Slide Session : OS-END-34 ; Endocrinology : Pathway Analysis Using Genome-Wide Association Study of Polycystic Ovary Syndrome

( Un Jin Shim ),( Do Kyeong Song ),( Hye Jin Lee ),( Jee Young Oh ),( Young Sun Hong ),( Han Na Kim ),( Hyung Lae Kim ),( Yeon Ah Sung ) 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in women of reproductive age, influenced by environmental and genetic factors. Genetic studies in PCOS are recently focusing on the identification of susceptibility loci through genome-wide associations studies (GWAS). However, GWAS focuses more on individual single nucleotide polymorphisms (SNPs) meeting a stringent statistical significance and most of the SNPs identified lack a functional relevance. To further elucidate the data obtained from the GWAS, pathway-based approaches are being applied. The aim of this study is to elucidate the biological pathways or gene sets involved in the pathogenesis of PCOS through pathway analysis. Methods: Two thousand unselected women of reproductive age who volunteered for evaluation of PCOS were recruited. After eliminating invalid data through the quality control procedures, 636,797 autosomal SNPs representing 1,221 individuals (432 PCOS and 789 control women) were obtained. Pathway based approach was conducted using meta-analysis gene-set enrichment of variant association (MAGENTA). Top ranking pathways or gene sets associated with PCOS were identified, and significant genes within these pathways were also determined. Results: Biological pathways related with oocyte meiosis, regulation of insulin secretion by acetylcholine and free fatty acids were significant through pathway analysis (all nominal gene-set enrichment analysis (GSEA) Ps < 0.05) (Table 1). Within these biological pathways, genes including SMC3, PLCZ1, INS, GNAQ, STXBP1, PLCB3 and PLCB2 were also identified (all Gene Ps < 0.05). Conclusions: Through pathway analysis of PCOS, biological pathways and genes associated with ovulation and insulin secretion were identified. These results might provide new insights in elucidating the pathophysiology of PCOS.

Introduction of PeryleneUnits for Enhanced Interchain Interaction in Conjugated Polymers forOrganic Photovoltaic Devices

Kim, Ji-Hoon,Kim, Hee Un,Mi, Dongbo,Jin, Sung-Ho,Shin, Won Suk,Yoon, Sung Cheol,Kang, In-Nam,Hwang, Do-Hoon American ChemicalSociety 2012 Macromolecules Vol.45 No.5

<P>A series of semiconducting copolymers, poly[2,7-(9,9′-dioctylfluorene)-<I>alt</I>-5,5′-(4′,7′-di-2-thienyl-2′,1′,3′-benzothiadiazole)](PFDTBT), poly[2,2′-(9,9-dioctyl-9<I>H</I>-fluorene-2,7-diyl)dithiophene-<I>alt</I>-5,5′-(4′,7′-di-2-thienyl-2′,1′,3′-benzothiadiazole)](PFD2TBT), and their <I>ter</I>-polymers containing peryleneunits were synthesized using Suzuki coupling polymerization. The peryleneunits were introduced to improve the charge-transport ability by enhancingthe π–π interaction between polymer chains. Theresulting polymers were characterized by <SUP>1</SUP>H NMR, elementalanalysis, DSC, and TGA. The synthesized polymers were soluble in commonorganic solvents, and formed smooth and uniform spin-coated thin films.All of the polymers studied were found to exhibit good thermal stability,losing less than 5% of their weight upon heating to approximately350 °C. Perylene- containing polymers showed higher field-effectmobilities than the corresponding PFDTBT or PFD2TBT polymers becauseof the enhanced π–π interaction between polymerchains upon the introduction of perylene units. Bulk heterojunctionsolar cells were fabricated with configuration of ITO/PEDOT:PSS/polymer:PC<SUB>71</SUB>BM/TiO<SUB><I>x</I></SUB>/Al. The devices usingthe perylene-containing polymers showed higher short-circuit currents,and fill factors than the corresponding PFDTBT or PFD2TBT devices.One of the fabricated devices using a perylene-containing copolymershowed a maximum power conversion efficiency of 3.16%, with a shortcircuit current density of 9.61 mA/cm<SUP>2</SUP>, open circuit voltageof 0.81 V, and fill factor of 41%.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/mamobx/2012/mamobx.2012.45.issue-5/ma202712r/production/images/medium/ma-2011-02712r_0013.gif'></P>

