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Investigation of the Structural Behavior of an Innovative Steel Open-web Floor System
Huanqiang Luan,Kejian Ma,Ying Qin,Zhi-Hua Chen,Yanhui Wei 한국강구조학회 2017 International Journal of Steel Structures Vol.17 No.4
An experimental study was conducted on a steel open-web floor system, in which the top and bottom chords are connected by a steel square tube to form a vertical web member. A full-scale flexural test was performed to evaluate the flexural performance and failure mechanisms of the floor system. The steel open-web floor structure demonstrated a satisfactory composite behavior, and the loading capacity increased to 2.6 times the working load. A parametric study was performed to examine the effects of three geometric properties. Increasing the size of the cross sections exerted a significant effect on stiffness, and the load capacity of the open web floor system was enhanced significantly. Results show that the design method and philosophy of the open-web floor structure are in accordance with Eurocode 4 and the Chinese Code for the Design of Steel Structures.
Nucleomodulin BspJ as an effector promotes the colonization of Brucella abortus in the host
Zhongchen Ma,Shuifa Yu,Kejian Cheng,Yuhe Miao,Yimei Xu,Ruirui Hu,Wei Zheng,Jihai Yi,Huan Zhang,Ruirui Li,Zhiqiang Li,Yong Wang,Chuangfu Chen 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.1
Background: Brucella infection induces brucellosis, a zoonotic disease. The intracellular circulation process and virulence of Brucella mainly depend on its type IV secretion system (T4SS) expressing secretory effectors. Secreted protein BspJ is a nucleomodulin of Brucella that invades the host cell nucleus. BspJ mediates host energy synthesis and apoptosis through interaction with proteins. However, the mechanism of BspJ as it affects the intracellular survival of Brucella remains to be clarified. Objectives: To verify the functions of nucleomodulin BspJ in Brucella's intracellular infection cycles. Methods: Constructed Brucella abortus BspJ gene deletion strain (B. abortus ΔBspJ) and complement strain (B. abortus pBspJ) and studied their roles in the proliferation of Brucella both in vivo and in vitro. Results: BspJ gene deletion reduced the survival and intracellular proliferation of Brucella at the replicating Brucella-containing vacuoles (rBCV) stage. Compared with the parent strain, the colonization ability of the bacteria in mice was significantly reduced, causing less inflammatory infiltration and pathological damage. We also found that the knockout of BspJ altered the secretion of cytokines (interleukin [IL]-6, IL-1β, IL-10, tumor necrosis factor-α, interferon-γ) in host cells and in mice to affect the intracellular survival of Brucella. Conclusions: BspJ is extremely important for the circulatory proliferation of Brucella in the host, and it may be involved in a previously unknown mechanism of Brucella's intracellular survival.