학회(대한금속학회) 1995 대한금속·재료학회지 Vol.33 No.2

The microstructural evolutions during the transition between the supersaturated solid solution and the solutal dendrite in rapid solidification were qualitatively examined using the model of banded structure formation proposed by Carrard et al., It was shown that the positive average temperature gradient at solid-liquid interface is a necessary condition for the banded structure formation, and the banded structure can not be formed during rapid undercooled solidification. Also, it was shown that the banded structure is hardly anticipated in melt-spun aluminum alloy and, on the other hand, it can be formed at the free surface side of the melt-spun Ag-Cu alloy ribbons. These predictions are in good agreement with the reported experimental results on banded structure formation.

열교환법에 의한 구리단결정 성장

김성균,나형용,김동익,최회진 대한금속재료학회(대한금속학회) 1995 대한금속·재료학회지 Vol.33 No.5

The effects of cooling velocity, melt weight and purity of copper were reported on copper single-crystal growth by Heat Exchanger Method in this paper. Copper single-crystals could be grown at the cooling velocity lower than 1℃/min. Higher purity(99.995wt%) of copper single crystal less than 7㎝ height(radius 3.5㎝) was grown when the cooling velocity was 0.34℃/min. and the height less than 3.5㎝(radius 3.5㎝) was grown with the plane front of solid-liquid interface and of semi-ellipse, and the free surface of single crystal was solidified in the end. However in the lower purity(99.93wt%) of copper, it should be solidified with plane front at the initial stage, and cell, dendrite formed finally at the cooling velocity lower than 0.5℃/min. The significant segregation were caused when the solid-liquid interface was changed from plane front to cell structure.

열교환법에 의한 실리콘 단결정 성장과정의 수치해석

김성균,나형용,김동익,최회진 대한금속재료학회(대한금속학회) 1995 대한금속·재료학회지 Vol.33 No.11

Numerical simulations including the fluid flow, conduction and radiation heat transfer have been performed using the geometry of real furnace for the single-crystal growth by heat exchanger method. The finite difference method based on the control volume approach and SIMPLE algorithm were used to solve the momentum and energy equations. Almost all parts of the furnace including the heater, insulating materials and crucible were considered in the calculation domain and the latent heat was accounted by an iterative heat evolution method. Silicon with low thermal conductivity was selected as a model material in order to compare the results with the previous report on the copper single-crystal growth. The effects of cooling rate of the heater, crucible material, crucible shape and melt weight on single-crystal growth were investigated together with the role of natural convection in melt. The optimum process conditions such as the critical cooling rate and the critical ratio of the height to the radius of crystal for the silicon single-crystal growth by heat exchanger method were determined. According to the simulations, among many parameters crucible shape was a dominant processing parameter to control single crystal growth when a thermal conductivity of the specimen was low. The lower the thermal conductivity of base material of crucible was then that of the specimen, the less the region of poly crystal formed at the edge of the crystal was.

Mechanism of Vasoactive Intestinal Polypeptide-Induced Catecholamine Secretion from the Rat Adrenal Medulla

Dong-Yoon Lim,Jae-Bong Heo,Cheol-Hee Choi,Geon-Han Lim,Yong-Gyoon Lee,Song-Hoon Oh,Il-Sik Kim,Jong-In Kim 대한생리학회-대한약리학회 1998 The Korean Journal of Physiology & Pharmacology Vol.2 No.4

<P> The present study was attempted to investigate the effect of vasoactive intestinal polypeptide (VIP) on secretion of catecholamines (CA) and to establish whether there is the existence of a noncholinergic mechanism in adrenomedullary CA secretion from the isolated perfused rat adrenal gland. The perfusion into an adrenal vein of VIP (3⁓10<SUP>⁣6 </SUP>M) for 5 min or the injection of acetylcholine (ACh, 5.32⁓10<SUP>⁣3 </SUP>M) resulted in great increases in CA secretion. Tachyphylaxis to releasing effect of CA evoked by VIP was not observed by the repeated perfusion. The net increase in adrenal CA secretion evoked by VIP still remained unaffected in the presence of atropine or chlorisondamine. However, the CA release in response to ACh was greatly inhibited by the pretreatment with atropine or chlorisondamine. The releasing effects of CA evoked by either VIP or ACh were depressed by pretreatment with nicardipine, TMB-8, and the perfusion of Ca<SUP>2⁢</SUP>-free medium. Moreover, VIP- as well as ACh-evoked CA secretory responses were markedly inhibited under the presence of (Lys<SUP>1</SUP>, Pro<SUP>2.5</SUP>, Arg<SUP>3.4</SUP>, Tyr<SUP>6</SUP>)-VIP or naloxone. CA secretory responses induced by ACh and high K<SUP>⁢</SUP> (5.6⁓10<SUP>⁣2 </SUP>M) were potentiated by infusion of VIP (3⁓10<SUP>⁣6</SUP>M for 5 min). Taken together, these experimental results indicate that VIP causes CA release in a fashion of calcium ion -dependence, suggesting strongly that there exists a noncholinergic mechanism that may be involved in the regulation of adrenomedullary CA secretion through VIP receptors in the rat adrenal gland, and that VIP may be the noncholinergic excitatory secretagogue present in the chromaffin cells.

분말 압출을 이용한 금속섬유 제조

김성균,김동익,최회진 대한금속재료학회(대한금속학회) 1995 대한금속·재료학회지 Vol.33 No.7

A new process for the metal fiber production by the powder extrusion has been developed. Being compared with the conventional processes, such as the bundle drawing method, the shatter machining method and the in-rotating water melt spinning method, the process has two distinctive advantages. One is that the fibers having a diameter below l0㎛ can be easily manufactured, the other is that the diameter, the length and the cross-sectional shape of the fibers can be controlled easily by the selection of the diameter of initial metal powders, extrusion ratios and extrusion die shapes. The silver fibers of 2㎛ wide and 30㎛ long and aluminum fibers of 5-10㎛ wide 1-3㎜ long were demonstrated.