High open circuit voltage organic photovoltaic cells fabricated using 9,9′-bifluorenylidene as a non-fullerene type electron acceptor

Kim, Hee Un,Kim, Ji-Hoon,Suh, Hongsuk,Kwak, Jeonghun,Kim, Dongwook,Grimsdale, Andrew C.,Yoon, Sung Cheol,Hwang, Do-Hoon The Royal Society of Chemistry 2013 Chemical communications Vol.49 No.93

<P>We have found that 9,9′BF can be used as an electron acceptor for P3HT-based OPVs while similar devices using 4,4′BP do not show any photovoltaic effect. This can be related to the respective aromaticity and antiaromaticity of the reduced forms of 9,9′BF or 4,4′BP. The OPV device fabricated using P3HT and 9,9′BF exhibited a PCE of 2.28% with a <I>V</I><SUB>oc</SUB> of 1.07 V, a <I>J</I><SUB>sc</SUB> of 5.04 mA cm<SUP>−2</SUP>, and a FF of 0.42.</P> <P>Graphic Abstract</P><P>We have found that 9,9′BF can be used as an electron acceptor for P3HT-based OPVs while similar devices using 4,4′BP do not show any photovoltaic effect. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3cc46557h'> </P>

Methyl and Dimethyl<i>o</i>-Xylenyl-Substituted Fullerene Acceptors for Polymer Solar Cells

Kim, Hee Un,Park, Jong Baek,Grimsdale, Andrew C.,Hwang, Do-Hoon LASER PAGES PUBLISHING LTD 2015 ISRAEL JOURNAL OF CHEMISTRY Vol.55 No.9

Dibenzothiophene-Substituted Fullerene Derivative as Electron Acceptor for Polymer Solar Cells

Kim, Hee Un,Park, Jong Baek,Hwang, Do-Hoon American Scientific Publishers 2016 Journal of Nanoscience and Nanotechnology Vol.16 No.5

<P>A new fullerene derivative, [6,6]-dibenzo[b,d]thiophene-C-61-butyric acid methyl ester (DBTC61BM) was synthesized from C-60 using tosylhydrazone, and used as an electron-acceptor material for poly(3-hexylthiophene) (P3HT)-based organic photovoltaic cells. The synthesized DBTC61BM was used to modify the basic structure of [6,6]-phenyl-C-61-butyric acid methyl ester (PC61BM) by replacing the aromatic part with dibenzo[b, d] thiophene. The solubilities of DBTC61BM and PC61BM are similar; they have good solubilities in common organic solvents such as dichloromethane, chloroform, toluene, and 1,2-dichlorobenzene. The Stern-Volmer quenching constant (K-sv) of DBTC61BM was 7 x 14x10(3) M-1, and was correlated with the binding affinity between the fluorophore and a quencher. The lowest unoccupied molecular orbital energy level of DBTC61BM was -3.71 eV. The charge-carrier mobility of a P3HT: DBTC61BM blend film was determined using the space-charge-limited current method; the electron mobility value obtained for the P3HT: DBTC61BM blend film was 2 x 13 x 10(-4) cm(2) V-1 s(-1). Photovoltaic devices were fabricated using P3HT as the electron donor and DBTC61BM as the electron acceptor. Among the fabricated devices, photovoltaic cells with the structure ITO/PEDOT: PSS/P3HT: DBTC61BM/LiF/Al showed the highest power conversion efficiency, namely 3.23%, with an open-circuit voltage of 0.64 V, short-circuit-current density of 8.14 mA cm(-2), and fill factor of 0.59, under AM 1.5 G (100 mW cm(-2)) illumination.</P>

Multifunctional Amine-Containing PVA-<i>g</i>-POEM Graft Copolymer Membranes for CO₂ Capture

Kim, Do Hyun,Park, Min Su,Kim, Na Un,Ryu, Du Yeol,Kim, Jong Hak American Chemical Society 2018 Macromolecules Vol.51 No.15

