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RISS 인기검색어
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- 저자 : Kempe, Andrea (검색결과 44 건)
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Stealth Poly(2-oxazoline) Nanoparticles for Biomedical Applications
Kristian Kempe 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
We report the preparation of functional and biocompatible poly(2-oxazoline) (POx)-based nanoparticles of different morphologies and discuss their bio-nano interactions. POx are a highly functional polymer class, which provides access to tailor-made polymers with tuneable physicochemical properties. The water-soluble variants are highly biocompatible, non-immunogenic and show a stealth behavior. Thus, they are highly interesting as shell materials for diverse nanoparticles used in a biological context. In this presentation we will highlight our recent efforts in fabricating spherical and rod-shaped POx nanoparticles through, e.g. emulsion processes, surface coatings of inorganic nanoparticles and crystallization-driven self-assembly (CDSA). The potential to modulate the interaction of these nanoparticles with biological entities through variation of size, shape and polymer/surface modifications will be discussed.
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L?hle, Matthias,Hermann, Andreas,Glass, Hannes,Kempe, Andrea,Schwarz, Sigrid C,Kim, Jeong Beom,Poulet, Claire,Ravens, Ursula,Schwarz, Johannes,Sch?ler, Hans R,Storch, Alexander AlphaMed Press 2012 Stem cells Vol.30 No.3
<P>Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) by retroviral overexpression of the transcription factors Oct4, Sox2, Klf4, and c-Myc holds great promise for the development of personalized cell replacement therapies. In an attempt to minimize the risk for chromosomal disruption and to simplify reprogramming, several studies demonstrated that a reduced set of reprogramming factors is sufficient to generate iPSC, albeit at lower efficiency. To elucidate the influence of factor reduction on subsequent differentiation, we compared the efficiency of neuronal differentiation in iPSC generated from postnatal murine neural stem cells with either one (Oct4; iPSC(1F-NSC) ), two (Oct4, Klf4; iPSC(2F-NSC) ), or all four factors (iPSC(4F-NSC) ) with those of embryonic stem cells (ESCs) and iPSC produced from fibroblasts with all four factors (iPSC(4F-MEF) ). After 2 weeks of coculture with PA6 stromal cells, neuronal differentiation of iPSC(1F-NSC) and iPSC(2F-NSC) was less efficient compared with iPSC(4F-NSC) and ESC, yielding lower proportions of colonies that stained positive for early and late neuronal markers. Electrophysiological analyses after 4 weeks of differentiation identified functional maturity in neurons differentiated from ESC, iPSC(2F-NSC) , iPSC(4F-NSC) , and iPSC(4F-MEF) but not in those from iPSC(1F-NSC) . Similar results were obtained after hematoendothelial differentiation on OP9 bone marrow stromal cells, where factor-reduced iPSC generated lower proportions of colonies with hematoendothelial progenitors than colonies of ESC, iPSC(4F-NSC) , and iPSC(4F-MEF) . We conclude that a reduction of reprogramming factors does not only reduce reprogramming efficiency but may also worsen subsequent differentiation and hinder future application of iPSC in cell replacement therapies.</P>
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Kim, I.B.,Ferke, P.R.,Powers, W.J.,Stein, H.H.,Van Kempe, T.A.T.G. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.8
The objective of this study was to investigate the effects of different sources of Ca and P on urine and ileal digesta pH, and ammonia ($NH_{3}$), methane ($CH_{4}$), and odor emission. In experiment 1, eight pigs (commercial three-way cross; initial BW 67$\pm$3 kg) were arranged in a repeated 4$\times$4 Latin Square design. All pigs were equipped with a T-cannula in the distal ileum. Four corn-soybean meal based diets were formulated. Diet 1 was the control in which dicalcium phosphate (DCP) and limestone ($CaCO_{3}$) were used as the sources of inorganic P and Ca. In Diets 2 and 3, ${H_{3}}{PO_{4}}$, monocalcium phosphate (MCP), and $CaSO_{4}$replaced DCP and $CaCO_{3}$ as the inorganic sources of P and Ca. Diet 4 was similar to Diet 1 except that it was fortified with HCl to provide an acid load similar to that of diet 2. Urine and ileal digesta pH were determined in pigs fed each of these diets. In Exp. 1, urine pH decreased (p<0.05) in animals consuming diets containing ${H_{3}}{PO_{4}}$-$CaSO_{4}$ (5.85$\pm$0.38) and MCP-$CaSO_{4}$(5.73$\pm$0.30) compared with the DCP-$CaCO_{3}$ diet (6.89$\pm$0.24). In the pigs consuming ${H_{3}}{PO_{4}}$-$CaSO_{4}$, ileal digesta pH decreased compared with the control (5.52$\pm$0.28 vs. 6.66$\pm$0.17; p<0.05). Based on the results of Exp. 1, a total of four trials were performed in environmental chambers for determining how $NH_{3}$, $NH_{4}$, and odor were affected by the different dietary Ca and P sources (Exp. 2). In Exp. 2, pigs fed the ${H_{3}}{PO_{4}}$-$CaSO_{4}$ diet had decreased (30%) $NH_{3}$ emissions compared with the control (p<0.05). Also, a combination of MCP-$CaCO_{3}$-$CaCl_{12}$ decreased $NH_{3}$ emission by 15% (p<0.05). Emission of $CH_{4}$ was decreased only with the ${H_{3}}{PO_{4}}$-$CaSO_{4}$ diet with 14% (p<0.05). Odorant emission of phenolics and volatile fatty acids increased roughly three-fold with the DCP-$CaSO_{4}$ diet but was not affected by other test diets. In conclusion, acidogenic Ca and P sources in swine diets can decrease the urinary pH and reduce $NH_{3}$ and $CH_{4}$ emission from swine facilities.
