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RISS 인기검색어
- 검색키워드
- 저자 : Kailasa S. Kumar (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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Kumar, Kailasa S.,Lee, Hee-Gu,Yoo, Dong-Jin,Kang, Seong-Ho Korean Chemical Society 2008 Bulletin of the Korean Chemical Society Vol.29 No.1
A microchip-based capillary gel electrophoresis (MCGE) technique was developed for the ultra-fast detection and differentiation of Candidatus Mycoplasma haemominutum (Candidatus M. haemominutum, California strain) and Mycoplasma haemofelis (M. haemofelis, Ohio strain) in Korean feral cats through the application of programmed field strength gradients (PFSG) in a conventional glass double-T microchip. The effects of the poly (ethyleneoxide) (PEO) concentration and electric field strength on the separation of DNA fragments were investigated. The PCR-amplified products of Candidatus M. haemominutum (202-bp) and M. haemofelis (273-bp) were analyzed by MCGE within 75 s under a constant applied electric field of 117.6 V/cm and a sieving matrix of 0.3% PEO (Mr 8 000 000). When the PFSG was applied, MCGE analysis generated results 6.8-times faster without any loss of resolution or reproducibility. The MCGE-PFSG technique was also applied to eleven samples selected randomly from 33 positive samples. The samples were detected and differentiated within 11 s. The analysis time of the MCGE-PFSG technique was approximately 980-times faster than that using conventional slab gel electrophoresis.
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Kailasa S. Kumar,이희구,유동진,강성호 대한화학회 2008 Bulletin of the Korean Chemical Society Vol.29 No.1
A microchip-based capillary gel electrophoresis (MCGE) technique was developed for the ultra-fast detection and differentiation of Candidatus Mycoplasma haemominutum (Candidatus M. haemominutum, California strain) and Mycoplasma haemofelis (M. haemofelis, Ohio strain) in Korean feral cats through the application of programmed field strength gradients (PFSG) in a conventional glass double-T microchip. The effects of the poly (ethyleneoxide) (PEO) concentration and electric field strength on the separation of DNA fragments were investigated. The PCR-amplified products of Candidatus M. haemominutum (202-bp) and M. haemofelis (273-bp) were analyzed by MCGE within 75 s under a constant applied electric field of 117.6 V/cm and a sieving matrix of 0.3% PEO (Mr 8 000 000). When the PFSG was applied, MCGE analysis generated results 6.8-times faster without any loss of resolution or reproducibility. The MCGE-PFSG technique was also applied to eleven samples selected randomly from 33 positive samples. The samples were detected and differentiated within 11 s. The analysis time of the MCGE-PFSG technique was approximately 980-times faster than that using conventional slab gel electrophoresis.
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Review of nanomaterials as sorbents in solid-phase extraction for environmental samples
Azzouz, Abdelmonaim,Kailasa, Suresh Kumar,Lee, Sang Soo,J. Rascó,n, André,s,Ballesteros, Evaristo,Zhang, Ming,Kim, Ki-Hyun Elsevier 2018 Trends in analytical chemistry Vol.108 No.-
<P><B>Abstract</B></P> <P>Anthropogenic organic contaminants (AOCs) are found to exert significant impacts on the human ecosystem, even at low or trace-level concentrations. To meet the growing demand for their quantitation in diverse environmental media, the use of preconcentration approaches (such as solid phase extraction) has become an essential component to practically upgrade both procedural efficiency and the analytical sensitivity. Nanomaterials (NMs) are realized as excellent candidates for proper sorption media because of their unique structural and surface properties with noticeably enhanced sorption capability towards contaminants. This review explores the use of various NMs (metallic and mixed oxide nanoparticles (NPs), carbon NMs (fullerenes, carbon nanotubes, graphene, and graphene oxide), polymer-based nanocomposites (organic polymers, inorganic and hybrid polymers, molecularly imprinted polymers, and dendrimers), and silicon/magnetic NPs) as potential sorbents for analytical applications. In this review, the distinctive features of NM-based sorptive extraction techniques are examined comprehensively with the discussion on their future prospects and key challenges.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Applications of nanomaterials in sorptive extraction techniques are overviewed. </LI> <LI> Extraction efficiency of sorptive extraction was improved using nanomaterials. </LI> <LI> This review highlights the integration of nanomaterials with sorptive extraction techniques. </LI> <LI> The prospects of nanomaterials technology in trace level analysis are described. </LI> </UL> </P>
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Goud, K. Yugender,Kailasa, Suresh Kumar,Kumar, Vanish,Tsang, Yiu Fai,Lee, S.E.,Gobi, K. Vengatajalabathy,Kim, Ki-Hyun Elsevier 2018 Biosensors & bioelectronics Vol.121 No.-
<P><B>Abstract</B></P> <P>Nanomaterial-embedded sensors have been developed and applied to monitor various targets. Mycotoxins are fungal secondary metabolites that can exert carcinogenic, mutagenic, teratogenic, immunotoxic, and estrogenic effects on humans and animals. Consequently, the need for the proper regulation on foodstuff and feed materials has been recognized from times long past. This review provides an overview of recent developments in electrochemical sensors and biosensors employed for the detection of mycotoxins. Basic aspects of the toxicity of mycotoxins and the implications of their detection are comprehensively discussed. Furthermore, the development of different molecular recognition elements and nanomaterials required for the detection of mycotoxins (such as portable biosensing systems for point-of-care analysis) is described. The current capabilities, limitations, and future challenges in mycotoxin detection and analysis are also addressed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Mycotoxins are fungal secondary metabolites that exert toxic effects on humans and animals. </LI> <LI> Exploration of mycotoxins is an interdisciplinary research area that has progressed intensively. </LI> <LI> Different nanomaterials (NMs) were used for the detection of small molecules, including mycotoxins. </LI> <LI> Here the recent developments of NM-based EC biosensors are described for mycotoxin detection. </LI> <LI> The role of EC sensors is assessed for combined applications with diverse recognition elements. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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