<P>CO<SUB>2</SUB> separation technology using polymeric membranes has emerged as a viable solution to mitigate the rapidly increasing anthropogenic CO<SUB>2</SUB> emissions, which are directly linked to global warming and climate anomalies. However, in order to improve the gas separation performance, an intrinsic problem of polymeric membranes, i.e., a trade-off relationship between permeability and selectivity, needs to be addressed. In this study, a solid-state facilitated transport membrane is prepared by blending CO<SUB>2</SUB>-philic, amine-compatible poly(vinyl alcohol)-<I>g</I>-poly(oxyethylene methacrylate) (PVA-<I>g</I>-POEM) graft copolymer and diethylenetriamine (DETA) carriers. The graft copolymer consisting of PVA main chains and POEM side chains is synthesized via one-pot free radical polymerization and used as a polymer matrix. With the incorporation of 10 wt % of DETA into the polymer, the membrane exhibits a high CO<SUB>2</SUB> permeance of 402.5 GPU (1 GPU = 10<SUP>-6</SUP> cm<SUP>3</SUP>(STP)/(s·cm<SUP>2</SUP>·cmHg)) and CO<SUB>2</SUB>/N<SUB>2</SUB> selectivity of 52.1. The enhanced performance of the membrane results from multifunctional amine carriers in the graft copolymer owing to facilitated transport of CO<SUB>2</SUB> and an increase in the crystallinity of the polymer. DETA acts as a seed for crystallization of PVA chains at a low loading, which leads to a reduced N<SUB>2</SUB> permeance. The membrane exhibits good stability without any performance degradation over 2 weeks in the solid state. The superior separation property of the PVA-<I>g</I>-POEM membrane containing DETA distinguishes this facilitated transport membrane from other similar membranes and commercial membranes.</P> [FIG OMISSION]</BR>

Effects of Catechins and Wheat Bran on the Beef Color in the Late Fattening Period of Hanwoo Steers

Kim, Do-Hyung,Oh, Young-Kyoon,Jang, Sun-Sik,Kwon, Eung-Gi,Seo, Yong-Joo,Ok, Ji-Un,Park, Keun-Kyu,Lee, Sung-Sill,Kim, Kyoung-Hoon Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.6

This study was conducted to evaluate the effects of dietary supplementation of catechins and wheat bran on growth performance, carcass characteristics and meat color using forty-eight Hanwoo steers. Each steer was randomly assigned to one of four treatments. Treatments were as follows: control, catechins, wheat bran, and catechins+wheat bran (CW). At the end of the feeding trial, blood samples were collected for analysis of hemoglobin and iron concentration, and then steers were slaughtered at a commercial abattoir. Catechins, wheat bran, and CW had no effect on growth performance and carcass characteristics. Percentage of myoglobin in M. longissimus dorsi was not affected by treatments. Percentage of oxymyoglobin was increased (p<0.05) by CW and maintained at the highest level (p<0.05) for CW during meat display time up to 24 h. Percentage of metmyoglobin was the lowest (p<0.05) for CW between 5 and 24 h during display time. Although blood iron concentration was not affected by treatments, total concentration of muscle iron was the lowest (p<0.05) for CW compared with that of other treatments. Therefore, the results of the present study suggest that CW may provide good protection against oxidation of myoglobin, and might be an effective dietary supplement for improving meat color in the late fattening period of Hanwoo steers.

Highly efficient and spectrally stable white organic light-emitting diodes using new red heteroleptic Iridium(III) complexes

Kim, Hee Un,Jang, Jae-Ho,Park, Hea Jung,Jung, Byung Jun,Song, Wook,Lee, Jun Yeob,Hwang, Do-Hoon Elsevier 2018 Dyes and pigments Vol.149 No.-