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Kemp, Michael G.,Gaddameedhi, Shobhan,Choi, Jun-Hyuk,Hu, Jinchuan,Sancar, Aziz American Society for Biochemistry and Molecular Bi 2014 The Journal of biological chemistry Vol.289 No.38
<P>Ultraviolet (UV) photoproducts are removed from genomic DNA by dual incisions in humans in the form of 24- to 32-nucleotide-long oligomers (canonical 30-mers) by the nucleotide excision repair system. How the small, excised, damage-containing DNA oligonucleotides (sedDNAs) are processed in cells following the dual incision event is not known. Here, we demonstrate that sedDNAs are localized to the nucleus in two biochemically distinct forms, which include chromatin-associated, transcription factor II H-bound complexes and more readily solubilized, RPA-bound complexes. Because the nuclear mobility and repair functions of transcription factor II H and RPA are influenced by post-incision gap-filling events, we examined how DNA repair synthesis and DNA ligation affect sedDNA processing. We found that although these gap filling activities are not essential for the dual incision/sedDNA generation event <I>per se</I>, the inhibition of DNA repair synthesis and ligation is associated with a decrease in UV photoproduct removal rate and an accumulation of RPA-sedDNA complexes in the cell. These findings indicate that sedDNA processing and association with repair proteins following the dual incisions may be tightly coordinated with gap filling during nucleotide excision repair <I>in vivo</I>.</P>
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Cancer nanotechnology: current status and perspectives
Kemp Jessica A.,Kwon Young Jik 나노기술연구협의회 2021 Nano Convergence Vol.8 No.34
Modern medicine has been waging a war on cancer for nearly a century with no tangible end in sight. Cancer treatments have significantly progressed, but the need to increase specificity and decrease systemic toxicities remains. Early diagnosis holds a key to improving prognostic outlook and patient quality of life, and diagnostic tools are on the cusp of a technological revolution. Nanotechnology has steadily expanded into the reaches of cancer chemotherapy, radiotherapy, diagnostics, and imaging, demonstrating the capacity to augment each and advance patient care. Nanomaterials provide an abundance of versatility, functionality, and applications to engineer specifically targeted cancer medicine, accurate early-detection devices, robust imaging modalities, and enhanced radiotherapy adjuvants. This review provides insights into the current clinical and pre-clinical nanotechnological applications for cancer drug therapy, diagnostics, imaging, and radiation therapy.
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[Invited Lecture Ⅳ] Improving Outcomes for All Vulnerable Families with Young Children
Lynn Kemp 한국간호과학회 2013 한국간호과학회 학술대회 Vol.2013 No.10
Children born to families experiencing disadvantage do not have the same opportunity for good health as those living in more advantaged circumstances. In Australia and internationally there is increased interest in developing interventions that will reduce health inequalities and recognition that early childhood sustained nurse home visiting interventions offer the greatest potential for long term change. In response many countries have invested in home visiting program for families with specific risk factors (such as being a first-time teenaged mother). This targeted investment, however, will not produce change in the levels of vulnerability in whole populations. Families are, or may become vulnerable for many reasons and at many times through their experience of parenting with up to 40 percent of families in the population requiring additional support either some or all of the time. It is now recognised that to make a difference to improve outcomes for whole communities and populations, children"s access to positive early experiences needs to be tackled along the whole social gradient, with services and support being proportionally targeted to the needs and capacities of all families. This paper will discuss the essential role that nurses working through universal health systems have in supporting families when they are experiencing vulnerabilities. The paper will focus on the Maternal Early Childhood Sustained Home-visiting (MECSH) program of sustained nurse home visiting for vulnerable families at risk of poorer maternal and child health and development outcomes. MECSH draws together the best available evidence on the importance of the early years, children"s health and development, the types of support parents need, parent-infant interaction and holistic, ecological approaches to supporting families to establish the foundations of a positive life trajectory for their children. Targeting 20-25 percent of the population who need ongoing support, MECSH is delivered as part of an integrated approach to services for young children and their families. To encompass the range of needs encountered, it is broad based and multi-faceted, and also aims to exploit and integrate with resources available in the wider community. The program is delivered by public health nurses who are embedded within universal child and family health services, as part of a comprehensive program that includes group activities, and engagement with and referral to other services. MECSH is explicitly designed to support and develop child and family health nursing services to provide effective prevention and early intervention programs to achieve improved outcomes for all vulnerable families.
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