<P><B>Abstract</B></P> <P>Highly efficient red phosphorescent iridium(III) complexes, (MPQ)<SUB>2</SUB>Ir(Pppy) and (MPQ)<SUB>2</SUB>Ir(TMSppy) based on 4-methyl-2-phenylquinoline (MPQ) as a cyclometalated main ligand and 2-[(1,1′-biphenyl)4-yl]pyridine (Pppy) or 2-[4-(trimethylsilyl)phenyl]pyridine (TMSppy) as a second cyclometalated ligand were synthesized for use in organic light-emitting diodes (OLEDs). The iridium(III) complex with MPQ and acetylacetone (acac) as an ancillary ligand, (MPQ)<SUB>2</SUB>Ir(acac), was also synthesized for comparison. The geometrical configuration of the heteroleptic complex (MPQ)<SUB>2</SUB>Ir(<I>acac</I>)is <I>N</I>,<I>N</I>-trans, whereas (MPQ)<SUB>2</SUB>Ir(Pppy) and (MPQ)<SUB>2</SUB>Ir(TMSppy)show all-<I>facial</I> geometries. Among the iridium(III) complexes, red OLEDs using the (MPQ)<SUB>2</SUB>Ir(Pppy) exhibited significantly more improved luminance and external quantum efficiency with markedly prevented concentration self-quenching at high doping concentration by introduction of Pppy. Moreover, a high maximum external quantum efficiency (EQE<SUB>max</SUB>) of 28.0% with a high color-rendering index (CRI) of 81 was achieved in the white OLEDs (WOLED) fabricated using the bis[2-(4,6-difluorophenyl)pyridinato-C<SUP>2</SUP>,<I>N</I>](picolinato)iridium(III) [FIrpic], tris(2-phenylpyridine)iridium(III) [Ir(ppy)<SUB>3</SUB>], and (MPQ)<SUB>2</SUB>Ir(Pppy), and it is one of the highest values among the reported three primary color component WOLED. Also, the WOLED fabricated using the (MPQ)<SUB>2</SUB>Ir(Pppy) showed excellent spectral stability at different voltages.</P> <P><B>Highlights</B></P> <P> <UL> <LI> New red phosphorescent Ir(III) complexes, (MPQ)<SUB>2</SUB>Ir(Pppy) and (MPQ)<SUB>2</SUB>Ir(TMSppy) were synthesized. </LI> <LI> (MPQ)<SUB>2</SUB>Ir(Pppy) exhibited high maximum external quantum efficiency of 28.0% in WOLED. </LI> <LI> (MPQ)<SUB>2</SUB>Ir(Pppy) showed excellent spectral stability at different voltages in WOLED. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Carbazole-containing fullerene derivatives for P3HT-based bulk-heterojunction solar cells

Kim, Hee Un,Mi, Dongbo,Kim, Ji-Hoon,Park, Jong Baek,Yoon, Sung Cheol,Yoon, Ung Chan,Hwang, Do-Hoon Elsevier 2012 Solar energy materials and solar cells Vol.105 No.-

Incorporation of Pyrene Units to Improve Hole Mobility in Conjugated Polymers for Organic Solar Cells

Kim, Ji-Hoon,Kim, Hee Un,Kang, In-Nam,Lee, Sang Kyu,Moon, Sang-Jin,Shin, Won Suk,Hwang, Do-Hoon American Chemical Society 2012 Macromolecules Vol.45 No.21

<P>Solution-processable semiconducting copolymers, poly[<I>N</I>-9′-heptadecanyl-2,7-carbazole-<I>alt</I>-5,5′-(4′,7′-di-2-thienyl-2′,1′,3′-benzothiadiazole)] (PCDTBT) and poly[4,8-bis(2-ethylhexyl-2-thenyl)-benzo[1,2-<I>b</I>:4,5-<I>b</I>′]dithiophene-<I>alt</I>-5,5′-(4′,7′-di-2-thienyl-2′,1′,3′-benzothiadiazole)] (PBDTDTBT), and their pyrene-containing terpolymers were synthesized using Suzuki or Stille coupling. Pyrene units were introduced to improve the charge-transporting abilities of the polymers. The resulting polymers were found to be soluble in common organic solvents and formed smooth and uniform spin-coated thin films. They also exhibited good thermal stability and lost <5% of their weight upon heating to ∼350 °C. Solution-processed field-effect transistors fabricated using these polymers showed p-type organic thin-film transistor characteristics. The pyrene-containing terpolymers showed higher field-effect mobilities than their corresponding parent polymers, and their mobility increased with increasing pyrene content. Furthermore, they had lower HOMO energy levels than the corresponding PCDTBT or PBDTDTBT polymers. Bulk heterojunction solar cells with an ITO/PEDOT:PSS/polymer:PC<SUB>71</SUB>BM/Ca/Al configuration fabricated using the pyrene-containing polymers had higher power conversion efficiencies than those using the corresponding parent polymers.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/mamobx/2012/mamobx.2012.45.issue-21/ma301877q/production/images/medium/ma-2012-01877q_0013.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ma301877q'>ACS Electronic Supporting Info</A></P>